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The map label for this gene is miaB

Identifier: 124023692

GI number: 124023692

Start: 1751207

End: 1752649

Strand: Direct

Name: miaB

Synonym: P9303_19921

Alternate gene names: 124023692

Gene position: 1751207-1752649 (Clockwise)

Preceding gene: 124023691

Following gene: 124023693

Centisome position: 65.28

GC content: 56.2

Gene sequence:

>1443_bases
TTGTTTCGATCGTTCGCTTCGCCTCTTGCCTTGGTCGCCACCTCCGCCCTCGCTACTAATCCGGCAACAACCGCACAGCA
TCAAGGCAGTTTCTGGATTCAAACCTTCGGTTGCCAGATGAACAAGGCCGATTCAGAGCGCATGGCCGGAATTCTTGAGG
CGATGGGATACCACGAGGCGCCAGCAGAACTAGAAGCGGATCTCGTGCTCTACAACACCTGCACAATTCGCGATAACGCC
GAGCAGAAGGTTTACAGCTACTTAGGACGCCAGGCCCGCCGCAAACGAACACACCCCCATCTGAAGCTGGTCGTTGCCGG
TTGCGTAGCGCAACAGGAAGGAGAAGCGCTACTGCGACGAATCCCTGAACTCGACCTTGTGATGGGGCCCCAACACGCCA
ACCGCCTTGAAGCCCTACTCACACAGGTGGACAATGGTCAGCAGGTTGTCGCCACAGATGACAACCACATTCTCGAGGAC
CTCACCACAGCTCGACGGGACAGCACAATCTGCGCCTGGGTCAATGTGATCTACGGCTGTAACGAGCGTTGTACCTATTG
CGTGGTGCCCTCCGTTCGGGGGAAGGAGCAATCAAGATCGCCAGAGGCGATCCGACTGGAGATCGAGGGCCTGGCCGCCC
GTGGATTTCGAGAGATCACACTGCTTGGCCAAAACATTGATGCTTATGGACGCGATCTGCCCGGGATCACCCCTGAAGGC
CGCCGCCAAAACACTCTCACCGATCTTCTCCATCACATTCATGACGTGGAAGGAATCGAGCGCATCCGCTTTGCGACAAG
CCATCCGCGCTACTTCACGGAACGATTGATCGAAGCTTGCTTTGATCTACCCAAAGTGTGCGAACACTTCCACATCCCCT
TCCAAAGCGGTGATAACGATGTGCTTAAGGCCATGGCTAGGGGTTACACAGTGGAGCGTTATCGACGCATCGTGAACCGA
ATTCGGGAGCTTATGCCTGATGCCGCTATCAGTACTGATGTGATCGTGGCCTTCCCAGGCGAAACCGACGCCCAGTTCCA
AAACACCTTGAACCTCGTCGAGGAAGTGGGTTTCGACCAGGTGAACACCGCGGCTTACTCACCCCGTCCAAATACGCCTG
CAGCCACCTGGAGCAACCAACTCCCCGAAGTGGTGAAAGTGGAGCGACTCAAGCAACTCAATGCGCTGGTGGAACGCGTA
GCCCTGCAGCGCAATAGCCGCTACTCCGGCAAGGTAGAGCAGGTGCTAGCTGAAGGAATCAATCCAAAAAAACCACAACA
GCTGATGGGACGCACCCGCACCAACCGGCTCACATTCTTTGCGGCCGAAGGACCCCAAAGTTGCCGCTATAGCCCAGGCG
ATCTCGTCGACGTTCAAATCAACAGTGTGCGGGCTTTCTCACTGAGCGGAACCCCTTGTGATCAAACCAGAAGTCGGCAC
TGA

Upstream 100 bases:

>100_bases
CAACGGCTGAGCAACTCAACTAAATAAATAGCGCTCACAGACCTCATCTCCAAATCAACACAATCCATCAGGGCTCGACA
CACTGAAGGAGTTGATGAGA

Downstream 100 bases:

>100_bases
TACCTTTGGTCGCTAACCCCCACGGCCTTGCAGGATCTCCATGCCTTCCTCCCGCACCTGTGTAGGCGTGGTGTTCGGCG
GCGCGTCCGAGGAACATGCC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MFRSFASPLALVATSALATNPATTAQHQGSFWIQTFGCQMNKADSERMAGILEAMGYHEAPAELEADLVLYNTCTIRDNA
EQKVYSYLGRQARRKRTHPHLKLVVAGCVAQQEGEALLRRIPELDLVMGPQHANRLEALLTQVDNGQQVVATDDNHILED
LTTARRDSTICAWVNVIYGCNERCTYCVVPSVRGKEQSRSPEAIRLEIEGLAARGFREITLLGQNIDAYGRDLPGITPEG
RRQNTLTDLLHHIHDVEGIERIRFATSHPRYFTERLIEACFDLPKVCEHFHIPFQSGDNDVLKAMARGYTVERYRRIVNR
IRELMPDAAISTDVIVAFPGETDAQFQNTLNLVEEVGFDQVNTAAYSPRPNTPAATWSNQLPEVVKVERLKQLNALVERV
ALQRNSRYSGKVEQVLAEGINPKKPQQLMGRTRTNRLTFFAAEGPQSCRYSPGDLVDVQINSVRAFSLSGTPCDQTRSRH

Sequences:

>Translated_480_residues
MFRSFASPLALVATSALATNPATTAQHQGSFWIQTFGCQMNKADSERMAGILEAMGYHEAPAELEADLVLYNTCTIRDNA
EQKVYSYLGRQARRKRTHPHLKLVVAGCVAQQEGEALLRRIPELDLVMGPQHANRLEALLTQVDNGQQVVATDDNHILED
LTTARRDSTICAWVNVIYGCNERCTYCVVPSVRGKEQSRSPEAIRLEIEGLAARGFREITLLGQNIDAYGRDLPGITPEG
RRQNTLTDLLHHIHDVEGIERIRFATSHPRYFTERLIEACFDLPKVCEHFHIPFQSGDNDVLKAMARGYTVERYRRIVNR
IRELMPDAAISTDVIVAFPGETDAQFQNTLNLVEEVGFDQVNTAAYSPRPNTPAATWSNQLPEVVKVERLKQLNALVERV
ALQRNSRYSGKVEQVLAEGINPKKPQQLMGRTRTNRLTFFAAEGPQSCRYSPGDLVDVQINSVRAFSLSGTPCDQTRSRH
>Mature_480_residues
MFRSFASPLALVATSALATNPATTAQHQGSFWIQTFGCQMNKADSERMAGILEAMGYHEAPAELEADLVLYNTCTIRDNA
EQKVYSYLGRQARRKRTHPHLKLVVAGCVAQQEGEALLRRIPELDLVMGPQHANRLEALLTQVDNGQQVVATDDNHILED
LTTARRDSTICAWVNVIYGCNERCTYCVVPSVRGKEQSRSPEAIRLEIEGLAARGFREITLLGQNIDAYGRDLPGITPEG
RRQNTLTDLLHHIHDVEGIERIRFATSHPRYFTERLIEACFDLPKVCEHFHIPFQSGDNDVLKAMARGYTVERYRRIVNR
IRELMPDAAISTDVIVAFPGETDAQFQNTLNLVEEVGFDQVNTAAYSPRPNTPAATWSNQLPEVVKVERLKQLNALVERV
ALQRNSRYSGKVEQVLAEGINPKKPQQLMGRTRTNRLTFFAAEGPQSCRYSPGDLVDVQINSVRAFSLSGTPCDQTRSRH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=475, Percent_Identity=32.8421052631579, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=32.8421052631579, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI93277076, Length=377, Percent_Identity=33.1564986737401, Blast_Score=175, Evalue=1e-43,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=39.8190045248869, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1787057, Length=446, Percent_Identity=26.0089686098655, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=422, Percent_Identity=34.3601895734597, Blast_Score=224, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI71996771, Length=380, Percent_Identity=26.5789473684211, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21356207, Length=476, Percent_Identity=31.9327731092437, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI19922432, Length=470, Percent_Identity=29.1489361702128, Blast_Score=155, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PROM3 (A2CB74)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001017999.1
- ProteinModelPortal:   A2CB74
- SMR:   A2CB74
- STRING:   A2CB74
- GeneID:   4777803
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_19921
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14333
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53827; Mature: 53827

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRSFASPLALVATSALATNPATTAQHQGSFWIQTFGCQMNKADSERMAGILEAMGYHEA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCC
PAELEADLVLYNTCTIRDNAEQKVYSYLGRQARRKRTHPHLKLVVAGCVAQQEGEALLRR
CCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHH
IPELDLVMGPQHANRLEALLTQVDNGQQVVATDDNHILEDLTTARRDSTICAWVNVIYGC
CCCCCEEECCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCC
NERCTYCVVPSVRGKEQSRSPEAIRLEIEGLAARGFREITLLGQNIDAYGRDLPGITPEG
CCCCEEEEECCCCCCCCCCCCCEEEEEEECHHHCCCEEEEEECCCCCHHCCCCCCCCCCC
RRQNTLTDLLHHIHDVEGIERIRFATSHPRYFTERLIEACFDLPKVCEHFHIPFQSGDND
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHH
VLKAMARGYTVERYRRIVNRIRELMPDAAISTDVIVAFPGETDAQFQNTLNLVEEVGFDQ
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCH
VNTAAYSPRPNTPAATWSNQLPEVVKVERLKQLNALVERVALQRNSRYSGKVEQVLAEGI
HCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
NPKKPQQLMGRTRTNRLTFFAAEGPQSCRYSPGDLVDVQINSVRAFSLSGTPCDQTRSRH
CCCCCHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCEEEEEECCCCCHHCCCCC
>Mature Secondary Structure
MFRSFASPLALVATSALATNPATTAQHQGSFWIQTFGCQMNKADSERMAGILEAMGYHEA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCC
PAELEADLVLYNTCTIRDNAEQKVYSYLGRQARRKRTHPHLKLVVAGCVAQQEGEALLRR
CCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHH
IPELDLVMGPQHANRLEALLTQVDNGQQVVATDDNHILEDLTTARRDSTICAWVNVIYGC
CCCCCEEECCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCC
NERCTYCVVPSVRGKEQSRSPEAIRLEIEGLAARGFREITLLGQNIDAYGRDLPGITPEG
CCCCEEEEECCCCCCCCCCCCCEEEEEEECHHHCCCEEEEEECCCCCHHCCCCCCCCCCC
RRQNTLTDLLHHIHDVEGIERIRFATSHPRYFTERLIEACFDLPKVCEHFHIPFQSGDND
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHH
VLKAMARGYTVERYRRIVNRIRELMPDAAISTDVIVAFPGETDAQFQNTLNLVEEVGFDQ
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCH
VNTAAYSPRPNTPAATWSNQLPEVVKVERLKQLNALVERVALQRNSRYSGKVEQVLAEGI
HCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
NPKKPQQLMGRTRTNRLTFFAAEGPQSCRYSPGDLVDVQINSVRAFSLSGTPCDQTRSRH
CCCCCHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCEEEEEECCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA