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The map label for this gene is clpR [H]

Identifier: 124026439

GI number: 124026439

Start: 1420079

End: 1420753

Strand: Direct

Name: clpR [H]

Synonym: NATL1_17341

Alternate gene names: 124026439

Gene position: 1420079-1420753 (Clockwise)

Preceding gene: 124026438

Following gene: 124026440

Centisome position: 76.15

GC content: 41.93

Gene sequence:

>675_bases
ATGACTGCATCATCCCCATATTATGGCGACTCTGCCGTAATGAGAACTCCGCCCCCTGATTTACCATCACTTCTCCTAAA
AGAAAGAATAGTTTATTTAGGCTTGCCATTATTTTCCGATGATGATGCCAAAAGGCAACTCGGAATGGATGTAACTGAAC
TAATTATTGCCCAACTTCTTTTTCTGGAATTTGATAATTCCGAGAAACCTATTTATTTTTATATAAACTCAACTGGAACA
AGCTGGTATACAGGAGATGCAATCGGATTTGAGACTGAGGCCTTTGCAATCTGCGACACCCTCAGATACGTCAAGCCTCC
CGTGCATACTATTTGCATAGGACAAGCGATGGGAACCGCAGCAGTAATACTCTCAGCAGGAACAAAAGGGCAACGTGCAG
CATTACCTCATGCATCTATCGTCCTTCATCAGCCTAGAAGTGGGGCTCAAGGCCAAGCAACTGATATTCAAATCAGGGCG
AATGAGGTTATTCACAATAAGAAAGCCATGCTAGAAATACTTAGTCATAACACTGGAAGATCAGTAGATCAATTATCCAA
AGACTCGGATCGAATGAGTTATTTGAACCCTCATGAAGCAGTTGATTATGGGATTATTGATCGCGTTCTTACTAGTAGAA
AAGACTTGCCAGGCGAGAAAGTTTTGCCCACATAA

Upstream 100 bases:

>100_bases
GTAAAGCTTCAAATGATCAGCCCCCGAACAAATTTGAAAAAACAAAAGCATCTCAAGGCTAGATCTAGCTAGGCTCGAAT
CCAAGAAGACTTAACTTCAT

Downstream 100 bases:

>100_bases
CCAAATTATTTTTACGAATTTTCAAGTCATTACCCTCAACTACTAAACATCAAATTAGCCATGCCAATAGGTACTCCAAG
CGTTCCATACCGTCTTCCAG

Product: ATP-dependent Clp protease-like protein

Products: NA

Alternate protein names: Endopeptidase Clp-like [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTASSPYYGDSAVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLLFLEFDNSEKPIYFYINSTGT
SWYTGDAIGFETEAFAICDTLRYVKPPVHTICIGQAMGTAAVILSAGTKGQRAALPHASIVLHQPRSGAQGQATDIQIRA
NEVIHNKKAMLEILSHNTGRSVDQLSKDSDRMSYLNPHEAVDYGIIDRVLTSRKDLPGEKVLPT

Sequences:

>Translated_224_residues
MTASSPYYGDSAVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLLFLEFDNSEKPIYFYINSTGT
SWYTGDAIGFETEAFAICDTLRYVKPPVHTICIGQAMGTAAVILSAGTKGQRAALPHASIVLHQPRSGAQGQATDIQIRA
NEVIHNKKAMLEILSHNTGRSVDQLSKDSDRMSYLNPHEAVDYGIIDRVLTSRKDLPGEKVLPT
>Mature_223_residues
TASSPYYGDSAVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLLFLEFDNSEKPIYFYINSTGTS
WYTGDAIGFETEAFAICDTLRYVKPPVHTICIGQAMGTAAVILSAGTKGQRAALPHASIVLHQPRSGAQGQATDIQIRAN
EVIHNKKAMLEILSHNTGRSVDQLSKDSDRMSYLNPHEAVDYGIIDRVLTSRKDLPGEKVLPT

Specific function: Has lost the two conserved residues (Ser and His) proposed to be part of the active site. Therefore it could be inactive [H]

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family [H]

Homologues:

Organism=Homo sapiens, GI5174419, Length=192, Percent_Identity=41.1458333333333, Blast_Score=145, Evalue=2e-35,
Organism=Escherichia coli, GI1786641, Length=195, Percent_Identity=42.5641025641026, Blast_Score=162, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17538017, Length=200, Percent_Identity=40, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI20129427, Length=191, Percent_Identity=41.8848167539267, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001907 [H]

Pfam domain/function: PF00574 CLP_protease [H]

EC number: 3.4.21.92

Molecular weight: Translated: 24621; Mature: 24490

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASSPYYGDSAVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLL
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHH
FLEFDNSEKPIYFYINSTGTSWYTGDAIGFETEAFAICDTLRYVKPPVHTICIGQAMGTA
HHEECCCCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHEEEECHHHCCE
AVILSAGTKGQRAALPHASIVLHQPRSGAQGQATDIQIRANEVIHNKKAMLEILSHNTGR
EEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCC
SVDQLSKDSDRMSYLNPHEAVDYGIIDRVLTSRKDLPGEKVLPT
CHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TASSPYYGDSAVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLL
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHH
FLEFDNSEKPIYFYINSTGTSWYTGDAIGFETEAFAICDTLRYVKPPVHTICIGQAMGTA
HHEECCCCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHEEEECHHHCCE
AVILSAGTKGQRAALPHASIVLHQPRSGAQGQATDIQIRANEVIHNKKAMLEILSHNTGR
EEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCC
SVDQLSKDSDRMSYLNPHEAVDYGIIDRVLTSRKDLPGEKVLPT
CHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP imidodiphosphate; dATP degradation, [C]

Metal ions: Ca2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 800 [C]

Specific activity: NA

Km value (mM): 0.21 {ATP}} 1.3 {N-succinyl-Leu-Tyr} [C]

Substrates: NA

Specific reaction: NA

General reaction: Hydrolysis of peptide bonds [C]

Inhibitor: Diisopropyl fluorophosphate ClpAP; Fluorosulfonyl benzoyl adenosine; Kappa-Casein; Mg2+; NEM hydrolysis; Neohydrin; Succinyl -Leu-Tyr4-methyl coumarin7-amide; Xaa-Tyr-Leu-Tyr-Trp [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]