Click here to switch to the map view.

The map label for this gene is trmD [H]

Identifier: 124026405

GI number: 124026405

Start: 1384402

End: 1385628

Strand: Reverse

Name: trmD [H]

Synonym: NATL1_17001

Alternate gene names: 124026405

Gene position: 1385628-1384402 (Counterclockwise)

Preceding gene: 124026406

Following gene: 124026404

Centisome position: 74.31

GC content: 36.51

Gene sequence:

>1227_bases
ATGACTAAATTAAGATTCGATGTCGTAAGCCTTTTTCCAGAAGCTTTTAAAAATTTTTTTAATCATGGACTAATAAAAAA
AGCATTTGAAGAGAAGATTGCATCAATTCATATACATAATCCTCGTGACCACGCTATGGATAATTATCGAAAAGTTGATG
ATGAGCCATATGGCGGAGGCGCAGGAATGGTCTTAAAGCCAGAACCCTACTTTTCGGTTTTTGATCAAATTCCAAAGCTC
AATAAAAAAAGAATACTTTTGATGACTCCACAAGGTAGAAAAATATCTCAACCTGATTTTTCTAGATGGTCAAAAGAAGA
TCAACTCATATTGATATGTGGTAGCTATGAGGGATTTGATGAAAGGATTAGGTCTTTAGCTGATGAAGAAATTTCAATTG
GAGACTTTGTTCTTACGGGTGGAGAAATTCCTGCAATAACTCTTATTAATGGAGTTGTACGTCTATTACCGGGAACTTTA
GGCAGTGCCGAATCATTAGAAGAAGAAAGTCATAATGAGTTTCTTTTAGAACATCCCCAATACACCCGACCTGCAGAATT
TAGAGGTGTGAAAGTTCCTGATGTCCTTTTAAGTGGTAATCATAAATTAATTAGAGAATGGAGGCAGAAGCAAAGAGAAA
TCAGGACGAAGTCACGTAGACCCGATTTGTTCGAACTTTGGAAGCTCGACCAATTATCATTTATTAAAAGAAGTTCATTA
TTGAAAACTGAAGTGAACTTACGAATAGGCAACGGTTATGACATGCACCGATTGGTTTCTGGACGACCTTTAATTCTTGG
AGGGGTCGAGTTAAACCATCCAGAGGGTTTAGGCCTTGATGGGCATAGTGATGCAGATGTTCTTACTCATGCAATTATGG
ACGCAATATTAGGTGCCTTATCATTGGGTGATATTGGAAAGTATTTCCCTCCAGATGACCCTAAGTGGAAAAATGCAGAC
AGTTTAATTCTGCTTGGACATGTCATGGAATTAATTGAGAAGAAAGGTTGGCAAATTCAAAATATTGATTCAGTTATTGT
CGCTGAAAGGCCAAAACTAAAACCTTATATTGACTTAATGAAGGAGAAGATATCTAAGAAAATAGGATTGAATATTGATG
ATGTAGGAGTTAAAGCTACTACAAATGAGAAATTAGGTGCGGAGGGAAGAGAAGAGGGTATATGTTGTCATGCAGTTGTT
TTAATGAAAAGGAATGAAAACTCTTAG

Upstream 100 bases:

>100_bases
ATGGAAATGTCTATCTAGAACTGTTTGTTAAAGTTGTGCCTGACTGGAGAAGTAAATCTTCTCGGCTTAATGAGTTTGGT
TATGAAGGTAGCTAATTTAT

Downstream 100 bases:

>100_bases
AACTAAATATTTTATCGAAAAAATATTTAGTTTCCTTTTATTAGGTGTGATTTTTATTTCAACTATTTTTGGAAACGTGA
AAAATATTCAAGCTGCGTCT

Product: putative bifuntional enzyme: tRNA methyltransferase; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MTKLRFDVVSLFPEAFKNFFNHGLIKKAFEEKIASIHIHNPRDHAMDNYRKVDDEPYGGGAGMVLKPEPYFSVFDQIPKL
NKKRILLMTPQGRKISQPDFSRWSKEDQLILICGSYEGFDERIRSLADEEISIGDFVLTGGEIPAITLINGVVRLLPGTL
GSAESLEEESHNEFLLEHPQYTRPAEFRGVKVPDVLLSGNHKLIREWRQKQREIRTKSRRPDLFELWKLDQLSFIKRSSL
LKTEVNLRIGNGYDMHRLVSGRPLILGGVELNHPEGLGLDGHSDADVLTHAIMDAILGALSLGDIGKYFPPDDPKWKNAD
SLILLGHVMELIEKKGWQIQNIDSVIVAERPKLKPYIDLMKEKISKKIGLNIDDVGVKATTNEKLGAEGREEGICCHAVV
LMKRNENS

Sequences:

>Translated_408_residues
MTKLRFDVVSLFPEAFKNFFNHGLIKKAFEEKIASIHIHNPRDHAMDNYRKVDDEPYGGGAGMVLKPEPYFSVFDQIPKL
NKKRILLMTPQGRKISQPDFSRWSKEDQLILICGSYEGFDERIRSLADEEISIGDFVLTGGEIPAITLINGVVRLLPGTL
GSAESLEEESHNEFLLEHPQYTRPAEFRGVKVPDVLLSGNHKLIREWRQKQREIRTKSRRPDLFELWKLDQLSFIKRSSL
LKTEVNLRIGNGYDMHRLVSGRPLILGGVELNHPEGLGLDGHSDADVLTHAIMDAILGALSLGDIGKYFPPDDPKWKNAD
SLILLGHVMELIEKKGWQIQNIDSVIVAERPKLKPYIDLMKEKISKKIGLNIDDVGVKATTNEKLGAEGREEGICCHAVV
LMKRNENS
>Mature_407_residues
TKLRFDVVSLFPEAFKNFFNHGLIKKAFEEKIASIHIHNPRDHAMDNYRKVDDEPYGGGAGMVLKPEPYFSVFDQIPKLN
KKRILLMTPQGRKISQPDFSRWSKEDQLILICGSYEGFDERIRSLADEEISIGDFVLTGGEIPAITLINGVVRLLPGTLG
SAESLEEESHNEFLLEHPQYTRPAEFRGVKVPDVLLSGNHKLIREWRQKQREIRTKSRRPDLFELWKLDQLSFIKRSSLL
KTEVNLRIGNGYDMHRLVSGRPLILGGVELNHPEGLGLDGHSDADVLTHAIMDAILGALSLGDIGKYFPPDDPKWKNADS
LILLGHVMELIEKKGWQIQNIDSVIVAERPKLKPYIDLMKEKISKKIGLNIDDVGVKATTNEKLGAEGREEGICCHAVVL
MKRNENS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=221, Percent_Identity=46.1538461538462, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=49.3589743589744, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 46145; Mature: 46014

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLRFDVVSLFPEAFKNFFNHGLIKKAFEEKIASIHIHNPRDHAMDNYRKVDDEPYGGG
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCCCCC
AGMVLKPEPYFSVFDQIPKLNKKRILLMTPQGRKISQPDFSRWSKEDQLILICGSYEGFD
CCEEECCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCCEEEEECCCCCHH
ERIRSLADEEISIGDFVLTGGEIPAITLINGVVRLLPGTLGSAESLEEESHNEFLLEHPQ
HHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEECCCC
YTRPAEFRGVKVPDVLLSGNHKLIREWRQKQREIRTKSRRPDLFELWKLDQLSFIKRSSL
CCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LKTEVNLRIGNGYDMHRLVSGRPLILGGVELNHPEGLGLDGHSDADVLTHAIMDAILGAL
HEEEEEEEECCCCCHHHHHCCCCEEEECEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SLGDIGKYFPPDDPKWKNADSLILLGHVMELIEKKGWQIQNIDSVIVAERPKLKPYIDLM
HHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCEEECCCCEEEECCCCCCHHHHHH
KEKISKKIGLNIDDVGVKATTNEKLGAEGREEGICCHAVVLMKRNENS
HHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCEEEEEEEEEECCCCC
>Mature Secondary Structure 
TKLRFDVVSLFPEAFKNFFNHGLIKKAFEEKIASIHIHNPRDHAMDNYRKVDDEPYGGG
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCCCCC
AGMVLKPEPYFSVFDQIPKLNKKRILLMTPQGRKISQPDFSRWSKEDQLILICGSYEGFD
CCEEECCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCCEEEEECCCCCHH
ERIRSLADEEISIGDFVLTGGEIPAITLINGVVRLLPGTLGSAESLEEESHNEFLLEHPQ
HHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEECCCC
YTRPAEFRGVKVPDVLLSGNHKLIREWRQKQREIRTKSRRPDLFELWKLDQLSFIKRSSL
CCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LKTEVNLRIGNGYDMHRLVSGRPLILGGVELNHPEGLGLDGHSDADVLTHAIMDAILGAL
HEEEEEEEECCCCCHHHHHCCCCEEEECEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SLGDIGKYFPPDDPKWKNADSLILLGHVMELIEKKGWQIQNIDSVIVAERPKLKPYIDLM
HHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCEEECCCCEEEECCCCCCHHHHHH
KEKISKKIGLNIDDVGVKATTNEKLGAEGREEGICCHAVVLMKRNENS
HHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA