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The map label for this gene is dnaN [H]

Identifier: 124024713

GI number: 124024713

Start: 188

End: 1348

Strand: Direct

Name: dnaN [H]

Synonym: NATL1_00001

Alternate gene names: 124024713

Gene position: 188-1348 (Clockwise)

Preceding gene: NA

Following gene: 124024714

Centisome position: 0.01

GC content: 31.78

Gene sequence:

>1161_bases
ATGAAATTAAATTGTTCTCAATCTGAATTAAATAATGCTCTTCAATTGGTTAGTAGAGCTGTTTCTAATCGACCGACGCA
CCCTGTATTAGCTAATGTTTTGCTCGCTGCTGATGAGGTAACTGGAAAACTTCGCCTAACTGGTTTTGACTTAAGTTTAG
GAATACAAACTTCAATATCTGCTTCTATAGAGAGCAGTGGTGCCATAACTTTGCCTGCTAAGTTATTTGGTGAAATTGTT
TCGAAATTATCTAACGACTCACCTCTAGTTCTGACATCAGATGAAACTAGTCAACAAGTCGAGTTGACTAGTAAAAGTGG
AACGTATCAAGTAAGAGGGATGGTGTCTGATGATTTTCCTGATTTACCTTTGGTAGAAAGCGGTAATTCATTTAAATTGA
ATCCATTATTATTATCTAATGCAATTAAATCAACTTTATTTGCAAGCAGTACTGATGAGGCTAAACAATTACTTACTGGT
GTTAATCTTACTTTTGACGGATATGGGATTGAATCTGCAGCAACTGACGGACATAGATTAGCTGTTCTTAATTTAAATAA
TATATTATCTGATTCAAAAGAGATAGATAGTTCTACTAAATTCGAAAAATTTTCTATTACATTACCTTCCAAATCTTTAA
GAGAAGTGGAAAAATTTTTAAGTACTTGTGATATTAGTCAGCCAATTAATTTTTTTGTTGATAAAGGACAAGTAGTTTTT
ATATCAGTAGAAGATATTATAACTATAAGAGCTTTAGAAGGTTCTTATCCAAATTATTCGCAATTATTACCTGATACTTT
TGAAAATGAATTAGAGTTTGATAGGAAAGAATTTATATCTTCATTAGAACGAATTGCTGTTTTAGCAGATCAGCATAATA
ATGTTATAAAGGTAACGACTGACGGCTCTGCAAATTTAATCAAAATTAGTACGGATGCCCAAGATATTGGAACAGGTTAT
GAGTCACTACCTGTCTCTTATAAAGGGGATTCATTTCAAATAGCATTTAATGTTAGATATTTATTAGATGGACTTAAAGT
CATAGATTCAAACTTGATTAAATTAAGTTGTAATTCTCCAACTACACCTGCGGTTTTCTCACCTATTAATAGTGACGTTA
ATTTCACTTATCTAGTAATGCCCATACAAATACGAAATTAA

Upstream 100 bases:

>100_bases
GTTAAATAGAAGTAAAAAGTTTTGTTTTTTTTAACGGTATTGCTTTTTGGAAATAGCTATGAAATTCTTGTATCAATGGA
TTTTTATTTGAAATAACAGG

Downstream 100 bases:

>100_bases
TTTTGGCTGTCCCTAAAAAATTACTACTTAGTGATTTACTAAAACATAATGTTCGTTGTGATAGTGGAATGGATCACGGT
CCAGTTATTACTCCATGGAT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKLNCSQSELNNALQLVSRAVSNRPTHPVLANVLLAADEVTGKLRLTGFDLSLGIQTSISASIESSGAITLPAKLFGEIV
SKLSNDSPLVLTSDETSQQVELTSKSGTYQVRGMVSDDFPDLPLVESGNSFKLNPLLLSNAIKSTLFASSTDEAKQLLTG
VNLTFDGYGIESAATDGHRLAVLNLNNILSDSKEIDSSTKFEKFSITLPSKSLREVEKFLSTCDISQPINFFVDKGQVVF
ISVEDIITIRALEGSYPNYSQLLPDTFENELEFDRKEFISSLERIAVLADQHNNVIKVTTDGSANLIKISTDAQDIGTGY
ESLPVSYKGDSFQIAFNVRYLLDGLKVIDSNLIKLSCNSPTTPAVFSPINSDVNFTYLVMPIQIRN

Sequences:

>Translated_386_residues
MKLNCSQSELNNALQLVSRAVSNRPTHPVLANVLLAADEVTGKLRLTGFDLSLGIQTSISASIESSGAITLPAKLFGEIV
SKLSNDSPLVLTSDETSQQVELTSKSGTYQVRGMVSDDFPDLPLVESGNSFKLNPLLLSNAIKSTLFASSTDEAKQLLTG
VNLTFDGYGIESAATDGHRLAVLNLNNILSDSKEIDSSTKFEKFSITLPSKSLREVEKFLSTCDISQPINFFVDKGQVVF
ISVEDIITIRALEGSYPNYSQLLPDTFENELEFDRKEFISSLERIAVLADQHNNVIKVTTDGSANLIKISTDAQDIGTGY
ESLPVSYKGDSFQIAFNVRYLLDGLKVIDSNLIKLSCNSPTTPAVFSPINSDVNFTYLVMPIQIRN
>Mature_386_residues
MKLNCSQSELNNALQLVSRAVSNRPTHPVLANVLLAADEVTGKLRLTGFDLSLGIQTSISASIESSGAITLPAKLFGEIV
SKLSNDSPLVLTSDETSQQVELTSKSGTYQVRGMVSDDFPDLPLVESGNSFKLNPLLLSNAIKSTLFASSTDEAKQLLTG
VNLTFDGYGIESAATDGHRLAVLNLNNILSDSKEIDSSTKFEKFSITLPSKSLREVEKFLSTCDISQPINFFVDKGQVVF
ISVEDIITIRALEGSYPNYSQLLPDTFENELEFDRKEFISSLERIAVLADQHNNVIKVTTDGSANLIKISTDAQDIGTGY
ESLPVSYKGDSFQIAFNVRYLLDGLKVIDSNLIKLSCNSPTTPAVFSPINSDVNFTYLVMPIQIRN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=385, Percent_Identity=24.9350649350649, Blast_Score=118, Evalue=6e-28,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42054; Mature: 42054

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNCSQSELNNALQLVSRAVSNRPTHPVLANVLLAADEVTGKLRLTGFDLSLGIQTSIS
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCEEEEE
ASIESSGAITLPAKLFGEIVSKLSNDSPLVLTSDETSQQVELTSKSGTYQVRGMVSDDFP
CCCCCCCCEEECHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
DLPLVESGNSFKLNPLLLSNAIKSTLFASSTDEAKQLLTGVNLTFDGYGIESAATDGHRL
CCCEEECCCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCCCCCCCCEE
AVLNLNNILSDSKEIDSSTKFEKFSITLPSKSLREVEKFLSTCDISQPINFFVDKGQVVF
EEEECHHHHCCCHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEE
ISVEDIITIRALEGSYPNYSQLLPDTFENELEFDRKEFISSLERIAVLADQHNNVIKVTT
EEEHHEEEEEEECCCCCCHHHHCCHHHHHHHCCCHHHHHHHHHHHHHEECCCCCEEEEEE
DGSANLIKISTDAQDIGTGYESLPVSYKGDSFQIAFNVRYLLDGLKVIDSNLIKLSCNSP
CCCCCEEEEECCHHHHCCCHHHCCEEECCCCEEEEEEHHHHHHHHHHCCCCEEEEECCCC
TTPAVFSPINSDVNFTYLVMPIQIRN
CCCCEECCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MKLNCSQSELNNALQLVSRAVSNRPTHPVLANVLLAADEVTGKLRLTGFDLSLGIQTSIS
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCEEEEE
ASIESSGAITLPAKLFGEIVSKLSNDSPLVLTSDETSQQVELTSKSGTYQVRGMVSDDFP
CCCCCCCCEEECHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
DLPLVESGNSFKLNPLLLSNAIKSTLFASSTDEAKQLLTGVNLTFDGYGIESAATDGHRL
CCCEEECCCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCCCCCCCCEE
AVLNLNNILSDSKEIDSSTKFEKFSITLPSKSLREVEKFLSTCDISQPINFFVDKGQVVF
EEEECHHHHCCCHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEE
ISVEDIITIRALEGSYPNYSQLLPDTFENELEFDRKEFISSLERIAVLADQHNNVIKVTT
EEEHHEEEEEEECCCCCCHHHHCCHHHHHHHCCCHHHHHHHHHHHHHEECCCCCEEEEEE
DGSANLIKISTDAQDIGTGYESLPVSYKGDSFQIAFNVRYLLDGLKVIDSNLIKLSCNSP
CCCCCEEEEECCHHHHCCCHHHCCEEECCCCEEEEEEHHHHHHHHHHCCCCEEEEECCCC
TTPAVFSPINSDVNFTYLVMPIQIRN
CCCCEECCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8654968 [H]