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The map label for this gene is capI [H]

Identifier: 124025108

GI number: 124025108

Start: 365177

End: 366223

Strand: Direct

Name: capI [H]

Synonym: NATL1_03951

Alternate gene names: 124025108

Gene position: 365177-366223 (Clockwise)

Preceding gene: 124025107

Following gene: 124025119

Centisome position: 19.58

GC content: 34.48

Gene sequence:

>1047_bases
ATGAATTCAAAGGCAAGTAATGAGACAATTCTTGTAACAGGAGCCGCAGGTTTTATTGGCGCGGCTTTAGTAAAAGCTCT
TCTTAACTTGGATTTTAAAGTTATAGGTATTGATAACTTAAATGACTATTATTCTACTTCCTTAAAGAGATCCAGATTAA
CTGAAATTGAAAAAGTTTCCACGGTAAATGGAGAATGGTTTTTTTATGAGATTCCTATAGAAGATAATAAAGTATTGCAA
GACATAATTAATAGATACAATCCACAAGTTTTTGTTCATCTTGCCGCACAAGCGGGTGTTCGTTATTCAATAACGAACCC
TGCTGCATATATACAAAGTAATTTGGTGGGTTTTGCAAATGTATTGGAAGGATGTAGGCAGAATCAAATCCCTCATTTGA
TTTATGCATCTAGCAGTTCTGTTTATGGTGGCAATAAAAATCTTCCTTTCTATGAAGAACAAGCCGTAAATCATCCAGTT
AGTTTGTATGCAGCTACTAAGAAATCTAATGAATTAATGGCCCATACCTATAGCCACTTATATGATTTACCAACAACTGG
ACTACGATTCTTTACCGTTTATGGCCCATGGGGAAGACCCGATATGGCTCCAATGATTTTTGCGAGATCAATTTTAAATA
ATGAGCCAATCCAAGTATTTAATTACGGTAAAATGCAAAGGGATTTTACTTACATCGATGATGTAGTAGAGGGGATAATT
CGTTGTTGCTTCAAGAAAGCAAGTATTGATGATGAATTTAATCCTCTTGTTCCTAACCCTTCAACTTCATCAGCACCTTA
TAGGATTTTTAATATAGGTAATTCACGTCCAACTCAACTCACATATTTTATAGAGTTATTAGAGAAGAATTTGGGAAAGA
AAGCTATAAAGAATTTTCAACCTATGCAGCCGGGAGATGTAGTTTCTACTGCTGCAAGGATGGACTTGCTTAATTCGTGG
GTAGATTACAAACCCATAACATCCATAGAAAATGGTATTAAATTGTTCTCTGAATGGTACTTAGACTATTTTAAAAATAC
TTTTTGA

Upstream 100 bases:

>100_bases
AAAAGTAAATAAGTAATTAACTATTTTAAATTAAAAGTTAATGAATAAGATGCTATGATGATATTCAAATTTAATATTTT
TTGTAAATTATAATAAACCC

Downstream 100 bases:

>100_bases
ACACTGGGTTTGATTTTTCTGTTTATTTAAAACTTAGGCTAGTCCTAATAATTTAATTCTGAAGAGAGCAACATTTTTTG
ATTGGATTGGATTTGAAATT

Product: putative nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MNSKASNETILVTGAAGFIGAALVKALLNLDFKVIGIDNLNDYYSTSLKRSRLTEIEKVSTVNGEWFFYEIPIEDNKVLQ
DIINRYNPQVFVHLAAQAGVRYSITNPAAYIQSNLVGFANVLEGCRQNQIPHLIYASSSSVYGGNKNLPFYEEQAVNHPV
SLYAATKKSNELMAHTYSHLYDLPTTGLRFFTVYGPWGRPDMAPMIFARSILNNEPIQVFNYGKMQRDFTYIDDVVEGII
RCCFKKASIDDEFNPLVPNPSTSSAPYRIFNIGNSRPTQLTYFIELLEKNLGKKAIKNFQPMQPGDVVSTAARMDLLNSW
VDYKPITSIENGIKLFSEWYLDYFKNTF

Sequences:

>Translated_348_residues
MNSKASNETILVTGAAGFIGAALVKALLNLDFKVIGIDNLNDYYSTSLKRSRLTEIEKVSTVNGEWFFYEIPIEDNKVLQ
DIINRYNPQVFVHLAAQAGVRYSITNPAAYIQSNLVGFANVLEGCRQNQIPHLIYASSSSVYGGNKNLPFYEEQAVNHPV
SLYAATKKSNELMAHTYSHLYDLPTTGLRFFTVYGPWGRPDMAPMIFARSILNNEPIQVFNYGKMQRDFTYIDDVVEGII
RCCFKKASIDDEFNPLVPNPSTSSAPYRIFNIGNSRPTQLTYFIELLEKNLGKKAIKNFQPMQPGDVVSTAARMDLLNSW
VDYKPITSIENGIKLFSEWYLDYFKNTF
>Mature_348_residues
MNSKASNETILVTGAAGFIGAALVKALLNLDFKVIGIDNLNDYYSTSLKRSRLTEIEKVSTVNGEWFFYEIPIEDNKVLQ
DIINRYNPQVFVHLAAQAGVRYSITNPAAYIQSNLVGFANVLEGCRQNQIPHLIYASSSSVYGGNKNLPFYEEQAVNHPV
SLYAATKKSNELMAHTYSHLYDLPTTGLRFFTVYGPWGRPDMAPMIFARSILNNEPIQVFNYGKMQRDFTYIDDVVEGII
RCCFKKASIDDEFNPLVPNPSTSSAPYRIFNIGNSRPTQLTYFIELLEKNLGKKAIKNFQPMQPGDVVSTAARMDLLNSW
VDYKPITSIENGIKLFSEWYLDYFKNTF

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=346, Percent_Identity=23.4104046242775, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI56237023, Length=352, Percent_Identity=24.1477272727273, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI56118217, Length=352, Percent_Identity=24.1477272727273, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI189083684, Length=352, Percent_Identity=24.1477272727273, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=23.6686390532544, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788353, Length=360, Percent_Identity=24.4444444444444, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=23.5632183908046, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1786974, Length=332, Percent_Identity=23.4939759036145, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=353, Percent_Identity=23.7960339943343, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=353, Percent_Identity=23.7960339943343, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=245, Percent_Identity=26.9387755102041, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=248, Percent_Identity=26.6129032258064, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=365, Percent_Identity=21.6438356164384, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19923002, Length=349, Percent_Identity=25.7879656160458, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356223, Length=350, Percent_Identity=26.8571428571429, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 39382; Mature: 39382

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKASNETILVTGAAGFIGAALVKALLNLDFKVIGIDNLNDYYSTSLKRSRLTEIEKVS
CCCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
TVNGEWFFYEIPIEDNKVLQDIINRYNPQVFVHLAAQAGVRYSITNPAAYIQSNLVGFAN
CCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHH
VLEGCRQNQIPHLIYASSSSVYGGNKNLPFYEEQAVNHPVSLYAATKKSNELMAHTYSHL
HHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
YDLPTTGLRFFTVYGPWGRPDMAPMIFARSILNNEPIQVFNYGKMQRDFTYIDDVVEGII
HCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
RCCFKKASIDDEFNPLVPNPSTSSAPYRIFNIGNSRPTQLTYFIELLEKNLGKKAIKNFQ
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHCCC
PMQPGDVVSTAARMDLLNSWVDYKPITSIENGIKLFSEWYLDYFKNTF
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSKASNETILVTGAAGFIGAALVKALLNLDFKVIGIDNLNDYYSTSLKRSRLTEIEKVS
CCCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
TVNGEWFFYEIPIEDNKVLQDIINRYNPQVFVHLAAQAGVRYSITNPAAYIQSNLVGFAN
CCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHH
VLEGCRQNQIPHLIYASSSSVYGGNKNLPFYEEQAVNHPVSLYAATKKSNELMAHTYSHL
HHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
YDLPTTGLRFFTVYGPWGRPDMAPMIFARSILNNEPIQVFNYGKMQRDFTYIDDVVEGII
HCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
RCCFKKASIDDEFNPLVPNPSTSSAPYRIFNIGNSRPTQLTYFIELLEKNLGKKAIKNFQ
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHCCC
PMQPGDVVSTAARMDLLNSWVDYKPITSIENGIKLFSEWYLDYFKNTF
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]