Click here to switch to the map view.

The map label for this gene is thrB

Identifier: 124023558

GI number: 124023558

Start: 1620202

End: 1621152

Strand: Reverse

Name: thrB

Synonym: P9303_18581

Alternate gene names: 124023558

Gene position: 1621152-1620202 (Counterclockwise)

Preceding gene: 124023559

Following gene: 124023557

Centisome position: 60.43

GC content: 57.2

Gene sequence:

>951_bases
ATGGCGCAGCCGAGTATCGGCCAAAAAATTGTGGTTGACGTACCGTCCACCACCGCCAACCTTGGTCCTGGCTTCGACTG
CCTTGGTGCTGCCCTTGACCTCAACAACCGTTTTGCCATGCGGCGGATCGAAGGGGACAGCGGACGCTTTGAACTCATTA
TTGAAGGCAATGAGGGAAGCCACCTACGCGGTGGGCCCAACAACCTGATTTATCGCGCCGCCCAGAGGGTGTGGAAAGCC
GCAGGGCTCGAACCGGTGGGACTAGAAGCCAAAGTGAGGCTTGCGGTACCCCCCGCAAGAGGCCTAGGAAGCAGTGCCAG
TGCCATCGTGGCAGGGTTGGTTGGAGCCAATGCCTTGGTGGGCGAACCTCTCAGCAAAGAAAAACTGTTGGAACTGGCCA
TTGACATTGAGGGACATCCCGACAATGTGGTGCCTTCTCTTCTAGGAGGTCTTTGCTTGACTGCCAAGGCAGCCTCGCAA
CGCTGGCGGGTTGTTCGTTGTGTCTGGATCAATTCCGTGAAAGTCGTTGTAGCAATCCCCTCTATTCGCCTAAGCACGAG
CGAGGCAAGGCGCGCCATGCCTAAAGACATTCCGATCAGCGATGCCGTAGAAAACCTTGGTGCCCTTACGCTCCTGCTGC
AGGGACTGCGGACAGGAAACGGCGACCTGATTACAGACGGGATGCACGATCGATTGCATGAGCCCTATCGCTGGCCATTA
ATCAAAGGTGGTTTGGATGTTCGCGATGCGGCTCTGAATGCCGGGGCCTGGGGTTGTGCCATCAGCGGAGCTGGCCCCAG
CGTGCTGGCTCTGTGCCCGGAGGATAAAGGGCAAGCAGTCAGTCAGGCAATGGTAAAAGCTTGGGAGGCCGAGGGTGTAG
CAAGTAGGGCACCACTGCTTAGCATTCAGACAGGAGGGAGCCACTGGCAACCTCAAATTGAGGATGAGTAG

Upstream 100 bases:

>100_bases
TGGCAGTGATCGATCCCGAGGTGGGCCTGTTCAGTGCAGCCTGCAGAGCACACATGCTTGCTGAGCAAGGTGGGACACTG
ACCTAAGTAGACAAGTAGGG

Downstream 100 bases:

>100_bases
ATATACTCAAGCGCCCACCCCGGGGCTGATCAGTTCCTTCGCATTCTCTTGCCTGCCATGGACGCCAATTTGCCGCTGAC
GGCTGCGTCCCAGGCCTCAT

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MAQPSIGQKIVVDVPSTTANLGPGFDCLGAALDLNNRFAMRRIEGDSGRFELIIEGNEGSHLRGGPNNLIYRAAQRVWKA
AGLEPVGLEAKVRLAVPPARGLGSSASAIVAGLVGANALVGEPLSKEKLLELAIDIEGHPDNVVPSLLGGLCLTAKAASQ
RWRVVRCVWINSVKVVVAIPSIRLSTSEARRAMPKDIPISDAVENLGALTLLLQGLRTGNGDLITDGMHDRLHEPYRWPL
IKGGLDVRDAALNAGAWGCAISGAGPSVLALCPEDKGQAVSQAMVKAWEAEGVASRAPLLSIQTGGSHWQPQIEDE

Sequences:

>Translated_316_residues
MAQPSIGQKIVVDVPSTTANLGPGFDCLGAALDLNNRFAMRRIEGDSGRFELIIEGNEGSHLRGGPNNLIYRAAQRVWKA
AGLEPVGLEAKVRLAVPPARGLGSSASAIVAGLVGANALVGEPLSKEKLLELAIDIEGHPDNVVPSLLGGLCLTAKAASQ
RWRVVRCVWINSVKVVVAIPSIRLSTSEARRAMPKDIPISDAVENLGALTLLLQGLRTGNGDLITDGMHDRLHEPYRWPL
IKGGLDVRDAALNAGAWGCAISGAGPSVLALCPEDKGQAVSQAMVKAWEAEGVASRAPLLSIQTGGSHWQPQIEDE
>Mature_315_residues
AQPSIGQKIVVDVPSTTANLGPGFDCLGAALDLNNRFAMRRIEGDSGRFELIIEGNEGSHLRGGPNNLIYRAAQRVWKAA
GLEPVGLEAKVRLAVPPARGLGSSASAIVAGLVGANALVGEPLSKEKLLELAIDIEGHPDNVVPSLLGGLCLTAKAASQR
WRVVRCVWINSVKVVVAIPSIRLSTSEARRAMPKDIPISDAVENLGALTLLLQGLRTGNGDLITDGMHDRLHEPYRWPLI
KGGLDVRDAALNAGAWGCAISGAGPSVLALCPEDKGQAVSQAMVKAWEAEGVASRAPLLSIQTGGSHWQPQIEDE

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=281, Percent_Identity=30.9608540925267, Blast_Score=105, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321814, Length=356, Percent_Identity=30.0561797752809, Blast_Score=142, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_PROM3 (A2CAU0)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001017865.1
- ProteinModelPortal:   A2CAU0
- SMR:   A2CAU0
- STRING:   A2CAU0
- GeneID:   4775974
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_18581
- eggNOG:   COG0083
- HOGENOM:   HBG646290
- OMA:   FDCLGAA
- ProtClustDB:   PRK01212
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 33249; Mature: 33117

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPSIGQKIVVDVPSTTANLGPGFDCLGAALDLNNRFAMRRIEGDSGRFELIIEGNEGS
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCC
HLRGGPNNLIYRAAQRVWKAAGLEPVGLEAKVRLAVPPARGLGSSASAIVAGLVGANALV
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCHHH
GEPLSKEKLLELAIDIEGHPDNVVPSLLGGLCLTAKAASQRWRVVRCVWINSVKVVVAIP
CCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEEEC
SIRLSTSEARRAMPKDIPISDAVENLGALTLLLQGLRTGNGDLITDGMHDRLHEPYRWPL
CEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHCCCCCCCE
IKGGLDVRDAALNAGAWGCAISGAGPSVLALCPEDKGQAVSQAMVKAWEAEGVASRAPLL
EECCCCHHHHHHCCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEE
SIQTGGSHWQPQIEDE
EEECCCCCCCCCCCCC
>Mature Secondary Structure 
AQPSIGQKIVVDVPSTTANLGPGFDCLGAALDLNNRFAMRRIEGDSGRFELIIEGNEGS
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCC
HLRGGPNNLIYRAAQRVWKAAGLEPVGLEAKVRLAVPPARGLGSSASAIVAGLVGANALV
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCHHH
GEPLSKEKLLELAIDIEGHPDNVVPSLLGGLCLTAKAASQRWRVVRCVWINSVKVVVAIP
CCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEEEC
SIRLSTSEARRAMPKDIPISDAVENLGALTLLLQGLRTGNGDLITDGMHDRLHEPYRWPL
CEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHCCCCCCCE
IKGGLDVRDAALNAGAWGCAISGAGPSVLALCPEDKGQAVSQAMVKAWEAEGVASRAPLL
EECCCCHHHHHHCCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEE
SIQTGGSHWQPQIEDE
EEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA