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The map label for this gene is gcvH

Identifier: 124026844

GI number: 124026844

Start: 1797278

End: 1797664

Strand: Reverse

Name: gcvH

Synonym: NATL1_21391

Alternate gene names: 124026844

Gene position: 1797664-1797278 (Counterclockwise)

Preceding gene: 124026845

Following gene: 124026843

Centisome position: 96.4

GC content: 40.83

Gene sequence:

>387_bases
ATGGCTTTTTCCTTCCCAGATCATTTTCGTTTTGCTGACAGTCATGAATATGCTTTTGCAGATGATGCTTTAGTTCGAAT
TGGCATCAGTGAATTTGCAGTCGATCAGTTAGGAGACATTGTTTTCGTTGATCTTCCAGAGGAAGGCACTGCAATAGCCA
AAGGAGAGAGCTTTGGGTCAGTCGAATCAGTAAAGGCTGTTGAGGATATGTATGCTCCAGTGAGCGGAGAGATTGTTCAT
AGAAACAATTCTGTTTTAGCAAGCCCAGAGGAACTTCAAAATGATCCTCATGGCGAAGGATGGTTATTAATAATTCGCCC
TGATAACCCTGCTCAACTAACAGAACTTATGGACTCTGAAACTTATTCAAAGAAAATCTCTGCATAA

Upstream 100 bases:

>100_bases
CTCATGTCAAAATTGCTTTGATTCATGCATTATCCAATTTATTTCAGGCAGGATTGATCAAATTACCCCAGAATGATTAA
GCGTTTTTCGTTTTGACGCA

Downstream 100 bases:

>100_bases
AATCTTGCTGAATAATTTTTTCTTACAACAAATTGGGAAATTTTTCTCTACACTGGCTCTCTTTCCAATAACTTAATTTG
CTTGTGCTTGAGAACAAGCT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MAFSFPDHFRFADSHEYAFADDALVRIGISEFAVDQLGDIVFVDLPEEGTAIAKGESFGSVESVKAVEDMYAPVSGEIVH
RNNSVLASPEELQNDPHGEGWLLIIRPDNPAQLTELMDSETYSKKISA

Sequences:

>Translated_128_residues
MAFSFPDHFRFADSHEYAFADDALVRIGISEFAVDQLGDIVFVDLPEEGTAIAKGESFGSVESVKAVEDMYAPVSGEIVH
RNNSVLASPEELQNDPHGEGWLLIIRPDNPAQLTELMDSETYSKKISA
>Mature_127_residues
AFSFPDHFRFADSHEYAFADDALVRIGISEFAVDQLGDIVFVDLPEEGTAIAKGESFGSVESVKAVEDMYAPVSGEIVHR
NNSVLASPEELQNDPHGEGWLLIIRPDNPAQLTELMDSETYSKKISA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=37.8151260504202, Blast_Score=102, Evalue=1e-22,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=37.8151260504202, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=39.1666666666667, Blast_Score=98, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17551294, Length=124, Percent_Identity=41.1290322580645, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17507493, Length=114, Percent_Identity=38.5964912280702, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=37.6, Blast_Score=93, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=34.1666666666667, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_PROM1 (A2C5D5)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015959.1
- ProteinModelPortal:   A2C5D5
- SMR:   A2C5D5
- STRING:   A2C5D5
- GeneID:   4780855
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_21391
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TPKELRY
- ProtClustDB:   PRK01202
- BioCyc:   PMAR167555:NATL1_21391-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14060; Mature: 13928

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSFPDHFRFADSHEYAFADDALVRIGISEFAVDQLGDIVFVDLPEEGTAIAKGESFGS
CCCCCCCCCCCCCCCCEEECCCCEEEECHHHHHHHHCCCEEEEECCCCCCEEECCCCCCC
VESVKAVEDMYAPVSGEIVHRNNSVLASPEELQNDPHGEGWLLIIRPDNPAQLTELMDSE
HHHHHHHHHHHCCCCCEEEECCCCEEECHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCH
TYSKKISA
HHHHCCCC
>Mature Secondary Structure 
AFSFPDHFRFADSHEYAFADDALVRIGISEFAVDQLGDIVFVDLPEEGTAIAKGESFGS
CCCCCCCCCCCCCCCEEECCCCEEEECHHHHHHHHCCCEEEEECCCCCCEEECCCCCCC
VESVKAVEDMYAPVSGEIVHRNNSVLASPEELQNDPHGEGWLLIIRPDNPAQLTELMDSE
HHHHHHHHHHHCCCCCEEEECCCCEEECHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCH
TYSKKISA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA