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The map label for this gene is nusA [H]

Identifier: 124026598

GI number: 124026598

Start: 1555126

End: 1556619

Strand: Direct

Name: nusA [H]

Synonym: NATL1_18931

Alternate gene names: 124026598

Gene position: 1555126-1556619 (Clockwise)

Preceding gene: 124026597

Following gene: 124026599

Centisome position: 83.4

GC content: 39.89

Gene sequence:

>1494_bases
ATGGCTCTTGTTCTACTCCCAGGCTTAAATAACTTAATTGAAGACATTAGTGAGGAAAAAAAGCTTCCATCACAAGTCGT
CGAAGCTGCTTTGAGAGAAGCACTTCTAAAAGGTTATGAACGATATCGAAGAACTCTTTATTTAGGTATCAGCGAAGATC
CTTTTGAAGAAGATTATTTCAGCAACTTTGATGTTGGTTTAGATCTAGAAGAGGAGGGGTATAGAGTTTTAGCCAGTAAA
ATAATAGTTGAGGAGGTAGAGAGTGATGATCATCAAATAGCAATAGCAGAGGTTATGCAAGTTGCTGATGATGCTCAAGT
TGGCGATACCGTTGTACTAGATGTGACTCCAGAGAAAGAAGATTTTGGAAGAATGGCTGCGGCTACAACCAAGCAGGTTT
TAGCCCAAAAATTGAGAGATCAGCAAAGAAGAATGATTCAAGAGGAATTTGCTGACCTAGAAGATCCAGTGCTAACTGCC
AGGGTCATTAGATTTGAAAGGCAGTCAGTAATTATGGCAGTAAGCTCTGGTTTAGGTAGACCAGAAGTAGAAGCAGAGCT
TCCAAGACGAGATCAATTGCCAAATGATAATTACCGAGCAAACGCGACATTTAAAGTATTTCTTAAAGAAGTTAGTGAAA
CGCCTAGGAGAGGTCCACAACTTTTTGTTAGCAGGTCAAATGCTGGATTGGTTGTATATCTGTTTGAGAATGAAGTTCCT
GAAATTCAAGAAGGTTCAGTACGTATAGTTGCTGTCGCTCGTGAAGCAAATCCACCTTCAAGAGCAGTAGGTCCCAGAAC
AAAAGTAGCTGTTGACAGTATTGAAAGAGAGGTTGACCCCGTTGGAGCATGTATTGGTGCTAGAGGTGCTCGAATTCAAC
AAGTTGTAAATGAACTTCGTGGAGAAAAAATTGATGTAATTCGTTGGTCTTCAGACCCTGTTCAATACATCTGTAATTCA
TTAAGTCCTGCAAGAGTTGAAGTAGTTAGACTTGTGGATCCAGAAGGTCAGCATGCTCATGTCTTAGTTCCTCCAGATCA
ACTTAGCCTCGCAATTGGAAGAGAAGGGCAAAATGTAAGACTTGCTGCAAGGCTTACAGGATGGAAAATAGATATCAAAA
ATTCACAAGAATATGATCAAGCAACTGAAGACTCGGAGGTTGCGGAATTAATTTCTCAAAGAGAAGAAGAAGAATCTTTA
CAGAGAGAAGCTGAGAAGAGATTAGAGGCAGAACAGACAGCTAGAGCTGAGGAAGATGCACGCCTAAGAGAGCTTTACCC
CCTCCCAGAGGATGAAGAAGACTTTCAAAATGAGGAATTGTCAAACATCGATAGCAATGAAACAGAATCTGAATCTGATT
TTGAATCTTCTGAAATTGATTCAACTATCGATACTGATGACACTGAAACTGAAGAAAGTGATATTGATGAAACAATCGAA
GACACAGTTACTGCTAAAGATAAAATAATTACAGAAGAGGAAGGAACCCGGTGA

Upstream 100 bases:

>100_bases
GTCGAAGACGTTATTCAAGTCCGACTCACAACACCCGCTGGTTAAAATCTAGATAGTAATTATCTAACCCAATCATTTTC
ATCCCTATATCCCATCATCG

Downstream 100 bases:

>100_bases
GTCAACCCCCCATTCTGCGTAGGTGTGTAGCTTGCAAAAAAGTTCTTGATCGCAAGTATTTTTTAAAAGTTACTAGAGAT
TTTCAGAATGGAGTAGTTTT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MALVLLPGLNNLIEDISEEKKLPSQVVEAALREALLKGYERYRRTLYLGISEDPFEEDYFSNFDVGLDLEEEGYRVLASK
IIVEEVESDDHQIAIAEVMQVADDAQVGDTVVLDVTPEKEDFGRMAAATTKQVLAQKLRDQQRRMIQEEFADLEDPVLTA
RVIRFERQSVIMAVSSGLGRPEVEAELPRRDQLPNDNYRANATFKVFLKEVSETPRRGPQLFVSRSNAGLVVYLFENEVP
EIQEGSVRIVAVAREANPPSRAVGPRTKVAVDSIEREVDPVGACIGARGARIQQVVNELRGEKIDVIRWSSDPVQYICNS
LSPARVEVVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDIKNSQEYDQATEDSEVAELISQREEEESL
QREAEKRLEAEQTARAEEDARLRELYPLPEDEEDFQNEELSNIDSNETESESDFESSEIDSTIDTDDTETEESDIDETIE
DTVTAKDKIITEEEGTR

Sequences:

>Translated_497_residues
MALVLLPGLNNLIEDISEEKKLPSQVVEAALREALLKGYERYRRTLYLGISEDPFEEDYFSNFDVGLDLEEEGYRVLASK
IIVEEVESDDHQIAIAEVMQVADDAQVGDTVVLDVTPEKEDFGRMAAATTKQVLAQKLRDQQRRMIQEEFADLEDPVLTA
RVIRFERQSVIMAVSSGLGRPEVEAELPRRDQLPNDNYRANATFKVFLKEVSETPRRGPQLFVSRSNAGLVVYLFENEVP
EIQEGSVRIVAVAREANPPSRAVGPRTKVAVDSIEREVDPVGACIGARGARIQQVVNELRGEKIDVIRWSSDPVQYICNS
LSPARVEVVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDIKNSQEYDQATEDSEVAELISQREEEESL
QREAEKRLEAEQTARAEEDARLRELYPLPEDEEDFQNEELSNIDSNETESESDFESSEIDSTIDTDDTETEESDIDETIE
DTVTAKDKIITEEEGTR
>Mature_496_residues
ALVLLPGLNNLIEDISEEKKLPSQVVEAALREALLKGYERYRRTLYLGISEDPFEEDYFSNFDVGLDLEEEGYRVLASKI
IVEEVESDDHQIAIAEVMQVADDAQVGDTVVLDVTPEKEDFGRMAAATTKQVLAQKLRDQQRRMIQEEFADLEDPVLTAR
VIRFERQSVIMAVSSGLGRPEVEAELPRRDQLPNDNYRANATFKVFLKEVSETPRRGPQLFVSRSNAGLVVYLFENEVPE
IQEGSVRIVAVAREANPPSRAVGPRTKVAVDSIEREVDPVGACIGARGARIQQVVNELRGEKIDVIRWSSDPVQYICNSL
SPARVEVVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDIKNSQEYDQATEDSEVAELISQREEEESLQ
REAEKRLEAEQTARAEEDARLRELYPLPEDEEDFQNEELSNIDSNETESESDFESSEIDSTIDTDDTETEESDIDETIED
TVTAKDKIITEEEGTR

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=361, Percent_Identity=33.5180055401662, Blast_Score=181, Evalue=7e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55973; Mature: 55841

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVLLPGLNNLIEDISEEKKLPSQVVEAALREALLKGYERYRRTLYLGISEDPFEEDYF
CEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHH
SNFDVGLDLEEEGYRVLASKIIVEEVESDDHQIAIAEVMQVADDAQVGDTVVLDVTPEKE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCEEEEEECCCHH
DFGRMAAATTKQVLAQKLRDQQRRMIQEEFADLEDPVLTARVIRFERQSVIMAVSSGLGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PEVEAELPRRDQLPNDNYRANATFKVFLKEVSETPRRGPQLFVSRSNAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCC
EIQEGSVRIVAVAREANPPSRAVGPRTKVAVDSIEREVDPVGACIGARGARIQQVVNELR
CCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GEKIDVIRWSSDPVQYICNSLSPARVEVVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCHHEEEEECCCCCCEE
LAARLTGWKIDIKNSQEYDQATEDSEVAELISQREEEESLQREAEKRLEAEQTARAEEDA
EEEEEECEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RLRELYPLPEDEEDFQNEELSNIDSNETESESDFESSEIDSTIDTDDTETEESDIDETIE
HHHHHCCCCCCHHHHCHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHH
DTVTAKDKIITEEEGTR
HHHHHHHHEECCCCCCC
>Mature Secondary Structure 
ALVLLPGLNNLIEDISEEKKLPSQVVEAALREALLKGYERYRRTLYLGISEDPFEEDYF
EEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHH
SNFDVGLDLEEEGYRVLASKIIVEEVESDDHQIAIAEVMQVADDAQVGDTVVLDVTPEKE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCEEEEEECCCHH
DFGRMAAATTKQVLAQKLRDQQRRMIQEEFADLEDPVLTARVIRFERQSVIMAVSSGLGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PEVEAELPRRDQLPNDNYRANATFKVFLKEVSETPRRGPQLFVSRSNAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCC
EIQEGSVRIVAVAREANPPSRAVGPRTKVAVDSIEREVDPVGACIGARGARIQQVVNELR
CCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GEKIDVIRWSSDPVQYICNSLSPARVEVVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCHHEEEEECCCCCCEE
LAARLTGWKIDIKNSQEYDQATEDSEVAELISQREEEESLQREAEKRLEAEQTARAEEDA
EEEEEECEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RLRELYPLPEDEEDFQNEELSNIDSNETESESDFESSEIDSTIDTDDTETEESDIDETIE
HHHHHCCCCCCHHHHCHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHH
DTVTAKDKIITEEEGTR
HHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]