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The map label for this gene is tpiA

Identifier: 124025771

GI number: 124025771

Start: 983402

End: 984142

Strand: Reverse

Name: tpiA

Synonym: NATL1_10641

Alternate gene names: 124025771

Gene position: 984142-983402 (Counterclockwise)

Preceding gene: 124025772

Following gene: 124025770

Centisome position: 52.78

GC content: 38.19

Gene sequence:

>741_bases
GTGCGTAAACCGGTCATTGCAGGGAATTGGAAGATGAACATGACGTGTACCGAAGCGATTGAGTACATGCGTGTATTAAT
TCCACTACTGAAAGATATTCCGAAAAAAGATAGAGAAGTTGTTATTGCACCCCCATTTACAGCCCTATACCCTCTGTCTG
AGTTTATTAGGGACAAAAATGATTGTCTATCGTTATCTAGCCAAAATGTTCATTGGGAGGATAGTGGGGCCTATACAGCA
GAAGTTTCTCCCCTAATGCTTAATGAGCTTTCGGTTAAGTGTGCAATCGTTGGACACAGTGAACCTCGTAAATATTTTAG
TGAAAGTGATGAACAAATTAATAAAAGAGCAAAATCGGCGCAAGATCATCAGTTAATTCCAATTGTTTGTGTTGGAGAGA
CTTTTCAACAAAGAGAAATGGGGGAAGCAGAAAGGGTTATACGAAGGCAAATTGAACAAGGTCTTGAAGGAATTGAAGTA
AAAAAACTAATAGTTGCTTATGAACCAATCTGGGCTATCGGTACTGGTAAAACTTGTGAAGCTAATGAGGCTAATCGAAT
TTGTGGTCTTATTCGTAAGTGGATTGGTTACGAAGACGTGATTATTCAATATGGTGGATCAGTAAAATCAAATAATATTG
ATGAAATCATGTCCATGTCAGATATTGATGGTGTACTAGTTGGTGGAGCCTCTCTAGATCCTACAAATTTTGCCCGAATT
GCAAACTACGAAAAAATCTAA

Upstream 100 bases:

>100_bases
TGATGGAGATCAAATTTCTTTTGCAAATGAAACATACATTGTTCCAAATTCTGATCCTCTAGGCCGTAAGTTGGCAAGAA
GCGAAAAATGAGGAGCTAGC

Downstream 100 bases:

>100_bases
TAATTTCCCGCCTAAGCATTGGGAACGAGAAACCCAATTAATGGCAATAGTAAACATCACTGAAGATTCTTTTAGCGATG
GAGGACTGTACATAGACTTA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MRKPVIAGNWKMNMTCTEAIEYMRVLIPLLKDIPKKDREVVIAPPFTALYPLSEFIRDKNDCLSLSSQNVHWEDSGAYTA
EVSPLMLNELSVKCAIVGHSEPRKYFSESDEQINKRAKSAQDHQLIPIVCVGETFQQREMGEAERVIRRQIEQGLEGIEV
KKLIVAYEPIWAIGTGKTCEANEANRICGLIRKWIGYEDVIIQYGGSVKSNNIDEIMSMSDIDGVLVGGASLDPTNFARI
ANYEKI

Sequences:

>Translated_246_residues
MRKPVIAGNWKMNMTCTEAIEYMRVLIPLLKDIPKKDREVVIAPPFTALYPLSEFIRDKNDCLSLSSQNVHWEDSGAYTA
EVSPLMLNELSVKCAIVGHSEPRKYFSESDEQINKRAKSAQDHQLIPIVCVGETFQQREMGEAERVIRRQIEQGLEGIEV
KKLIVAYEPIWAIGTGKTCEANEANRICGLIRKWIGYEDVIIQYGGSVKSNNIDEIMSMSDIDGVLVGGASLDPTNFARI
ANYEKI
>Mature_246_residues
MRKPVIAGNWKMNMTCTEAIEYMRVLIPLLKDIPKKDREVVIAPPFTALYPLSEFIRDKNDCLSLSSQNVHWEDSGAYTA
EVSPLMLNELSVKCAIVGHSEPRKYFSESDEQINKRAKSAQDHQLIPIVCVGETFQQREMGEAERVIRRQIEQGLEGIEV
KKLIVAYEPIWAIGTGKTCEANEANRICGLIRKWIGYEDVIIQYGGSVKSNNIDEIMSMSDIDGVLVGGASLDPTNFARI
ANYEKI

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=253, Percent_Identity=36.7588932806324, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI4507645, Length=253, Percent_Identity=36.7588932806324, Blast_Score=146, Evalue=2e-35,
Organism=Escherichia coli, GI1790353, Length=251, Percent_Identity=39.4422310756972, Blast_Score=160, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17536593, Length=250, Percent_Identity=34.8, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320255, Length=255, Percent_Identity=36.078431372549, Blast_Score=133, Evalue=3e-32,
Organism=Drosophila melanogaster, GI28572008, Length=250, Percent_Identity=39.2, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI28572006, Length=250, Percent_Identity=39.2, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI28572004, Length=242, Percent_Identity=39.2561983471074, Blast_Score=157, Evalue=6e-39,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_PROM1 (A2C2B2)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014887.1
- ProteinModelPortal:   A2C2B2
- SMR:   A2C2B2
- STRING:   A2C2B2
- GeneID:   4779120
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_10641
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   PMAR167555:NATL1_10641-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27668; Mature: 27668

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 99-99 ACT_SITE 168-168 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPVIAGNWKMNMTCTEAIEYMRVLIPLLKDIPKKDREVVIAPPFTALYPLSEFIRDKN
CCCCEEECCEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCH
DCLSLSSQNVHWEDSGAYTAEVSPLMLNELSVKCAIVGHSEPRKYFSESDEQINKRAKSA
HHCCCCCCCCEECCCCCEEEECCHHHHHCCEEEEEEEECCCHHHHHCCCHHHHHHHHHCC
QDHQLIPIVCVGETFQQREMGEAERVIRRQIEQGLEGIEVKKLIVAYEPIWAIGTGKTCE
CCCCEEEEEECCCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHEEEECCEEEECCCCCCC
ANEANRICGLIRKWIGYEDVIIQYGGSVKSNNIDEIMSMSDIDGVLVGGASLDPTNFARI
CCCHHHHHHHHHHHCCHHHEEEEECCCCCCCCHHHHHHHHCCCEEEECCCCCCCCHHHHH
ANYEKI
CCCCCC
>Mature Secondary Structure
MRKPVIAGNWKMNMTCTEAIEYMRVLIPLLKDIPKKDREVVIAPPFTALYPLSEFIRDKN
CCCCEEECCEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCH
DCLSLSSQNVHWEDSGAYTAEVSPLMLNELSVKCAIVGHSEPRKYFSESDEQINKRAKSA
HHCCCCCCCCEECCCCCEEEECCHHHHHCCEEEEEEEECCCHHHHHCCCHHHHHHHHHCC
QDHQLIPIVCVGETFQQREMGEAERVIRRQIEQGLEGIEVKKLIVAYEPIWAIGTGKTCE
CCCCEEEEEECCCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHEEEECCEEEECCCCCCC
ANEANRICGLIRKWIGYEDVIIQYGGSVKSNNIDEIMSMSDIDGVLVGGASLDPTNFARI
CCCHHHHHHHHHHHCCHHHEEEEECCCCCCCCHHHHHHHHCCCEEEECCCCCCCCHHHHH
ANYEKI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA