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The map label for this gene is purU [H]

Identifier: 124024690

GI number: 124024690

Start: 2656454

End: 2657344

Strand: Reverse

Name: purU [H]

Synonym: P9303_30021

Alternate gene names: 124024690

Gene position: 2657344-2656454 (Counterclockwise)

Preceding gene: 124024692

Following gene: 124024689

Centisome position: 99.06

GC content: 52.08

Gene sequence:

>891_bases
GTGAGCTCCCCTCTTGGTGCTGTATGGAATCCTTACGGTTACAAGCTTCGTGTGATTCTGCAGCTCATCTGTCCAGATCG
GCCTGCTTTGGTGAGTGAGTTGTCGGGATGGGTGGCTGTTAATGGAGGCAATATTCTTCATGCTGATCACCATACGGATG
TTGGGGCTGGACTGTTTCTGAGCAGGATCGAATTTGGAATTGAGGGCTTCGGATTGCCTAGAGAGGCGATAGCACCAGCT
GTGAATGCCCTGGCAGATCGCCTCGGGGGTCAGGCGCAATTGCATTTTTCGGATGAGATCCCTCGGGTGGCAATCTTCGC
CAGCAAGCAGAGTCACTGTCTGTTGGATTTGCTTTGGCGCACTCGCAGTGGGGAGTTGCCGATGCAGGTGCCGCTTGTGA
TTGCAAACCACTCTCAATTGGAGCCTTTATGCAGGGAGTTTGGTGTTTGTTTTGAGTGTGTTCCTATGACGCCTGCTAGC
AAGCCTGAGGCGGAACAAACCATGCTGGATTTGTTGGCTGAGCATCGGATTGAGCTGGTTGTGTTGGCCAAGTACATGCA
GGTCCTTAGTGGTGCTTTTCTAGAGCGTTTCCCCACCGTGATTAATATTCACCATTCCTTCCTGCCGGCTTTTAAGGGGG
CACAGCCCTATCACAGGGCCTGGGATCGAGGGGTGAAAGTGATTGGGGCTACCGCCCATTACGTCACTGAAGATCTGGAT
GACGGGCCGATTATTGAGCAGACCATCGAGCATGTAAACCATCGTGATGAGGTGGAAGATTTGATTCGTAAAGGACGTGA
TACGGAACGTCTCGCTTTGGCAAGGGCCTTGAGATTGCATCTTTGTCGCCAGGTGATGGTCTATCGCGGTAGAACCGCTG
TTTTTGCATGA

Upstream 100 bases:

>100_bases
AAGCAGTCATTCTTGATATTGACGAGCAATGCATCTCACTTTGAGGTGTTCTTGGTGCGGACGTTCGTCAAGACAGAATG
AGTTTTTGCAATCAGGTTGA

Downstream 100 bases:

>100_bases
CGGTGTTGGCTTGGATGGCTCGATGTCGCCCTGCTGCTGCGACCTCCATGGGTTGGCTTGTTTTCCAGTGGGGGCTTTTT
TTGTTGCCTTCTAGTGCATT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSSPLGAVWNPYGYKLRVILQLICPDRPALVSELSGWVAVNGGNILHADHHTDVGAGLFLSRIEFGIEGFGLPREAIAPA
VNALADRLGGQAQLHFSDEIPRVAIFASKQSHCLLDLLWRTRSGELPMQVPLVIANHSQLEPLCREFGVCFECVPMTPAS
KPEAEQTMLDLLAEHRIELVVLAKYMQVLSGAFLERFPTVINIHHSFLPAFKGAQPYHRAWDRGVKVIGATAHYVTEDLD
DGPIIEQTIEHVNHRDEVEDLIRKGRDTERLALARALRLHLCRQVMVYRGRTAVFA

Sequences:

>Translated_296_residues
MSSPLGAVWNPYGYKLRVILQLICPDRPALVSELSGWVAVNGGNILHADHHTDVGAGLFLSRIEFGIEGFGLPREAIAPA
VNALADRLGGQAQLHFSDEIPRVAIFASKQSHCLLDLLWRTRSGELPMQVPLVIANHSQLEPLCREFGVCFECVPMTPAS
KPEAEQTMLDLLAEHRIELVVLAKYMQVLSGAFLERFPTVINIHHSFLPAFKGAQPYHRAWDRGVKVIGATAHYVTEDLD
DGPIIEQTIEHVNHRDEVEDLIRKGRDTERLALARALRLHLCRQVMVYRGRTAVFA
>Mature_295_residues
SSPLGAVWNPYGYKLRVILQLICPDRPALVSELSGWVAVNGGNILHADHHTDVGAGLFLSRIEFGIEGFGLPREAIAPAV
NALADRLGGQAQLHFSDEIPRVAIFASKQSHCLLDLLWRTRSGELPMQVPLVIANHSQLEPLCREFGVCFECVPMTPASK
PEAEQTMLDLLAEHRIELVVLAKYMQVLSGAFLERFPTVINIHHSFLPAFKGAQPYHRAWDRGVKVIGATAHYVTEDLDD
GPIIEQTIEHVNHRDEVEDLIRKGRDTERLALARALRLHLCRQVMVYRGRTAVFA

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=31.5789473684211, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=31.5789473684211, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=31.5789473684211, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1787483, Length=280, Percent_Identity=41.0714285714286, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17567511, Length=166, Percent_Identity=35.5421686746988, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24582400, Length=195, Percent_Identity=28.7179487179487, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33000; Mature: 32868

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPLGAVWNPYGYKLRVILQLICPDRPALVSELSGWVAVNGGNILHADHHTDVGAGLFL
CCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCEEECCCCCCCHHHHHH
SRIEFGIEGFGLPREAIAPAVNALADRLGGQAQLHFSDEIPRVAIFASKQSHCLLDLLWR
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHH
TRSGELPMQVPLVIANHSQLEPLCREFGVCFECVPMTPASKPEAEQTMLDLLAEHRIELV
CCCCCCCEEEEEEEECCHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
VLAKYMQVLSGAFLERFPTVINIHHSFLPAFKGAQPYHRAWDRGVKVIGATAHYVTEDLD
HHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHCCCCHHHHHHHCCCEEEECHHHHHHHCCC
DGPIIEQTIEHVNHRDEVEDLIRKGRDTERLALARALRLHLCRQVMVYRGRTAVFA
CCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
SSPLGAVWNPYGYKLRVILQLICPDRPALVSELSGWVAVNGGNILHADHHTDVGAGLFL
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCEEECCCCCCCHHHHHH
SRIEFGIEGFGLPREAIAPAVNALADRLGGQAQLHFSDEIPRVAIFASKQSHCLLDLLWR
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHH
TRSGELPMQVPLVIANHSQLEPLCREFGVCFECVPMTPASKPEAEQTMLDLLAEHRIELV
CCCCCCCEEEEEEEECCHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
VLAKYMQVLSGAFLERFPTVINIHHSFLPAFKGAQPYHRAWDRGVKVIGATAHYVTEDLD
HHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHCCCCHHHHHHHCCCEEEECHHHHHHHCCC
DGPIIEQTIEHVNHRDEVEDLIRKGRDTERLALARALRLHLCRQVMVYRGRTAVFA
CCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]