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The map label for this gene is lipA2 [H]

Identifier: 124026190

GI number: 124026190

Start: 1194926

End: 1195849

Strand: Direct

Name: lipA2 [H]

Synonym: NATL1_14841

Alternate gene names: 124026190

Gene position: 1194926-1195849 (Clockwise)

Preceding gene: 124026189

Following gene: 124026197

Centisome position: 64.08

GC content: 39.72

Gene sequence:

>924_bases
ATGACAACAACAACAAGAAAAAAAACAAAATACAGTAGCTTCCTCCCAGAAGAGCGTTTACCTAATTGGATCAAACCATC
CATAGGTAACGCAACTCAACTAGAGAAAGTTCAAAAGCTCGTAAAGGAAAACAGATTACATACGATCTGTGAAGAAGGCA
GATGCCCGAACAGAGGTGAATGTTATGCCGCTGGTACGGCTACTTTTTTATTGGGCGGCTCAATATGTACAAGGAGCTGT
GCCTTTTGCCAAGTAGAGAAAGGAAAGTCGCCAGAGAAAGTAAATATTTTTGAGGCAGAGAAAGTAGCAAATGCTGTTCA
GGTATTGAATCTTAAATATGTGGTACTAACAGCAGTCGCAAGAGATGATTTGCCGGATCACGGTGCAAGTCTATTCACGA
CAACAATGGATGCAATTAGATCCTTGAATCCAGGTGTCGCAATAGAGGTACTAACACCAGATTTCTGGGGTGGCAACAAC
AAGGACAATGTCGAAAAGCAAAGAGAGCGCCTTAAGCTAGTTCTTTCGGCTAATCCGATTTGTTTCAACCATAATCTTGA
AACTGTCAAAAGACTTCAAGGAGAAGTCAGGAGAGGTGCTACTTACCAACACTCACTCAATCTTCTCAAATTTGCTAGAG
AAATCGATCCAAAAATTCCTACAAAATCAGGAATAATGCTTGGACTGGGAGAAAAATTTCATGAAATAATTCAAACCCTT
AAAGACCTTAGGAAAGTAGACTGTCAATATTTGACTATCGGTCAATATTTACGACCTTCACTTGCTCACATTCCAGTTTC
TCATTATTGGTCACCAACAAAATTCAAGGATTTTGCAGAAATAGCAAAGGGATTAGGTTTTAAGAAAGTAAACAGCGGTC
CTTTAGTAAGAAGCAGCTACCATGCGAATGAGACCTTAGATTAG

Upstream 100 bases:

>100_bases
CTTATGGACTTCCTGTTGGAAGTGAGTTGGAATATGCCGATGAAGTAACACTCTCACGTGCTTTAGAAGGTCGTAGAGAC
TTAGATTAACTATCAAACAC

Downstream 100 bases:

>100_bases
CCTCCTTAAAAAATCTAACTACTCCGACCAACTCCGATAACTTCTTTCAAACACTTTCTCACAATTACCTACCATAAAAG
CACCTGCGCCTCCCCAAACA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 2; Lipoate synthase 2; Lipoic acid synthase 2; Sulfur insertion protein lipA2 [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTTTTRKKTKYSSFLPEERLPNWIKPSIGNATQLEKVQKLVKENRLHTICEEGRCPNRGECYAAGTATFLLGGSICTRSC
AFCQVEKGKSPEKVNIFEAEKVANAVQVLNLKYVVLTAVARDDLPDHGASLFTTTMDAIRSLNPGVAIEVLTPDFWGGNN
KDNVEKQRERLKLVLSANPICFNHNLETVKRLQGEVRRGATYQHSLNLLKFAREIDPKIPTKSGIMLGLGEKFHEIIQTL
KDLRKVDCQYLTIGQYLRPSLAHIPVSHYWSPTKFKDFAEIAKGLGFKKVNSGPLVRSSYHANETLD

Sequences:

>Translated_307_residues
MTTTTRKKTKYSSFLPEERLPNWIKPSIGNATQLEKVQKLVKENRLHTICEEGRCPNRGECYAAGTATFLLGGSICTRSC
AFCQVEKGKSPEKVNIFEAEKVANAVQVLNLKYVVLTAVARDDLPDHGASLFTTTMDAIRSLNPGVAIEVLTPDFWGGNN
KDNVEKQRERLKLVLSANPICFNHNLETVKRLQGEVRRGATYQHSLNLLKFAREIDPKIPTKSGIMLGLGEKFHEIIQTL
KDLRKVDCQYLTIGQYLRPSLAHIPVSHYWSPTKFKDFAEIAKGLGFKKVNSGPLVRSSYHANETLD
>Mature_306_residues
TTTTRKKTKYSSFLPEERLPNWIKPSIGNATQLEKVQKLVKENRLHTICEEGRCPNRGECYAAGTATFLLGGSICTRSCA
FCQVEKGKSPEKVNIFEAEKVANAVQVLNLKYVVLTAVARDDLPDHGASLFTTTMDAIRSLNPGVAIEVLTPDFWGGNNK
DNVEKQRERLKLVLSANPICFNHNLETVKRLQGEVRRGATYQHSLNLLKFAREIDPKIPTKSGIMLGLGEKFHEIIQTLK
DLRKVDCQYLTIGQYLRPSLAHIPVSHYWSPTKFKDFAEIAKGLGFKKVNSGPLVRSSYHANETLD

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=292, Percent_Identity=41.7808219178082, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI37577164, Length=255, Percent_Identity=40.3921568627451, Blast_Score=192, Evalue=4e-49,
Organism=Escherichia coli, GI1786846, Length=286, Percent_Identity=43.7062937062937, Blast_Score=249, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI32564533, Length=295, Percent_Identity=42.0338983050847, Blast_Score=218, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=295, Percent_Identity=42.3728813559322, Blast_Score=224, Evalue=9e-60,
Organism=Drosophila melanogaster, GI221513272, Length=292, Percent_Identity=41.4383561643836, Blast_Score=224, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34417; Mature: 34286

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTTRKKTKYSSFLPEERLPNWIKPSIGNATQLEKVQKLVKENRLHTICEEGRCPNRGE
CCCCCCCHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
CYAAGTATFLLGGSICTRSCAFCQVEKGKSPEKVNIFEAEKVANAVQVLNLKYVVLTAVA
EEECCCHHHHCCCHHHHCCHHHEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
RDDLPDHGASLFTTTMDAIRSLNPGVAIEVLTPDFWGGNNKDNVEKQRERLKLVLSANPI
HCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHEEHEECCCE
CFNHNLETVKRLQGEVRRGATYQHSLNLLKFAREIDPKIPTKSGIMLGLGEKFHEIIQTL
EECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHH
KDLRKVDCQYLTIGQYLRPSLAHIPVSHYWSPTKFKDFAEIAKGLGFKKVNSGPLVRSSY
HHHHHCCCCEEEHHHHHCCHHHHCCHHHCCCCCHHHHHHHHHHCCCCEECCCCCCEECCC
HANETLD
CCCCCCC
>Mature Secondary Structure 
TTTTRKKTKYSSFLPEERLPNWIKPSIGNATQLEKVQKLVKENRLHTICEEGRCPNRGE
CCCCCCHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
CYAAGTATFLLGGSICTRSCAFCQVEKGKSPEKVNIFEAEKVANAVQVLNLKYVVLTAVA
EEECCCHHHHCCCHHHHCCHHHEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
RDDLPDHGASLFTTTMDAIRSLNPGVAIEVLTPDFWGGNNKDNVEKQRERLKLVLSANPI
HCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHEEHEECCCE
CFNHNLETVKRLQGEVRRGATYQHSLNLLKFAREIDPKIPTKSGIMLGLGEKFHEIIQTL
EECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHH
KDLRKVDCQYLTIGQYLRPSLAHIPVSHYWSPTKFKDFAEIAKGLGFKKVNSGPLVRSSY
HHHHHCCCCEEEHHHHHCCHHHHCCHHHCCCCCHHHHHHHHHHCCCCEECCCCCCEECCC
HANETLD
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]