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The map label for this gene is ygcM [C]

Identifier: 124024891

GI number: 124024891

Start: 169855

End: 170781

Strand: Reverse

Name: ygcM [C]

Synonym: NATL1_01781

Alternate gene names: 124024891

Gene position: 170781-169855 (Counterclockwise)

Preceding gene: 124024893

Following gene: 124024890

Centisome position: 9.16

GC content: 37.32

Gene sequence:

>927_bases
ATGGAAGCAATCACATCGAATTTCCCTCACGGAGAGGGGAGAACCTGTGTAATCACAAGGCGAGCCTGCTTTAGCGCAAG
CCACCTTTATAGGCTTCCTGAATTATCTGATGATGATAATTCAGCTTTGTTTGGCCCATGCTCAATAGCTCCTGGCCACG
GACATAATTACGAGTTAATCGTAACCATGGAAGGAGATCTTAATAAATATGGCATGGTCTTAAACCTCTCAGATGTAAAA
TATGCAATAAAAAATGAAGTAACAAGTCAGCTTGACTTTCGTTTTTTAAATGACACATGGCCTGAGTTTGACCTTTCTAA
ACCCGAAGGTTGCTTGCCCACAACTGAAAGTTTAGTTCGTATTATATGGGAGCGCCTTAAATCTCACTTACCCCTAAAAT
CTCTTCGTCTTTACGAAAACCCAAAACTCTGGGCTGACTATCAAGGAAATGCTATGGATGCTTATTTAACAGTTCAAACA
CACTTTTCGGCTGCTCATCGCTTAGCCAGAGAAGACCTACCTCAAACCGAAAACGAAAAAATATTCGGAAAATGTGCTCG
ACCTAATGGACATGGTCACAATTACTTAGTAGATATAACTGTCAAAGGGAAGATTAATCCTAGAACTGGAATGATATGCG
ATTTATCTGCATTAAATAGTTTAATAAATGATTTAGTTGTAGAACCTTTTGATCATACCTTTTTAAATAAAGACATTCCT
TATTTTGCAAATTGTGTACCTACAGCTGAAAATATTGCATTACACATTTCAGATAAATTAACTAATCCAATTAATGCGAT
TGGGGCTAATTTATATAAGATAAAGCTACAGGAAAGTCCTAATAATGCTGCAGAAGTTTATGCAAACCTACCTGAATCAA
CAATCGATACCAAAGACTCTAAGAATCAGGTTGGTTTGCTGAGATAA

Upstream 100 bases:

>100_bases
ACCACCTAACTTTTCTTTAAGAGTTTTAGGGGTTGATTGGACAGCCATAAAGACAGAAGTAACTAAAAGTTATTAAGATG
TATGTTGATGTGAAAAATTT

Downstream 100 bases:

>100_bases
TTGAAAAAAAAATGGGTTAAATTAGTTTTAGCTTCTAGTATTGATGGACGTATCGCATATCCAGAAGGAGGGAAAACACA
ATTAGGTCAATCTGGTGATC

Product: 6-pyruvoyl tetrahydropterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl-Tetrahydropterin Synthase-Like Protein; 6-Pyruvoyl Tetrahydrobiopterin Synthase; 6-Pyruvoyl Tetrahydropterin Synthase Family; 6-Pyruvoyl Tetrahydropterin Synthase Family Protein; Tetrahydrobiopterin Biosynthesis-Like Protein; 6-Pyruvoyltetrahydrobiopterin Synthase; Queuosine Biosynthesis Protein QueD; 6-Pyruvoyltetrahydropterin Synthase Synthase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MEAITSNFPHGEGRTCVITRRACFSASHLYRLPELSDDDNSALFGPCSIAPGHGHNYELIVTMEGDLNKYGMVLNLSDVK
YAIKNEVTSQLDFRFLNDTWPEFDLSKPEGCLPTTESLVRIIWERLKSHLPLKSLRLYENPKLWADYQGNAMDAYLTVQT
HFSAAHRLAREDLPQTENEKIFGKCARPNGHGHNYLVDITVKGKINPRTGMICDLSALNSLINDLVVEPFDHTFLNKDIP
YFANCVPTAENIALHISDKLTNPINAIGANLYKIKLQESPNNAAEVYANLPESTIDTKDSKNQVGLLR

Sequences:

>Translated_308_residues
MEAITSNFPHGEGRTCVITRRACFSASHLYRLPELSDDDNSALFGPCSIAPGHGHNYELIVTMEGDLNKYGMVLNLSDVK
YAIKNEVTSQLDFRFLNDTWPEFDLSKPEGCLPTTESLVRIIWERLKSHLPLKSLRLYENPKLWADYQGNAMDAYLTVQT
HFSAAHRLAREDLPQTENEKIFGKCARPNGHGHNYLVDITVKGKINPRTGMICDLSALNSLINDLVVEPFDHTFLNKDIP
YFANCVPTAENIALHISDKLTNPINAIGANLYKIKLQESPNNAAEVYANLPESTIDTKDSKNQVGLLR
>Mature_308_residues
MEAITSNFPHGEGRTCVITRRACFSASHLYRLPELSDDDNSALFGPCSIAPGHGHNYELIVTMEGDLNKYGMVLNLSDVK
YAIKNEVTSQLDFRFLNDTWPEFDLSKPEGCLPTTESLVRIIWERLKSHLPLKSLRLYENPKLWADYQGNAMDAYLTVQT
HFSAAHRLAREDLPQTENEKIFGKCARPNGHGHNYLVDITVKGKINPRTGMICDLSALNSLINDLVVEPFDHTFLNKDIP
YFANCVPTAENIALHISDKLTNPINAIGANLYKIKLQESPNNAAEVYANLPESTIDTKDSKNQVGLLR

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506331, Length=134, Percent_Identity=44.7761194029851, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI115532224, Length=121, Percent_Identity=41.3223140495868, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI115532226, Length=121, Percent_Identity=41.3223140495868, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19550074, Length=129, Percent_Identity=42.6356589147287, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585378, Length=129, Percent_Identity=42.6356589147287, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 34546; Mature: 34546

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAITSNFPHGEGRTCVITRRACFSASHLYRLPELSDDDNSALFGPCSIAPGHGHNYELI
CCCCCCCCCCCCCCEEEEEEHHHHCHHHHEECCCCCCCCCCEEEECCCCCCCCCCCEEEE
VTMEGDLNKYGMVLNLSDVKYAIKNEVTSQLDFRFLNDTWPEFDLSKPEGCLPTTESLVR
EEECCCCCCCEEEEEEHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCCCHHHHHH
IIWERLKSHLPLKSLRLYENPKLWADYQGNAMDAYLTVQTHFSAAHRLAREDLPQTENEK
HHHHHHHHHCCHHHHEEECCCCEEECCCCCEEEEEEEEEEHHHHHHHHHHHCCCCCCCCC
IFGKCARPNGHGHNYLVDITVKGKINPRTGMICDLSALNSLINDLVVEPFDHTFLNKDIP
EEEEECCCCCCCCEEEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHCCCCCC
YFANCVPTAENIALHISDKLTNPINAIGANLYKIKLQESPNNAAEVYANLPESTIDTKDS
HHHHCCCCCCCEEEEEECCCCCCHHHHCCEEEEEEEECCCCCHHHHEECCCCCCCCCCCC
KNQVGLLR
CCCCCCCC
>Mature Secondary Structure
MEAITSNFPHGEGRTCVITRRACFSASHLYRLPELSDDDNSALFGPCSIAPGHGHNYELI
CCCCCCCCCCCCCCEEEEEEHHHHCHHHHEECCCCCCCCCCEEEECCCCCCCCCCCEEEE
VTMEGDLNKYGMVLNLSDVKYAIKNEVTSQLDFRFLNDTWPEFDLSKPEGCLPTTESLVR
EEECCCCCCCEEEEEEHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCCCHHHHHH
IIWERLKSHLPLKSLRLYENPKLWADYQGNAMDAYLTVQTHFSAAHRLAREDLPQTENEK
HHHHHHHHHCCHHHHEEECCCCEEECCCCCEEEEEEEEEEHHHHHHHHHHHCCCCCCCCC
IFGKCARPNGHGHNYLVDITVKGKINPRTGMICDLSALNSLINDLVVEPFDHTFLNKDIP
EEEEECCCCCCCCEEEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHCCCCCC
YFANCVPTAENIALHISDKLTNPINAIGANLYKIKLQESPNNAAEVYANLPESTIDTKDS
HHHHCCCCCCCEEEEEECCCCCCHHHHCCEEEEEEEECCCCCHHHHEECCCCCCCCCCCC
KNQVGLLR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA