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The map label for this gene is queA

Identifier: 124023814

GI number: 124023814

Start: 1881235

End: 1882350

Strand: Reverse

Name: queA

Synonym: P9303_21211

Alternate gene names: 124023814

Gene position: 1882350-1881235 (Counterclockwise)

Preceding gene: 124023815

Following gene: 124023810

Centisome position: 70.17

GC content: 57.26

Gene sequence:

>1116_bases
ATGGTTCTAGATCCAAGAGATTCTCTGCTCAGTTCTTATGACTATCCGCTTGATCCTGAGCGCATAGCTCAGCTGCCTGT
GGAGCCAAGGCATGCGGCGCGGCTTTTGATAGTTCAGCCATTGGGGATGAAGCCTCCCATCGCTCGACATGCCGAGGTTT
GGGATTGGCAGGAGGAATTGCGCGCTGGTGATCTGCTTGTCATCAATGACACTCGCGTTCTTAAGGCTCGGCTGCGGGTG
CGGCGATCAGGTGGTGGCTTGGCTGAGTTGTTGGTGCTTGAGCCGCGTGGTGAGGGTCGCTGGCTGTGTTTGGGCCGGCC
TGCTAAACGTTTGCGCCCAGGTGATCAGCTTTGGCTTGAGGCTTTGGGCGAGGAGCCCATTGCTTTGCAGGTGATTAGCA
AAGACAGCGCTAGTGGCGGACGGGTTGTGCAGTTCCCGAGCCATTACGCCAATGCCGAGCAGCTTGAGTTGCTGCTTGAG
CGCTACGGCGAAGTGCCATTACCGCCTTATATCAAATGTCATGATCCAAGTGATAGCGAGCGTTATCAGACCCGTTATGC
ATCCCGCCCCGGCGCCGTTGCGGCGCCAACGGCGGGGCTTCATCTCAGTGATGAGTTATTGGAGGCTCTCAAGCAGCGGG
GAGTGATGCTGACCAGTGTCACCTTGCATGTGGGCTTAGGCACGTTTCGACCTGTTGAAACTGAGGATCTGAGGCATTTG
CAGCTTCACAGCGAGTGGGTGGAGGTGCGCGACGAAGTTGTTGCGGCGGTGATGGCTTGTCGAGCACGAGCAGGCCGGGT
GATTGCTGTGGGAACCACCAGCGTCAGGGCCCTTGAAGGTGCTGCTTTGGCAGGTGGCGGATTCCTGCAGTCGTTTTGTG
GACCAGTGGATTTGGTGATTCAGCCGGGTTATCGCTTTGCCGTGGTGGATGGACTGCTCACCAACTTCCATTTGCCGAAA
AGCTCTCTATTGCTGTTGGTGAGTGCCATGGTTGGTCGTGAACGGCTGCTGGCGCTCTATGCCGAGGCCATTGAGCACCA
CTATCGCTTTTTCTCTTATGGCGATGCGATGTGGATTGATCCAGGGGCGGTGTTGCCAGCGGCCCGCCCCTGCTGA

Upstream 100 bases:

>100_bases
ATAGATAGGGATAACCAGCGGACTAAAAACTTGCTGGTTTGTGAACCCAGAACTTTTTGAACGGTTTTTTGAACGTCACT
CATAAAGCAATACCTCACGA

Downstream 100 bases:

>100_bases
TCATTTCCAGGTGGAGGTGATTTAGGCAGCTACAGGGTCCTGAATGATGCCTGTGGGCACACTCTCAAACATCACTGAGG
AGAGATAACGCTCGGCCATG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MVLDPRDSLLSSYDYPLDPERIAQLPVEPRHAARLLIVQPLGMKPPIARHAEVWDWQEELRAGDLLVINDTRVLKARLRV
RRSGGGLAELLVLEPRGEGRWLCLGRPAKRLRPGDQLWLEALGEEPIALQVISKDSASGGRVVQFPSHYANAEQLELLLE
RYGEVPLPPYIKCHDPSDSERYQTRYASRPGAVAAPTAGLHLSDELLEALKQRGVMLTSVTLHVGLGTFRPVETEDLRHL
QLHSEWVEVRDEVVAAVMACRARAGRVIAVGTTSVRALEGAALAGGGFLQSFCGPVDLVIQPGYRFAVVDGLLTNFHLPK
SSLLLLVSAMVGRERLLALYAEAIEHHYRFFSYGDAMWIDPGAVLPAARPC

Sequences:

>Translated_371_residues
MVLDPRDSLLSSYDYPLDPERIAQLPVEPRHAARLLIVQPLGMKPPIARHAEVWDWQEELRAGDLLVINDTRVLKARLRV
RRSGGGLAELLVLEPRGEGRWLCLGRPAKRLRPGDQLWLEALGEEPIALQVISKDSASGGRVVQFPSHYANAEQLELLLE
RYGEVPLPPYIKCHDPSDSERYQTRYASRPGAVAAPTAGLHLSDELLEALKQRGVMLTSVTLHVGLGTFRPVETEDLRHL
QLHSEWVEVRDEVVAAVMACRARAGRVIAVGTTSVRALEGAALAGGGFLQSFCGPVDLVIQPGYRFAVVDGLLTNFHLPK
SSLLLLVSAMVGRERLLALYAEAIEHHYRFFSYGDAMWIDPGAVLPAARPC
>Mature_371_residues
MVLDPRDSLLSSYDYPLDPERIAQLPVEPRHAARLLIVQPLGMKPPIARHAEVWDWQEELRAGDLLVINDTRVLKARLRV
RRSGGGLAELLVLEPRGEGRWLCLGRPAKRLRPGDQLWLEALGEEPIALQVISKDSASGGRVVQFPSHYANAEQLELLLE
RYGEVPLPPYIKCHDPSDSERYQTRYASRPGAVAAPTAGLHLSDELLEALKQRGVMLTSVTLHVGLGTFRPVETEDLRHL
QLHSEWVEVRDEVVAAVMACRARAGRVIAVGTTSVRALEGAALAGGGFLQSFCGPVDLVIQPGYRFAVVDGLLTNFHLPK
SSLLLLVSAMVGRERLLALYAEAIEHHYRFFSYGDAMWIDPGAVLPAARPC

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=360, Percent_Identity=42.2222222222222, Blast_Score=258, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_PROM3 (A2CBJ6)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001018121.1
- ProteinModelPortal:   A2CBJ6
- SMR:   A2CBJ6
- STRING:   A2CBJ6
- GeneID:   4777086
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_21211
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   CYSHEIN
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40967; Mature: 40967

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLDPRDSLLSSYDYPLDPERIAQLPVEPRHAARLLIVQPLGMKPPIARHAEVWDWQEEL
CCCCCHHHHHHCCCCCCCHHHHHCCCCCCCHHCEEEEEECCCCCCCCCCCCHHCCHHHHH
RAGDLLVINDTRVLKARLRVRRSGGGLAELLVLEPRGEGRWLCLGRPAKRLRPGDQLWLE
CCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCCHHHCCCCHHHHHH
ALGEEPIALQVISKDSASGGRVVQFPSHYANAEQLELLLERYGEVPLPPYIKCHDPSDSE
HHCCCCEEEEEEECCCCCCCEEEECCHHHCCHHHHHHHHHHHCCCCCCCEEEECCCCCCH
RYQTRYASRPGAVAAPTAGLHLSDELLEALKQRGVMLTSVTLHVGLGTFRPVETEDLRHL
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEEEEECCCCCCCCCHHHHHHH
QLHSEWVEVRDEVVAAVMACRARAGRVIAVGTTSVRALEGAALAGGGFLQSFCGPVDLVI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCEECCCHHHHHHCCCEEEEE
QPGYRFAVVDGLLTNFHLPKSSLLLLVSAMVGRERLLALYAEAIEHHYRFFSYGDAMWID
CCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEC
PGAVLPAARPC
CCCCCCCCCCC
>Mature Secondary Structure
MVLDPRDSLLSSYDYPLDPERIAQLPVEPRHAARLLIVQPLGMKPPIARHAEVWDWQEEL
CCCCCHHHHHHCCCCCCCHHHHHCCCCCCCHHCEEEEEECCCCCCCCCCCCHHCCHHHHH
RAGDLLVINDTRVLKARLRVRRSGGGLAELLVLEPRGEGRWLCLGRPAKRLRPGDQLWLE
CCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCCHHHCCCCHHHHHH
ALGEEPIALQVISKDSASGGRVVQFPSHYANAEQLELLLERYGEVPLPPYIKCHDPSDSE
HHCCCCEEEEEEECCCCCCCEEEECCHHHCCHHHHHHHHHHHCCCCCCCEEEECCCCCCH
RYQTRYASRPGAVAAPTAGLHLSDELLEALKQRGVMLTSVTLHVGLGTFRPVETEDLRHL
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEEEEEECCCCCCCCCHHHHHHH
QLHSEWVEVRDEVVAAVMACRARAGRVIAVGTTSVRALEGAALAGGGFLQSFCGPVDLVI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCEECCCHHHHHHCCCEEEEE
QPGYRFAVVDGLLTNFHLPKSSLLLLVSAMVGRERLLALYAEAIEHHYRFFSYGDAMWID
CCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEC
PGAVLPAARPC
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA