Click here to switch to the map view.

The map label for this gene is prfA

Identifier: 124026680

GI number: 124026680

Start: 1631087

End: 1632184

Strand: Reverse

Name: prfA

Synonym: NATL1_19751

Alternate gene names: 124026680

Gene position: 1632184-1631087 (Counterclockwise)

Preceding gene: 124026681

Following gene: 161407967

Centisome position: 87.53

GC content: 36.52

Gene sequence:

>1098_bases
ATGGATTCTTCAACCCTAATTAAAAGATTAGAAACAGCTAAAAGTAGTTTTCAAAACTTGGAGTTGCAATTAGCTGATCC
AGATGTTGCATCAGATCCAAAACAATTAGAGACAATTGCTAGAGAAAGATCCCGTTTAGAGCCTTTGGTTAATGATTATT
TAAAGCTCCAGATAATAGAAAAAGAGTGTCTAGAAGCTAAAGGATTAGTAAAAGAAAGCAGAGATGATAAAGAGATGGAA
CTTTTAGCTAGAGAAGAACTTCAAAATCTCGAACTCTCAAAAAATGAATTAATTCAAAAGTTGACTTTAGCTCTTTTACC
CAAAGATCCAAGAGATGAAAGAAGTGTGATGCTTGAAATCAGAGCTGGAGCAGGAGGAAATGAAGCTTGTTTATGGGCTG
GTGATTTAGCAAGAATGTATGAAAGATATGGTCAAAAACTTGGATGGAATGTAAAACCTATAAGTTCAACAGAAGCTGAT
ATGGGCGGTTTTCGTGAAATGATTATCTCTGTCAAAGGAGAGTCTGTTTACAGCCAATTAAAATTTGAAGCAGGTGTTCA
TAGAGTTCAAAGAGTCCCTTCAACTGAATCTCAAGGACGAGTTCATACCTCTACTGCAACTGTTGCTGTAATGCCAGAAG
CTGATCCAGTAGAGGTGAAAATAGAATCTACTGATCTAGAAATTAGTACGGCAAGATCAGGGGGTGCTGGCGGGCAAAAT
GTAAATAAAGTAGAAACAGCTATTGATTTATTTCACAAGCCCTCAGGTATAAGAGTTTTTTGTACGCAAGAACGTTCTCA
AATGCAAAATAGAGAAAGAGCAATGGAGATTTTAAGAGCTAAATTATTAGAACTAGAAATAGCTGAGGCTAATGCAAAGG
AAAGATCTGCTCGATTGTCTCAGGTTGGCACAGGTGATAGAAGTGAAAAGATTAGGACATATAACTACAAAGATAATCGA
ACAACTGATCATCGATTAGGAGTTAATTTCCCCCTCGAACAAGTTCTTTCAGGTCAATTGGAAGATGTCATAGGTGCATG
TATTGCAGAAGAACAAAAAAGGCAAATGGAAGAGCTTAGTAATCAATCTGAAGATTAA

Upstream 100 bases:

>100_bases
GTATGGCCTCATCTGACTCAAGTGAACAAAAAGATAAATCTTCAGAAGAAAAAAAAGAAAGTTGACAGCTGTTGATCTAA
AGATTTAGGGAATAAAAAGA

Downstream 100 bases:

>100_bases
AAGCTAAGCTACCCATCCAATTACGTATTTACTCCATTCTTTATGACGACCAGAATGAATTTGTTTTGTTGTTTCAAAAC
TCATGTTATTTAAAGGTTTA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MDSSTLIKRLETAKSSFQNLELQLADPDVASDPKQLETIARERSRLEPLVNDYLKLQIIEKECLEAKGLVKESRDDKEME
LLAREELQNLELSKNELIQKLTLALLPKDPRDERSVMLEIRAGAGGNEACLWAGDLARMYERYGQKLGWNVKPISSTEAD
MGGFREMIISVKGESVYSQLKFEAGVHRVQRVPSTESQGRVHTSTATVAVMPEADPVEVKIESTDLEISTARSGGAGGQN
VNKVETAIDLFHKPSGIRVFCTQERSQMQNRERAMEILRAKLLELEIAEANAKERSARLSQVGTGDRSEKIRTYNYKDNR
TTDHRLGVNFPLEQVLSGQLEDVIGACIAEEQKRQMEELSNQSED

Sequences:

>Translated_365_residues
MDSSTLIKRLETAKSSFQNLELQLADPDVASDPKQLETIARERSRLEPLVNDYLKLQIIEKECLEAKGLVKESRDDKEME
LLAREELQNLELSKNELIQKLTLALLPKDPRDERSVMLEIRAGAGGNEACLWAGDLARMYERYGQKLGWNVKPISSTEAD
MGGFREMIISVKGESVYSQLKFEAGVHRVQRVPSTESQGRVHTSTATVAVMPEADPVEVKIESTDLEISTARSGGAGGQN
VNKVETAIDLFHKPSGIRVFCTQERSQMQNRERAMEILRAKLLELEIAEANAKERSARLSQVGTGDRSEKIRTYNYKDNR
TTDHRLGVNFPLEQVLSGQLEDVIGACIAEEQKRQMEELSNQSED
>Mature_365_residues
MDSSTLIKRLETAKSSFQNLELQLADPDVASDPKQLETIARERSRLEPLVNDYLKLQIIEKECLEAKGLVKESRDDKEME
LLAREELQNLELSKNELIQKLTLALLPKDPRDERSVMLEIRAGAGGNEACLWAGDLARMYERYGQKLGWNVKPISSTEAD
MGGFREMIISVKGESVYSQLKFEAGVHRVQRVPSTESQGRVHTSTATVAVMPEADPVEVKIESTDLEISTARSGGAGGQN
VNKVETAIDLFHKPSGIRVFCTQERSQMQNRERAMEILRAKLLELEIAEANAKERSARLSQVGTGDRSEKIRTYNYKDNR
TTDHRLGVNFPLEQVLSGQLEDVIGACIAEEQKRQMEELSNQSED

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=267, Percent_Identity=41.9475655430712, Blast_Score=224, Evalue=7e-59,
Organism=Homo sapiens, GI34577120, Length=329, Percent_Identity=37.0820668693009, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI166795305, Length=196, Percent_Identity=38.265306122449, Blast_Score=151, Evalue=8e-37,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=41.3888888888889, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI2367172, Length=341, Percent_Identity=34.3108504398827, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17542784, Length=304, Percent_Identity=36.5131578947368, Blast_Score=188, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=329, Percent_Identity=39.5136778115502, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI19921226, Length=329, Percent_Identity=37.3860182370821, Blast_Score=211, Evalue=4e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_PROM1 (A2C4X1)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015795.1
- ProteinModelPortal:   A2C4X1
- SMR:   A2C4X1
- STRING:   A2C4X1
- GeneID:   4780558
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_19751
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   PMAR167555:NATL1_19751-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40977; Mature: 40977

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSSTLIKRLETAKSSFQNLELQLADPDVASDPKQLETIARERSRLEPLVNDYLKLQIIE
CCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHEEHHH
KECLEAKGLVKESRDDKEMELLAREELQNLELSKNELIQKLTLALLPKDPRDERSVMLEI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEE
RAGAGGNEACLWAGDLARMYERYGQKLGWNVKPISSTEADMGGFREMIISVKGESVYSQL
ECCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
KFEAGVHRVQRVPSTESQGRVHTSTATVAVMPEADPVEVKIESTDLEISTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCEEEEEECCCEEEEECCCCCCCCCC
VNKVETAIDLFHKPSGIRVFCTQERSQMQNRERAMEILRAKLLELEIAEANAKERSARLS
HHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QVGTGDRSEKIRTYNYKDNRTTDHRLGVNFPLEQVLSGQLEDVIGACIAEEQKRQMEELS
HCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
NQSED
CCCCC
>Mature Secondary Structure
MDSSTLIKRLETAKSSFQNLELQLADPDVASDPKQLETIARERSRLEPLVNDYLKLQIIE
CCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHEEHHH
KECLEAKGLVKESRDDKEMELLAREELQNLELSKNELIQKLTLALLPKDPRDERSVMLEI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEE
RAGAGGNEACLWAGDLARMYERYGQKLGWNVKPISSTEADMGGFREMIISVKGESVYSQL
ECCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
KFEAGVHRVQRVPSTESQGRVHTSTATVAVMPEADPVEVKIESTDLEISTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCEEEEEECCCEEEEECCCCCCCCCC
VNKVETAIDLFHKPSGIRVFCTQERSQMQNRERAMEILRAKLLELEIAEANAKERSARLS
HHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QVGTGDRSEKIRTYNYKDNRTTDHRLGVNFPLEQVLSGQLEDVIGACIAEEQKRQMEELS
HCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
NQSED
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA