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The map label for this gene is thiC

Identifier: 124026768

GI number: 124026768

Start: 1708157

End: 1709557

Strand: Reverse

Name: thiC

Synonym: NATL1_20631

Alternate gene names: 124026768

Gene position: 1709557-1708157 (Counterclockwise)

Preceding gene: 124026769

Following gene: 124026764

Centisome position: 91.68

GC content: 39.47

Gene sequence:

>1401_bases
ATGCGGAATTCATGGGTGGCTTCGAGAAAAGGTAAAACCAATGTTTCTCAGATGCATTTTGCTCGCAAAGGCGAAATTAC
TGAAGAAATGAGGTATGTGGCAAAGCGTGAGAATCTTCCTGAGTCTCTGGTTATGGAAGAAGTCGCGCGCGGTCGAATGA
TTATTCCTGCAAATATTAACCATATAAACTTAGAGCCGATGGCAATAGGTATTGCCTCAACATGTAAAGTCAATGCAAAT
ATTGGTGCTTCACCAAATGCAAGCGATATCAGTGAAGAATTAAAGAAGCTTGATCTAGCAGTAAAATATGGGGCTGATAC
TCTTATGGATCTTTCTACTGGAGGGGTTAATTTAGATGAGGTGCGAACTGAAATTATCAATGCCTCCCCTATCCCGATAG
GGACAGTTCCTGTTTATCAAGCTTTAGAGAGTGTTCACGGTTCTATTTCAAGGTTAAATGAGGATGATTTTTTACACATT
ATAGAAAAGCATTGTCAGCAGGGAGTTGATTATCAAACCATTCATGCAGGCTTATTGATTGAACATTTACCCAAGGTTAA
AGGTCGTATTACTGGAATAGTTAGTCGTGGCGGAGGAATTCTTGCCCAATGGATGCTTTATCACTACAAACAAAATCCTT
TGTTTACTCGTTTTGATGATATTTGTGAAATTTTTAAACGCTATGACTGCACCTTTTCTTTAGGTGACTCTCTAAGGCCT
GGATGTCTGCATGATGCATCAGATGAAGCTCAACTCGCTGAGTTGAAAACTCTAGGTGAATTGACTAGACGTGCTTGGAA
GCATGATGTTCAAGTCATGGTTGAAGGGCCTGGTCATGTACCTATGGATCAAATCGAATTCAATGTTAGGAAGCAAATGG
AGGAGTGTTCAGAAGCTCCCTTTTATGTTCTAGGTCCATTGGTAACAGATATTTCTCCTGGTTATGATCACATTTCAAGT
GCTATTGGTGCAGCCATGGCAGGTTGGTACGGGACTGCGATGCTTTGTTATGTAACACCTAAGGAACATCTTGGGTTGCC
TAATCCTGAGGATGTTAGAGAAGGTTTAATTGCTTATAAAATTGCTGCTCATGCCGCAGATGTCGCAAGACATAGATCAG
GAGCACGTGATCGTGATGATGAATTAAGTAAGGCTCGTAAAGAATTTGACTGGAACAAACAGTTTGAATTGTCCTTAGAT
CCAGAAAAAGCTAAGCAATATCATGACGAAACTTTACCTGAAGAAATTTTCAAGAAAGCAGAGTTTTGTTCAATGTGTGG
TCCTAATCATTGTCCAATGAATACAAAAATCACAGATGAAGATCTTGATCAATTAAACGATCAAATACAGTCAAAAGGTG
CAGCTGAATTAACTCCAGTAAAGTTAAACAAAGAAAACTAG

Upstream 100 bases:

>100_bases
CACCCTCTGAACTTGATCTGGTTTAAACCAGTGTAAGGAAAGTGAAATCTTGTGATCTTGATGCGTATTGCTCCTTTTTT
ATTCAATAGCTTTTTAGTTC

Downstream 100 bases:

>100_bases
TTCTTTGTTTAACTTTTTCTGTAATAACTATTTGTAGTTAAATAATTAATTTTATTTGTTGAGCAGACTTTTAGATTTTT
CTAGTACGTTTTCAACTGTA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MRNSWVASRKGKTNVSQMHFARKGEITEEMRYVAKRENLPESLVMEEVARGRMIIPANINHINLEPMAIGIASTCKVNAN
IGASPNASDISEELKKLDLAVKYGADTLMDLSTGGVNLDEVRTEIINASPIPIGTVPVYQALESVHGSISRLNEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPKVKGRITGIVSRGGGILAQWMLYHYKQNPLFTRFDDICEIFKRYDCTFSLGDSLRP
GCLHDASDEAQLAELKTLGELTRRAWKHDVQVMVEGPGHVPMDQIEFNVRKQMEECSEAPFYVLGPLVTDISPGYDHISS
AIGAAMAGWYGTAMLCYVTPKEHLGLPNPEDVREGLIAYKIAAHAADVARHRSGARDRDDELSKARKEFDWNKQFELSLD
PEKAKQYHDETLPEEIFKKAEFCSMCGPNHCPMNTKITDEDLDQLNDQIQSKGAAELTPVKLNKEN

Sequences:

>Translated_466_residues
MRNSWVASRKGKTNVSQMHFARKGEITEEMRYVAKRENLPESLVMEEVARGRMIIPANINHINLEPMAIGIASTCKVNAN
IGASPNASDISEELKKLDLAVKYGADTLMDLSTGGVNLDEVRTEIINASPIPIGTVPVYQALESVHGSISRLNEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPKVKGRITGIVSRGGGILAQWMLYHYKQNPLFTRFDDICEIFKRYDCTFSLGDSLRP
GCLHDASDEAQLAELKTLGELTRRAWKHDVQVMVEGPGHVPMDQIEFNVRKQMEECSEAPFYVLGPLVTDISPGYDHISS
AIGAAMAGWYGTAMLCYVTPKEHLGLPNPEDVREGLIAYKIAAHAADVARHRSGARDRDDELSKARKEFDWNKQFELSLD
PEKAKQYHDETLPEEIFKKAEFCSMCGPNHCPMNTKITDEDLDQLNDQIQSKGAAELTPVKLNKEN
>Mature_466_residues
MRNSWVASRKGKTNVSQMHFARKGEITEEMRYVAKRENLPESLVMEEVARGRMIIPANINHINLEPMAIGIASTCKVNAN
IGASPNASDISEELKKLDLAVKYGADTLMDLSTGGVNLDEVRTEIINASPIPIGTVPVYQALESVHGSISRLNEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPKVKGRITGIVSRGGGILAQWMLYHYKQNPLFTRFDDICEIFKRYDCTFSLGDSLRP
GCLHDASDEAQLAELKTLGELTRRAWKHDVQVMVEGPGHVPMDQIEFNVRKQMEECSEAPFYVLGPLVTDISPGYDHISS
AIGAAMAGWYGTAMLCYVTPKEHLGLPNPEDVREGLIAYKIAAHAADVARHRSGARDRDDELSKARKEFDWNKQFELSLD
PEKAKQYHDETLPEEIFKKAEFCSMCGPNHCPMNTKITDEDLDQLNDQIQSKGAAELTPVKLNKEN

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=476, Percent_Identity=54.6218487394958, Blast_Score=514, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_PROM1 (A2C559)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015883.1
- STRING:   A2C559
- GeneID:   4780043
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_20631
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   EPMAIGI
- ProtClustDB:   PRK13352
- BioCyc:   PMAR167555:NATL1_20631-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 52070; Mature: 52070

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: BINDING 80-80 BINDING 109-109 BINDING 139-139 BINDING 175-175 BINDING 275-275 BINDING 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNSWVASRKGKTNVSQMHFARKGEITEEMRYVAKRENLPESLVMEEVARGRMIIPANIN
CCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HINLEPMAIGIASTCKVNANIGASPNASDISEELKKLDLAVKYGADTLMDLSTGGVNLDE
EEECCHHEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHH
VRTEIINASPIPIGTVPVYQALESVHGSISRLNEDDFLHIIEKHCQQGVDYQTIHAGLLI
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
EHLPKVKGRITGIVSRGGGILAQWMLYHYKQNPLFTRFDDICEIFKRYDCTFSLGDSLRP
HHHHHHHHHHEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECCCCCCC
GCLHDASDEAQLAELKTLGELTRRAWKHDVQVMVEGPGHVPMDQIEFNVRKQMEECSEAP
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDISPGYDHISSAIGAAMAGWYGTAMLCYVTPKEHLGLPNPEDVREGLIAYK
EEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHHHHHH
IAAHAADVARHRSGARDRDDELSKARKEFDWNKQFELSLDPEKAKQYHDETLPEEIFKKA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHCCCHHHHHHHH
EFCSMCGPNHCPMNTKITDEDLDQLNDQIQSKGAAELTPVKLNKEN
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCC
>Mature Secondary Structure
MRNSWVASRKGKTNVSQMHFARKGEITEEMRYVAKRENLPESLVMEEVARGRMIIPANIN
CCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HINLEPMAIGIASTCKVNANIGASPNASDISEELKKLDLAVKYGADTLMDLSTGGVNLDE
EEECCHHEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHH
VRTEIINASPIPIGTVPVYQALESVHGSISRLNEDDFLHIIEKHCQQGVDYQTIHAGLLI
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
EHLPKVKGRITGIVSRGGGILAQWMLYHYKQNPLFTRFDDICEIFKRYDCTFSLGDSLRP
HHHHHHHHHHEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECCCCCCC
GCLHDASDEAQLAELKTLGELTRRAWKHDVQVMVEGPGHVPMDQIEFNVRKQMEECSEAP
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDISPGYDHISSAIGAAMAGWYGTAMLCYVTPKEHLGLPNPEDVREGLIAYK
EEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHHHHHH
IAAHAADVARHRSGARDRDDELSKARKEFDWNKQFELSLDPEKAKQYHDETLPEEIFKKA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHCCCHHHHHHHH
EFCSMCGPNHCPMNTKITDEDLDQLNDQIQSKGAAELTPVKLNKEN
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA