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The map label for this gene is cbiB [H]

Identifier: 124023706

GI number: 124023706

Start: 1765059

End: 1765967

Strand: Direct

Name: cbiB [H]

Synonym: P9303_20061

Alternate gene names: 124023706

Gene position: 1765059-1765967 (Clockwise)

Preceding gene: 124023705

Following gene: 124023707

Centisome position: 65.79

GC content: 61.72

Gene sequence:

>909_bases
ATGGGATCTGTGATCAAACATCTGCGTCATCTTGCCGAAGCGATCGCCGGAAATCGCCCCGTGCTGCTGCGTGTTAGCGG
CGTTCTGATCACCGCTGTGCTGATCAGCGGCAGCGGTGCAATGGGTTGGGCAATCGAACGCCTCGCCCTGCAGGAAGGCA
TCGCCCCAGCCTGGCTCGGGATCCCCATGCTGGTGATCGCCCTGGCCAGTGCCCTCGCCGCCGGCAGCCTCCGCCACAGC
ATCTTGAAGGTGTTGAAGGCGCTGCCCACAGACGCGGGAGGAGACCTGTCTATCGCCCGTTTGCAGCTCAGCTGGATCGT
GGGACGCGATGTAACCCAGTTGAATGAGCAGGAGATTCTGCGGGCTACCGCTGAAAGCGCAGCCGAAAACGCCGTGGATG
GCGTGTTTGCACCGCTGTTTTGGATGCTGATCGGTGCTGGCCTCTGGCATGTCTCCAATGCATGGCCAGGCCCTCTAGCC
CTGGCCTGGAGCTTCAAGGCCTCCAGCACCCTTGATTCGATGCTGGGCTATCGGCAGGGAACGCTGCAATGGCTGGGCAC
TGCCGGAGCACGACTCGATGATCTCCTCACCTGGCTCCCCTGCCGCCTGGTGATGCTCACCCTTCCTCTAATCAGCAAGC
CTTGGCGCCAGTTGCCTGCTCTGGTGATGGCAGCGGAACAGGACGGAGCCTTGGATCACTCCCCCAACGCCGGACGGTCC
GAAGCGATCTATGCCCACTGTGCCGGGGTGCGAATGGGCGGACGCAACCGATACGGCAACCGATGGGTGGACAAACCCTT
GCTGGCAATCGATCAACCGCCGGCAGATCCAGATGCTCTGCAGCGCATCCTGGCACTCACCAGTTGGCTTGAACTGGTCT
GGATCATGATTGCGCTGGCTCTGCTCTAA

Upstream 100 bases:

>100_bases
TGAAAACAGCCTGACCCTGGCCATTCTGCTAGTCATCACTGCAGCAGGTCTCGACCTGCTGATCGGTGATCCGCGCTGGT
GTCCACATCCCGTTGAGTTG

Downstream 100 bases:

>100_bases
CCAAGCCAATGCGAATGATCACCATCACCGCCACAGCTCTGCTGCTGCTGTCGGTGCAGATCCCTGCTCAGGCAAAAACC
ACCAGAACCTTCAGCGGTTC

Product: cobalamin biosynthetic protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MGSVIKHLRHLAEAIAGNRPVLLRVSGVLITAVLISGSGAMGWAIERLALQEGIAPAWLGIPMLVIALASALAAGSLRHS
ILKVLKALPTDAGGDLSIARLQLSWIVGRDVTQLNEQEILRATAESAAENAVDGVFAPLFWMLIGAGLWHVSNAWPGPLA
LAWSFKASSTLDSMLGYRQGTLQWLGTAGARLDDLLTWLPCRLVMLTLPLISKPWRQLPALVMAAEQDGALDHSPNAGRS
EAIYAHCAGVRMGGRNRYGNRWVDKPLLAIDQPPADPDALQRILALTSWLELVWIMIALALL

Sequences:

>Translated_302_residues
MGSVIKHLRHLAEAIAGNRPVLLRVSGVLITAVLISGSGAMGWAIERLALQEGIAPAWLGIPMLVIALASALAAGSLRHS
ILKVLKALPTDAGGDLSIARLQLSWIVGRDVTQLNEQEILRATAESAAENAVDGVFAPLFWMLIGAGLWHVSNAWPGPLA
LAWSFKASSTLDSMLGYRQGTLQWLGTAGARLDDLLTWLPCRLVMLTLPLISKPWRQLPALVMAAEQDGALDHSPNAGRS
EAIYAHCAGVRMGGRNRYGNRWVDKPLLAIDQPPADPDALQRILALTSWLELVWIMIALALL
>Mature_301_residues
GSVIKHLRHLAEAIAGNRPVLLRVSGVLITAVLISGSGAMGWAIERLALQEGIAPAWLGIPMLVIALASALAAGSLRHSI
LKVLKALPTDAGGDLSIARLQLSWIVGRDVTQLNEQEILRATAESAAENAVDGVFAPLFWMLIGAGLWHVSNAWPGPLAL
AWSFKASSTLDSMLGYRQGTLQWLGTAGARLDDLLTWLPCRLVMLTLPLISKPWRQLPALVMAAEQDGALDHSPNAGRSE
AIYAHCAGVRMGGRNRYGNRWVDKPLLAIDQPPADPDALQRILALTSWLELVWIMIALALL

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 32465; Mature: 32333

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSVIKHLRHLAEAIAGNRPVLLRVSGVLITAVLISGSGAMGWAIERLALQEGIAPAWLG
CCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHH
IPMLVIALASALAAGSLRHSILKVLKALPTDAGGDLSIARLQLSWIVGRDVTQLNEQEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHEEEHHHHCCCHHHHHHHHHH
RATAESAAENAVDGVFAPLFWMLIGAGLWHVSNAWPGPLALAWSFKASSTLDSMLGYRQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHCHHH
TLQWLGTAGARLDDLLTWLPCRLVMLTLPLISKPWRQLPALVMAAEQDGALDHSPNAGRS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
EAIYAHCAGVRMGGRNRYGNRWVDKPLLAIDQPPADPDALQRILALTSWLELVWIMIALA
HHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LL
HC
>Mature Secondary Structure 
GSVIKHLRHLAEAIAGNRPVLLRVSGVLITAVLISGSGAMGWAIERLALQEGIAPAWLG
CHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHH
IPMLVIALASALAAGSLRHSILKVLKALPTDAGGDLSIARLQLSWIVGRDVTQLNEQEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHEEEHHHHCCCHHHHHHHHHH
RATAESAAENAVDGVFAPLFWMLIGAGLWHVSNAWPGPLALAWSFKASSTLDSMLGYRQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHCHHH
TLQWLGTAGARLDDLLTWLPCRLVMLTLPLISKPWRQLPALVMAAEQDGALDHSPNAGRS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
EAIYAHCAGVRMGGRNRYGNRWVDKPLLAIDQPPADPDALQRILALTSWLELVWIMIALA
HHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LL
HC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]