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The map label for this gene is rlmN

Identifier: 124026587

GI number: 124026587

Start: 1539004

End: 1540083

Strand: Reverse

Name: rlmN

Synonym: NATL1_18821

Alternate gene names: 124026587

Gene position: 1540083-1539004 (Counterclockwise)

Preceding gene: 124026588

Following gene: 124026586

Centisome position: 82.59

GC content: 38.15

Gene sequence:

>1080_bases
GTGACAAAACTTCCTAAACTATCAAGTAATTCATCCTTGCTTGGCTTAAGTTCGGAAGATCTTGAAGAATTTGCTCGTCA
GGAAGGTGAAAAGTCTTTTCGTGGTAGGCAAATTCATGAATGGATTTATCAAAGAGGGGCAAAAAGCTTAGATTCAATAA
GTGTTCTTCCAAAAAAATGGCGAGATTCATTAGTACGTAAGGGAATTCAGATTGGAAGGCTGGACGAAATTAATAGAGTT
GTAGCCGAAGATGAGACATTGAAATTATTAATGGGGACTTTTGATGGTGAGATTGTAGAAACAGTAGGAATACCAACAGA
TAAAAGACTTACTGTTTGTGTCTCAAGTCAAATTGGCTGCCCAATGGGTTGCAAATTTTGTGCGACTGGGAAGGGGGGGC
TTAATAGATCTCTTGATGTGAATGAGATAGTTGATCAAGTTATTAGTGTTAGAGAAACAATGAATAGGAGGCCTACTCAT
GTCGTGTTTATGGGTATGGGCGAGCCACTCCTAAATATTCAGAATGTTCTTGACTCTATAGAATGTCTCACAAGTGATAT
TGGTATTGGTCAAAGGAAGATAACGGTTAGTACTGTTGGGATACCGAATACTCTTTCAGATTTAGCAAAATTAGCTCAAG
ACCGTTTAGGAAGAGTTCAATTCACACTTGCAGTCAGTCTTCATGCACCTAATCAGACGTTGCGTGAATTGATAATCCCC
TCGGCGAGTTCATATCCAATTAATTCATTACTGAAAGACTGTAAAAAATATATAGATCTCACTGGTAGACGAGTAAGCTT
TGAGTATATACTTCTTGGCGGTTTGAATGACAAAGATATTCATGCAGAGCAGTTAGCTAATCTGATGAGAGGCTTTCAGA
GCCATGTTAATTTGATAGCTTATAATCCAATCGCTGAAGAGAACTTTAAGCGACCAAGCCAATCTAGAGTTAATGCCTTT
AGAGAGCTATTAGAAAATAGGGGAGTTGCTGTAAGTGTTCGTGCAAGTAGAGGTAGAGATAAAGATGCGGCATGTGGACA
ATTAAGAAGGCAAACAATCGATAAAATAAAAATCAACTAA

Upstream 100 bases:

>100_bases
TTCATACACATACTGAAAGAGTTAATGGCAGATGGGCAATGATTGGTTTTATTGCATTAGTTCTTTTGGAATTTAAATTG
GGACATGGCCTAATGAATTG

Downstream 100 bases:

>100_bases
GCAGGAAACATATATGTCTTGTGGACTCTTATGACATTAATTGATTGGTTTATTTTATTTGTTTATTTGATCTTTTCCTT
GGTATTGGGAATTTATATAT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTKLPKLSSNSSLLGLSSEDLEEFARQEGEKSFRGRQIHEWIYQRGAKSLDSISVLPKKWRDSLVRKGIQIGRLDEINRV
VAEDETLKLLMGTFDGEIVETVGIPTDKRLTVCVSSQIGCPMGCKFCATGKGGLNRSLDVNEIVDQVISVRETMNRRPTH
VVFMGMGEPLLNIQNVLDSIECLTSDIGIGQRKITVSTVGIPNTLSDLAKLAQDRLGRVQFTLAVSLHAPNQTLRELIIP
SASSYPINSLLKDCKKYIDLTGRRVSFEYILLGGLNDKDIHAEQLANLMRGFQSHVNLIAYNPIAEENFKRPSQSRVNAF
RELLENRGVAVSVRASRGRDKDAACGQLRRQTIDKIKIN

Sequences:

>Translated_359_residues
MTKLPKLSSNSSLLGLSSEDLEEFARQEGEKSFRGRQIHEWIYQRGAKSLDSISVLPKKWRDSLVRKGIQIGRLDEINRV
VAEDETLKLLMGTFDGEIVETVGIPTDKRLTVCVSSQIGCPMGCKFCATGKGGLNRSLDVNEIVDQVISVRETMNRRPTH
VVFMGMGEPLLNIQNVLDSIECLTSDIGIGQRKITVSTVGIPNTLSDLAKLAQDRLGRVQFTLAVSLHAPNQTLRELIIP
SASSYPINSLLKDCKKYIDLTGRRVSFEYILLGGLNDKDIHAEQLANLMRGFQSHVNLIAYNPIAEENFKRPSQSRVNAF
RELLENRGVAVSVRASRGRDKDAACGQLRRQTIDKIKIN
>Mature_358_residues
TKLPKLSSNSSLLGLSSEDLEEFARQEGEKSFRGRQIHEWIYQRGAKSLDSISVLPKKWRDSLVRKGIQIGRLDEINRVV
AEDETLKLLMGTFDGEIVETVGIPTDKRLTVCVSSQIGCPMGCKFCATGKGGLNRSLDVNEIVDQVISVRETMNRRPTHV
VFMGMGEPLLNIQNVLDSIECLTSDIGIGQRKITVSTVGIPNTLSDLAKLAQDRLGRVQFTLAVSLHAPNQTLRELIIPS
ASSYPINSLLKDCKKYIDLTGRRVSFEYILLGGLNDKDIHAEQLANLMRGFQSHVNLIAYNPIAEENFKRPSQSRVNAFR
ELLENRGVAVSVRASRGRDKDAACGQLRRQTIDKIKIN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=358, Percent_Identity=37.4301675977654, Blast_Score=238, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_PROM1 (A2C4M8)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015702.1
- ProteinModelPortal:   A2C4M8
- STRING:   A2C4M8
- GeneID:   4780092
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_18821
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14457
- BioCyc:   PMAR167555:NATL1_18821-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39962; Mature: 39830

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLPKLSSNSSLLGLSSEDLEEFARQEGEKSFRGRQIHEWIYQRGAKSLDSISVLPKKW
CCCCCCCCCCCCEECCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCHHHHHHCHHHH
RDSLVRKGIQIGRLDEINRVVAEDETLKLLMGTFDGEIVETVGIPTDKRLTVCVSSQIGC
HHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCCCCCEEEEEECCCCCC
PMGCKFCATGKGGLNRSLDVNEIVDQVISVRETMNRRPTHVVFMGMGEPLLNIQNVLDSI
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
ECLTSDIGIGQRKITVSTVGIPNTLSDLAKLAQDRLGRVQFTLAVSLHAPNQTLRELIIP
HHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHCC
SASSYPINSLLKDCKKYIDLTGRRVSFEYILLGGLNDKDIHAEQLANLMRGFQSHVNLIA
CCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEE
YNPIAEENFKRPSQSRVNAFRELLENRGVAVSVRASRGRDKDAACGQLRRQTIDKIKIN
ECCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKLPKLSSNSSLLGLSSEDLEEFARQEGEKSFRGRQIHEWIYQRGAKSLDSISVLPKKW
CCCCCCCCCCCEECCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCHHHHHHCHHHH
RDSLVRKGIQIGRLDEINRVVAEDETLKLLMGTFDGEIVETVGIPTDKRLTVCVSSQIGC
HHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCCCCCEEEEEECCCCCC
PMGCKFCATGKGGLNRSLDVNEIVDQVISVRETMNRRPTHVVFMGMGEPLLNIQNVLDSI
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
ECLTSDIGIGQRKITVSTVGIPNTLSDLAKLAQDRLGRVQFTLAVSLHAPNQTLRELIIP
HHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHCC
SASSYPINSLLKDCKKYIDLTGRRVSFEYILLGGLNDKDIHAEQLANLMRGFQSHVNLIA
CCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEE
YNPIAEENFKRPSQSRVNAFRELLENRGVAVSVRASRGRDKDAACGQLRRQTIDKIKIN
ECCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA