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The map label for this gene is pheA [H]

Identifier: 124026650

GI number: 124026650

Start: 1597927

End: 1598757

Strand: Direct

Name: pheA [H]

Synonym: NATL1_19451

Alternate gene names: 124026650

Gene position: 1597927-1598757 (Clockwise)

Preceding gene: 124026648

Following gene: 124026658

Centisome position: 85.69

GC content: 37.91

Gene sequence:

>831_bases
ATGTCAACTCGAGTGGCCTATCTAGGGCCTAAAGGAACATACGCTGAAAAAGCCGCGAAAGCGCTAGCAGAGCTTGAGAA
GCTTGAATCTCCAGTATTTTCCCCATGCAAAGGGTTAAGGTCTGTGGTAGATAATCTTGCAAACAATCTTTGTGAAGCAG
CCGTAGTACCAATTGAAAATTCAGTAGAAGGTGGGGTCACCACAACATTGGATTCTCTATGGAGACATGAAAACCTATTT
ATACATAGAGCCCTAGTACTCCCTATTAAACACTCATTAATGAGTAGCGGATCAATTTCAAAGATTTCAGAAGTCCTTTC
CCATCCTCAGGCAATAGCACAATGCAGCCAGTGGTTAAATGACAATATTCCAAATGCAGTTCATCTACCAACTAATTCAA
CGGCAGAAGCGGTAAGAATGGTAAAAGGCAGCTTATTTAGAGCAGCGATAGGATCCAAAGATGCAAGTGAGGATCTGAAT
ATTCTTGCTTATCCTATTAATGATATTGAAGGGAATTGCACTAGATTCGTTCTTCTCAGTAAGAACAACATCAAAGTGAA
TGGGAATAGGGCAAGTATGGCGTTTTCTCTTAAGTCGAATAGCCCGGGTGCATTACTAAAAGCATTGAATTGCATCGCAA
ATCTTGGCTTGAATATGAGTAGAATTGAATCACGTCCTTCTAAAAGAGAACTTGGTGAATATGTCTTTTTCATAGATCTT
GATTTAAACAATAATAATAAAAAAGACTATAAAAACATTACTAAAGAATTATCTCCTCTTTGCAATCAAATAATTAATTT
TGGATGCTATTTCGGATCAGAAGTTGAGTAA

Upstream 100 bases:

>100_bases
AACAACTAGATCAATTTTTTGTCGTGCTGTAAAGGCAAGAGCCATTATCAAAAACTTGCTCAGTTTGATCTTATCGGTTT
TTACTCCTTTTTTTTTTCTA

Downstream 100 bases:

>100_bases
GTTATCCTCTTGATTTGAATACTCCAAATTTCATCAAACCTCTTGAAAAAGACCATCTCATCATTAGTATTGTTGGGATT
TCTTTAAATGCCTTTAGTAA

Product: chorismate mutase-prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSTRVAYLGPKGTYAEKAAKALAELEKLESPVFSPCKGLRSVVDNLANNLCEAAVVPIENSVEGGVTTTLDSLWRHENLF
IHRALVLPIKHSLMSSGSISKISEVLSHPQAIAQCSQWLNDNIPNAVHLPTNSTAEAVRMVKGSLFRAAIGSKDASEDLN
ILAYPINDIEGNCTRFVLLSKNNIKVNGNRASMAFSLKSNSPGALLKALNCIANLGLNMSRIESRPSKRELGEYVFFIDL
DLNNNNKKDYKNITKELSPLCNQIINFGCYFGSEVE

Sequences:

>Translated_276_residues
MSTRVAYLGPKGTYAEKAAKALAELEKLESPVFSPCKGLRSVVDNLANNLCEAAVVPIENSVEGGVTTTLDSLWRHENLF
IHRALVLPIKHSLMSSGSISKISEVLSHPQAIAQCSQWLNDNIPNAVHLPTNSTAEAVRMVKGSLFRAAIGSKDASEDLN
ILAYPINDIEGNCTRFVLLSKNNIKVNGNRASMAFSLKSNSPGALLKALNCIANLGLNMSRIESRPSKRELGEYVFFIDL
DLNNNNKKDYKNITKELSPLCNQIINFGCYFGSEVE
>Mature_275_residues
STRVAYLGPKGTYAEKAAKALAELEKLESPVFSPCKGLRSVVDNLANNLCEAAVVPIENSVEGGVTTTLDSLWRHENLFI
HRALVLPIKHSLMSSGSISKISEVLSHPQAIAQCSQWLNDNIPNAVHLPTNSTAEAVRMVKGSLFRAAIGSKDASEDLNI
LAYPINDIEGNCTRFVLLSKNNIKVNGNRASMAFSLKSNSPGALLKALNCIANLGLNMSRIESRPSKRELGEYVFFIDLD
LNNNNKKDYKNITKELSPLCNQIINFGCYFGSEVE

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=277, Percent_Identity=33.2129963898917, Blast_Score=119, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324013, Length=324, Percent_Identity=27.1604938271605, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010957
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01842 ACT; PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 30155; Mature: 30024

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRVAYLGPKGTYAEKAAKALAELEKLESPVFSPCKGLRSVVDNLANNLCEAAVVPIEN
CCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCC
SVEGGVTTTLDSLWRHENLFIHRALVLPIKHSLMSSGSISKISEVLSHPQAIAQCSQWLN
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHH
DNIPNAVHLPTNSTAEAVRMVKGSLFRAAIGSKDASEDLNILAYPINDIEGNCTRFVLLS
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEE
KNNIKVNGNRASMAFSLKSNSPGALLKALNCIANLGLNMSRIESRPSKRELGEYVFFIDL
CCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCEEEEEEE
DLNNNNKKDYKNITKELSPLCNQIINFGCYFGSEVE
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STRVAYLGPKGTYAEKAAKALAELEKLESPVFSPCKGLRSVVDNLANNLCEAAVVPIEN
CCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCC
SVEGGVTTTLDSLWRHENLFIHRALVLPIKHSLMSSGSISKISEVLSHPQAIAQCSQWLN
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHH
DNIPNAVHLPTNSTAEAVRMVKGSLFRAAIGSKDASEDLNILAYPINDIEGNCTRFVLLS
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEE
KNNIKVNGNRASMAFSLKSNSPGALLKALNCIANLGLNMSRIESRPSKRELGEYVFFIDL
CCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCEEEEEEE
DLNNNNKKDYKNITKELSPLCNQIINFGCYFGSEVE
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]