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The map label for this gene is gca [H]

Identifier: 124026811

GI number: 124026811

Start: 1757594

End: 1758523

Strand: Direct

Name: gca [H]

Synonym: NATL1_21061

Alternate gene names: 124026811

Gene position: 1757594-1758523 (Clockwise)

Preceding gene: 124026807

Following gene: 124026815

Centisome position: 94.25

GC content: 30.75

Gene sequence:

>930_bases
ATGGTTTTTGGCTGTACAGGGCAAGATGGATCTCTAATTTCTAATTCACTGCTAAGAAAAGGTTATGAAGTCTTAGGGAT
TACGAGAAGTAGCAAACCTAATTATCAATATCTTCAACAACTCGGTATTAACCAATCTTTTGAAATTAAAAAAATTGGTG
TTGAGGATGATCTTTTGTCTTTTCAGAAATTGATAGAATTTTACGATCCTATTGAGATTTATAATTTATCTGCTCAAAGC
TCAGTAGGATTATCATTTAAGGAACCATATAATTCAATTAAAAGTATTTACCATCAAACATTAATTCTGTTAGAAGCAAC
TAGAACAATCGAATTTAATGGCACTATTTTCCTCGCTGGCAGCAGTGAAATGTTTGGAGAGACAAAAGTTGCTGCTGATA
TCAATCATCCTAGAAAGCCATTAAGTCCATATGCTCACGCAAAAGAAGCATCCTTTAGTTTAGTTAAACAATATAGAAGA
ATTTATAATATTAATTGCGTAACAGGAGTCCTATTTAATCATGAATCTACGTTAAGGACTGATAGATTTGTAATTCCAAA
AATTATTAAAGGTGCCATTCAATGCAAAAAAAACAAAACCTATAAACTCAGTCTTGGAAATATTGATATTTATAGAGATT
GGGGTTGGGCAGAGGATTATGTAGAAGCTATGCAAAGGATTACAAGAGCAGATAAAAAGCAAGACCATGTAATTTGTACG
GGAAATTTAACATCATTAAAAGATTTTATAAAGAAAGTATTTGATAAAATGGATCTAAATTGGCAAGATCATGTCACAAT
AAATAAAGGAAATAAGAGACCTCACGATATTTTAAAAAGTTTTGGGAGTCCGCAAGCTTTAAAATTAGAATTAGATTGGG
AAAATAAAAAGAGTATTGAAGATATAATTGAATTACTTTTGGAAGGTTGA

Upstream 100 bases:

>100_bases
GTTATTTATCAGTAATTTTTAATTCAGTCTAAATAAGACACTTTTGAGATATATTGCATTTTAATTTATGTGAATTAATT
CCTTGACCAATAAACTTTGC

Downstream 100 bases:

>100_bases
TTGAAATAAAGGTTATTAGAGGAAATTTCTATCGACTTCTAATGCAGTAATTAATCGGCTTAACCTGCTTGATAAATTAC
TGAATAGATGACTTTTTCTC

Product: GDP-D-mannose dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MVFGCTGQDGSLISNSLLRKGYEVLGITRSSKPNYQYLQQLGINQSFEIKKIGVEDDLLSFQKLIEFYDPIEIYNLSAQS
SVGLSFKEPYNSIKSIYHQTLILLEATRTIEFNGTIFLAGSSEMFGETKVAADINHPRKPLSPYAHAKEASFSLVKQYRR
IYNINCVTGVLFNHESTLRTDRFVIPKIIKGAIQCKKNKTYKLSLGNIDIYRDWGWAEDYVEAMQRITRADKKQDHVICT
GNLTSLKDFIKKVFDKMDLNWQDHVTINKGNKRPHDILKSFGSPQALKLELDWENKKSIEDIIELLLEG

Sequences:

>Translated_309_residues
MVFGCTGQDGSLISNSLLRKGYEVLGITRSSKPNYQYLQQLGINQSFEIKKIGVEDDLLSFQKLIEFYDPIEIYNLSAQS
SVGLSFKEPYNSIKSIYHQTLILLEATRTIEFNGTIFLAGSSEMFGETKVAADINHPRKPLSPYAHAKEASFSLVKQYRR
IYNINCVTGVLFNHESTLRTDRFVIPKIIKGAIQCKKNKTYKLSLGNIDIYRDWGWAEDYVEAMQRITRADKKQDHVICT
GNLTSLKDFIKKVFDKMDLNWQDHVTINKGNKRPHDILKSFGSPQALKLELDWENKKSIEDIIELLLEG
>Mature_309_residues
MVFGCTGQDGSLISNSLLRKGYEVLGITRSSKPNYQYLQQLGINQSFEIKKIGVEDDLLSFQKLIEFYDPIEIYNLSAQS
SVGLSFKEPYNSIKSIYHQTLILLEATRTIEFNGTIFLAGSSEMFGETKVAADINHPRKPLSPYAHAKEASFSLVKQYRR
IYNINCVTGVLFNHESTLRTDRFVIPKIIKGAIQCKKNKTYKLSLGNIDIYRDWGWAEDYVEAMQRITRADKKQDHVICT
GNLTSLKDFIKKVFDKMDLNWQDHVTINKGNKRPHDILKSFGSPQALKLELDWENKKSIEDIIELLLEG

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=335, Percent_Identity=32.5373134328358, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI1788366, Length=260, Percent_Identity=33.8461538461538, Blast_Score=136, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI133901788, Length=334, Percent_Identity=32.0359281437126, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17539422, Length=334, Percent_Identity=32.0359281437126, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17539424, Length=334, Percent_Identity=32.0359281437126, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI133901786, Length=334, Percent_Identity=32.0359281437126, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI133901790, Length=334, Percent_Identity=32.0359281437126, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17507723, Length=334, Percent_Identity=32.3353293413174, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24158427, Length=335, Percent_Identity=32.8358208955224, Blast_Score=157, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 35372; Mature: 35372

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFGCTGQDGSLISNSLLRKGYEVLGITRSSKPNYQYLQQLGINQSFEIKKIGVEDDLLS
CEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEECCCCHHHHH
FQKLIEFYDPIEIYNLSAQSSVGLSFKEPYNSIKSIYHQTLILLEATRTIEFNGTIFLAG
HHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEEEECCEEEEEC
SSEMFGETKVAADINHPRKPLSPYAHAKEASFSLVKQYRRIYNINCVTGVLFNHESTLRT
CHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCHHCC
DRFVIPKIIKGAIQCKKNKTYKLSLGNIDIYRDWGWAEDYVEAMQRITRADKKQDHVICT
CCCHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEE
GNLTSLKDFIKKVFDKMDLNWQDHVTINKGNKRPHDILKSFGSPQALKLELDWENKKSIE
CCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCCHH
DIIELLLEG
HHHHHHHCC
>Mature Secondary Structure
MVFGCTGQDGSLISNSLLRKGYEVLGITRSSKPNYQYLQQLGINQSFEIKKIGVEDDLLS
CEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEECCCCHHHHH
FQKLIEFYDPIEIYNLSAQSSVGLSFKEPYNSIKSIYHQTLILLEATRTIEFNGTIFLAG
HHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEEEECCEEEEEC
SSEMFGETKVAADINHPRKPLSPYAHAKEASFSLVKQYRRIYNINCVTGVLFNHESTLRT
CHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCHHCC
DRFVIPKIIKGAIQCKKNKTYKLSLGNIDIYRDWGWAEDYVEAMQRITRADKKQDHVICT
CCCHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEE
GNLTSLKDFIKKVFDKMDLNWQDHVTINKGNKRPHDILKSFGSPQALKLELDWENKKSIE
CCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCCHH
DIIELLLEG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]