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The map label for this gene is tgt [H]

Identifier: 124025063

GI number: 124025063

Start: 323737

End: 324867

Strand: Direct

Name: tgt [H]

Synonym: NATL1_03501

Alternate gene names: 124025063

Gene position: 323737-324867 (Clockwise)

Preceding gene: 124025061

Following gene: 124025064

Centisome position: 17.36

GC content: 38.64

Gene sequence:

>1131_bases
TTGAAAAAGCCCTTATTCGATTTCCAAATAAAAAGTAGATGCAAAACGACATTAGCTCGTGTCTGTTCTTTTAAAACTCC
AAATGGAATTGTAAATACTCCAAAATTCATGCCTGTTGGGACTTTAGGAACAGTCAAAGGAGTTACATCTCAGCAACTTG
AGAAAACAGGGGCTGAAATGATTCTTGCAAATACTTTTCATCTTCATCTTCAACCAGGAGAAAAAATAGTTCAAGATGCA
GGGGGACTACATAAATTTATGAGTTGGAATAAACCAATTCTTACTGACTCAGGAGGCTTTCAAGTATTTAGTTTGGCAAA
ATTAAATAAAATAGATGATCAAGGAGTCTCGTTTCAAAGTCCAAGAGATGGTAAACATATCTATTTAACTCCTGAAAAAG
CTATTGAAATTCAAATGGCTTTAGGGTCAGATGTGGCAATGGCTTTTGATCAATGTCCCCCTTACCCAGCAAGCGAATCA
GACGTTGAGGAGGCTTGCAAGAGAACCCATCATTGGTTGGAGAGATGTTTAAATGCTCATAAGAAAAATGATCAAGCAGT
TTTTGGAATAGTTCAAGGTGGTTGCTTCCCTCATTTAAGGGAATTGAGTGCAAAAATTGTCTCAGGATTTGGTTTGCCAG
GGATTGCTATTGGAGGAGTAAGCGTAGGTGAACCCATAAATCAAATGCACAAAATCGTACGAGAAACCTGTCCTTTATTA
CCTCAAGATCGACCTAGATACTTAATGGGAATTGGAACCTTAAAAGAAATGGCCATTGCAGTTGCAAATGGAGTTGACAT
GTTCGATTGTGTAATTCCTACGCGGCTGGGAAGGCATGGAAGCGCATTAGTTAATGGAGAGACATGGAATCTAAGGAATT
CACGTTTTAAAGACGATTACAGCCCATTAGACTCAACATGTACTTGTGAAGCTTGTACGGGATATTCAAGGGCATATATT
CATCATTTAATTCGCAATAAAGAATTACTAGGTCTTACACTTTTGAGTATGCACAATCTTACTCATTTGATACGTTTTAC
AGGTGCTATGAGGCAAGCAATTACTGAAGGTTGTTTTTCAGAGGATTTCGCTCCGTGGCAGAGTGACTCTAAAGCGCGTC
ATACGTGGTAG

Upstream 100 bases:

>100_bases
CCTAGCCAGTTTTTAAAAATCAGAATTACAGCCTCAACTCTTTCTTAACTCTCAAATTAGTTATATAACTAGTCTAAGTT
ATGGAATATTTAAGAAATAA

Downstream 100 bases:

>100_bases
CGTGCGTGCTTGATACGTCCATTATTTCTTAGGGATGGCACTGATTAATTTCGACTTGCTTGCTGAACTTCCAGTTGCAT
ACCAAGCTTTTGCACCAACA

Product: tRNA-guanine transglycosylase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKKPLFDFQIKSRCKTTLARVCSFKTPNGIVNTPKFMPVGTLGTVKGVTSQQLEKTGAEMILANTFHLHLQPGEKIVQDA
GGLHKFMSWNKPILTDSGGFQVFSLAKLNKIDDQGVSFQSPRDGKHIYLTPEKAIEIQMALGSDVAMAFDQCPPYPASES
DVEEACKRTHHWLERCLNAHKKNDQAVFGIVQGGCFPHLRELSAKIVSGFGLPGIAIGGVSVGEPINQMHKIVRETCPLL
PQDRPRYLMGIGTLKEMAIAVANGVDMFDCVIPTRLGRHGSALVNGETWNLRNSRFKDDYSPLDSTCTCEACTGYSRAYI
HHLIRNKELLGLTLLSMHNLTHLIRFTGAMRQAITEGCFSEDFAPWQSDSKARHTW

Sequences:

>Translated_376_residues
MKKPLFDFQIKSRCKTTLARVCSFKTPNGIVNTPKFMPVGTLGTVKGVTSQQLEKTGAEMILANTFHLHLQPGEKIVQDA
GGLHKFMSWNKPILTDSGGFQVFSLAKLNKIDDQGVSFQSPRDGKHIYLTPEKAIEIQMALGSDVAMAFDQCPPYPASES
DVEEACKRTHHWLERCLNAHKKNDQAVFGIVQGGCFPHLRELSAKIVSGFGLPGIAIGGVSVGEPINQMHKIVRETCPLL
PQDRPRYLMGIGTLKEMAIAVANGVDMFDCVIPTRLGRHGSALVNGETWNLRNSRFKDDYSPLDSTCTCEACTGYSRAYI
HHLIRNKELLGLTLLSMHNLTHLIRFTGAMRQAITEGCFSEDFAPWQSDSKARHTW
>Mature_376_residues
MKKPLFDFQIKSRCKTTLARVCSFKTPNGIVNTPKFMPVGTLGTVKGVTSQQLEKTGAEMILANTFHLHLQPGEKIVQDA
GGLHKFMSWNKPILTDSGGFQVFSLAKLNKIDDQGVSFQSPRDGKHIYLTPEKAIEIQMALGSDVAMAFDQCPPYPASES
DVEEACKRTHHWLERCLNAHKKNDQAVFGIVQGGCFPHLRELSAKIVSGFGLPGIAIGGVSVGEPINQMHKIVRETCPLL
PQDRPRYLMGIGTLKEMAIAVANGVDMFDCVIPTRLGRHGSALVNGETWNLRNSRFKDDYSPLDSTCTCEACTGYSRAYI
HHLIRNKELLGLTLLSMHNLTHLIRFTGAMRQAITEGCFSEDFAPWQSDSKARHTW

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=42.5352112676056, Blast_Score=310, Evalue=1e-84,
Organism=Escherichia coli, GI1786607, Length=354, Percent_Identity=47.1751412429379, Blast_Score=345, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17544680, Length=368, Percent_Identity=42.6630434782609, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI25146502, Length=219, Percent_Identity=24.2009132420091, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920510, Length=372, Percent_Identity=42.2043010752688, Blast_Score=311, Evalue=3e-85,
Organism=Drosophila melanogaster, GI21355311, Length=254, Percent_Identity=24.4094488188976, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41672; Mature: 41672

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPLFDFQIKSRCKTTLARVCSFKTPNGIVNTPKFMPVGTLGTVKGVTSQQLEKTGAEM
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCE
ILANTFHLHLQPGEKIVQDAGGLHKFMSWNKPILTDSGGFQVFSLAKLNKIDDQGVSFQS
EEEEEEEEEECCCHHHHHHCCHHHHHHCCCCCEEECCCCCEEEEEHHHCCCCCCCCCCCC
PRDGKHIYLTPEKAIEIQMALGSDVAMAFDQCPPYPASESDVEEACKRTHHWLERCLNAH
CCCCCEEEECCCCCEEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KKNDQAVFGIVQGGCFPHLRELSAKIVSGFGLPGIAIGGVSVGEPINQMHKIVRETCPLL
CCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCC
PQDRPRYLMGIGTLKEMAIAVANGVDMFDCVIPTRLGRHGSALVNGETWNLRNSRFKDDY
CCCCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCEECCCCCCCCCCC
SPLDSTCTCEACTGYSRAYIHHLIRNKELLGLTLLSMHNLTHLIRFTGAMRQAITEGCFS
CCCCCCEEEHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EDFAPWQSDSKARHTW
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKPLFDFQIKSRCKTTLARVCSFKTPNGIVNTPKFMPVGTLGTVKGVTSQQLEKTGAEM
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCE
ILANTFHLHLQPGEKIVQDAGGLHKFMSWNKPILTDSGGFQVFSLAKLNKIDDQGVSFQS
EEEEEEEEEECCCHHHHHHCCHHHHHHCCCCCEEECCCCCEEEEEHHHCCCCCCCCCCCC
PRDGKHIYLTPEKAIEIQMALGSDVAMAFDQCPPYPASESDVEEACKRTHHWLERCLNAH
CCCCCEEEECCCCCEEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KKNDQAVFGIVQGGCFPHLRELSAKIVSGFGLPGIAIGGVSVGEPINQMHKIVRETCPLL
CCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCC
PQDRPRYLMGIGTLKEMAIAVANGVDMFDCVIPTRLGRHGSALVNGETWNLRNSRFKDDY
CCCCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCEECCCCCCCCCCC
SPLDSTCTCEACTGYSRAYIHHLIRNKELLGLTLLSMHNLTHLIRFTGAMRQAITEGCFS
CCCCCCEEEHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EDFAPWQSDSKARHTW
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA