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The map label for this gene is mraW

Identifier: 124024411

GI number: 124024411

Start: 2399885

End: 2400817

Strand: Reverse

Name: mraW

Synonym: P9303_27231

Alternate gene names: 124024411

Gene position: 2400817-2399885 (Counterclockwise)

Preceding gene: 124024414

Following gene: 124024410

Centisome position: 89.49

GC content: 58.41

Gene sequence:

>933_bases
ATGCCCGATTCGTCCATTAACTCGGATTCAGGTTTCAAGCATCTGTCCGTAATGGCAGATGCCGTGTCGCAGCCTGTCGC
TGCTTTGCCTGCAGAGCTGCTCGATGGCGGGTTGATGATTGATGCCACCCTTGGCGGCGGCGGCCACAGTGCTCTGTTGC
TGGAACGTCATCCAGATCTCCGTCTGATCGGTTTGGACCAAGACCCCACGGCTCGTGCGGCGGCGGCTGAGCGACTGGCG
TTATTTGGAGATCGCGTCAAGATCGTGGCGACTAATTTTGTCGATTTCAGCCCTGCTGAGCCGGCAGTGGTGGTGATGGC
TGATTTGGGAGTGAGCAGTCCCCAACTGGATGTGGCGAAAAGGGGTTTTAGCTTTCGCCTCGATGGTCCGCTCGACATGC
GCATGAACCCCCAGGTGGGCGAAACGGCGGCTGAGCTGATTGGGCGTCTCGAAGAAACCGAACTGGCAGATCTCATCTAT
GCCTATGGCGAGGAGCGCTTGTCCCGTCGCATCGCCCGGCGTATTAAGCACGATCTGGCAGAGCAAGGACCCTATGCCGG
CACCGCCGCTTTGGCTTATGCGGTGGCTGGTTGCTATCCGCCGAAGGCCCGTCGTGGTCGTATCCATCCAGCCACCCGCA
CTTTTCAGGCCCTGCGCATTGCTGTGAATGATGAATTGGATGCCTTAGATCGCTTCTTGCAAAAGGCCCCTGACTGGTTA
GTACCAGGTGGTTTGCTCGCAGTGATCAGCTTTCATTCCCTTGAAGACCGTCGCGTCAAGACCGCTTTCTTGCGTGATCA
GCGCCTGGAGCGGCTAACCCGCAAGCCTTTGGTGGCCAGCGAGATGGAGATCGCTGCCAATCCACGCAGCCGTAGTGCAA
AGTGTCGGTTCGCGCGCCGTTTGCCTCAAGCGATGACTGCCGATGCCCTCTGA

Upstream 100 bases:

>100_bases
ATGGGGGCCGAAGTTGACGACCATTGGCTCCGTACGCGTTTCCAGCTGCGACATGGGGCCTTGAGTTGCAGAGCGTTAGC
GGGATCTTAGGAAGGAACCC

Downstream 100 bases:

>100_bases
TCCAATCTGGTGGTGGGCTTTTGGAGTGCAACTGTTAGCTCTGCCTGGAGCCTTGTTGCCGGTGCTACCGAGTTTGTTGT
GGTTGCCACTCGGTGCTGGG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MPDSSINSDSGFKHLSVMADAVSQPVAALPAELLDGGLMIDATLGGGGHSALLLERHPDLRLIGLDQDPTARAAAAERLA
LFGDRVKIVATNFVDFSPAEPAVVVMADLGVSSPQLDVAKRGFSFRLDGPLDMRMNPQVGETAAELIGRLEETELADLIY
AYGEERLSRRIARRIKHDLAEQGPYAGTAALAYAVAGCYPPKARRGRIHPATRTFQALRIAVNDELDALDRFLQKAPDWL
VPGGLLAVISFHSLEDRRVKTAFLRDQRLERLTRKPLVASEMEIAANPRSRSAKCRFARRLPQAMTADAL

Sequences:

>Translated_310_residues
MPDSSINSDSGFKHLSVMADAVSQPVAALPAELLDGGLMIDATLGGGGHSALLLERHPDLRLIGLDQDPTARAAAAERLA
LFGDRVKIVATNFVDFSPAEPAVVVMADLGVSSPQLDVAKRGFSFRLDGPLDMRMNPQVGETAAELIGRLEETELADLIY
AYGEERLSRRIARRIKHDLAEQGPYAGTAALAYAVAGCYPPKARRGRIHPATRTFQALRIAVNDELDALDRFLQKAPDWL
VPGGLLAVISFHSLEDRRVKTAFLRDQRLERLTRKPLVASEMEIAANPRSRSAKCRFARRLPQAMTADAL
>Mature_309_residues
PDSSINSDSGFKHLSVMADAVSQPVAALPAELLDGGLMIDATLGGGGHSALLLERHPDLRLIGLDQDPTARAAAAERLAL
FGDRVKIVATNFVDFSPAEPAVVVMADLGVSSPQLDVAKRGFSFRLDGPLDMRMNPQVGETAAELIGRLEETELADLIYA
YGEERLSRRIARRIKHDLAEQGPYAGTAALAYAVAGCYPPKARRGRIHPATRTFQALRIAVNDELDALDRFLQKAPDWLV
PGGLLAVISFHSLEDRRVKTAFLRDQRLERLTRKPLVASEMEIAANPRSRSAKCRFARRLPQAMTADAL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=346, Percent_Identity=35.8381502890173, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI165377202, Length=177, Percent_Identity=37.2881355932203, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1786270, Length=318, Percent_Identity=40.8805031446541, Blast_Score=187, Evalue=7e-49,
Organism=Drosophila melanogaster, GI62472493, Length=335, Percent_Identity=31.9402985074627, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28571637, Length=335, Percent_Identity=31.9402985074627, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_PROM3 (A2CD93)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001018718.1
- ProteinModelPortal:   A2CD93
- STRING:   A2CD93
- GeneID:   4778810
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_27231
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 33740; Mature: 33609

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: BINDING 66-66 BINDING 93-93 BINDING 108-108 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDSSINSDSGFKHLSVMADAVSQPVAALPAELLDGGLMIDATLGGGGHSALLLERHPDL
CCCCCCCCCCCHHHHHHHHHHHHCCHHHCCHHHHCCCEEEEEEECCCCCEEEEEECCCCE
RLIGLDQDPTARAAAAERLALFGDRVKIVATNFVDFSPAEPAVVVMADLGVSSPQLDVAK
EEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHH
RGFSFRLDGPLDMRMNPQVGETAAELIGRLEETELADLIYAYGEERLSRRIARRIKHDLA
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQGPYAGTAALAYAVAGCYPPKARRGRIHPATRTFQALRIAVNDELDALDRFLQKAPDWL
HCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
VPGGLLAVISFHSLEDRRVKTAFLRDQRLERLTRKPLVASEMEIAANPRSRSAKCRFARR
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHH
LPQAMTADAL
CHHHHHCCCC
>Mature Secondary Structure 
PDSSINSDSGFKHLSVMADAVSQPVAALPAELLDGGLMIDATLGGGGHSALLLERHPDL
CCCCCCCCCCHHHHHHHHHHHHCCHHHCCHHHHCCCEEEEEEECCCCCEEEEEECCCCE
RLIGLDQDPTARAAAAERLALFGDRVKIVATNFVDFSPAEPAVVVMADLGVSSPQLDVAK
EEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHH
RGFSFRLDGPLDMRMNPQVGETAAELIGRLEETELADLIYAYGEERLSRRIARRIKHDLA
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQGPYAGTAALAYAVAGCYPPKARRGRIHPATRTFQALRIAVNDELDALDRFLQKAPDWL
HCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
VPGGLLAVISFHSLEDRRVKTAFLRDQRLERLTRKPLVASEMEIAANPRSRSAKCRFARR
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHH
LPQAMTADAL
CHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA