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The map label for this gene is metG [H]

Identifier: 124027002

GI number: 124027002

Start: 91864

End: 92220

Strand: Direct

Name: metG [H]

Synonym: Hbut_0102

Alternate gene names: 124027002

Gene position: 91864-92220 (Clockwise)

Preceding gene: 124027001

Following gene: 124027003

Centisome position: 5.51

GC content: 54.34

Gene sequence:

>357_bases
GTGGCCTCCAATCAACCCATGGACAGAGAGAAGGAGTACATAACTATAGAGGAGTTTGCCCGCATCGACCTACGTGTTGG
CCGGGTTGTAGAGGCTGAGCGTATTCCTGGCACGAGGCTACTCCGCCTCATAGTGGATATTGGTACCGAGAAGAGGCAGA
TAATAGCTGGGCTCGGCGAGTTCTACAACCCCGAGGACTTTATAGGCAAGACAATGGTTATAGTTGCCAACCTCAAGCCT
AAGAGGATACGTGGTTACTTGAGTGAGGGCATGCTACTAGCTGCTGGCTGCGAGAAGGGACAGAAGCCGGTGCCCCTAAC
AGCTGAGGGAGCTGAGCCCGGCTGGAAGGTCTGCTAG

Upstream 100 bases:

>100_bases
GTGGCTGGCGAGAAGTTTGTCGAAATTCCACGCAGACCACAAGAATAAGGCTGTCCTAGATGACTAGGGTAGGCTCGGGT
TTTAGGAGGTGTGCGGGACC

Downstream 100 bases:

>100_bases
TATAATCTCCTTGGAGGGTCTGGCCGCAAAGATGGTTATATGCCTCGTGGGCTATGGCAGAATTGGGAGAATCACTGCAC
GCATACTCGCCGAAAAAGGG

Product: tRNA-binding protein - N-terminal MetRS

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 118; Mature: 117

Protein sequence:

>118_residues
MASNQPMDREKEYITIEEFARIDLRVGRVVEAERIPGTRLLRLIVDIGTEKRQIIAGLGEFYNPEDFIGKTMVIVANLKP
KRIRGYLSEGMLLAAGCEKGQKPVPLTAEGAEPGWKVC

Sequences:

>Translated_118_residues
MASNQPMDREKEYITIEEFARIDLRVGRVVEAERIPGTRLLRLIVDIGTEKRQIIAGLGEFYNPEDFIGKTMVIVANLKP
KRIRGYLSEGMLLAAGCEKGQKPVPLTAEGAEPGWKVC
>Mature_117_residues
ASNQPMDREKEYITIEEFARIDLRVGRVVEAERIPGTRLLRLIVDIGTEKRQIIAGLGEFYNPEDFIGKTMVIVANLKPK
RIRGYLSEGMLLAAGCEKGQKPVPLTAEGAEPGWKVC

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788432, Length=114, Percent_Identity=42.1052631578947, Blast_Score=86, Evalue=5e-19,
Organism=Escherichia coli, GI1789455, Length=104, Percent_Identity=36.5384615384615, Blast_Score=75, Evalue=1e-15,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 13173; Mature: 13041

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASNQPMDREKEYITIEEFARIDLRVGRVVEAERIPGTRLLRLIVDIGTEKRQIIAGLGE
CCCCCCCCCCCCEEEHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHH
FYNPEDFIGKTMVIVANLKPKRIRGYLSEGMLLAAGCEKGQKPVPLTAEGAEPGWKVC
CCCCHHHCCCEEEEEECCCHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
ASNQPMDREKEYITIEEFARIDLRVGRVVEAERIPGTRLLRLIVDIGTEKRQIIAGLGE
CCCCCCCCCCCEEEHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHH
FYNPEDFIGKTMVIVANLKPKRIRGYLSEGMLLAAGCEKGQKPVPLTAEGAEPGWKVC
CCCCHHHCCCEEEEEECCCHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9380682; 10360571 [H]