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The map label for this gene is sppA [C]

Identifier: 124026360

GI number: 124026360

Start: 1347942

End: 1348751

Strand: Direct

Name: sppA [C]

Synonym: NATL1_16541

Alternate gene names: 124026360

Gene position: 1347942-1348751 (Clockwise)

Preceding gene: 124026356

Following gene: 124026361

Centisome position: 72.29

GC content: 37.41

Gene sequence:

>810_bases
ATGATTTGGCCCTTGAGACGAAAATCCAAAAAGAGGATGGCACGAATCTGTATTGAAGGTCCTATCAATTCAGAAACTCG
GAAAATTGTTCTAAAAGCATTAAAGCAAATTGAGGAAAGAGAGTTTCCAGCACTATTACTTCGCATTGATAGCCCTGGGG
GAACAGTTGGTGATAGCCAAGAAATCCATAGTGCTCTCTTGAGGCTAAGAGAGAAAGGTTGTCATGTTGTAGCTAGTTTT
GGCAATATCTCAGCTTCAGGTGGAGTCTATATAGGTGTAGGTGCTGAAAAAATTGTTGCAAACCCAGGAACAATAACAGG
ATCTATTGGTGTAATTTTAAGAGGAAACAATCTATCAAAGTTATTAGAAAAGGTTGGTATTAAATTCGAGACTGTAAAAA
GTGGAATCTATAAAGACATTCTTTCCCCTGATCGCCCTTTATCAACTGAGGAGAGAGCTCTTCTACAATCTTTAATTGAT
AGCAGTTACGAGCAATTTGTTTTAGCAGTTTCAAAAGGAAGAAATTTAACCCCAGAAGTGGTTAAGAGTTTTGCCGATGG
AAGAGTTTTTACTGGAGAGCAAGCTAAAGAATTTGGGTTGGTAGATGAAATAGGTGATGAGAATGATGCAAAACTACTTG
CTATAAAAATTGCGAACCTAGATGAAAAAACAAAACCCATAACATTTGGTAAAACCAAAAAGAAATTATTAGGGTTTTTA
CCTGGAGGGAAAATAATCCACAATCTTGCAAATGCATTAAACCTTGAGTTGGAGGGGAATGGACAGATCCTTTGGCTCTT
TAAGCCATGA

Upstream 100 bases:

>100_bases
AGAGCGAAGGGCAAAATCATCAAAAACCAATTCCAAATGACAAACATAAGGTCAGAAAGCAATCTCCTTTTTAAGATCAA
TAAAGTTATGCATAAATTGA

Downstream 100 bases:

>100_bases
ATTGTCAAAATGAATTGACTTATCTGTGTGCATTGAGGGGGGCAACGACTTGTGAAAACAATTCTGTTGAGTCAATCACT
TCTGCGGTAGAGGAATTGCT

Product: signal peptide peptidase SppA (protease IV)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MIWPLRRKSKKRMARICIEGPINSETRKIVLKALKQIEEREFPALLLRIDSPGGTVGDSQEIHSALLRLREKGCHVVASF
GNISASGGVYIGVGAEKIVANPGTITGSIGVILRGNNLSKLLEKVGIKFETVKSGIYKDILSPDRPLSTEERALLQSLID
SSYEQFVLAVSKGRNLTPEVVKSFADGRVFTGEQAKEFGLVDEIGDENDAKLLAIKIANLDEKTKPITFGKTKKKLLGFL
PGGKIIHNLANALNLELEGNGQILWLFKP

Sequences:

>Translated_269_residues
MIWPLRRKSKKRMARICIEGPINSETRKIVLKALKQIEEREFPALLLRIDSPGGTVGDSQEIHSALLRLREKGCHVVASF
GNISASGGVYIGVGAEKIVANPGTITGSIGVILRGNNLSKLLEKVGIKFETVKSGIYKDILSPDRPLSTEERALLQSLID
SSYEQFVLAVSKGRNLTPEVVKSFADGRVFTGEQAKEFGLVDEIGDENDAKLLAIKIANLDEKTKPITFGKTKKKLLGFL
PGGKIIHNLANALNLELEGNGQILWLFKP
>Mature_269_residues
MIWPLRRKSKKRMARICIEGPINSETRKIVLKALKQIEEREFPALLLRIDSPGGTVGDSQEIHSALLRLREKGCHVVASF
GNISASGGVYIGVGAEKIVANPGTITGSIGVILRGNNLSKLLEKVGIKFETVKSGIYKDILSPDRPLSTEERALLQSLID
SSYEQFVLAVSKGRNLTPEVVKSFADGRVFTGEQAKEFGLVDEIGDENDAKLLAIKIANLDEKTKPITFGKTKKKLLGFL
PGGKIIHNLANALNLELEGNGQILWLFKP

Specific function: Digestion Of The Cleaved Signal Peptides. This Activity Is Necessary To Maintain Proper Secretion Of Mature Proteins Across The Membrane. [C]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=201, Percent_Identity=35.8208955223881, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787527, Length=169, Percent_Identity=32.5443786982249, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 29398; Mature: 29398

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWPLRRKSKKRMARICIEGPINSETRKIVLKALKQIEEREFPALLLRIDSPGGTVGDSQ
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHH
EIHSALLRLREKGCHVVASFGNISASGGVYIGVGAEKIVANPGTITGSIGVILRGNNLSK
HHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHEECCCCEEEECEEEEEECCCHHH
LLEKVGIKFETVKSGIYKDILSPDRPLSTEERALLQSLIDSSYEQFVLAVSKGRNLTPEV
HHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHEEHHHCCCCCCCHHH
VKSFADGRVFTGEQAKEFGLVDEIGDENDAKLLAIKIANLDEKTKPITFGKTKKKLLGFL
HHHHCCCCEECCCCHHHCCCHHHCCCCCCCEEEEEEEECCCCCCCCEECCCCHHHHHHCC
PGGKIIHNLANALNLELEGNGQILWLFKP
CCHHHHHHHHHHEEEEECCCCEEEEEECC
>Mature Secondary Structure
MIWPLRRKSKKRMARICIEGPINSETRKIVLKALKQIEEREFPALLLRIDSPGGTVGDSQ
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHH
EIHSALLRLREKGCHVVASFGNISASGGVYIGVGAEKIVANPGTITGSIGVILRGNNLSK
HHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHEECCCCEEEECEEEEEECCCHHH
LLEKVGIKFETVKSGIYKDILSPDRPLSTEERALLQSLIDSSYEQFVLAVSKGRNLTPEV
HHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHEEHHHCCCCCCCHHH
VKSFADGRVFTGEQAKEFGLVDEIGDENDAKLLAIKIANLDEKTKPITFGKTKKKLLGFL
HHHHCCCCEECCCCHHHCCCHHHCCCCCCCEEEEEEEECCCCCCCCEECCCCHHHHHHCC
PGGKIIHNLANALNLELEGNGQILWLFKP
CCHHHHHHHHHHEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]