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The map label for this gene is lipB [H]

Identifier: 124023833

GI number: 124023833

Start: 1903809

End: 1904570

Strand: Reverse

Name: lipB [H]

Synonym: P9303_21401

Alternate gene names: 124023833

Gene position: 1904570-1903809 (Counterclockwise)

Preceding gene: 124023839

Following gene: 124023832

Centisome position: 71.0

GC content: 56.04

Gene sequence:

>762_bases
ATGGATCAGCAGCTTCATGGCATCCCTCCGGTGCCGATTGATATCGGCAAGTTAGGTACTACCTCTACATCGGCACAATC
CCGATCGACTTTTCTGTTTGAGTGCAGTGAGCCAGTGCCCTTCGAGATGGCTTGGCTTTGGCAGCAGCAGTGGCAGCAAC
ATTTGTTTGCCAAACCCCTTGCTCCGCAGGCGGTTTGGTTGTTGCAGCATCAGGCTTGCTACACCCTCGGTCGTGGTGCA
AGCGAGGCCAATCTGCTTTTTGATGCCAACAATCCGCCTGCTGCGTTGCATCGCATTGATCGGGGTGGTGAGGTGACCCA
CCATTTGCCTGGGCAGTTGGTGGTCTATCCCGTGTTGGATCTTCGGCGTTATCAAACCGATCTGCATTGGTATCTGCGTC
AGCTTGAGCAGGTATTGATTGATGTTTTGGCTGTTTTGGGATTAGCAGGTGAGCGCTTGCAGGGACTCACCGGGCTATGG
CTGGAAGGTCGCAAGGTGGCTGCGATTGGCGTGGGGTGTCGTCGCTGGATTACTCAACATGGATTGGCTCTCAACGTGGA
TTGTGATCTTGAGAGCTTTTCCGCCGTTGTCCCCTGCGGCCTGGTGGGTCACCCGGTGGGTCGGCTGAATCAATGGATCC
CTGGGCTCACGGTGGCGGAGGTGCAGCCTCTGATGCGTCAGAGCCTGAGTGACCGTTTCGGTTTGGTTTGGCAGATCCCA
GAGTCTCTGGCCAAGCCAGGGGCATTTGCGTCCGATGCTTAG

Upstream 100 bases:

>100_bases
CATCAGCTTCCTTCACCATGTCTTCAAAGTGTTGAATCGCCCGCTCCAGTTTTGATTGTGTGTATTCGCGTAGGGCAGGT
GTTAGTTCAAGATTGCGTCC

Downstream 100 bases:

>100_bases
CGCTCTTAGCCCTGTTTGCATTGAAGATGTCAGATGAGATAGCAGCAGGTAGCAGGTTGAAAGACGACAACCATGAGGGT
TCTCTAGCCAAGGCGGTTTG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MDQQLHGIPPVPIDIGKLGTTSTSAQSRSTFLFECSEPVPFEMAWLWQQQWQQHLFAKPLAPQAVWLLQHQACYTLGRGA
SEANLLFDANNPPAALHRIDRGGEVTHHLPGQLVVYPVLDLRRYQTDLHWYLRQLEQVLIDVLAVLGLAGERLQGLTGLW
LEGRKVAAIGVGCRRWITQHGLALNVDCDLESFSAVVPCGLVGHPVGRLNQWIPGLTVAEVQPLMRQSLSDRFGLVWQIP
ESLAKPGAFASDA

Sequences:

>Translated_253_residues
MDQQLHGIPPVPIDIGKLGTTSTSAQSRSTFLFECSEPVPFEMAWLWQQQWQQHLFAKPLAPQAVWLLQHQACYTLGRGA
SEANLLFDANNPPAALHRIDRGGEVTHHLPGQLVVYPVLDLRRYQTDLHWYLRQLEQVLIDVLAVLGLAGERLQGLTGLW
LEGRKVAAIGVGCRRWITQHGLALNVDCDLESFSAVVPCGLVGHPVGRLNQWIPGLTVAEVQPLMRQSLSDRFGLVWQIP
ESLAKPGAFASDA
>Mature_253_residues
MDQQLHGIPPVPIDIGKLGTTSTSAQSRSTFLFECSEPVPFEMAWLWQQQWQQHLFAKPLAPQAVWLLQHQACYTLGRGA
SEANLLFDANNPPAALHRIDRGGEVTHHLPGQLVVYPVLDLRRYQTDLHWYLRQLEQVLIDVLAVLGLAGERLQGLTGLW
LEGRKVAAIGVGCRRWITQHGLALNVDCDLESFSAVVPCGLVGHPVGRLNQWIPGLTVAEVQPLMRQSLSDRFGLVWQIP
ESLAKPGAFASDA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=197, Percent_Identity=39.0862944162437, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI87081767, Length=165, Percent_Identity=32.1212121212121, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323268, Length=124, Percent_Identity=35.4838709677419, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21355483, Length=158, Percent_Identity=34.8101265822785, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 28029; Mature: 28029

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQQLHGIPPVPIDIGKLGTTSTSAQSRSTFLFECSEPVPFEMAWLWQQQWQQHLFAKPL
CCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
APQAVWLLQHQACYTLGRGASEANLLFDANNPPAALHRIDRGGEVTHHLPGQLVVYPVLD
CHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCCCCEEECCCCCEEEEEHHH
LRRYQTDLHWYLRQLEQVLIDVLAVLGLAGERLQGLTGLWLEGRKVAAIGVGCRRWITQH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECCCEEEEECHHHHHHHHHC
GLALNVDCDLESFSAVVPCGLVGHPVGRLNQWIPGLTVAEVQPLMRQSLSDRFGLVWQIP
CCEEEEECCHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEECC
ESLAKPGAFASDA
HHHCCCCCCCCCC
>Mature Secondary Structure
MDQQLHGIPPVPIDIGKLGTTSTSAQSRSTFLFECSEPVPFEMAWLWQQQWQQHLFAKPL
CCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
APQAVWLLQHQACYTLGRGASEANLLFDANNPPAALHRIDRGGEVTHHLPGQLVVYPVLD
CHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCCCCEEECCCCCEEEEEHHH
LRRYQTDLHWYLRQLEQVLIDVLAVLGLAGERLQGLTGLWLEGRKVAAIGVGCRRWITQH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECCCEEEEECHHHHHHHHHC
GLALNVDCDLESFSAVVPCGLVGHPVGRLNQWIPGLTVAEVQPLMRQSLSDRFGLVWQIP
CCEEEEECCHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEECC
ESLAKPGAFASDA
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]