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The map label for this gene is sphR [H]

Identifier: 124026175

GI number: 124026175

Start: 1176805

End: 1177569

Strand: Reverse

Name: sphR [H]

Synonym: NATL1_14691

Alternate gene names: 124026175

Gene position: 1177569-1176805 (Counterclockwise)

Preceding gene: 124026177

Following gene: 124026172

Centisome position: 63.15

GC content: 46.67

Gene sequence:

>765_bases
GTGACATTAATACCTGCAGATCAGCTTGCTACTGAGCAAATCCAAGGATCTCCCTCAGGTTCTTCTCCTATTCAAGCAAC
CACACAATCACCATCCAAGGTGCTTGTTGTAGAACCACATCCAACTCTCAGAACTGTTTTGGTTCAGAGGCTCAGGCAAG
ATGGACAGCTGGCCGCAGCAGTAGGTTCAGCTGAGGAGGCCGTTGACCTATGTCGAGACCAATCACCTGATTTATTAGTT
AGTGCAGAGATATTAGAAAAAAGCTCTGCTCTCCGACTGGCACAACAATTGGGATGTTCAGTGATTGTTCTAACAGCAAG
AACAGGAGTTGAACCTGTTGTAGGCCTTTTAGATGATGGTGCTGATGATGTTTTAAGGAAACCTTTTGGCTTGGAAGAAC
TAGCTGCAAGATGCAGGACTTTACTTAAGAGAGGAAGAATTGGTTTACAAGAACGTGTCGCTGTTGGTCCTCTTGAGGTT
CATCTACTTCTCAGACAGGTCACCTTGCGAGAGAAACCTGTAGAACTAAGTCCAAGGGAATTTGCTTTGTTGTGTGCCTT
GTTGATGCCGCCTGGCATGGTAAGAAGCCGTCATGAGCTGCTACGAATGGCTTGGCCGCCGTTTAGCGGTGGTCCTCGCT
CAGTTGATACGCAGGTTTTGACGCTTAGAAGAAAACTGGAACAAGCAGGTCTAGGTGATGGCGGAGGAATTACAACTGTT
CGCCAGCAGGGCTATAGGTTTAGTCTTGATAATTTGCCGTCATAA

Upstream 100 bases:

>100_bases
AAAAGATGTCATGGTTTGCTAAGACAGAGACTATTCTTGATAGTCAGCGAACAGTTTGCTTTAATATCTTTTCCAGTCTA
AGGGTCTACTTTTCTAAGTT

Downstream 100 bases:

>100_bases
TTTCTAGAATTATTTTTTAATTATTCTGGTAGCTCCCATTTGTTCATACCAAGCTTTGTGCCTATCTTGTGAGCACAGGC
ATAACCACTGAAAGCCACGG

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTLIPADQLATEQIQGSPSGSSPIQATTQSPSKVLVVEPHPTLRTVLVQRLRQDGQLAAAVGSAEEAVDLCRDQSPDLLV
SAEILEKSSALRLAQQLGCSVIVLTARTGVEPVVGLLDDGADDVLRKPFGLEELAARCRTLLKRGRIGLQERVAVGPLEV
HLLLRQVTLREKPVELSPREFALLCALLMPPGMVRSRHELLRMAWPPFSGGPRSVDTQVLTLRRKLEQAGLGDGGGITTV
RQQGYRFSLDNLPS

Sequences:

>Translated_254_residues
MTLIPADQLATEQIQGSPSGSSPIQATTQSPSKVLVVEPHPTLRTVLVQRLRQDGQLAAAVGSAEEAVDLCRDQSPDLLV
SAEILEKSSALRLAQQLGCSVIVLTARTGVEPVVGLLDDGADDVLRKPFGLEELAARCRTLLKRGRIGLQERVAVGPLEV
HLLLRQVTLREKPVELSPREFALLCALLMPPGMVRSRHELLRMAWPPFSGGPRSVDTQVLTLRRKLEQAGLGDGGGITTV
RQQGYRFSLDNLPS
>Mature_253_residues
TLIPADQLATEQIQGSPSGSSPIQATTQSPSKVLVVEPHPTLRTVLVQRLRQDGQLAAAVGSAEEAVDLCRDQSPDLLVS
AEILEKSSALRLAQQLGCSVIVLTARTGVEPVVGLLDDGADDVLRKPFGLEELAARCRTLLKRGRIGLQERVAVGPLEVH
LLLRQVTLREKPVELSPREFALLCALLMPPGMVRSRHELLRMAWPPFSGGPRSVDTQVLTLRRKLEQAGLGDGGGITTVR
QQGYRFSLDNLPS

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=31.1111111111111, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788394, Length=236, Percent_Identity=29.6610169491525, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=33.9366515837104, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=28.2511210762332, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789809, Length=236, Percent_Identity=32.6271186440678, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786911, Length=230, Percent_Identity=31.7391304347826, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790552, Length=147, Percent_Identity=36.0544217687075, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=30, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787229, Length=214, Percent_Identity=29.9065420560748, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=27.3542600896861, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 27468; Mature: 27337

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIPADQLATEQIQGSPSGSSPIQATTQSPSKVLVVEPHPTLRTVLVQRLRQDGQLAAA
CCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCEEHH
VGSAEEAVDLCRDQSPDLLVSAEILEKSSALRLAQQLGCSVIVLTARTGVEPVVGLLDDG
HCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCC
ADDVLRKPFGLEELAARCRTLLKRGRIGLQERVAVGPLEVHLLLRQVTLREKPVELSPRE
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHH
FALLCALLMPPGMVRSRHELLRMAWPPFSGGPRSVDTQVLTLRRKLEQAGLGDGGGITTV
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
RQQGYRFSLDNLPS
HHCCCEECCCCCCC
>Mature Secondary Structure 
TLIPADQLATEQIQGSPSGSSPIQATTQSPSKVLVVEPHPTLRTVLVQRLRQDGQLAAA
CCCCCHHHHHHHHCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCEEHH
VGSAEEAVDLCRDQSPDLLVSAEILEKSSALRLAQQLGCSVIVLTARTGVEPVVGLLDDG
HCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCC
ADDVLRKPFGLEELAARCRTLLKRGRIGLQERVAVGPLEVHLLLRQVTLREKPVELSPRE
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHH
FALLCALLMPPGMVRSRHELLRMAWPPFSGGPRSVDTQVLTLRRKLEQAGLGDGGGITTV
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
RQQGYRFSLDNLPS
HHCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]