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The map label for this gene is cobS

Identifier: 124024178

GI number: 124024178

Start: 2186718

End: 2187500

Strand: Reverse

Name: cobS

Synonym: P9303_24891

Alternate gene names: 124024178

Gene position: 2187500-2186718 (Counterclockwise)

Preceding gene: 124024181

Following gene: 124024176

Centisome position: 81.54

GC content: 56.58

Gene sequence:

>783_bases
TTGCGCGCTCCCCTTTGGCTCCGGGATTTGGCTGGAGCATGGATCTTCTACTCCGTATTGCCTGCTTGGCCAGGGCTGAA
GCCACGTTTTGAACGGATTGCTCGCTTTGCTCCTTGGATCGGATTGGTGCTGGGAGGACTGCAGTCTTTCCTTTGGCTTG
TCTTGATCCGTGCCGATTGGCCAACCTCGGCAGTGGCACTTTTGGTCATCGGGCTGGGTGCCTGGCTTTCGGGTGGTCTT
CACCTTGATGGGTTGATGGACACGGCCGATGGTCTTGCGGCTGGTCGTGAGCGCTGTCTGCAGGCTATGGACGACAGCTG
TGTTGGAGCTAGTGGAGTCCAGGCTCTGCTTGTGGTGGTGTTACTTCAGATTGCAGCCCTACTTCGGCTTGGTTCTCTAG
CCCCTTTGGCTTTGCTGATTGCTGCCTTTTGGGGGCGTTGTGCGCCGCTTTGGGCCATGGCGCGCTTCTTTTACTTGCGT
GAGGGGCAGGCTGGCAGCGCATCCTTTCATCGTCGTTATCGCCAGGGATGGCAGGAAGCATTGCCAGCATTCTTAGTGCT
GCTTTTGGCATTAACGGTTGTCCCTTTACTCATGATCGTGGGGTGGCCCAGTCAGATGGTTTTGATGGCAGGAATTGGTG
TGGGTGTCTTGCCGGCGTTTTTAGTGCCTGAACTGCTTGGACGTCGTCTTGGTGGGCATAGCGGGGATTCTTATGGCGCG
TCAGTCGTGCTGGTGGAAACAATCACGCTTCTGTTGCTGGCGGTGTTACTGACGGCAGGCTGA

Upstream 100 bases:

>100_bases
TCAAACACGGCTAGTGCTACGGCGATACTCCAGCGCCACAGGGCACCAGGCCGATCATGACCCTACGTTGGGCTTTGCCT
TTGACTGGAGGTTGGAGATC

Downstream 100 bases:

>100_bases
GCAGAAAGGCATTGCCTTCGCTGGGAAGGGCTGGATCAAGGTTCTCTGGCGAGGTGATCAGTTGCAGCGAACCTCCCAAA
TCTTCAGCCAGTTGGCGTCC

Product: cobalamin-5-phosphate synthase CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MRAPLWLRDLAGAWIFYSVLPAWPGLKPRFERIARFAPWIGLVLGGLQSFLWLVLIRADWPTSAVALLVIGLGAWLSGGL
HLDGLMDTADGLAAGRERCLQAMDDSCVGASGVQALLVVVLLQIAALLRLGSLAPLALLIAAFWGRCAPLWAMARFFYLR
EGQAGSASFHRRYRQGWQEALPAFLVLLLALTVVPLLMIVGWPSQMVLMAGIGVGVLPAFLVPELLGRRLGGHSGDSYGA
SVVLVETITLLLLAVLLTAG

Sequences:

>Translated_260_residues
MRAPLWLRDLAGAWIFYSVLPAWPGLKPRFERIARFAPWIGLVLGGLQSFLWLVLIRADWPTSAVALLVIGLGAWLSGGL
HLDGLMDTADGLAAGRERCLQAMDDSCVGASGVQALLVVVLLQIAALLRLGSLAPLALLIAAFWGRCAPLWAMARFFYLR
EGQAGSASFHRRYRQGWQEALPAFLVLLLALTVVPLLMIVGWPSQMVLMAGIGVGVLPAFLVPELLGRRLGGHSGDSYGA
SVVLVETITLLLLAVLLTAG
>Mature_260_residues
MRAPLWLRDLAGAWIFYSVLPAWPGLKPRFERIARFAPWIGLVLGGLQSFLWLVLIRADWPTSAVALLVIGLGAWLSGGL
HLDGLMDTADGLAAGRERCLQAMDDSCVGASGVQALLVVVLLQIAALLRLGSLAPLALLIAAFWGRCAPLWAMARFFYLR
EGQAGSASFHRRYRQGWQEALPAFLVLLLALTVVPLLMIVGWPSQMVLMAGIGVGVLPAFLVPELLGRRLGGHSGDSYGA
SVVLVETITLLLLAVLLTAG

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=234, Percent_Identity=34.6153846153846, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_PROM3 (A2CCL0)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001018485.1
- STRING:   A2CCL0
- GeneID:   4776974
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_24891
- eggNOG:   COG0368
- HOGENOM:   HBG729392
- OMA:   GWGRWGQ
- ProtClustDB:   CLSK647355
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 27901; Mature: 27901

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x16316118)-; HASH(0x179bad6c)-; HASH(0x1769f47c)-; HASH(0x17b2602c)-; HASH(0x17682a88)-; HASH(0x16cbde9c)-; HASH(0x179bad78)-; HASH(0x178a582c)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAPLWLRDLAGAWIFYSVLPAWPGLKPRFERIARFAPWIGLVLGGLQSFLWLVLIRADW
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTSAVALLVIGLGAWLSGGLHLDGLMDTADGLAAGRERCLQAMDDSCVGASGVQALLVVV
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LLQIAALLRLGSLAPLALLIAAFWGRCAPLWAMARFFYLREGQAGSASFHRRYRQGWQEA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LPAFLVLLLALTVVPLLMIVGWPSQMVLMAGIGVGVLPAFLVPELLGRRLGGHSGDSYGA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCH
SVVLVETITLLLLAVLLTAG
HHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MRAPLWLRDLAGAWIFYSVLPAWPGLKPRFERIARFAPWIGLVLGGLQSFLWLVLIRADW
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTSAVALLVIGLGAWLSGGLHLDGLMDTADGLAAGRERCLQAMDDSCVGASGVQALLVVV
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LLQIAALLRLGSLAPLALLIAAFWGRCAPLWAMARFFYLREGQAGSASFHRRYRQGWQEA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LPAFLVLLLALTVVPLLMIVGWPSQMVLMAGIGVGVLPAFLVPELLGRRLGGHSGDSYGA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCH
SVVLVETITLLLLAVLLTAG
HHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA