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The map label for this gene is gyrB [H]

Identifier: 124026793

GI number: 124026793

Start: 1729222

End: 1731189

Strand: Reverse

Name: gyrB [H]

Synonym: NATL1_20881

Alternate gene names: 124026793

Gene position: 1731189-1729222 (Counterclockwise)

Preceding gene: 124026798

Following gene: 124026792

Centisome position: 92.84

GC content: 36.84

Gene sequence:

>1968_bases
ATGAGTAAAGATTCAAAAGTCCAAGAGGCTTATGGTGCTGAGCAGATCCAAGTTCTTGAAGGCTTGGAACCTGTCAGGAA
GCGGCCTGGAATGTATATAGGCTCGACAGGGTCTAAAGGACTTCATCATCTTGTGTATGAAGTTGTTGATAATGCTGTAG
ACGAGGCACTGGCTGGTCACTGTGATCAAATCACAATATTGCTTTGTGAGGATGGTTCGGCATCAGTGTCAGACAATGGT
CGGGGAATTCCAACTGACTTACATCCTCGCACTGGTAAAAGTGCTCTTGAAACCGTTCTAACTGTTTTGCATGCTGGTGG
CAAATTTGGAAGCGGTGGTTATAAGGTTTCTGGAGGTTTGCATGGTGTTGGAATATCGGTTGTAAATGCTTTAAGTGAAT
GGGTGGAAGTAACAGTTAGGAGGCAACAACAAGTTCATTTTCAGCGGTTCGAAAGAGGCGCATCAATTGGGAGTCTAAAG
TCTGAAAATCTTTCGAAATCAGATAAAAATTTAACTGGTACTACTGTTTGCTTTAAACCTGATGATCAAATTTTTACGGA
TGGAATATCGTTTGAATACGGCATTTTATCATCGAGATTAAGAGAACTAGCCTATTTAAATGGAGGGGTTCGTATCGTTT
TTCGTGACGAGAGAAAACAATCGAATGATTCTTCACAAACTCCACATGAAGAAGTTTATTTTTATGAAGGTGGAATTAAA
GAATATGTGGAGTATATGACTAAAGCAAAAGACTCATTACATCCAGAGATTATTTATGTCAATTCAGAAAAAGATGGAGT
TCAAGTAGAAGCAGCAATGCAATGGTGCGTCGATGCCTATTCAGATAGCATTCTTGGGTTTGCTAATAATATTCGCACCA
TAGATGGTGGAACTCATATTGAGGGCTTGAAAACAGTTTTAACTAGAACGTTAAACTCATTTGCAAAGAAAAGAGGTAAA
CGAAAAGATGGAGATTCGAATTTAGCAGGAGAAAATATTCGAGAAGGTTTAACAGCTGTTTTATCTGTAAAAGTTCCAGA
TCCAGAATTTGAAGGTCAAACAAAAACTAAATTAGGTAATACTGAGGTACGAGGAATTGTTGATAGTTTGGTAGGTACGT
CTCTAAGTCAATATCTAGAATTCAACCCAAATGTTATTGACTTAATTTTAGAGAAAGCAATACAAGCATTTAATGCTGCA
GAGGCAGCAAGAAGAGCTAGAGAATTGGTCCGTAGAAAAAGTGTTTTAGAAAGTTCTACTTTGCCAGGTAAATTGGCTGA
CTGTAGCTCAAGAGACCCATCAGAATCAGAAATTTATATAGTTGAGGGAGATTCTGCTGGAGGATCGGCTAAGCAGGGCA
GAGATCGAAAATTTCAAGCAATCTTACCTTTGAGAGGAAAAATTCTAAATATTGAGAAAACCGATGATGCGAAGATGTAT
AAAAATACTGAGATTCAATCTTTAATAACTGCTCTTGGTTTAGGAATAAAAGGTGAGGATTTTGAAGTTAAGAATCTCAG
ATATCACAGAGTAGTAATCATGACTGATGCTGATGTAGATGGTGCTCATATAAGAACGTTACTTTTGACTTTCTTTTATA
GATATCAAAAGGATCTTGTAGAAGGTGGATATATTTATATTGCTTGTCCTCCTCTCTATAAAGTTACAAGAGGAAAGAAT
CATAAATATTGTTATAACGAATCAGATATGCAAAAAACTCTTGCAAGTTTTGGAGAAAAAGCCAACTATACAATCCAACG
ATTTAAAGGTTTAGGAGAAATGATGCCTAAACAACTATGGGATACAACTATGGACCCAACAACTAGAATGATGAAAAGAG
TTGAGATAGAAGATGCTCTTGAAGCTGATCGGATATTTACAATTTTAATGGGAGATAAAGTCGCACCAAGAAGAGAATTT
ATTGAAACTCATAGTGTTGATTTGGATCTAGCTACTTTAGATATTTGA

Upstream 100 bases:

>100_bases
CCTAAGACTTTCCTTGATACCAATGTTAAATTGAATCTTGGGGAAAGGTTTCGGCTAATGCTCGTATCAAATACAACCTT
GCCTGCATCCGTTTATCAGA

Downstream 100 bases:

>100_bases
TGAATTTAATTAGTACGCTTATTGTTTGGGCATGGCTTTTATGTATTGGTTTGGTAGCCCCTACTACATTACCTGCCGGG
GGTGCTCAAGAATCAATTAT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MSKDSKVQEAYGAEQIQVLEGLEPVRKRPGMYIGSTGSKGLHHLVYEVVDNAVDEALAGHCDQITILLCEDGSASVSDNG
RGIPTDLHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVEVTVRRQQQVHFQRFERGASIGSLK
SENLSKSDKNLTGTTVCFKPDDQIFTDGISFEYGILSSRLRELAYLNGGVRIVFRDERKQSNDSSQTPHEEVYFYEGGIK
EYVEYMTKAKDSLHPEIIYVNSEKDGVQVEAAMQWCVDAYSDSILGFANNIRTIDGGTHIEGLKTVLTRTLNSFAKKRGK
RKDGDSNLAGENIREGLTAVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLVGTSLSQYLEFNPNVIDLILEKAIQAFNAA
EAARRARELVRRKSVLESSTLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDAKMY
KNTEIQSLITALGLGIKGEDFEVKNLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQKDLVEGGYIYIACPPLYKVTRGKN
HKYCYNESDMQKTLASFGEKANYTIQRFKGLGEMMPKQLWDTTMDPTTRMMKRVEIEDALEADRIFTILMGDKVAPRREF
IETHSVDLDLATLDI

Sequences:

>Translated_655_residues
MSKDSKVQEAYGAEQIQVLEGLEPVRKRPGMYIGSTGSKGLHHLVYEVVDNAVDEALAGHCDQITILLCEDGSASVSDNG
RGIPTDLHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVEVTVRRQQQVHFQRFERGASIGSLK
SENLSKSDKNLTGTTVCFKPDDQIFTDGISFEYGILSSRLRELAYLNGGVRIVFRDERKQSNDSSQTPHEEVYFYEGGIK
EYVEYMTKAKDSLHPEIIYVNSEKDGVQVEAAMQWCVDAYSDSILGFANNIRTIDGGTHIEGLKTVLTRTLNSFAKKRGK
RKDGDSNLAGENIREGLTAVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLVGTSLSQYLEFNPNVIDLILEKAIQAFNAA
EAARRARELVRRKSVLESSTLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDAKMY
KNTEIQSLITALGLGIKGEDFEVKNLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQKDLVEGGYIYIACPPLYKVTRGKN
HKYCYNESDMQKTLASFGEKANYTIQRFKGLGEMMPKQLWDTTMDPTTRMMKRVEIEDALEADRIFTILMGDKVAPRREF
IETHSVDLDLATLDI
>Mature_654_residues
SKDSKVQEAYGAEQIQVLEGLEPVRKRPGMYIGSTGSKGLHHLVYEVVDNAVDEALAGHCDQITILLCEDGSASVSDNGR
GIPTDLHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVEVTVRRQQQVHFQRFERGASIGSLKS
ENLSKSDKNLTGTTVCFKPDDQIFTDGISFEYGILSSRLRELAYLNGGVRIVFRDERKQSNDSSQTPHEEVYFYEGGIKE
YVEYMTKAKDSLHPEIIYVNSEKDGVQVEAAMQWCVDAYSDSILGFANNIRTIDGGTHIEGLKTVLTRTLNSFAKKRGKR
KDGDSNLAGENIREGLTAVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLVGTSLSQYLEFNPNVIDLILEKAIQAFNAAE
AARRARELVRRKSVLESSTLPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDAKMYK
NTEIQSLITALGLGIKGEDFEVKNLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQKDLVEGGYIYIACPPLYKVTRGKNH
KYCYNESDMQKTLASFGEKANYTIQRFKGLGEMMPKQLWDTTMDPTTRMMKRVEIEDALEADRIFTILMGDKVAPRREFI
ETHSVDLDLATLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=613, Percent_Identity=23.3278955954323, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI19913408, Length=627, Percent_Identity=23.2854864433812, Blast_Score=114, Evalue=4e-25,
Organism=Escherichia coli, GI48994957, Length=610, Percent_Identity=50.4918032786885, Blast_Score=576, Evalue=1e-165,
Organism=Escherichia coli, GI1789408, Length=652, Percent_Identity=39.2638036809816, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI212645845, Length=559, Percent_Identity=25.7602862254025, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17535065, Length=594, Percent_Identity=22.7272727272727, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=181, Percent_Identity=28.1767955801105, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=642, Percent_Identity=24.7663551401869, Blast_Score=120, Evalue=6e-28,
Organism=Drosophila melanogaster, GI17136538, Length=580, Percent_Identity=24.1379310344828, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72707; Mature: 72576

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDSKVQEAYGAEQIQVLEGLEPVRKRPGMYIGSTGSKGLHHLVYEVVDNAVDEALAGH
CCCCHHHHHHHCHHHHHHHHCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
CDQITILLCEDGSASVSDNGRGIPTDLHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGL
CCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGISVVNALSEWVEVTVRRQQQVHFQRFERGASIGSLKSENLSKSDKNLTGTTVCFKP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECC
DDQIFTDGISFEYGILSSRLRELAYLNGGVRIVFRDERKQSNDSSQTPHEEVYFYEGGIK
CCCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCHH
EYVEYMTKAKDSLHPEIIYVNSEKDGVQVEAAMQWCVDAYSDSILGFANNIRTIDGGTHI
HHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCH
EGLKTVLTRTLNSFAKKRGKRKDGDSNLAGENIREGLTAVLSVKVPDPEFEGQTKTKLGN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCEEEEEEECCCCCCCCCCHHCCCC
TEVRGIVDSLVGTSLSQYLEFNPNVIDLILEKAIQAFNAAEAARRARELVRRKSVLESST
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDAKMY
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCHHH
KNTEIQSLITALGLGIKGEDFEVKNLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQKDLV
CCCHHHHHHHHHCCCCCCCCCEECCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
EGGYIYIACPPLYKVTRGKNHKYCYNESDMQKTLASFGEKANYTIQRFKGLGEMMPKQLW
CCCEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
DTTMDPTTRMMKRVEIEDALEADRIFTILMGDKVAPRREFIETHSVDLDLATLDI
CCCCCHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
SKDSKVQEAYGAEQIQVLEGLEPVRKRPGMYIGSTGSKGLHHLVYEVVDNAVDEALAGH
CCCHHHHHHHCHHHHHHHHCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
CDQITILLCEDGSASVSDNGRGIPTDLHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGL
CCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGISVVNALSEWVEVTVRRQQQVHFQRFERGASIGSLKSENLSKSDKNLTGTTVCFKP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECC
DDQIFTDGISFEYGILSSRLRELAYLNGGVRIVFRDERKQSNDSSQTPHEEVYFYEGGIK
CCCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCHH
EYVEYMTKAKDSLHPEIIYVNSEKDGVQVEAAMQWCVDAYSDSILGFANNIRTIDGGTHI
HHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCH
EGLKTVLTRTLNSFAKKRGKRKDGDSNLAGENIREGLTAVLSVKVPDPEFEGQTKTKLGN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCEEEEEEECCCCCCCCCCHHCCCC
TEVRGIVDSLVGTSLSQYLEFNPNVIDLILEKAIQAFNAAEAARRARELVRRKSVLESST
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPGKLADCSSRDPSESEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDAKMY
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCHHH
KNTEIQSLITALGLGIKGEDFEVKNLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQKDLV
CCCHHHHHHHHHCCCCCCCCCEECCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
EGGYIYIACPPLYKVTRGKNHKYCYNESDMQKTLASFGEKANYTIQRFKGLGEMMPKQLW
CCCEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
DTTMDPTTRMMKRVEIEDALEADRIFTILMGDKVAPRREFIETHSVDLDLATLDI
CCCCCHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]