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The map label for this gene is dnaK [H]

Identifier: 124026537

GI number: 124026537

Start: 1493487

End: 1495112

Strand: Direct

Name: dnaK [H]

Synonym: NATL1_18321

Alternate gene names: 124026537

Gene position: 1493487-1495112 (Clockwise)

Preceding gene: 124026533

Following gene: 124026539

Centisome position: 80.09

GC content: 32.9

Gene sequence:

>1626_bases
ATGGAAAAAAATAACATTGATCAATTCGAAAACATCCTTCGACCTGAAATCAATAAAAATGTTTTTGGAACATTAGCCAT
CGATTTAGGAAGTTCAACAACAGTTGTTGTTTTTCAAAAAGAAAATGGGCAGCCTCCTGAACTTTTAGATCTACCTCCCA
TTAGTCGAGCGATTGGAGAGATACCAAGTTTAATTTGGAAGTCATCAGAAAAAGAAGAATCTTATTTTATTGGTCAACAA
ATTATAGATTTAAATCTCATTAATGAAAAAGAAAATAACTTAAGTCAAGATTTTAAAAGATGGATAGGATCTAAGGAAAT
TGAACCTATATATAATTCAAAAATCACCCCAGAAAAAGCAGGTGAGATTTTAATTCACAGTATTTGGGAGAAAGTCTCGC
AGAAGGTCAACATCAAAAGACTAGTATTAACTGCTCCAGTAGATACATATCGAGAATATAGAACTTGGTTAGTCGATGTA
TGTAATTCCTTAGAAGTAAAAGAAATTGCTTTAGTTGATGAACCCACTGCTGCTGCAATGGGAGCTGGACTAGAGCCTGG
ATCAACATTACTTGTTTTAGACTTTGGAGGAAGCACAATTGACATGTCAATTGTTGCTTTAGAGGGAGGAGAAGGACAAG
CTTCACCAATTGCACAGCTTGTAAGATTTGATGGCAATAATTTAGAGGGAAAAAGTACACAAGTTCTTCGCACAGCAAAA
GTTCTAGGTAAATCAGGGCTTCGGTTAGGAGGAAAAGATATAGATAGATGGATATTTCATCATTTATTACCAGAAGAAAA
TCCAACCAATTCAATCTTGCGAAAGGCCGAGGAGCTTAAATGTGAATTAAGCAATACTAATATAAAAGAGACATTGGTTA
TCACTAAAAAAATAAATAATATTCAGAATGAAGAAAAGTTTTTAAAACTATCTAAAAAAGGACTTGAAGAATTACTTATA
GAAAAAGGACTCCTAAAAAGTATTGAAAAGCTTTTCAAACAAACAATTAATAGCGCAAAACGAAATTCATTTGAATTAAA
AGATCTTGATAGCGTTGTCTTAGTCGGAGGAGGGTCTCGCATACCTTTAATTAAAAATTATTTAAGTGATATCTGCAATT
CCATCCCTTTTTTGACCCCTCCACCTATTGAAGCAATTGCATTGGGAGCTTTACACCTCACTCCAGGTGTTCAAATAAAA
GATGTTCTTAACAAAGGGGTAAGTTTAAGATGCTGGAATAAAAAAAATGAAAAGCATATATGGCACCCTCTTTTTCTAGC
AGGTCAGACATGGCCTACAAATAAGCCTTTAGAAATAATTTTAGCTGCAAGTATAAATAGTCAATTAAGCATAGATTTAA
TTATTGGAGAGCCTCAGGAAGAAGGGTCAAATGAGATTATTTATACTAATGGATTACCCACTTTAACAACAATAGAATCC
AAGGATAAAATCAAGAAGATAACTAATACTATTATTTCAATCCCTGTAGATCCTCCTGGTGAAATTGGTCAAGATTGTAT
TAAATTAATTTTTAATATTAATGACAATTGTCAACTTGAGGTTGAGGGTGTCGATTTACGTAATAATAAAGAGATAACAA
AACAGAATCTTGGAGAGATAAGATAA

Upstream 100 bases:

>100_bases
TTCTATATTTTTCACCAAATAATTAAGTGTGATGTTAGTTTTTCTAGACTAACTTGTTACTGATTAATTTTTGTTGCACC
TATAAAAATGCACTAAAAAG

Downstream 100 bases:

>100_bases
ATTTTTTATATTTTTTATCTTAATCATTCATGGTTTTCAAACAAAAAGATACCCTCTCTACCATCAACTTCTCTCAATCT
AAGATCAATTGTCTTATCTT

Product: HSP70 family molecular chaperone

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MEKNNIDQFENILRPEINKNVFGTLAIDLGSSTTVVVFQKENGQPPELLDLPPISRAIGEIPSLIWKSSEKEESYFIGQQ
IIDLNLINEKENNLSQDFKRWIGSKEIEPIYNSKITPEKAGEILIHSIWEKVSQKVNIKRLVLTAPVDTYREYRTWLVDV
CNSLEVKEIALVDEPTAAAMGAGLEPGSTLLVLDFGGSTIDMSIVALEGGEGQASPIAQLVRFDGNNLEGKSTQVLRTAK
VLGKSGLRLGGKDIDRWIFHHLLPEENPTNSILRKAEELKCELSNTNIKETLVITKKINNIQNEEKFLKLSKKGLEELLI
EKGLLKSIEKLFKQTINSAKRNSFELKDLDSVVLVGGGSRIPLIKNYLSDICNSIPFLTPPPIEAIALGALHLTPGVQIK
DVLNKGVSLRCWNKKNEKHIWHPLFLAGQTWPTNKPLEIILAASINSQLSIDLIIGEPQEEGSNEIIYTNGLPTLTTIES
KDKIKKITNTIISIPVDPPGEIGQDCIKLIFNINDNCQLEVEGVDLRNNKEITKQNLGEIR

Sequences:

>Translated_541_residues
MEKNNIDQFENILRPEINKNVFGTLAIDLGSSTTVVVFQKENGQPPELLDLPPISRAIGEIPSLIWKSSEKEESYFIGQQ
IIDLNLINEKENNLSQDFKRWIGSKEIEPIYNSKITPEKAGEILIHSIWEKVSQKVNIKRLVLTAPVDTYREYRTWLVDV
CNSLEVKEIALVDEPTAAAMGAGLEPGSTLLVLDFGGSTIDMSIVALEGGEGQASPIAQLVRFDGNNLEGKSTQVLRTAK
VLGKSGLRLGGKDIDRWIFHHLLPEENPTNSILRKAEELKCELSNTNIKETLVITKKINNIQNEEKFLKLSKKGLEELLI
EKGLLKSIEKLFKQTINSAKRNSFELKDLDSVVLVGGGSRIPLIKNYLSDICNSIPFLTPPPIEAIALGALHLTPGVQIK
DVLNKGVSLRCWNKKNEKHIWHPLFLAGQTWPTNKPLEIILAASINSQLSIDLIIGEPQEEGSNEIIYTNGLPTLTTIES
KDKIKKITNTIISIPVDPPGEIGQDCIKLIFNINDNCQLEVEGVDLRNNKEITKQNLGEIR
>Mature_541_residues
MEKNNIDQFENILRPEINKNVFGTLAIDLGSSTTVVVFQKENGQPPELLDLPPISRAIGEIPSLIWKSSEKEESYFIGQQ
IIDLNLINEKENNLSQDFKRWIGSKEIEPIYNSKITPEKAGEILIHSIWEKVSQKVNIKRLVLTAPVDTYREYRTWLVDV
CNSLEVKEIALVDEPTAAAMGAGLEPGSTLLVLDFGGSTIDMSIVALEGGEGQASPIAQLVRFDGNNLEGKSTQVLRTAK
VLGKSGLRLGGKDIDRWIFHHLLPEENPTNSILRKAEELKCELSNTNIKETLVITKKINNIQNEEKFLKLSKKGLEELLI
EKGLLKSIEKLFKQTINSAKRNSFELKDLDSVVLVGGGSRIPLIKNYLSDICNSIPFLTPPPIEAIALGALHLTPGVQIK
DVLNKGVSLRCWNKKNEKHIWHPLFLAGQTWPTNKPLEIILAASINSQLSIDLIIGEPQEEGSNEIIYTNGLPTLTTIES
KDKIKKITNTIISIPVDPPGEIGQDCIKLIFNINDNCQLEVEGVDLRNNKEITKQNLGEIR

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Homo sapiens, GI24234688, Length=439, Percent_Identity=22.5512528473804, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI34419635, Length=408, Percent_Identity=21.3235294117647, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786196, Length=349, Percent_Identity=26.647564469914, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17507981, Length=429, Percent_Identity=24.4755244755245, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562024, Length=438, Percent_Identity=24.2009132420091, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17534015, Length=427, Percent_Identity=22.7166276346604, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17534013, Length=427, Percent_Identity=22.7166276346604, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI32564617, Length=287, Percent_Identity=24.0418118466899, Blast_Score=66, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322505, Length=439, Percent_Identity=23.6902050113895, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320805, Length=554, Percent_Identity=22.0216606498195, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322426, Length=474, Percent_Identity=23.4177215189873, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24653595, Length=450, Percent_Identity=23.3333333333333, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 60231; Mature: 60231

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNNIDQFENILRPEINKNVFGTLAIDLGSSTTVVVFQKENGQPPELLDLPPISRAIGE
CCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
IPSLIWKSSEKEESYFIGQQIIDLNLINEKENNLSQDFKRWIGSKEIEPIYNSKITPEKA
HHHHHHCCCCCHHHHHHCHHEEEEEEECCCCCCHHHHHHHHCCCCCCCHHHCCCCCHHHH
GEILIHSIWEKVSQKVNIKRLVLTAPVDTYREYRTWLVDVCNSLEVKEIALVDEPTAAAM
HHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHH
GAGLEPGSTLLVLDFGGSTIDMSIVALEGGEGQASPIAQLVRFDGNNLEGKSTQVLRTAK
CCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
VLGKSGLRLGGKDIDRWIFHHLLPEENPTNSILRKAEELKCELSNTNIKETLVITKKINN
HHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCCEEEEEEEHHHHC
IQNEEKFLKLSKKGLEELLIEKGLLKSIEKLFKQTINSAKRNSFELKDLDSVVLVGGGSR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
IPLIKNYLSDICNSIPFLTPPPIEAIALGALHLTPGVQIKDVLNKGVSLRCWNKKNEKHI
CHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEECCCCCCHHHHHCCCCEEEEECCCCCCCC
WHPLFLAGQTWPTNKPLEIILAASINSQLSIDLIIGEPQEEGSNEIIYTNGLPTLTTIES
CCEEEEECCCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCEEEEECCCCCEEEECC
KDKIKKITNTIISIPVDPPGEIGQDCIKLIFNINDNCQLEVEGVDLRNNKEITKQNLGEI
HHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCC
R
C
>Mature Secondary Structure
MEKNNIDQFENILRPEINKNVFGTLAIDLGSSTTVVVFQKENGQPPELLDLPPISRAIGE
CCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
IPSLIWKSSEKEESYFIGQQIIDLNLINEKENNLSQDFKRWIGSKEIEPIYNSKITPEKA
HHHHHHCCCCCHHHHHHCHHEEEEEEECCCCCCHHHHHHHHCCCCCCCHHHCCCCCHHHH
GEILIHSIWEKVSQKVNIKRLVLTAPVDTYREYRTWLVDVCNSLEVKEIALVDEPTAAAM
HHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHH
GAGLEPGSTLLVLDFGGSTIDMSIVALEGGEGQASPIAQLVRFDGNNLEGKSTQVLRTAK
CCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
VLGKSGLRLGGKDIDRWIFHHLLPEENPTNSILRKAEELKCELSNTNIKETLVITKKINN
HHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCCEEEEEEEHHHHC
IQNEEKFLKLSKKGLEELLIEKGLLKSIEKLFKQTINSAKRNSFELKDLDSVVLVGGGSR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
IPLIKNYLSDICNSIPFLTPPPIEAIALGALHLTPGVQIKDVLNKGVSLRCWNKKNEKHI
CHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEECCCCCCHHHHHCCCCEEEEECCCCCCCC
WHPLFLAGQTWPTNKPLEIILAASINSQLSIDLIIGEPQEEGSNEIIYTNGLPTLTTIES
CCEEEEECCCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCEEEEECCCCCEEEECC
KDKIKKITNTIISIPVDPPGEIGQDCIKLIFNINDNCQLEVEGVDLRNNKEITKQNLGEI
HHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA