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The map label for this gene is recF [H]

Identifier: 124023971

GI number: 124023971

Start: 2013493

End: 2014551

Strand: Direct

Name: recF [H]

Synonym: P9303_22781

Alternate gene names: 124023971

Gene position: 2013493-2014551 (Clockwise)

Preceding gene: 124023968

Following gene: 124023972

Centisome position: 75.06

GC content: 56.85

Gene sequence:

>1059_bases
TTGACGGAAAATCGGCTGCTGGTCATTGGCCCCAACGGCATCGGTAAATCCAACCTTCTCGAAGCAGTAGAGCTGCTCGG
CAGTCTGCGCTCGCATCGCGCCAGCAGCGACCAAGACTTGATCCACTGGGAAGAACAGCGGGCCCTACTTCGCGCCATCG
CTGATGACACTGAGAAGCTCGAACTGGAATTGCGGCGGCAAGGAGGTCGACAAGCCCGCCGCAACGGCAAAACGCTCACC
CGCCAATTGGATCTAATCGGTCCACTGCGTTGCGTGGGGTTTAGCGCTCTCGATCTCAATCTCGTTCGAGGCGAGCCTGC
CCTGCGGCGCCAATGGCTTGATCGCGTCGTACAACAACTTGAACCGATCTATTCAGATCTGATCAGTCGCTTCAACAAGC
TGCTGCGTCAACGAAGCCAGCTGTGGCGTCAGTGGCGCCATATCCCGATTCAGGAGCGTGATTCCCTCTTGGATGCCTTC
GATGTACAGATGGCTTTAGTCAGCACCCGCATTCATCGACGTCGCAGTCGAGCTCTCGCCCGCCTTGAACCACTTGCAGC
CCGATGGCAGGAAACCCTCAGCAAACAGAAGGAACGGTTGCGACTCGACTATCAGCCAGGCAGCCAACTGGAAGGTGAGG
AAGCGGAAGAACCTTGGCGATTGGCGATCGAAACACAGCTCCTAGACCAACGCAGCGAAGAGGAACGATTGGGAAGTTGC
AGGATCGGTCCTCACCGCGACGAGGTGAGATTGCTACTCAACGATTCAGAAGCACGCCGCTTTGGCTCAGCAGGGCAACA
GCGAACCGTGGTGCTGGCACTCAAGCTTGCCGAACTGGAACTCGTGGGCGAACTCTGCGGTGAACCCCCTCTGCTGTTAT
TGGATGACGTTCTTGCGGAACTGGATCCCGGCAGACAGTTGCTGCTGCTCGAGGCTGTCGGAGAGAAGCATCAGTGCCTC
GTTAGCGCGACCCATTTGGAGGGTTTCCAGGATGAATGGCAACAACGATCACAGATCATCGAAGCAGGATCTTTGAATCG
ATCGGAAGAGGTCCGCTAA

Upstream 100 bases:

>100_bases
CTCAATCAAGGTACCTTAACCTCATGCCGCTCCAAGACCGATTCGGCTTCACCAGCTCGAACTGCAGGGGTACCGCAATT
ACAGCAGGCTTCAGCTGGAG

Downstream 100 bases:

>100_bases
GGTGGCGAGCTGATTTTTATTGCCTTGATGGAGAGAACCCTGTCTTGCTTGCATCCCAATCCAGGATGGGAAGACACCGA
TTGCCAAGACAACGCAAGCT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTENRLLVIGPNGIGKSNLLEAVELLGSLRSHRASSDQDLIHWEEQRALLRAIADDTEKLELELRRQGGRQARRNGKTLT
RQLDLIGPLRCVGFSALDLNLVRGEPALRRQWLDRVVQQLEPIYSDLISRFNKLLRQRSQLWRQWRHIPIQERDSLLDAF
DVQMALVSTRIHRRRSRALARLEPLAARWQETLSKQKERLRLDYQPGSQLEGEEAEEPWRLAIETQLLDQRSEEERLGSC
RIGPHRDEVRLLLNDSEARRFGSAGQQRTVVLALKLAELELVGELCGEPPLLLLDDVLAELDPGRQLLLLEAVGEKHQCL
VSATHLEGFQDEWQQRSQIIEAGSLNRSEEVR

Sequences:

>Translated_352_residues
MTENRLLVIGPNGIGKSNLLEAVELLGSLRSHRASSDQDLIHWEEQRALLRAIADDTEKLELELRRQGGRQARRNGKTLT
RQLDLIGPLRCVGFSALDLNLVRGEPALRRQWLDRVVQQLEPIYSDLISRFNKLLRQRSQLWRQWRHIPIQERDSLLDAF
DVQMALVSTRIHRRRSRALARLEPLAARWQETLSKQKERLRLDYQPGSQLEGEEAEEPWRLAIETQLLDQRSEEERLGSC
RIGPHRDEVRLLLNDSEARRFGSAGQQRTVVLALKLAELELVGELCGEPPLLLLDDVLAELDPGRQLLLLEAVGEKHQCL
VSATHLEGFQDEWQQRSQIIEAGSLNRSEEVR
>Mature_351_residues
TENRLLVIGPNGIGKSNLLEAVELLGSLRSHRASSDQDLIHWEEQRALLRAIADDTEKLELELRRQGGRQARRNGKTLTR
QLDLIGPLRCVGFSALDLNLVRGEPALRRQWLDRVVQQLEPIYSDLISRFNKLLRQRSQLWRQWRHIPIQERDSLLDAFD
VQMALVSTRIHRRRSRALARLEPLAARWQETLSKQKERLRLDYQPGSQLEGEEAEEPWRLAIETQLLDQRSEEERLGSCR
IGPHRDEVRLLLNDSEARRFGSAGQQRTVVLALKLAELELVGELCGEPPLLLLDDVLAELDPGRQLLLLEAVGEKHQCLV
SATHLEGFQDEWQQRSQIIEAGSLNRSEEVR

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=351, Percent_Identity=25.9259259259259, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40505; Mature: 40374

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENRLLVIGPNGIGKSNLLEAVELLGSLRSHRASSDQDLIHWEEQRALLRAIADDTEKL
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHH
ELELRRQGGRQARRNGKTLTRQLDLIGPLRCVGFSALDLNLVRGEPALRRQWLDRVVQQL
HHHHHHCCCHHHHHCCHHHHHHHHHHCHHHHHCCHHHEEEEECCCHHHHHHHHHHHHHHH
EPIYSDLISRFNKLLRQRSQLWRQWRHIPIQERDSLLDAFDVQMALVSTRIHRRRSRALA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLEPLAARWQETLSKQKERLRLDYQPGSQLEGEEAEEPWRLAIETQLLDQRSEEERLGSC
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCC
RIGPHRDEVRLLLNDSEARRFGSAGQQRTVVLALKLAELELVGELCGEPPLLLLDDVLAE
CCCCCCCHHEEEECCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHH
LDPGRQLLLLEAVGEKHQCLVSATHLEGFQDEWQQRSQIIEAGSLNRSEEVR
CCCCCEEEEEHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TENRLLVIGPNGIGKSNLLEAVELLGSLRSHRASSDQDLIHWEEQRALLRAIADDTEKL
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHH
ELELRRQGGRQARRNGKTLTRQLDLIGPLRCVGFSALDLNLVRGEPALRRQWLDRVVQQL
HHHHHHCCCHHHHHCCHHHHHHHHHHCHHHHHCCHHHEEEEECCCHHHHHHHHHHHHHHH
EPIYSDLISRFNKLLRQRSQLWRQWRHIPIQERDSLLDAFDVQMALVSTRIHRRRSRALA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLEPLAARWQETLSKQKERLRLDYQPGSQLEGEEAEEPWRLAIETQLLDQRSEEERLGSC
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCC
RIGPHRDEVRLLLNDSEARRFGSAGQQRTVVLALKLAELELVGELCGEPPLLLLDDVLAE
CCCCCCCHHEEEECCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHH
LDPGRQLLLLEAVGEKHQCLVSATHLEGFQDEWQQRSQIIEAGSLNRSEEVR
CCCCCEEEEEHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]