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The map label for this gene is argG

Identifier: 124026895

GI number: 124026895

Start: 1850368

End: 1851570

Strand: Reverse

Name: argG

Synonym: NATL1_21901

Alternate gene names: 124026895

Gene position: 1851570-1850368 (Counterclockwise)

Preceding gene: 124026896

Following gene: 124026891

Centisome position: 99.29

GC content: 38.4

Gene sequence:

>1203_bases
ATGGGAAAGGCTAAAAAAGTTGTTTTGGCTTATTCAGGAGGGGTTGATACTAGCGTTTGTATTCCTTACTTGAAGAAGGA
ATATGGAGTCGAGCATGTAATTGCATTTGCAGCAGATCTTGGTCAAGGCGACGAGCTTGATCAAATCAAACAAAAGGCTA
TTTCAGCAGGAGCTTCAGAATCTTTAATTGGCAATTTGGTAAAGCCTTTCATAGAAGATTTTGCTTTCCCAGCGATTAGA
TCTAATGCTTTGTATCAAGGTAGATATCCTCTTTCAACGGCATTAGCTAGACCATTAATTGCAAAAAACCTTGTTGAAAT
TGCAAGGGAACTAAATGCCGATGGAGTCGCTCACGGGTGTACGGGCAAAGGGAACGATCAAGTGCGTTTTGATGTGACTA
TTGGAGCTTTAGCTCCTGATTTGCAATTGCTTACACCAGCACGGGAATGGGGTATGAGCCGCGAAGAAACCATTGCCTAT
GGAGAAAAATATGGAATAGTTCCCCCTGTAAGTAAAAAAACTCCTTACTCGATTGATTTGAATCTTTTGGGGAGAAGTAT
TGAAGCTGGTCCTCTTGAAGATCCATTTGAGATGCCATCAGAAGAAGTGTTTGGCATCACTTCTTCTATAGCTGATTCAC
CAAACGAGCCTGAGATAGTAGATATTTTGTTTGAAAATGGTTATCCAGTTGCAATTAATGGAGAAGCGATGGAGCCAGTA
TCCCTGATTAAAAAAGCTAATAGCCTTGCAGGAAAGCATGGCTTTGGACGTTTGGATATTATTGAAGACAGAGTAGTAGG
AATTAAAAGTCGAGAAATTTATGAAACTCCAGGATTGCTTTTATTAATTAAAGCTCATCAGGAAATTGAGAGTTTAACTT
TACCTGCCGACTTATTAGATACTAAATTTAGACTCGAACGACAATGGGCAGACTTGGTTTATAAAGGTTTTTGGTTTAGT
CCTCTAAAAGAAGCTTTGGATGGATTTATTAATTATTCTCAAAAGCAAGTGAATGGAACAGTCAGGGTTAGGCTTTTTAA
GGGTAATGTCGATGTTATAGGTCGCAAGTCAAAAGAAAATAGTTTGTATATTTCAGATATGTCTACTTATGGAAGTGAGG
ATAAGTTCAATCACAAATCCGCTGAAGGATTTATATATGTATGGGGATTGCCTAGTCGAATTTGGTCTTGGATAAACAAG
TAA

Upstream 100 bases:

>100_bases
AAATATTTGAATCAGGATTGATTATCGAAATCAGAAATATCGTCCAATAGTTCTCTATATTTACTAAGGAGAATTGAATT
TCCTTAAAGGAGAGAACCAT

Downstream 100 bases:

>100_bases
AATAAAGAATTTGTAATTTATTCCTTAGAGTCTTCTTTCTTCTCCTCTTGTGCAGGTGATTCAGTTTTGTCTTCAGTAGG
TTTTACTTCTTCCTTTTCTG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MGKAKKVVLAYSGGVDTSVCIPYLKKEYGVEHVIAFAADLGQGDELDQIKQKAISAGASESLIGNLVKPFIEDFAFPAIR
SNALYQGRYPLSTALARPLIAKNLVEIARELNADGVAHGCTGKGNDQVRFDVTIGALAPDLQLLTPAREWGMSREETIAY
GEKYGIVPPVSKKTPYSIDLNLLGRSIEAGPLEDPFEMPSEEVFGITSSIADSPNEPEIVDILFENGYPVAINGEAMEPV
SLIKKANSLAGKHGFGRLDIIEDRVVGIKSREIYETPGLLLLIKAHQEIESLTLPADLLDTKFRLERQWADLVYKGFWFS
PLKEALDGFINYSQKQVNGTVRVRLFKGNVDVIGRKSKENSLYISDMSTYGSEDKFNHKSAEGFIYVWGLPSRIWSWINK

Sequences:

>Translated_400_residues
MGKAKKVVLAYSGGVDTSVCIPYLKKEYGVEHVIAFAADLGQGDELDQIKQKAISAGASESLIGNLVKPFIEDFAFPAIR
SNALYQGRYPLSTALARPLIAKNLVEIARELNADGVAHGCTGKGNDQVRFDVTIGALAPDLQLLTPAREWGMSREETIAY
GEKYGIVPPVSKKTPYSIDLNLLGRSIEAGPLEDPFEMPSEEVFGITSSIADSPNEPEIVDILFENGYPVAINGEAMEPV
SLIKKANSLAGKHGFGRLDIIEDRVVGIKSREIYETPGLLLLIKAHQEIESLTLPADLLDTKFRLERQWADLVYKGFWFS
PLKEALDGFINYSQKQVNGTVRVRLFKGNVDVIGRKSKENSLYISDMSTYGSEDKFNHKSAEGFIYVWGLPSRIWSWINK
>Mature_399_residues
GKAKKVVLAYSGGVDTSVCIPYLKKEYGVEHVIAFAADLGQGDELDQIKQKAISAGASESLIGNLVKPFIEDFAFPAIRS
NALYQGRYPLSTALARPLIAKNLVEIARELNADGVAHGCTGKGNDQVRFDVTIGALAPDLQLLTPAREWGMSREETIAYG
EKYGIVPPVSKKTPYSIDLNLLGRSIEAGPLEDPFEMPSEEVFGITSSIADSPNEPEIVDILFENGYPVAINGEAMEPVS
LIKKANSLAGKHGFGRLDIIEDRVVGIKSREIYETPGLLLLIKAHQEIESLTLPADLLDTKFRLERQWADLVYKGFWFSP
LKEALDGFINYSQKQVNGTVRVRLFKGNVDVIGRKSKENSLYISDMSTYGSEDKFNHKSAEGFIYVWGLPSRIWSWINK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=403, Percent_Identity=40.4466501240695, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI16950633, Length=403, Percent_Identity=40.4466501240695, Blast_Score=274, Evalue=1e-73,
Organism=Escherichia coli, GI1789563, Length=377, Percent_Identity=29.973474801061, Blast_Score=130, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324514, Length=401, Percent_Identity=38.9027431421446, Blast_Score=265, Evalue=8e-72,
Organism=Drosophila melanogaster, GI21358151, Length=395, Percent_Identity=37.7215189873418, Blast_Score=261, Evalue=6e-70,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_PROM1 (A2C5I6)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001016010.1
- ProteinModelPortal:   A2C5I6
- SMR:   A2C5I6
- STRING:   A2C5I6
- GeneID:   4779237
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_21901
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- BioCyc:   PMAR167555:NATL1_21901-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44134; Mature: 44003

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 38-38 BINDING 89-89 BINDING 119-119 BINDING 121-121 BINDING 125-125 BINDING 125-125 BINDING 126-126 BINDING 129-129 BINDING 177-177 BINDING 186-186 BINDING 262-262 BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKAKKVVLAYSGGVDTSVCIPYLKKEYGVEHVIAFAADLGQGDELDQIKQKAISAGASE
CCCCEEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
SLIGNLVKPFIEDFAFPAIRSNALYQGRYPLSTALARPLIAKNLVEIARELNADGVAHGC
HHHHHHHHHHHHHHCCHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECC
TGKGNDQVRFDVTIGALAPDLQLLTPAREWGMSREETIAYGEKYGIVPPVSKKTPYSIDL
CCCCCCEEEEEEEECCCCCCHHHCCCHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCEEEH
NLLGRSIEAGPLEDPFEMPSEEVFGITSSIADSPNEPEIVDILFENGYPVAINGEAMEPV
EECCCCCCCCCCCCHHHCCCHHHHCCHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCHH
SLIKKANSLAGKHGFGRLDIIEDRVVGIKSREIYETPGLLLLIKAHQEIESLTLPADLLD
HHHHHHHHHHCCCCCCCEEEECCHHCCCCCCEEECCCCEEEEEECHHHHHHHCCCHHHHH
TKFRLERQWADLVYKGFWFSPLKEALDGFINYSQKQVNGTVRVRLFKGNVDVIGRKSKEN
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCCCEEEEEEEECCEEEEECCCCCC
SLYISDMSTYGSEDKFNHKSAEGFIYVWGLPSRIWSWINK
CEEEEEHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHCC
>Mature Secondary Structure 
GKAKKVVLAYSGGVDTSVCIPYLKKEYGVEHVIAFAADLGQGDELDQIKQKAISAGASE
CCCEEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
SLIGNLVKPFIEDFAFPAIRSNALYQGRYPLSTALARPLIAKNLVEIARELNADGVAHGC
HHHHHHHHHHHHHHCCHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECC
TGKGNDQVRFDVTIGALAPDLQLLTPAREWGMSREETIAYGEKYGIVPPVSKKTPYSIDL
CCCCCCEEEEEEEECCCCCCHHHCCCHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCEEEH
NLLGRSIEAGPLEDPFEMPSEEVFGITSSIADSPNEPEIVDILFENGYPVAINGEAMEPV
EECCCCCCCCCCCCHHHCCCHHHHCCHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCHH
SLIKKANSLAGKHGFGRLDIIEDRVVGIKSREIYETPGLLLLIKAHQEIESLTLPADLLD
HHHHHHHHHHCCCCCCCEEEECCHHCCCCCCEEECCCCEEEEEECHHHHHHHCCCHHHHH
TKFRLERQWADLVYKGFWFSPLKEALDGFINYSQKQVNGTVRVRLFKGNVDVIGRKSKEN
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCCCEEEEEEEECCEEEEECCCCCC
SLYISDMSTYGSEDKFNHKSAEGFIYVWGLPSRIWSWINK
CEEEEEHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA