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The map label for this gene is pnp

Identifier: 124026371

GI number: 124026371

Start: 1356705

End: 1358873

Strand: Direct

Name: pnp

Synonym: NATL1_16651

Alternate gene names: 124026371

Gene position: 1356705-1358873 (Clockwise)

Preceding gene: 124026370

Following gene: 124026373

Centisome position: 72.76

GC content: 40.89

Gene sequence:

>2169_bases
GTGCAAGGTCAGACAAAATCCGTTTCCTTCGATGGTAGAGAGATAAAGCTCACTACAGGGAGATTTGCTCCACAAGCTGG
TGGTTCAGTAATGATTGAGTGTGGGGACACCTCTGTTCTAGTAACAGCAACAAAATCGTCAGGAAGAGAGGGGGTTGATT
TCTTACCCTTAATGTGTGATTACGAAGAAAGGCTATATGCGGCAGGAAGGATTCCAGGGAGTTTCATGCGTCGTGAAGGG
CGTCCTCCTGAGAGAGCAACTTTAATTTCACGACTCATTGATCGTCCAATGAGACCTCTTTTCCCAGGTTGGATGAGAGA
CGATATCCAAATAGTTGCTACATGCCTCTCCTTAGATGAGAGAGTTCCAGCGGATGTTTTGGCTGTTACGGGAGCTTCAA
TGGCAACTTTAATGGCAGGTATTCCTTTCCAAGGACCTATGGCTGCTGTTCGCGTTGGGCTCTTGGGAGATGACTTTGTC
CTGAATCCTAGTTATCGAGAAATAGAAAGAGGTGATCTAGACCTTGTAGTTGCTGGAACCCCAGATGGTGTAGTAATGGT
TGAAGCAGGAGCCAATCAACTTTCAGAACAAGACGTTATTGAAGCCATTGATTTTGGCTACGAGGCCATAACTGAATTAA
TCAATGCTCAAAAAGAAGTTTTAAAAGAATCTGGAATTAAGCAAGAGATGCCTAAAGCCCCTGAGATTGATGACACAATA
TCAACCTATTTAGATAAAAACTGCACAAAATCAATTAGTGAAGTTCTAAAGAACTTTGATCAAACAAAGGAAGAGAGAGA
CAATAAAATTGAAGAAATAAAGATAAGTATTTCTGCAAAAATAGATGGACTAAAAGATGATAATGCTGTAAAAAAATCTC
TTTCTTTAAATAACAAGCTCCTAGAAAATAGTTATAAAGCTTTAACCAAAAAGTTAATGAGAGAGCAGATAATCAAAGAA
GGGAAAAGAGTCGATGGAAGAGAATTAAATGAAGTAAGAGCTATTGAGGCTGATGCTGCAGTTTTACCCAACAGAGTTCA
TGGATCAGCGTTATTCCAAAGAGGTTTAACTCAAGTCCTCTCCACTGCAACATTGGGCACTCCAAGCGATGCTCAGGAAA
TGGACGATTTAAACCCTAATACTGATAAAACCTACATACATCATTACAACTTCCCACCTTACTCAGTGGGAGAGACTCGA
CCAATGCGGACTCCTGGAAGAAGAGAAGTTGGTCATGGAGCTTTAGCAGAAAGGGCGTTAATACCCGTACTACCAGCAAA
AGATACTTTCCCATATGTTTTAAGAGTTGTTAGCGAAGTCTTGAGTTCAAACGGCTCTACCTCTATGGCTTCAGTATGTG
GAAGTACACTTGCACTTATGGATGCAGGAGTCCCATTAAAAGCCCCTGTAGGAGGAGCAGCTATGGGTTTAATCAAAGAA
GGAAAGGAAGTACGCATATTGACTGATATTCAAGGCATTGAAGACTTTCTTGGAGACATGGATTTCAAAGTTGCAGGGAC
AGAAAAAGGAATTACAGCCTTGCAAATGGATATGAAAATAACTGGACTTCCGATTGAGACAATTGGGGAAGCTATTAATC
AAGCACTTCCTGCGAGAACACACATATTAGGAAAAATGCTAGATGCAATTGAGACACCAAAAGACAACTTATCTCCTCAT
GCCCCAAGACTGTTAAGCTTTAGAATCGATCCAGAGCTCATAGGAACTGTCATTGGCCCTGGAGGAAGAACAATAAAGGG
AATCACAGAGAGAACTAACACAAAAATCGATATAGAAGATGGAGGGATTGTCACTATTGCATCTCATGACGGAGCAGCTG
CAGAAGAAGCGCAGAGGATTATTGAAGGTTTAACGAGAAAAGTACATGAGGGAGAAATTTTCCCTGGCTCTATCACAAGA
ATCATTCCAATAGGCGCCTTTGTTGAAATCCTTCCTGGTAAAGAAGGAATGATACATATTTCTCAATTATCCGAAGCAAG
AGTTGAAAAAGTTGAAGATGTTGTAAAAGTTGGAGACCAAGTGACTGTAAGGGTAAGAGAAATAGATAATCGTGGACGTA
TTAATTTAACTTTGAGAGGAGTCTCTCAAAATGGTGGAATGAGCAATTACCCTGAGCCAACTCCTACTCCTGTTGCCCCT
CTTACTTAA

Upstream 100 bases:

>100_bases
GTTGTAAATGTTTCCTCTAGAAAACAAATCTATCGAAATTTTTACTCAATTAGTCCCATTAAGTGTAAGAATGTCTGAAG
ACAAAAAGCTATAAAGGCAC

Downstream 100 bases:

>100_bases
TAATTAGACGAAGTATTTAGGTTCTTCCAGAGAGAAAAAATCCATAGCTTTTTGGCAAATCTTTTGATGAGATTTCCCAT
GGCTTGCAATTAAACAACCA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MQGQTKSVSFDGREIKLTTGRFAPQAGGSVMIECGDTSVLVTATKSSGREGVDFLPLMCDYEERLYAAGRIPGSFMRREG
RPPERATLISRLIDRPMRPLFPGWMRDDIQIVATCLSLDERVPADVLAVTGASMATLMAGIPFQGPMAAVRVGLLGDDFV
LNPSYREIERGDLDLVVAGTPDGVVMVEAGANQLSEQDVIEAIDFGYEAITELINAQKEVLKESGIKQEMPKAPEIDDTI
STYLDKNCTKSISEVLKNFDQTKEERDNKIEEIKISISAKIDGLKDDNAVKKSLSLNNKLLENSYKALTKKLMREQIIKE
GKRVDGRELNEVRAIEADAAVLPNRVHGSALFQRGLTQVLSTATLGTPSDAQEMDDLNPNTDKTYIHHYNFPPYSVGETR
PMRTPGRREVGHGALAERALIPVLPAKDTFPYVLRVVSEVLSSNGSTSMASVCGSTLALMDAGVPLKAPVGGAAMGLIKE
GKEVRILTDIQGIEDFLGDMDFKVAGTEKGITALQMDMKITGLPIETIGEAINQALPARTHILGKMLDAIETPKDNLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEEAQRIIEGLTRKVHEGEIFPGSITR
IIPIGAFVEILPGKEGMIHISQLSEARVEKVEDVVKVGDQVTVRVREIDNRGRINLTLRGVSQNGGMSNYPEPTPTPVAP
LT

Sequences:

>Translated_722_residues
MQGQTKSVSFDGREIKLTTGRFAPQAGGSVMIECGDTSVLVTATKSSGREGVDFLPLMCDYEERLYAAGRIPGSFMRREG
RPPERATLISRLIDRPMRPLFPGWMRDDIQIVATCLSLDERVPADVLAVTGASMATLMAGIPFQGPMAAVRVGLLGDDFV
LNPSYREIERGDLDLVVAGTPDGVVMVEAGANQLSEQDVIEAIDFGYEAITELINAQKEVLKESGIKQEMPKAPEIDDTI
STYLDKNCTKSISEVLKNFDQTKEERDNKIEEIKISISAKIDGLKDDNAVKKSLSLNNKLLENSYKALTKKLMREQIIKE
GKRVDGRELNEVRAIEADAAVLPNRVHGSALFQRGLTQVLSTATLGTPSDAQEMDDLNPNTDKTYIHHYNFPPYSVGETR
PMRTPGRREVGHGALAERALIPVLPAKDTFPYVLRVVSEVLSSNGSTSMASVCGSTLALMDAGVPLKAPVGGAAMGLIKE
GKEVRILTDIQGIEDFLGDMDFKVAGTEKGITALQMDMKITGLPIETIGEAINQALPARTHILGKMLDAIETPKDNLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEEAQRIIEGLTRKVHEGEIFPGSITR
IIPIGAFVEILPGKEGMIHISQLSEARVEKVEDVVKVGDQVTVRVREIDNRGRINLTLRGVSQNGGMSNYPEPTPTPVAP
LT
>Mature_722_residues
MQGQTKSVSFDGREIKLTTGRFAPQAGGSVMIECGDTSVLVTATKSSGREGVDFLPLMCDYEERLYAAGRIPGSFMRREG
RPPERATLISRLIDRPMRPLFPGWMRDDIQIVATCLSLDERVPADVLAVTGASMATLMAGIPFQGPMAAVRVGLLGDDFV
LNPSYREIERGDLDLVVAGTPDGVVMVEAGANQLSEQDVIEAIDFGYEAITELINAQKEVLKESGIKQEMPKAPEIDDTI
STYLDKNCTKSISEVLKNFDQTKEERDNKIEEIKISISAKIDGLKDDNAVKKSLSLNNKLLENSYKALTKKLMREQIIKE
GKRVDGRELNEVRAIEADAAVLPNRVHGSALFQRGLTQVLSTATLGTPSDAQEMDDLNPNTDKTYIHHYNFPPYSVGETR
PMRTPGRREVGHGALAERALIPVLPAKDTFPYVLRVVSEVLSSNGSTSMASVCGSTLALMDAGVPLKAPVGGAAMGLIKE
GKEVRILTDIQGIEDFLGDMDFKVAGTEKGITALQMDMKITGLPIETIGEAINQALPARTHILGKMLDAIETPKDNLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEEAQRIIEGLTRKVHEGEIFPGSITR
IIPIGAFVEILPGKEGMIHISQLSEARVEKVEDVVKVGDQVTVRVREIDNRGRINLTLRGVSQNGGMSNYPEPTPTPVAP
LT

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=703, Percent_Identity=35.8463726884779, Blast_Score=430, Evalue=1e-120,
Organism=Escherichia coli, GI145693187, Length=701, Percent_Identity=46.6476462196862, Blast_Score=612, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI115534063, Length=701, Percent_Identity=34.2368045649073, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI281362905, Length=701, Percent_Identity=37.3751783166904, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651641, Length=701, Percent_Identity=37.3751783166904, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651643, Length=701, Percent_Identity=37.3751783166904, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI161079377, Length=641, Percent_Identity=37.1294851794072, Blast_Score=404, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_PROM1 (A2C412)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015487.1
- ProteinModelPortal:   A2C412
- STRING:   A2C412
- GeneID:   4779070
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_16651
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   PMAR167555:NATL1_16651-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 78274; Mature: 78274

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGQTKSVSFDGREIKLTTGRFAPQAGGSVMIECGDTSVLVTATKSSGREGVDFLPLMCD
CCCCCCCEECCCCEEEEECCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCHHEEC
YEERLYAAGRIPGSFMRREGRPPERATLISRLIDRPMRPLFPGWMRDDIQIVATCLSLDE
HHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
RVPADVLAVTGASMATLMAGIPFQGPMAAVRVGLLGDDFVLNPSYREIERGDLDLVVAGT
CCCHHHHHHCCHHHHHHHHCCCCCCCHHHHEEEECCCCEEECCCHHHHCCCCEEEEEECC
PDGVVMVEAGANQLSEQDVIEAIDFGYEAITELINAQKEVLKESGIKQEMPKAPEIDDTI
CCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHH
STYLDKNCTKSISEVLKNFDQTKEERDNKIEEIKISISAKIDGLKDDNAVKKSLSLNNKL
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHH
LENSYKALTKKLMREQIIKEGKRVDGRELNEVRAIEADAAVLPNRVHGSALFQRGLTQVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHH
STATLGTPSDAQEMDDLNPNTDKTYIHHYNFPPYSVGETRPMRTPGRREVGHGALAERAL
HHHCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHCCCCHHHHCCC
IPVLPAKDTFPYVLRVVSEVLSSNGSTSMASVCGSTLALMDAGVPLKAPVGGAAMGLIKE
EECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
GKEVRILTDIQGIEDFLGDMDFKVAGTEKGITALQMDMKITGLPIETIGEAINQALPART
CCEEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCHHH
HILGKMLDAIETPKDNLSPHAPRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIED
HHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHEEECCCCCEEECHHHCCCCEEEECC
GGIVTIASHDGAAAEEAQRIIEGLTRKVHEGEIFPGSITRIIPIGAFVEILPGKEGMIHI
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEHHHHHEECCCCCCEEEH
SQLSEARVEKVEDVVKVGDQVTVRVREIDNRGRINLTLRGVSQNGGMSNYPEPTPTPVAP
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCC
LT
CC
>Mature Secondary Structure
MQGQTKSVSFDGREIKLTTGRFAPQAGGSVMIECGDTSVLVTATKSSGREGVDFLPLMCD
CCCCCCCEECCCCEEEEECCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCHHEEC
YEERLYAAGRIPGSFMRREGRPPERATLISRLIDRPMRPLFPGWMRDDIQIVATCLSLDE
HHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
RVPADVLAVTGASMATLMAGIPFQGPMAAVRVGLLGDDFVLNPSYREIERGDLDLVVAGT
CCCHHHHHHCCHHHHHHHHCCCCCCCHHHHEEEECCCCEEECCCHHHHCCCCEEEEEECC
PDGVVMVEAGANQLSEQDVIEAIDFGYEAITELINAQKEVLKESGIKQEMPKAPEIDDTI
CCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHH
STYLDKNCTKSISEVLKNFDQTKEERDNKIEEIKISISAKIDGLKDDNAVKKSLSLNNKL
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHH
LENSYKALTKKLMREQIIKEGKRVDGRELNEVRAIEADAAVLPNRVHGSALFQRGLTQVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHH
STATLGTPSDAQEMDDLNPNTDKTYIHHYNFPPYSVGETRPMRTPGRREVGHGALAERAL
HHHCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHCCCCHHHHCCC
IPVLPAKDTFPYVLRVVSEVLSSNGSTSMASVCGSTLALMDAGVPLKAPVGGAAMGLIKE
EECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
GKEVRILTDIQGIEDFLGDMDFKVAGTEKGITALQMDMKITGLPIETIGEAINQALPART
CCEEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCHHH
HILGKMLDAIETPKDNLSPHAPRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIED
HHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHEEECCCCCEEECHHHCCCCEEEECC
GGIVTIASHDGAAAEEAQRIIEGLTRKVHEGEIFPGSITRIIPIGAFVEILPGKEGMIHI
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEHHHHHEECCCCCCEEEH
SQLSEARVEKVEDVVKVGDQVTVRVREIDNRGRINLTLRGVSQNGGMSNYPEPTPTPVAP
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCC
LT
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA