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The map label for this gene is kefB [H]

Identifier: 124024282

GI number: 124024282

Start: 2288180

End: 2289553

Strand: Reverse

Name: kefB [H]

Synonym: P9303_25931

Alternate gene names: 124024282

Gene position: 2289553-2288180 (Counterclockwise)

Preceding gene: 124024283

Following gene: 124024281

Centisome position: 85.35

GC content: 54.08

Gene sequence:

>1374_bases
ATGGTTTTGCATTCGGTGTTGTCCGTGCTGAGCAGCCACGACGTGGAGGTGGCTGAAACATTGATCGGTGTGCTGAATTT
CCTGCTGATTTTTGTGGCAGCACGCACTCTTGCTGAGCTTTTGGTGCGTTTGCATCTACCGACGATCGTCGGCGAACTGC
TGGCAGGGGTTCTGATCGGGGCCTCAGGTTTGCATCTTTTGGTTCCGCCGAGTGCGCATGTTCAGTTGAACGAAGGTTTG
GTCAGCCTGATCAGCGCCTTGGCCTCGATCCCTCCTGAGGCCGTCCCAGACCTCTATTACGAAACTTTCCCCTCTCTGCA
AGCGGTTGCCACGCTCGGTTTGTATGCCCTGCTTTTCCTCACTGGATTGGAAAGTGAGTTGGAAGAGTTGGTTGCGGTAG
GAGTCCAGGCCTTCACAGTTGCCATGGCTGGCGTGGTGTTGCCTTTTGCTCTAGGCACCTTTGGTTTGATGGCTCTGTTT
CAGGTGGAATTGATCCCGGCGATTTTTGCTGGTGCTTCGATGACTGCTACCAGTATCGGCATCACTGCCAGTGTGTTTGG
CGAACTCGGTTATCTCAAAACGCGAGAGGGCCAGATCGTGATTGGTGCGGCTGTGCTTGATGACATCCTTGGCATTGTGA
TCTTGGCCGTTGTTGTTGCCTTGGCCACAGGCGGGAGCCTCGAGATTGCTCCGATTGTGAAACTTGTTGCAGCAGCGACA
GTGTTTGTGGTCTTAGCAATTGCGCTTAGTCGTACTGCGGCGCCGGCGTTTGATTGGGTGATCGATCGGCTTAAGGCGCC
TGGGGCAGTTGTTGTGGCCTCATTTGTGATCTTGGTGTTCTGTTGCTTTGTCGCTACAGCGATTGGTTTGGAGGCCGCTT
TGGGAGCATTCGCTGCTGGATTGATCCTCAGCAGCTCGAAGAACAATCGCGCAATTCAGCAGTCGGTGCTGCCGATTGTG
ACTCTCTTCGCCACGATTTTCTTTGTTTTGGTTGGCGCTGGCATGGATCTTTCAGTGATCAACCCTTTAGATCCTGCGAG
TCGCTCTGCACTCGTTGTAGCTGGCTTCCTTTTGGTGGTAGCGATTGTGGGCAAGATTGCAGCCGGTTGGGCTTTCTGGA
TCGACAAACCCACCAATCGCTTGGTGGTTGGGCTTGGCATGATGCCTCGCGGCGAAGTCGGTTTGATTTTTCTTGGCCTT
GGTACAAGCGCCGGGCTGCTTTCTCCGTCTTTAGAAGCAGCAGTTCTATTGATGGTGATTGGCACCACCTTTCTTGCGCC
GTTGTTGCTTCGCTTGGTGCTTCGAGACAAACCGCCTGGAGGCGGCAACGTCATCCCTGATGACGTTGCCGCCAATCCTG
TCGGGCTGGTTTAG

Upstream 100 bases:

>100_bases
CATCAACACTTGCAGCTTCCAATCAAGCTTCGTTTTGCGCACACCAAAGCAGGATTTGCCTTTCCTTTAATCTCGCTCAC
AAAGACGGAAATCCGGCTCC

Downstream 100 bases:

>100_bases
TGCAGCTTCTTTGCTTGCACAGGCCTCAAAAGTAAAAGAATTGCCTACCATCACCTTTCGCCACCGTCGCCGTTGTGACG
TCAGCTCCATCTGCCGTGCT

Product: Na+/H+ antiporter, CPA2 family

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MVLHSVLSVLSSHDVEVAETLIGVLNFLLIFVAARTLAELLVRLHLPTIVGELLAGVLIGASGLHLLVPPSAHVQLNEGL
VSLISALASIPPEAVPDLYYETFPSLQAVATLGLYALLFLTGLESELEELVAVGVQAFTVAMAGVVLPFALGTFGLMALF
QVELIPAIFAGASMTATSIGITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALATGGSLEIAPIVKLVAAAT
VFVVLAIALSRTAAPAFDWVIDRLKAPGAVVVASFVILVFCCFVATAIGLEAALGAFAAGLILSSSKNNRAIQQSVLPIV
TLFATIFFVLVGAGMDLSVINPLDPASRSALVVAGFLLVVAIVGKIAAGWAFWIDKPTNRLVVGLGMMPRGEVGLIFLGL
GTSAGLLSPSLEAAVLLMVIGTTFLAPLLLRLVLRDKPPGGGNVIPDDVAANPVGLV

Sequences:

>Translated_457_residues
MVLHSVLSVLSSHDVEVAETLIGVLNFLLIFVAARTLAELLVRLHLPTIVGELLAGVLIGASGLHLLVPPSAHVQLNEGL
VSLISALASIPPEAVPDLYYETFPSLQAVATLGLYALLFLTGLESELEELVAVGVQAFTVAMAGVVLPFALGTFGLMALF
QVELIPAIFAGASMTATSIGITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALATGGSLEIAPIVKLVAAAT
VFVVLAIALSRTAAPAFDWVIDRLKAPGAVVVASFVILVFCCFVATAIGLEAALGAFAAGLILSSSKNNRAIQQSVLPIV
TLFATIFFVLVGAGMDLSVINPLDPASRSALVVAGFLLVVAIVGKIAAGWAFWIDKPTNRLVVGLGMMPRGEVGLIFLGL
GTSAGLLSPSLEAAVLLMVIGTTFLAPLLLRLVLRDKPPGGGNVIPDDVAANPVGLV
>Mature_457_residues
MVLHSVLSVLSSHDVEVAETLIGVLNFLLIFVAARTLAELLVRLHLPTIVGELLAGVLIGASGLHLLVPPSAHVQLNEGL
VSLISALASIPPEAVPDLYYETFPSLQAVATLGLYALLFLTGLESELEELVAVGVQAFTVAMAGVVLPFALGTFGLMALF
QVELIPAIFAGASMTATSIGITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALATGGSLEIAPIVKLVAAAT
VFVVLAIALSRTAAPAFDWVIDRLKAPGAVVVASFVILVFCCFVATAIGLEAALGAFAAGLILSSSKNNRAIQQSVLPIV
TLFATIFFVLVGAGMDLSVINPLDPASRSALVVAGFLLVVAIVGKIAAGWAFWIDKPTNRLVVGLGMMPRGEVGLIFLGL
GTSAGLLSPSLEAAVLLMVIGTTFLAPLLLRLVLRDKPPGGGNVIPDDVAANPVGLV

Specific function: This is a Na(+)/H(+) antiporter. It can mediate sodium uptake when a transmembrane pH gradient is applied [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322367, Length=367, Percent_Identity=23.433242506812, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771 [H]

Pfam domain/function: PF00999 Na_H_Exchanger [H]

EC number: NA

Molecular weight: Translated: 46936; Mature: 46936

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLHSVLSVLSSHDVEVAETLIGVLNFLLIFVAARTLAELLVRLHLPTIVGELLAGVLIG
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASGLHLLVPPSAHVQLNEGLVSLISALASIPPEAVPDLYYETFPSLQAVATLGLYALLFL
CCCCEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TGLESELEELVAVGVQAFTVAMAGVVLPFALGTFGLMALFQVELIPAIFAGASMTATSIG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALATGGSLEIAPIVKLVAAAT
HHHHHHHHHCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VFVVLAIALSRTAAPAFDWVIDRLKAPGAVVVASFVILVFCCFVATAIGLEAALGAFAAG
HHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILSSSKNNRAIQQSVLPIVTLFATIFFVLVGAGMDLSVINPLDPASRSALVVAGFLLVV
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHH
AIVGKIAAGWAFWIDKPTNRLVVGLGMMPRGEVGLIFLGLGTSAGLLSPSLEAAVLLMVI
HHHHHHHHCHHEEECCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
GTTFLAPLLLRLVLRDKPPGGGNVIPDDVAANPVGLV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVLHSVLSVLSSHDVEVAETLIGVLNFLLIFVAARTLAELLVRLHLPTIVGELLAGVLIG
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASGLHLLVPPSAHVQLNEGLVSLISALASIPPEAVPDLYYETFPSLQAVATLGLYALLFL
CCCCEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TGLESELEELVAVGVQAFTVAMAGVVLPFALGTFGLMALFQVELIPAIFAGASMTATSIG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALATGGSLEIAPIVKLVAAAT
HHHHHHHHHCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VFVVLAIALSRTAAPAFDWVIDRLKAPGAVVVASFVILVFCCFVATAIGLEAALGAFAAG
HHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILSSSKNNRAIQQSVLPIVTLFATIFFVLVGAGMDLSVINPLDPASRSALVVAGFLLVV
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHH
AIVGKIAAGWAFWIDKPTNRLVVGLGMMPRGEVGLIFLGLGTSAGLLSPSLEAAVLLMVI
HHHHHHHHCHHEEECCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
GTTFLAPLLLRLVLRDKPPGGGNVIPDDVAANPVGLV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312090; 1325937 [H]