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Identifier: 124026855

GI number: 124026855

Start: 1807011

End: 1809083

Strand: Direct

Name: lig

Synonym: NATL1_21501

Alternate gene names: 124026855

Gene position: 1807011-1809083 (Clockwise)

Preceding gene: 124026854

Following gene: 124026856

Centisome position: 96.9

GC content: 31.26

Gene sequence:

>2073_bases
GTGAATTCAAACTTTAATCAACATTTTGAACGTATAAAAGAGCTTAGAGCTTTACTCAATAAAGCAAATTACTCCTACTA
TGTATTAGATTCACCAGAGATAGACGATGCTGTTTATGATCAACTCTATCGAGAATTAATTGAAATTGAAAATATTCACC
CATCTTTAATTACTGATGATAGCCCCTCACAAAGATTAGGAGGTGTTCCTTCTAAAGGATTCAAAAATGTTGAGCATAAT
ATTCCTCTTTTAAGTCTAGATAATGCTTTTAATTTAAACGAATTAGAATCATGGTACGGAAGGATCAGCAAATTAATAAG
TTCAGAAAATAAAAATATCAAAAAAGTTGATGATCTAGAACTTATATGTGAATTAAAAATTGATGGAAATGCTATTTCAT
TAAGATATGAAAATGGAATTTTAACTAGAGCAGCAACTCGTGGAGATGGAAAAACTGGAGAAGATATCACTACTAATATC
AGAACAATTTCGACAATTCCTTTACGTTTATTATTAGAAAATCCACCCTCATGGGTTGAAATTAGAGGCGAGGCTTTCAT
GCCCAATAATATATTTAATAAACTCAATATTGAAAGAAAAAATACTGATCAACCACTCTTTGCAAATCCTAGAAACTCTT
GTGCTGGAACATTAAGACAATTAGATCCCAAAATAGTTGCATCTAGAAAACTTGACTTCTTCGCATACAGTCTTTATTTT
CCAGAAAATTGGGAGCCAACTGATAACAATTTTAAAAAACCAATTTCTCAATCTGAATCGCTTGAGTTTTTAAAAAATAT
TGGGTTTAAAGTTAATACTACATATGAAACAACAAAAACATTAAATGAAGCAAATAAGTATTACAAATATTGGGAAGTTA
AAAAAGATTTTTTAGCTTATGCAACTGATGGCATAGTAGTAAAAATAGATAAATTTGACATACAAAATCTATTAGGAGCA
ACCAATAAAGCTCCAAGATGGGCAATTGCTGTCAAATATCCAGCGGAAGAGAAAGCAACCAAGTTAAGAAAGATAATTTT
CCAAGTAGGTCGCTCTGGTGCAGTTACACCAGTAGCAGAATTTGAATCGATAGAGCTTGCAGGAACATCTGTGAATCGTG
CAACTCTTCATAATGCAAAAAGACTTGCCTCTTTAGATCTTCATTATGAAGACACAATAATTGTTCGAAAAGCCGGGGAG
ATTATTCCTGAAGTGATTAGGGTTATAAAAGAGTTTAGAAAAGTTGATGCAAAATTAGTTCAACTTCCTCAAAACTGCCC
AGAATGTAATTCGAAGTTAATTCTAGAATCAAACGAAGCAATTACAAAATGTATTAATTATGGATGCGAAGCAAAATTAA
AAGGTCTTTTACGTCATTGGGTCAGTAAAGGTTCAATGAACATAGATGGATTAGGTGAAAAAATTATTAATCAATTAGTA
AATGAAGGATATGTAAAGTCAATCGCTGATTTATACAAACTTGAAATTGATTCCCTTTTAGAGCTTGAAAGGTTTGGTGA
AAAATCTGCAAATAATTTACTAATAGAAATTAACGAATCTAAAAACAAAAATTGGCACAAACAACTGTACGGCTTAGGGA
TACCTCACATAGGAGAAGCGAATGCTAAATCTCTTTCGAACAACTTTAATAGTATCGAAGAACTTAATGCTATTGCGAAG
GAGTCACCTGAAAAAATATCCAATATTTATGGATTTGGGAATGAGATGAAAGATTCAATAGTTAAGTGGTTTGATGATTC
TAATAATCAAACCTTAATTAAAGAATTAAAAGCAATTGGATTTTCTCTAAAAGAGAGTTTAGATTCAAACTACAATTCAA
ATCAATCAAATGTTTTTGATGGTAAGAGTTTTGTCTTAACAGGGACTTTGGATTCACTTACAAGAGATGAAGCAAAAGAA
TTGATAGAAAGTGCTGGAGGGAAAGTCAGCTCTTCAATTAGTAAAAAAACAGACTTCTTAGTATCAGGAGAAAAAGCTGG
AAGTAAATTAAACAAAGCACAAGAGCTTGGAGTAAAAATAATTAACGAAAATGAATTGAAGCTATTACTATAA

Upstream 100 bases:

>100_bases
AAATGAAATGCCGCTGGATTTCACTTTTATTAAAATCAACGAAAATTGGCAAATTGATCGTATTGCTAAAGCCAATACAT
AATTAATCAGAATGTGATAA

Downstream 100 bases:

>100_bases
AATGGTCATAATTTAAAAATGCTTAAAAATAAAATTTTTTCTTTAATCAAAACTGAATGTGGGCGCGCTAAATATGAAAT
GCTGGCTTCCAAAAAAGGTA

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 690; Mature: 690

Protein sequence:

>690_residues
MNSNFNQHFERIKELRALLNKANYSYYVLDSPEIDDAVYDQLYRELIEIENIHPSLITDDSPSQRLGGVPSKGFKNVEHN
IPLLSLDNAFNLNELESWYGRISKLISSENKNIKKVDDLELICELKIDGNAISLRYENGILTRAATRGDGKTGEDITTNI
RTISTIPLRLLLENPPSWVEIRGEAFMPNNIFNKLNIERKNTDQPLFANPRNSCAGTLRQLDPKIVASRKLDFFAYSLYF
PENWEPTDNNFKKPISQSESLEFLKNIGFKVNTTYETTKTLNEANKYYKYWEVKKDFLAYATDGIVVKIDKFDIQNLLGA
TNKAPRWAIAVKYPAEEKATKLRKIIFQVGRSGAVTPVAEFESIELAGTSVNRATLHNAKRLASLDLHYEDTIIVRKAGE
IIPEVIRVIKEFRKVDAKLVQLPQNCPECNSKLILESNEAITKCINYGCEAKLKGLLRHWVSKGSMNIDGLGEKIINQLV
NEGYVKSIADLYKLEIDSLLELERFGEKSANNLLIEINESKNKNWHKQLYGLGIPHIGEANAKSLSNNFNSIEELNAIAK
ESPEKISNIYGFGNEMKDSIVKWFDDSNNQTLIKELKAIGFSLKESLDSNYNSNQSNVFDGKSFVLTGTLDSLTRDEAKE
LIESAGGKVSSSISKKTDFLVSGEKAGSKLNKAQELGVKIINENELKLLL

Sequences:

>Translated_690_residues
MNSNFNQHFERIKELRALLNKANYSYYVLDSPEIDDAVYDQLYRELIEIENIHPSLITDDSPSQRLGGVPSKGFKNVEHN
IPLLSLDNAFNLNELESWYGRISKLISSENKNIKKVDDLELICELKIDGNAISLRYENGILTRAATRGDGKTGEDITTNI
RTISTIPLRLLLENPPSWVEIRGEAFMPNNIFNKLNIERKNTDQPLFANPRNSCAGTLRQLDPKIVASRKLDFFAYSLYF
PENWEPTDNNFKKPISQSESLEFLKNIGFKVNTTYETTKTLNEANKYYKYWEVKKDFLAYATDGIVVKIDKFDIQNLLGA
TNKAPRWAIAVKYPAEEKATKLRKIIFQVGRSGAVTPVAEFESIELAGTSVNRATLHNAKRLASLDLHYEDTIIVRKAGE
IIPEVIRVIKEFRKVDAKLVQLPQNCPECNSKLILESNEAITKCINYGCEAKLKGLLRHWVSKGSMNIDGLGEKIINQLV
NEGYVKSIADLYKLEIDSLLELERFGEKSANNLLIEINESKNKNWHKQLYGLGIPHIGEANAKSLSNNFNSIEELNAIAK
ESPEKISNIYGFGNEMKDSIVKWFDDSNNQTLIKELKAIGFSLKESLDSNYNSNQSNVFDGKSFVLTGTLDSLTRDEAKE
LIESAGGKVSSSISKKTDFLVSGEKAGSKLNKAQELGVKIINENELKLLL
>Mature_690_residues
MNSNFNQHFERIKELRALLNKANYSYYVLDSPEIDDAVYDQLYRELIEIENIHPSLITDDSPSQRLGGVPSKGFKNVEHN
IPLLSLDNAFNLNELESWYGRISKLISSENKNIKKVDDLELICELKIDGNAISLRYENGILTRAATRGDGKTGEDITTNI
RTISTIPLRLLLENPPSWVEIRGEAFMPNNIFNKLNIERKNTDQPLFANPRNSCAGTLRQLDPKIVASRKLDFFAYSLYF
PENWEPTDNNFKKPISQSESLEFLKNIGFKVNTTYETTKTLNEANKYYKYWEVKKDFLAYATDGIVVKIDKFDIQNLLGA
TNKAPRWAIAVKYPAEEKATKLRKIIFQVGRSGAVTPVAEFESIELAGTSVNRATLHNAKRLASLDLHYEDTIIVRKAGE
IIPEVIRVIKEFRKVDAKLVQLPQNCPECNSKLILESNEAITKCINYGCEAKLKGLLRHWVSKGSMNIDGLGEKIINQLV
NEGYVKSIADLYKLEIDSLLELERFGEKSANNLLIEINESKNKNWHKQLYGLGIPHIGEANAKSLSNNFNSIEELNAIAK
ESPEKISNIYGFGNEMKDSIVKWFDDSNNQTLIKELKAIGFSLKESLDSNYNSNQSNVFDGKSFVLTGTLDSLTRDEAKE
LIESAGGKVSSSISKKTDFLVSGEKAGSKLNKAQELGVKIINENELKLLL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=71, Percent_Identity=54.9295774647887, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1788750, Length=682, Percent_Identity=42.2287390029325, Blast_Score=550, Evalue=1e-157,
Organism=Escherichia coli, GI87082305, Length=595, Percent_Identity=22.8571428571429, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_PROM1 (A2C5E6)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015970.1
- ProteinModelPortal:   A2C5E6
- STRING:   A2C5E6
- GeneID:   4780722
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_21501
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK2516566
- BioCyc:   PMAR167555:NATL1_21501-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 77842; Mature: 77842

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 126-126 BINDING 124-124 BINDING 147-147 BINDING 184-184 BINDING 308-308 BINDING 332-332

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSNFNQHFERIKELRALLNKANYSYYVLDSPEIDDAVYDQLYRELIEIENIHPSLITDD
CCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
SPSQRLGGVPSKGFKNVEHNIPLLSLDNAFNLNELESWYGRISKLISSENKNIKKVDDLE
CCHHHHCCCCCCCCCCHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
LICELKIDGNAISLRYENGILTRAATRGDGKTGEDITTNIRTISTIPLRLLLENPPSWVE
EEEEEEECCCEEEEEECCCEEEEEECCCCCCCCCCHHCCCEEEEECCEEEEECCCCCEEE
IRGEAFMPNNIFNKLNIERKNTDQPLFANPRNSCAGTLRQLDPKIVASRKLDFFAYSLYF
EECCEECCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCEEEEEEEEEC
PENWEPTDNNFKKPISQSESLEFLKNIGFKVNTTYETTKTLNEANKYYKYWEVKKDFLAY
CCCCCCCCCCHHCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
ATDGIVVKIDKFDIQNLLGATNKAPRWAIAVKYPAEEKATKLRKIIFQVGRSGAVTPVAE
CCCCEEEEEECHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHH
FESIELAGTSVNRATLHNAKRLASLDLHYEDTIIVRKAGEIIPEVIRVIKEFRKVDAKLV
CCCEEEECCCCCHHHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
QLPQNCPECNSKLILESNEAITKCINYGCEAKLKGLLRHWVSKGSMNIDGLGEKIINQLV
HCCCCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
NEGYVKSIADLYKLEIDSLLELERFGEKSANNLLIEINESKNKNWHKQLYGLGIPHIGEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCC
NAKSLSNNFNSIEELNAIAKESPEKISNIYGFGNEMKDSIVKWFDDSNNQTLIKELKAIG
CHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
FSLKESLDSNYNSNQSNVFDGKSFVLTGTLDSLTRDEAKELIESAGGKVSSSISKKTDFL
HHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEE
VSGEKAGSKLNKAQELGVKIINENELKLLL
EECHHHHHHHHHHHHCCEEEECCCCEEEEC
>Mature Secondary Structure
MNSNFNQHFERIKELRALLNKANYSYYVLDSPEIDDAVYDQLYRELIEIENIHPSLITDD
CCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
SPSQRLGGVPSKGFKNVEHNIPLLSLDNAFNLNELESWYGRISKLISSENKNIKKVDDLE
CCHHHHCCCCCCCCCCHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
LICELKIDGNAISLRYENGILTRAATRGDGKTGEDITTNIRTISTIPLRLLLENPPSWVE
EEEEEEECCCEEEEEECCCEEEEEECCCCCCCCCCHHCCCEEEEECCEEEEECCCCCEEE
IRGEAFMPNNIFNKLNIERKNTDQPLFANPRNSCAGTLRQLDPKIVASRKLDFFAYSLYF
EECCEECCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCEEEEEEEEEC
PENWEPTDNNFKKPISQSESLEFLKNIGFKVNTTYETTKTLNEANKYYKYWEVKKDFLAY
CCCCCCCCCCHHCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
ATDGIVVKIDKFDIQNLLGATNKAPRWAIAVKYPAEEKATKLRKIIFQVGRSGAVTPVAE
CCCCEEEEEECHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHH
FESIELAGTSVNRATLHNAKRLASLDLHYEDTIIVRKAGEIIPEVIRVIKEFRKVDAKLV
CCCEEEECCCCCHHHHHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
QLPQNCPECNSKLILESNEAITKCINYGCEAKLKGLLRHWVSKGSMNIDGLGEKIINQLV
HCCCCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
NEGYVKSIADLYKLEIDSLLELERFGEKSANNLLIEINESKNKNWHKQLYGLGIPHIGEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCC
NAKSLSNNFNSIEELNAIAKESPEKISNIYGFGNEMKDSIVKWFDDSNNQTLIKELKAIG
CHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
FSLKESLDSNYNSNQSNVFDGKSFVLTGTLDSLTRDEAKELIESAGGKVSSSISKKTDFL
HHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEE
VSGEKAGSKLNKAQELGVKIINENELKLLL
EECHHHHHHHHHHHHCCEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA