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The map label for this gene is hisF

Identifier: 124025196

GI number: 124025196

Start: 439725

End: 440495

Strand: Direct

Name: hisF

Synonym: NATL1_04831

Alternate gene names: 124025196

Gene position: 439725-440495 (Clockwise)

Preceding gene: 124025193

Following gene: 124025197

Centisome position: 23.58

GC content: 40.6

Gene sequence:

>771_bases
ATGGTTGCTTTAAGATTGATTCCTTGTCTTGATGTTTCAAACGGACGAGTGGTTAAGGGGGTTAATTTTGTTGGATTGCG
TGATGCAGGTGATCCAGTTGAACTTGGATGTAGATATAGCAAGGCTGGAGCTGATGAATTGGTCTTCCTTGACATAACAG
CTACGCACGAGAAAAGATCGACTTTGGTTGATATGGTTAGACGAACATCCGAAGCGGTAACCATTCCTTTTACTGTTGGA
GGGGGGATCAGCTCATTGAATGGAATTAATGAATTGCTACGAGCAGGAGCTGACAAGGTAAGTTTGAATTCCAGTGCTGT
TAAAGACCCTTCATTGATTTCTCAAGGGGCAAATCGTTTTGGATCGCAATGTATTGTGGTCGCAATAGATGCGAAAAAGA
ACAAACATATTCCCAATAAGTGGGACGTTTATGTGAGTGGAGGACGGAAAAATACAGGCTTGGATGCGATTGAATGGGCA
GAAAAAATATTTGAGATGGGAGCAGGTGAAATCCTTTTAACCTCCATGGACGGTGATGGAACTCAAAATGGTTATGACAT
AGAACTGACCAGATGTATTTCTGAGAAAGTTTCAATACCAGTAATAGCCTCAGGAGGAGCTGGTTCTTTGAGTCACATTA
AAGATGCTTTTACTCTTGGGAAATCTTCTGCGGCTCTCTTGGCTTCCTTATTGCATGATGGGCAATTAACTATCAGAGAA
ATAAAAGAATATTTGATTAAGGAAAATTTACCCATAAGACCAATTGAATGA

Upstream 100 bases:

>100_bases
AATCTTAATTTTTGAACCTATCCTTGAAAGGGAGGTTGGCTTAGCCATGGAATTAAATTACTTGATTTCGGCACCTTAAC
AGTTCCTCTGTGTAAAAATC

Downstream 100 bases:

>100_bases
TAAATATTCTCATGAAAAGATGTAAAATCGATTTTTTTCACCTTAAAATTTTTTTGCTATCTAAATTTTTTTCTGTATTT
ACATGAGGCCTGGTAATACA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MVALRLIPCLDVSNGRVVKGVNFVGLRDAGDPVELGCRYSKAGADELVFLDITATHEKRSTLVDMVRRTSEAVTIPFTVG
GGISSLNGINELLRAGADKVSLNSSAVKDPSLISQGANRFGSQCIVVAIDAKKNKHIPNKWDVYVSGGRKNTGLDAIEWA
EKIFEMGAGEILLTSMDGDGTQNGYDIELTRCISEKVSIPVIASGGAGSLSHIKDAFTLGKSSAALLASLLHDGQLTIRE
IKEYLIKENLPIRPIE

Sequences:

>Translated_256_residues
MVALRLIPCLDVSNGRVVKGVNFVGLRDAGDPVELGCRYSKAGADELVFLDITATHEKRSTLVDMVRRTSEAVTIPFTVG
GGISSLNGINELLRAGADKVSLNSSAVKDPSLISQGANRFGSQCIVVAIDAKKNKHIPNKWDVYVSGGRKNTGLDAIEWA
EKIFEMGAGEILLTSMDGDGTQNGYDIELTRCISEKVSIPVIASGGAGSLSHIKDAFTLGKSSAALLASLLHDGQLTIRE
IKEYLIKENLPIRPIE
>Mature_256_residues
MVALRLIPCLDVSNGRVVKGVNFVGLRDAGDPVELGCRYSKAGADELVFLDITATHEKRSTLVDMVRRTSEAVTIPFTVG
GGISSLNGINELLRAGADKVSLNSSAVKDPSLISQGANRFGSQCIVVAIDAKKNKHIPNKWDVYVSGGRKNTGLDAIEWA
EKIFEMGAGEILLTSMDGDGTQNGYDIELTRCISEKVSIPVIASGGAGSLSHIKDAFTLGKSSAALLASLLHDGQLTIRE
IKEYLIKENLPIRPIE

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=44.7470817120623, Blast_Score=210, Evalue=7e-56,
Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=27.9166666666667, Blast_Score=91, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=310, Percent_Identity=32.5806451612903, Blast_Score=151, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_PROM1 (A2C0N7)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014312.1
- ProteinModelPortal:   A2C0N7
- SMR:   A2C0N7
- STRING:   A2C0N7
- GeneID:   4780366
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_04831
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   PMAR167555:NATL1_04831-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27336; Mature: 27336

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVALRLIPCLDVSNGRVVKGVNFVGLRDAGDPVELGCRYSKAGADELVFLDITATHEKRS
CCEEEEEEEEECCCCCEEECEEEEEECCCCCCHHHCEEECCCCCCCEEEEEEECCCHHHH
TLVDMVRRTSEAVTIPFTVGGGISSLNGINELLRAGADKVSLNSSAVKDPSLISQGANRF
HHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHC
GSQCIVVAIDAKKNKHIPNKWDVYVSGGRKNTGLDAIEWAEKIFEMGAGEILLTSMDGDG
CCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TQNGYDIELTRCISEKVSIPVIASGGAGSLSHIKDAFTLGKSSAALLASLLHDGQLTIRE
CCCCEEEEHEEEHHHHCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHH
IKEYLIKENLPIRPIE
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVALRLIPCLDVSNGRVVKGVNFVGLRDAGDPVELGCRYSKAGADELVFLDITATHEKRS
CCEEEEEEEEECCCCCEEECEEEEEECCCCCCHHHCEEECCCCCCCEEEEEEECCCHHHH
TLVDMVRRTSEAVTIPFTVGGGISSLNGINELLRAGADKVSLNSSAVKDPSLISQGANRF
HHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHC
GSQCIVVAIDAKKNKHIPNKWDVYVSGGRKNTGLDAIEWAEKIFEMGAGEILLTSMDGDG
CCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TQNGYDIELTRCISEKVSIPVIASGGAGSLSHIKDAFTLGKSSAALLASLLHDGQLTIRE
CCCCEEEEHEEEHHHHCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHH
IKEYLIKENLPIRPIE
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA