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The map label for this gene is aspS [H]

Identifier: 124026937

GI number: 124026937

Start: 26079

End: 27407

Strand: Direct

Name: aspS [H]

Synonym: Hbut_0034

Alternate gene names: 124026937

Gene position: 26079-27407 (Clockwise)

Preceding gene: 124026936

Following gene: 124026938

Centisome position: 1.56

GC content: 52.67

Gene sequence:

>1329_bases
TTGCCGCTAAAACTCCGAGAGCGTGTATATATACGTGACCTTCTCGAGAAAGGTGTCATAGGTAACGAGTATACGGTAGC
TGGCTGGGTAGACACAGTAAGAGTTCACGGCGGCCTAGTTTTCGTAGTTGTTAGAGATCGGACCGGCAAGATGCAGCTAG
TAGTTAAGCGTAACATATCGCGTGAAGCATGGGAGCATGCCCGTAAACTTGCGCCCGAGAGTGTGGTAGCGGCACGAGGC
AGGCTAGTGGAGAGTAAGGCTGCTCTCGGTGGCCGCGAGTTACAAGTCTACGAACTCGAGGTCCTCAACAAGGCTGACCC
CTTACCGATCGATATCTATGCGCCGGATAAGACAACACTTGCCAAGAGGCTTGACTGGCGCTTCCTAGACCTGCGCAATC
CACGCAACCAGCTCATAATGAGAATTGCGGCTGAGGTAGCTAGGGCCGCCAGGGAGTGGTTCGTCGAGAACGGCTTCATA
GAGATCTTCACGCCGAAGATTGTTGGTGCAGCCACTGAGGGAGGTGCTGAGGTCTTTAGCATAGTCTACTTCGACAAGCC
GGCATTTCTAGCACAGTCGCCGCAGCTTTACAAGCAGATGGGTGTTATAGCTGGCCTGGAGAAGGTGTTTGAGATAGGTC
CGGCATTCCGGGCAGAGCCCCACCACACGACTAGACACCTCACAGAGTATACGAGTATAGACCTTGAAATGGGCTTCATA
GACAGCTATGAAGATGTTATGGATATTGTTGAGGCGGTAATAAGACATGTGATCAGCAGCGTACTTTCGCGCTACCGGAG
CGAGATAAAAGAATACTTCCCTAATGCCATAACTGAGCCCCCCAAGGAGATACCGCGGATAACGATAAGGGAGGCCTACA
AGCTCCTAGAGGCAGCTGGTACTCCTGTGGAGTGGGGCGAGGACCTATCAAGTGAGGCCGAGAGAAAGCTCGGAGAGATA
GTTGAAAGGGAGTATGGCTCCTACCTCGTATTCGTGACAGAATACCCCTGGGCGGTTAGGCCATTCTACACGATGCGTAA
GAGCGATGAGCCGGATTGGACCTATAGCTTTGACCTCCTCATGAGAGGCCTAGAGATAGCTACTGGTGGCCAGAGGGAAC
ATCGCTACGACGTACTAGTGAAACAACTCGAGGAGAAGGGGCTAAACCCAAGAAACTTCGAATGGTACCTGGCAATGTTC
CGGTATGGTGCACCACCACACGGTGGTGCTGGCATAGGCTTAGAACGTGTCGTAATGCAGCTTCTAGGCTTAGGCAACAT
CCGTGAGGCACGGATGTTGCCAAGGGATCCGGAGAGGCTGGTGCCATAA

Upstream 100 bases:

>100_bases
TAGGTAAGACGTAGGGTTGGCTTGAGTCTAAACTACTGGCTTGAGAGCAATACCTATTACTATCCCTACTCTGCCTAGGC
AGCCCATTTGGGGCTGATGT

Downstream 100 bases:

>100_bases
CCAAGGAGGGCCCTTTTGATATTTAGGAGTTTGGAAATTGTGTATCAGCCCCCAGCGCCTCTTCATCCATCCCATGGGGT
GTCTGCGTCGTCTAGCATCA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MPLKLRERVYIRDLLEKGVIGNEYTVAGWVDTVRVHGGLVFVVVRDRTGKMQLVVKRNISREAWEHARKLAPESVVAARG
RLVESKAALGGRELQVYELEVLNKADPLPIDIYAPDKTTLAKRLDWRFLDLRNPRNQLIMRIAAEVARAAREWFVENGFI
EIFTPKIVGAATEGGAEVFSIVYFDKPAFLAQSPQLYKQMGVIAGLEKVFEIGPAFRAEPHHTTRHLTEYTSIDLEMGFI
DSYEDVMDIVEAVIRHVISSVLSRYRSEIKEYFPNAITEPPKEIPRITIREAYKLLEAAGTPVEWGEDLSSEAERKLGEI
VEREYGSYLVFVTEYPWAVRPFYTMRKSDEPDWTYSFDLLMRGLEIATGGQREHRYDVLVKQLEEKGLNPRNFEWYLAMF
RYGAPPHGGAGIGLERVVMQLLGLGNIREARMLPRDPERLVP

Sequences:

>Translated_442_residues
MPLKLRERVYIRDLLEKGVIGNEYTVAGWVDTVRVHGGLVFVVVRDRTGKMQLVVKRNISREAWEHARKLAPESVVAARG
RLVESKAALGGRELQVYELEVLNKADPLPIDIYAPDKTTLAKRLDWRFLDLRNPRNQLIMRIAAEVARAAREWFVENGFI
EIFTPKIVGAATEGGAEVFSIVYFDKPAFLAQSPQLYKQMGVIAGLEKVFEIGPAFRAEPHHTTRHLTEYTSIDLEMGFI
DSYEDVMDIVEAVIRHVISSVLSRYRSEIKEYFPNAITEPPKEIPRITIREAYKLLEAAGTPVEWGEDLSSEAERKLGEI
VEREYGSYLVFVTEYPWAVRPFYTMRKSDEPDWTYSFDLLMRGLEIATGGQREHRYDVLVKQLEEKGLNPRNFEWYLAMF
RYGAPPHGGAGIGLERVVMQLLGLGNIREARMLPRDPERLVP
>Mature_441_residues
PLKLRERVYIRDLLEKGVIGNEYTVAGWVDTVRVHGGLVFVVVRDRTGKMQLVVKRNISREAWEHARKLAPESVVAARGR
LVESKAALGGRELQVYELEVLNKADPLPIDIYAPDKTTLAKRLDWRFLDLRNPRNQLIMRIAAEVARAAREWFVENGFIE
IFTPKIVGAATEGGAEVFSIVYFDKPAFLAQSPQLYKQMGVIAGLEKVFEIGPAFRAEPHHTTRHLTEYTSIDLEMGFID
SYEDVMDIVEAVIRHVISSVLSRYRSEIKEYFPNAITEPPKEIPRITIREAYKLLEAAGTPVEWGEDLSSEAERKLGEIV
EREYGSYLVFVTEYPWAVRPFYTMRKSDEPDWTYSFDLLMRGLEIATGGQREHRYDVLVKQLEEKGLNPRNFEWYLAMFR
YGAPPHGGAGIGLERVVMQLLGLGNIREARMLPRDPERLVP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=440, Percent_Identity=37.5, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI226491502, Length=465, Percent_Identity=30.3225806451613, Blast_Score=195, Evalue=9e-50,
Organism=Homo sapiens, GI4758762, Length=439, Percent_Identity=30.2961275626424, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI40789249, Length=253, Percent_Identity=30.8300395256917, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI5031815, Length=460, Percent_Identity=25.6521739130435, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI194272210, Length=460, Percent_Identity=25.6521739130435, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1787161, Length=477, Percent_Identity=30.8176100628931, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1790571, Length=472, Percent_Identity=28.1779661016949, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1789256, Length=453, Percent_Identity=28.2560706401766, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1788173, Length=247, Percent_Identity=31.1740890688259, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17551876, Length=450, Percent_Identity=36.4444444444444, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71984122, Length=430, Percent_Identity=31.6279069767442, Blast_Score=207, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI17556372, Length=412, Percent_Identity=30.8252427184466, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI32566633, Length=262, Percent_Identity=31.6793893129771, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71994340, Length=462, Percent_Identity=25.974025974026, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17535927, Length=357, Percent_Identity=27.7310924369748, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=357, Percent_Identity=27.7310924369748, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323011, Length=467, Percent_Identity=36.6167023554604, Blast_Score=288, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6321807, Length=458, Percent_Identity=30.3493449781659, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6319872, Length=455, Percent_Identity=25.4945054945055, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6325153, Length=261, Percent_Identity=29.8850574712644, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17136276, Length=444, Percent_Identity=38.2882882882883, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI19921528, Length=429, Percent_Identity=31.7016317016317, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI19922416, Length=463, Percent_Identity=25.4859611231102, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24640849, Length=461, Percent_Identity=26.0303687635575, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24640851, Length=461, Percent_Identity=26.0303687635575, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24584738, Length=268, Percent_Identity=28.7313432835821, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 50582; Mature: 50451

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKLRERVYIRDLLEKGVIGNEYTVAGWVDTVRVHGGLVFVVVRDRTGKMQLVVKRNIS
CCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHEEECCCEEEEEEECCCCCEEEEEECCCC
REAWEHARKLAPESVVAARGRLVESKAALGGRELQVYELEVLNKADPLPIDIYAPDKTTL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEECCCHHHH
AKRLDWRFLDLRNPRNQLIMRIAAEVARAAREWFVENGFIEIFTPKIVGAATEGGAEVFS
HHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHCCCCCCCCCEEEE
IVYFDKPAFLAQSPQLYKQMGVIAGLEKVFEIGPAFRAEPHHTTRHLTEYTSIDLEMGFI
EEEECCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEEEECCC
DSYEDVMDIVEAVIRHVISSVLSRYRSEIKEYFPNAITEPPKEIPRITIREAYKLLEAAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHCC
TPVEWGEDLSSEAERKLGEIVEREYGSYLVFVTEYPWAVRPFYTMRKSDEPDWTYSFDLL
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHCCCCCCCCEEEHHHH
MRGLEIATGGQREHRYDVLVKQLEEKGLNPRNFEWYLAMFRYGAPPHGGAGIGLERVVMQ
HCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LLGLGNIREARMLPRDPERLVP
HHCCCCCHHHHCCCCCHHHCCC
>Mature Secondary Structure 
PLKLRERVYIRDLLEKGVIGNEYTVAGWVDTVRVHGGLVFVVVRDRTGKMQLVVKRNIS
CCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHEEECCCEEEEEEECCCCCEEEEEECCCC
REAWEHARKLAPESVVAARGRLVESKAALGGRELQVYELEVLNKADPLPIDIYAPDKTTL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEECCCHHHH
AKRLDWRFLDLRNPRNQLIMRIAAEVARAAREWFVENGFIEIFTPKIVGAATEGGAEVFS
HHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHCCCCCCCCCEEEE
IVYFDKPAFLAQSPQLYKQMGVIAGLEKVFEIGPAFRAEPHHTTRHLTEYTSIDLEMGFI
EEEECCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEEEECCC
DSYEDVMDIVEAVIRHVISSVLSRYRSEIKEYFPNAITEPPKEIPRITIREAYKLLEAAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHCC
TPVEWGEDLSSEAERKLGEIVEREYGSYLVFVTEYPWAVRPFYTMRKSDEPDWTYSFDLL
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHCCCCCCCCEEEHHHH
MRGLEIATGGQREHRYDVLVKQLEEKGLNPRNFEWYLAMFRYGAPPHGGAGIGLERVVMQ
HCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LLGLGNIREARMLPRDPERLVP
HHCCCCCHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]