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The map label for this gene is phoB [H]

Identifier: 124025854

GI number: 124025854

Start: 1023632

End: 1024369

Strand: Direct

Name: phoB [H]

Synonym: NATL1_11471

Alternate gene names: 124025854

Gene position: 1023632-1024369 (Clockwise)

Preceding gene: 124025853

Following gene: 124025855

Centisome position: 54.89

GC content: 38.35

Gene sequence:

>738_bases
GTGACCAGTCCTGTCTCTGACAAGATATCCAAGGCTAAAAGACTACTTATCGTTGAAGACGATAAGAGCATTCGCGAAAC
GCTCCGTGAAGCTCTTGTTGCAGAGGGTTATCAAGTCGAGGTTTGCAAGACTGGTGATGAAGCTGAGGCTAGAGTTTTCT
CTCAGTTCGACACACGTAAAAAGATTGATTTAATTGTTTTAGACTTAATGTTACCCAACCTGAATGGAATAGAGCTTTGT
AGAAGAGTCCGAAAAAACAATATAAAAACACCTATTCTTATTGTCAGCGCAAAAGACAGTGAATCTGATCGTGTATTGGG
ATTAGAAGTTGGAGCTGACGATTATTTGATAAAGCCTTTTGGCTTAAATGAACTAATTGCAAGGTGTCGAGCAATTCTCA
GAAGATCTGAAACTAATCATGAGAAAACTCAAGATTTATCCCAAGTCCTTAGCTACAAAAATATATCCTTATACAGACAG
GAATACAGGGTCACAAAAAACAGTAAGGAAATAAATCTTGCCCCTAAGGAATACAAAATCATGGAATTGTTCCTGCAGAA
TCCAAAGCGGGTGTGGAGTCGTGATCAACTTTTAGAACAAATTTGGGGGATTGATTATGTGGGGGATTCTAAAACTGTAG
ATGTACATATTAGGTGGATTAGAGAAAAAATAGAGGATGATGCATCGGATCCAGACTATATAAGAACCGTTAGAGGTTTT
GGCTACAAATTTGGGTAA

Upstream 100 bases:

>100_bases
CTCAACTATATAAGTTTTTTTTTAATGATCAGCTCACTTCATTCAATTTTCTTATAAATCAACTATAATTGATATAATCA
ACAATGTTAGCTTAACCTCT

Downstream 100 bases:

>100_bases
AGACGAATTATGCTAAAAAAAATATTAAAAAAGCTACTCAAATTCATAAAAAAGATTCTCAGCATTAGAGGAGTTGGAAC
GACTATAAGAAATGTTAGAA

Product: two-component response regulator, phosphate

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTSPVSDKISKAKRLLIVEDDKSIRETLREALVAEGYQVEVCKTGDEAEARVFSQFDTRKKIDLIVLDLMLPNLNGIELC
RRVRKNNIKTPILIVSAKDSESDRVLGLEVGADDYLIKPFGLNELIARCRAILRRSETNHEKTQDLSQVLSYKNISLYRQ
EYRVTKNSKEINLAPKEYKIMELFLQNPKRVWSRDQLLEQIWGIDYVGDSKTVDVHIRWIREKIEDDASDPDYIRTVRGF
GYKFG

Sequences:

>Translated_245_residues
MTSPVSDKISKAKRLLIVEDDKSIRETLREALVAEGYQVEVCKTGDEAEARVFSQFDTRKKIDLIVLDLMLPNLNGIELC
RRVRKNNIKTPILIVSAKDSESDRVLGLEVGADDYLIKPFGLNELIARCRAILRRSETNHEKTQDLSQVLSYKNISLYRQ
EYRVTKNSKEINLAPKEYKIMELFLQNPKRVWSRDQLLEQIWGIDYVGDSKTVDVHIRWIREKIEDDASDPDYIRTVRGF
GYKFG
>Mature_244_residues
TSPVSDKISKAKRLLIVEDDKSIRETLREALVAEGYQVEVCKTGDEAEARVFSQFDTRKKIDLIVLDLMLPNLNGIELCR
RVRKNNIKTPILIVSAKDSESDRVLGLEVGADDYLIKPFGLNELIARCRAILRRSETNHEKTQDLSQVLSYKNISLYRQE
YRVTKNSKEINLAPKEYKIMELFLQNPKRVWSRDQLLEQIWGIDYVGDSKTVDVHIRWIREKIEDDASDPDYIRTVRGFG
YKFG

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=235, Percent_Identity=35.7446808510638, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=35.6223175965665, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1786911, Length=230, Percent_Identity=36.0869565217391, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1786784, Length=229, Percent_Identity=35.3711790393013, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1788394, Length=233, Percent_Identity=34.7639484978541, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1789402, Length=232, Percent_Identity=34.051724137931, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1790860, Length=229, Percent_Identity=34.9344978165939, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1790552, Length=231, Percent_Identity=35.9307359307359, Blast_Score=133, Evalue=9e-33,
Organism=Escherichia coli, GI1790863, Length=238, Percent_Identity=33.1932773109244, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI87082012, Length=231, Percent_Identity=32.034632034632, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787375, Length=234, Percent_Identity=35.4700854700855, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI2367329, Length=231, Percent_Identity=32.4675324675325, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI145693140, Length=237, Percent_Identity=29.535864978903, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787229, Length=234, Percent_Identity=32.0512820512821, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1788550, Length=117, Percent_Identity=29.0598290598291, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790299, Length=128, Percent_Identity=27.34375, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28411; Mature: 28280

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPVSDKISKAKRLLIVEDDKSIRETLREALVAEGYQVEVCKTGDEAEARVFSQFDTRK
CCCCHHHHHHHHHEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCH
KIDLIVLDLMLPNLNGIELCRRVRKNNIKTPILIVSAKDSESDRVLGLEVGADDYLIKPF
HHHHEEEEHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCEEECCC
GLNELIARCRAILRRSETNHEKTQDLSQVLSYKNISLYRQEYRVTKNSKEINLAPKEYKI
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHH
MELFLQNPKRVWSRDQLLEQIWGIDYVGDSKTVDVHIRWIREKIEDDASDPDYIRTVRGF
HHHHHHCHHHHHHHHHHHHHHCCCEECCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHC
GYKFG
CCCCC
>Mature Secondary Structure 
TSPVSDKISKAKRLLIVEDDKSIRETLREALVAEGYQVEVCKTGDEAEARVFSQFDTRK
CCCHHHHHHHHHEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCH
KIDLIVLDLMLPNLNGIELCRRVRKNNIKTPILIVSAKDSESDRVLGLEVGADDYLIKPF
HHHHEEEEHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCEEECCC
GLNELIARCRAILRRSETNHEKTQDLSQVLSYKNISLYRQEYRVTKNSKEINLAPKEYKI
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHH
MELFLQNPKRVWSRDQLLEQIWGIDYVGDSKTVDVHIRWIREKIEDDASDPDYIRTVRGF
HHHHHHCHHHHHHHHHHHHHHCCCEECCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHC
GYKFG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]