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The map label for this gene is 124024200

Identifier: 124024200

GI number: 124024200

Start: 2207734

End: 2209257

Strand: Reverse

Name: 124024200

Synonym: P9303_25111

Alternate gene names: NA

Gene position: 2209257-2207734 (Counterclockwise)

Preceding gene: 124024201

Following gene: 124024198

Centisome position: 82.35

GC content: 52.95

Gene sequence:

>1524_bases
TTGATCAGCCTTGCAGTGAGCACAGTGAGCGATAGCCCTTCAACAGTGTCCGCGACCTCAATAGATAGGCCCGGCGGGTT
TGATCGGGACGACTGGGCAAGTGGTTATTGCAATGTTGAGCAAGAGCTCAATGCTGTGTCTTTACGTCCCCTGAGGGGAC
AGATTCCAGCTGAACTGCAAGGCAGCCACTATCGCAACGGTCCAGGCAGGTTTGAACGCGATGGGCAATGGGTGCATCAC
CCCTTTGATGGCGACGGCATGATCACTGCTGTGCGATTCAGCGATAGCAAGGCCACGCTGAGTAATCGCTTTGTGCGAAC
GAAATACTGGCAGGAAGAGGAAAAGGCTGGTCGCTTCCTCTATCGGGGGGTGTTTGGCACGCAGAAGCGTGGTGGGGCGA
AGGCGAATGCTTTTGACGTGCGTTTCAAGAACATTGCCAATACCCAAGTGGTTCAACTTGGTGATCAGTTACTGGCTCTT
TGGGAGGCGGCTGGTCCGCACGCGCTTGACCCTGTCAGCTTGGAAACTCATGGGCTTTCGAGTCTCAATGGGGTGCTGAA
GCCTGGAGAGGCTTTTAGCGCTCATCCCCGCTTCGATCCTGGCCACCATGGCGAACCTCGCATGGTCACCTTTGGTGTGA
ATACAGGGCCGACTAGCAAGGTCCGGCTGATGGAGTTTGCCACTCAGGGGCCAGATGCGGGACGACTCATCAGTGATCGC
ACAGACAGTTTTAAGGGTTTTGCTTTTCTGCATGACTTCGCGATTACTCCCAACTGGACGCTCTTTTTGCAGAATGCGAT
CAACTTCAATCCGTTGCCGTTTGTGCTGGGTCAGAAGGGTGCGGCGCAATGTTTAACGTCTAAGCCTGGAGGCCAGGCAA
AGTTTTGGTTGATTCCTCGTGATTGCGGCACTTTTGCTGGGCAGCCGCCGCGCATCTTTGATGCTCCCGATGGTTTTGTT
TTTCACCATCTCAATGCTTGGGAGGAGGGGGATGAGTTGGTTTTGGAGAGCATTTACTACAACGATTACCCCTCGATTGA
TCCCGGCGAGGACTTCCTCTCGATGGACTTTGACTTTTTCCCTGAGGGTCGATTAGAGCAGTGCCGCATCAATCTTTTGG
GGAAAGGAATTGAAACCAAGCGCTTTAGTGAGCGTTGTTGTGAATTTGCGATGGTCAATCCACGCCGAGAAGGGTTGTCG
GCTCGTTATGCATGGATGGCTGTGGCAGAGCGTGAAACCGGCTATGCCCCCCTACAGGCGATCAAGAAACTGGATTTGAG
CAATGGTGATCAATGCTTATGGAGTGCAGCGCCGCGTGGATTTGTCAGTGAGCCATTGATGGTGCCACGCCCTGGCAGTG
AAGCTGAGGATGATGGTTGGCTGTTGGTTTTTGTATGGAATGGAGAGCGTCGTGCCAGTCAGTTGTTGATTTTGAATGCA
AACGATTTAAGCGAGCAGGCAGTGCTTGAGATGCCTATAACCATTCCATACGGATTACATGGCAGCTGGGTTGCTGCTGA
TTGA

Upstream 100 bases:

>100_bases
TGTGCTGGAGCAGGCTGGCGGGCAGAAGAGCTGAATGCTTTGGGAGTGGGGATTTCCTCGCTCGCGGCTGTAAGCCCTTT
TGCGTAAGGATTGGTCAATC

Downstream 100 bases:

>100_bases
TAGATTGAGTTGAGGGTATTACTTAGCGAACAAAATCCAAGCTTATTTGCAATTTGTTCGCTCTAGACTTAAGGATTTGG
TTGGTTGACTAGATAAAGTC

Product: retinal pigment epithelial membrane protein

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MISLAVSTVSDSPSTVSATSIDRPGGFDRDDWASGYCNVEQELNAVSLRPLRGQIPAELQGSHYRNGPGRFERDGQWVHH
PFDGDGMITAVRFSDSKATLSNRFVRTKYWQEEEKAGRFLYRGVFGTQKRGGAKANAFDVRFKNIANTQVVQLGDQLLAL
WEAAGPHALDPVSLETHGLSSLNGVLKPGEAFSAHPRFDPGHHGEPRMVTFGVNTGPTSKVRLMEFATQGPDAGRLISDR
TDSFKGFAFLHDFAITPNWTLFLQNAINFNPLPFVLGQKGAAQCLTSKPGGQAKFWLIPRDCGTFAGQPPRIFDAPDGFV
FHHLNAWEEGDELVLESIYYNDYPSIDPGEDFLSMDFDFFPEGRLEQCRINLLGKGIETKRFSERCCEFAMVNPRREGLS
ARYAWMAVAERETGYAPLQAIKKLDLSNGDQCLWSAAPRGFVSEPLMVPRPGSEAEDDGWLLVFVWNGERRASQLLILNA
NDLSEQAVLEMPITIPYGLHGSWVAAD

Sequences:

>Translated_507_residues
MISLAVSTVSDSPSTVSATSIDRPGGFDRDDWASGYCNVEQELNAVSLRPLRGQIPAELQGSHYRNGPGRFERDGQWVHH
PFDGDGMITAVRFSDSKATLSNRFVRTKYWQEEEKAGRFLYRGVFGTQKRGGAKANAFDVRFKNIANTQVVQLGDQLLAL
WEAAGPHALDPVSLETHGLSSLNGVLKPGEAFSAHPRFDPGHHGEPRMVTFGVNTGPTSKVRLMEFATQGPDAGRLISDR
TDSFKGFAFLHDFAITPNWTLFLQNAINFNPLPFVLGQKGAAQCLTSKPGGQAKFWLIPRDCGTFAGQPPRIFDAPDGFV
FHHLNAWEEGDELVLESIYYNDYPSIDPGEDFLSMDFDFFPEGRLEQCRINLLGKGIETKRFSERCCEFAMVNPRREGLS
ARYAWMAVAERETGYAPLQAIKKLDLSNGDQCLWSAAPRGFVSEPLMVPRPGSEAEDDGWLLVFVWNGERRASQLLILNA
NDLSEQAVLEMPITIPYGLHGSWVAAD
>Mature_507_residues
MISLAVSTVSDSPSTVSATSIDRPGGFDRDDWASGYCNVEQELNAVSLRPLRGQIPAELQGSHYRNGPGRFERDGQWVHH
PFDGDGMITAVRFSDSKATLSNRFVRTKYWQEEEKAGRFLYRGVFGTQKRGGAKANAFDVRFKNIANTQVVQLGDQLLAL
WEAAGPHALDPVSLETHGLSSLNGVLKPGEAFSAHPRFDPGHHGEPRMVTFGVNTGPTSKVRLMEFATQGPDAGRLISDR
TDSFKGFAFLHDFAITPNWTLFLQNAINFNPLPFVLGQKGAAQCLTSKPGGQAKFWLIPRDCGTFAGQPPRIFDAPDGFV
FHHLNAWEEGDELVLESIYYNDYPSIDPGEDFLSMDFDFFPEGRLEQCRINLLGKGIETKRFSERCCEFAMVNPRREGLS
ARYAWMAVAERETGYAPLQAIKKLDLSNGDQCLWSAAPRGFVSEPLMVPRPGSEAEDDGWLLVFVWNGERRASQLLILNA
NDLSEQAVLEMPITIPYGLHGSWVAAD

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 56225; Mature: 56225

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLAVSTVSDSPSTVSATSIDRPGGFDRDDWASGYCNVEQELNAVSLRPLRGQIPAELQ
CEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCCCC
GSHYRNGPGRFERDGQWVHHPFDGDGMITAVRFSDSKATLSNRFVRTKYWQEEEKAGRFL
CCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHCEEEHHHCCCHHHHCCEE
YRGVFGTQKRGGAKANAFDVRFKNIANTQVVQLGDQLLALWEAAGPHALDPVSLETHGLS
EECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SLNGVLKPGEAFSAHPRFDPGHHGEPRMVTFGVNTGPTSKVRLMEFATQGPDAGRLISDR
HHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEHHHCCCCCCCEECCC
TDSFKGFAFLHDFAITPNWTLFLQNAINFNPLPFVLGQKGAAQCLTSKPGGQAKFWLIPR
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCEEECCCCHHHHHCCCCCCCEEEEEEEC
DCGTFAGQPPRIFDAPDGFVFHHLNAWEEGDELVLESIYYNDYPSIDPGEDFLSMDFDFF
CCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHEEHHHHCCCCCCCCCCCHHEECCCCCC
PEGRLEQCRINLLGKGIETKRFSERCCEFAMVNPRREGLSARYAWMAVAERETGYAPLQA
CCCCHHHHEEEECCCCCCHHHHHHHHHHHEECCCHHHCCCCEEEEEEEECCCCCCHHHHH
IKKLDLSNGDQCLWSAAPRGFVSEPLMVPRPGSEAEDDGWLLVFVWNGERRASQLLILNA
HHHCCCCCCCEEEEECCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEC
NDLSEQAVLEMPITIPYGLHGSWVAAD
CCCCCCEEEEECEEEECCCCCCEEECC
>Mature Secondary Structure
MISLAVSTVSDSPSTVSATSIDRPGGFDRDDWASGYCNVEQELNAVSLRPLRGQIPAELQ
CEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCCCC
GSHYRNGPGRFERDGQWVHHPFDGDGMITAVRFSDSKATLSNRFVRTKYWQEEEKAGRFL
CCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHCEEEHHHCCCHHHHCCEE
YRGVFGTQKRGGAKANAFDVRFKNIANTQVVQLGDQLLALWEAAGPHALDPVSLETHGLS
EECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SLNGVLKPGEAFSAHPRFDPGHHGEPRMVTFGVNTGPTSKVRLMEFATQGPDAGRLISDR
HHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEHHHCCCCCCCEECCC
TDSFKGFAFLHDFAITPNWTLFLQNAINFNPLPFVLGQKGAAQCLTSKPGGQAKFWLIPR
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCEEECCCCHHHHHCCCCCCCEEEEEEEC
DCGTFAGQPPRIFDAPDGFVFHHLNAWEEGDELVLESIYYNDYPSIDPGEDFLSMDFDFF
CCCCCCCCCCEEEECCCCEEEEECCCCCCCCHHEEHHHHCCCCCCCCCCCHHEECCCCCC
PEGRLEQCRINLLGKGIETKRFSERCCEFAMVNPRREGLSARYAWMAVAERETGYAPLQA
CCCCHHHHEEEECCCCCCHHHHHHHHHHHEECCCHHHCCCCEEEEEEEECCCCCCHHHHH
IKKLDLSNGDQCLWSAAPRGFVSEPLMVPRPGSEAEDDGWLLVFVWNGERRASQLLILNA
HHHCCCCCCCEEEEECCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEC
NDLSEQAVLEMPITIPYGLHGSWVAAD
CCCCCCEEEEECEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]