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The map label for this gene is ahpF [H]

Identifier: 124027077

GI number: 124027077

Start: 162184

End: 163218

Strand: Direct

Name: ahpF [H]

Synonym: Hbut_0180

Alternate gene names: 124027077

Gene position: 162184-163218 (Clockwise)

Preceding gene: 124027076

Following gene: 124027079

Centisome position: 9.73

GC content: 55.75

Gene sequence:

>1035_bases
TTGGCCGAGCAGACTGGGCAGACGGTAGGACGGTCCGTTGAGGGTGAGGTTGAGAGGCACGATGTTATAATAGTCGGTGC
CGGCATGGCGGGGCTGACGGCGGCGCTCTACCTTGCAAGGCAGGGTGTTGACGTGCTGGTTGTGAGCCGCGATCTCGGTG
GCCAGCTTATGCAGGCAACTATTATCGAGAACTACCCGGGGATAGAGGCTGTTAAGGGACCAGAGCTGGCAATGAGGGTT
GAAAAGCAAGCTAGGACCTTTGGTGCAAAGTTTAGGTATGGTGAGCAGATAGTCAGGATTGACAAGCTCGAGGACGGCAC
GTTCCTCCTTGAGAGCAACCGGGGTAGGAGGTACCAGGCGGAAGTGGTCATACTAGCTTTTGGCAAGACCCCGAAGAGGA
TGAACATACCCGGCGAGGATGAGTACGATGGTCGTGGCGTAAGCTACTGCACCATCTGCGACGGTCCACTATTCCGCGGC
AGGAACACAGCTGTCGTGGGCATAGGGGATCAGGGTCTTGAGGCGGTTGCGCTCTTAGCAGGGCTCTGCCCCAAGGTCTA
CTACGTGACTCCAACCAAGCTCTTTGGAGAGCCAGACCTAATCGCTAGGATAAAGTCTGCACCCAACGTGGAGATCCTTG
AGGAGCATGAGCCGGTAAAAGTGCTAGGTGATGGGAAGAGGGTTACCGGCTTAGTGGTACGCAACAAGAAGACTGGCGAG
GAGAAAACCCTGGATGTCGAGGGGATATTTGTCGAGCTCGGCTACGAGGCTAGGACAGAGTTTGTAAAGCACCTAGTAGA
CCTCAATGAGAACGGCGAGATAATAACTGGCAAGCTCGGCGAGACGAGGACGCCAGGGCTATTTGCGGCGGGCGACGTTA
CCGATGTACCGTTCAAGCAGGCCATTATCTCCGCTGGCGACGCTGCCAAGGCGGCACTAGCAGCGTTCAACTACCTGCAG
AAAAGGCGTGGCCGTAACGTCACGATAAGGGTGACTGGAAGAAGACTGGTAGGGTTAAGCCAAAAACGCAGATGA

Upstream 100 bases:

>100_bases
CTCCTACTAGGTTGCCTACACAACGTATATACCGAGCGAGGGTACAGCCTAACCGTCTTTACCGGACGTTATAGAGGCTC
CTAGCTCCGGGGTGTAGGGC

Downstream 100 bases:

>100_bases
GACTTGGGCTGAAGAAGAGGGAGGGCTAGAGACCCTCCTCCTTCCTGATCTTCTCGGCGTACTCCTCGGGGCTAAGCAGC
TTCTCCAGCTCGGAGCGATC

Product: thioredoxin reductase

Products: thioredoxin disulfide; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MAEQTGQTVGRSVEGEVERHDVIIVGAGMAGLTAALYLARQGVDVLVVSRDLGGQLMQATIIENYPGIEAVKGPELAMRV
EKQARTFGAKFRYGEQIVRIDKLEDGTFLLESNRGRRYQAEVVILAFGKTPKRMNIPGEDEYDGRGVSYCTICDGPLFRG
RNTAVVGIGDQGLEAVALLAGLCPKVYYVTPTKLFGEPDLIARIKSAPNVEILEEHEPVKVLGDGKRVTGLVVRNKKTGE
EKTLDVEGIFVELGYEARTEFVKHLVDLNENGEIITGKLGETRTPGLFAAGDVTDVPFKQAIISAGDAAKAALAAFNYLQ
KRRGRNVTIRVTGRRLVGLSQKRR

Sequences:

>Translated_344_residues
MAEQTGQTVGRSVEGEVERHDVIIVGAGMAGLTAALYLARQGVDVLVVSRDLGGQLMQATIIENYPGIEAVKGPELAMRV
EKQARTFGAKFRYGEQIVRIDKLEDGTFLLESNRGRRYQAEVVILAFGKTPKRMNIPGEDEYDGRGVSYCTICDGPLFRG
RNTAVVGIGDQGLEAVALLAGLCPKVYYVTPTKLFGEPDLIARIKSAPNVEILEEHEPVKVLGDGKRVTGLVVRNKKTGE
EKTLDVEGIFVELGYEARTEFVKHLVDLNENGEIITGKLGETRTPGLFAAGDVTDVPFKQAIISAGDAAKAALAAFNYLQ
KRRGRNVTIRVTGRRLVGLSQKRR
>Mature_343_residues
AEQTGQTVGRSVEGEVERHDVIIVGAGMAGLTAALYLARQGVDVLVVSRDLGGQLMQATIIENYPGIEAVKGPELAMRVE
KQARTFGAKFRYGEQIVRIDKLEDGTFLLESNRGRRYQAEVVILAFGKTPKRMNIPGEDEYDGRGVSYCTICDGPLFRGR
NTAVVGIGDQGLEAVALLAGLCPKVYYVTPTKLFGEPDLIARIKSAPNVEILEEHEPVKVLGDGKRVTGLVVRNKKTGEE
KTLDVEGIFVELGYEARTEFVKHLVDLNENGEIITGKLGETRTPGLFAAGDVTDVPFKQAIISAGDAAKAALAAFNYLQK
RRGRNVTIRVTGRRLVGLSQKRR

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=309, Percent_Identity=35.9223300970874, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1787114, Length=317, Percent_Identity=34.0694006309148, Blast_Score=156, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321898, Length=328, Percent_Identity=35.9756097560976, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6320560, Length=315, Percent_Identity=35.8730158730159, Blast_Score=165, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9

Molecular weight: Translated: 37458; Mature: 37326

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQTGQTVGRSVEGEVERHDVIIVGAGMAGLTAALYLARQGVDVLVVSRDLGGQLMQAT
CCCCCCHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
IIENYPGIEAVKGPELAMRVEKQARTFGAKFRYGEQIVRIDKLEDGTFLLESNRGRRYQA
HHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCEEEE
EVVILAFGKTPKRMNIPGEDEYDGRGVSYCTICDGPLFRGRNTAVVGIGDQGLEAVALLA
EEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHH
GLCPKVYYVTPTKLFGEPDLIARIKSAPNVEILEEHEPVKVLGDGKRVTGLVVRNKKTGE
HCCCCEEEECCHHHCCCCHHHHHHCCCCCEEEEECCCCEEEEECCCEEEEEEEECCCCCC
EKTLDVEGIFVELGYEARTEFVKHLVDLNENGEIITGKLGETRTPGLFAAGDVTDVPFKQ
CCEEEEEEEEEEECCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEECCCCCCCHHH
AIISAGDAAKAALAAFNYLQKRRGRNVTIRVTGRRLVGLSQKRR
HHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCC
>Mature Secondary Structure 
AEQTGQTVGRSVEGEVERHDVIIVGAGMAGLTAALYLARQGVDVLVVSRDLGGQLMQAT
CCCCCHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
IIENYPGIEAVKGPELAMRVEKQARTFGAKFRYGEQIVRIDKLEDGTFLLESNRGRRYQA
HHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCEEEE
EVVILAFGKTPKRMNIPGEDEYDGRGVSYCTICDGPLFRGRNTAVVGIGDQGLEAVALLA
EEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHH
GLCPKVYYVTPTKLFGEPDLIARIKSAPNVEILEEHEPVKVLGDGKRVTGLVVRNKKTGE
HCCCCEEEECCHHHCCCCHHHHHHCCCCCEEEEECCCCEEEEECCCEEEEEEEECCCCCC
EKTLDVEGIFVELGYEARTEFVKHLVDLNENGEIITGKLGETRTPGLFAAGDVTDVPFKQ
CCEEEEEEEEEEECCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEECCCCCCCHHH
AIISAGDAAKAALAAFNYLQKRRGRNVTIRVTGRRLVGLSQKRR
HHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: thioredoxin; NADP+

Specific reaction: thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]