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The map label for this gene is clpB [H]

Identifier: 124023532

GI number: 124023532

Start: 1589658

End: 1592249

Strand: Reverse

Name: clpB [H]

Synonym: P9303_18321

Alternate gene names: 124023532

Gene position: 1592249-1589658 (Counterclockwise)

Preceding gene: 124023533

Following gene: 124023530

Centisome position: 59.35

GC content: 50.66

Gene sequence:

>2592_bases
ATGCAACCTACGGCGGATCAATTTACCGAAAAAGGCTGGGCAGCAATCGTTTTGGCCCAACAACTTGCCCAGCAACGCAA
GCACCAGCAACTGGAAACAGAGCATTTGTTGTTGTCACTGCTAGAGCAAAATGCTTTGGCAGGAAGAATCCTAGAAAAAG
CAGGTGTGTCGATCGGCAACTTGCAAACAGCCGTGGAGGCACACCTGCATGAACAGCCCACTCTGCAGGCAGCGCCAGAC
TCTGTCTATCTGGGCAAAGGAGTCAATGATCTGCTCGATCAAGCTGATAAACATAAGCAAGCTTTTGGCGATGGTTTCAT
CTCAATTGAACATCTGTTACTTGCATTGGCCGGGGACAATCGTTGCGGCCGCAAGCTGCTCAATCAAGCGGGCGTGGATG
CGGGCAAACTAAAGGTTGCAATTGACGCGGTACGCGGAAACCAGAAGGTGACCGATCAGAACCCAGAAGGAACTTACGAA
TCTCTCGAAAAATATGGCAGAGACCTGACTGCTGCTGCTCGCGAAGGCAAACTCGACCCTGTCATCGGAAGGGACGATGA
GATTCGACGCACGATTCAGATTCTTAGTAGGCGCACCAAAAACAACCCTGTCCTCATTGGGGAGCCAGGGGTGGGAAAAA
CCGCAATTGTTGAAGGCCTCGCACAACGGATTGTTAATGGCGATGTACCTGCAGCCCTTCAGAACAGACAGTTAATCGCC
CTTGATATGGGTGCTTTGATTGCAGGGGCAAAATATCGAGGTGAATTCGAAGAGCGACTCAAGGCAGTGCTCAAAGAAGT
TACCGCCTCTGAAGGACAAATCGTACTTTTTATTGATGAAATCCATACCGTTGTAGGAGCTGGAGCTACCGGTGGGGCAA
TGGATGCCAGCAATCTGCTTAAGCCAATGCTGGCTCGAGGGGAACTGCGCTGCATTGGTGCAACGACCCTCGATGAGCAC
CGACAGCACATCGAAAAAGACCCCGCTTTAGAAAGAAGATTCCAGCAAGTGCTTGTGGATCAACCCACCGTGCAAGACAC
GATCTCCATCCTGCGTGGTCTCAAAGAACGCTACGAAGTGCATCATGGTGTGCGCATTGCTGATAACGCCCTGGTTGCTG
CAGCTGTGCTCAGTAGCCGATATATTGCCGATCGTTTTTTGCCAGATAAAGCCATCGATTTGATGGATGAATCTGCTGCA
CGACTAAAAATGGAAATCACCTCCAAGCCGGAGGAAATCGATGAAATCGATCGCAAGATTGTGCAGCTCGAGATGGAGAA
GCTTTCCTTAGGACGTGAGTCCGACTCCGTTAGTAAGGAAAGATTGGAAAAACTAGAACGCGAGCTTGCTGAATTAGCAG
AGCAACAAAGTGCGCTCAATGCACAGTGGCAACAAGAAAAAGGTGCGATTGACGACCTTTCTTCGCTCAAAGAAGAAATC
GAAAGGGTGCAATTGCAAGTTGAGCAAGCCAAGCGCAGCTACGACCTCAACAAAGCAGCTGAACTGGAGTACGGAACTCT
GGCGGGATTGCAGAAACAACTCAGCGAGAAAGAAACTGCCCTGGCCCAAGATGGAGAGGCAGGCGATAAATCACTGCTGC
GAGAGGAGGTTACGGAAGACGATATTGCTGATGTCATCGCTAAATGGACCGGGATTCCTGTCGCCAAGCTTGTGCAGTCG
GAAATGGAAAAGCTGCTGGGACTTGAAGCAGAACTCCACCAACGTGTGATTGGCCAAGAACAGGCTGTGCAAGCTGTTGC
GGATGCGATTCAGCGTTCTAGGGCAGGTTTAAGTGATCCCAACCGCCCGATCGCAAGTTTTTTATTTCTAGGCCCCACTG
GTGTAGGTAAAACGGAGCTATCCAAGGCATTGGCCTCTCAACTGTTTGACAGCGAGGCGGCTTTGGTGCGAATCGATATG
TCTGAGTATATGGAGAAGCACAGTGTAAGCAGACTGATCGGGGCACCCCCGGGTTATGTCGGCTATGAGGCTGGTGGACA
GCTCACCGAAGCCGTACGCAGACGCCCTTATGCGGTAATCCTGTTCGATGAAGTGGAGAAGGCACACCCAGATGTGTTCA
ATGTGATGTTACAGATCCTCGATGATGGACGTGTTACCGATGGTCAGGGTCGCACAGTTGATTTTACAAATACGGTGCTA
ATTCTTACCAGTAATATTGGCAGCCAGTCGATTCTCGACTTGGGAGGCGATGATAGTCAATACGGGGAAATGGAGCGTCG
AGTTCATGATGCTTTGCACGCTCATTTTAGGCCTGAGTTTCTCAACCGCCTGGATGAAACAATCATCTTTCATAGCCTCA
GGCGCGAGGAACTGCGTCAGATCGTTGCCCTTCAGGTCAATCGGCTGCGTGAGCGTCTTGGCGATCGCAAGCTTGGCCTA
GAGATCAGTGATACAGCAGCTGATTGGCTCGCTAATGCTGGCTATGACCCTGTTTATGGGGCCAGACCCCTCAAGCGAGC
GATCCAACGCGAGCTTGAAACTCCAATAGCCAAAAGTATCCTGGCTGGCTTTTATGGAGATAGTCAGATTGTGCATGTGG
ATGTGGACGAGGAGCGTCTGAGCTTTCGATAA

Upstream 100 bases:

>100_bases
GCTTACCACACTCTGCCGAAAGAGCCGTACCAATCAGAACGGTTTACCGCTTGGCGCTAATGGTTAGTCGACATACGGTT
GAATCAACGTAAAAGACCAC

Downstream 100 bases:

>100_bases
CCCTCCGTTCATTAGCTCGCCCACCAGGGGAGTCGATCGAGCAAATTGATCAGAGCAGAGTTTTGCTCTCATACCACTGA
GGTATTGCTTCGTAGCAAGC

Product: ATP-dependent Clp protease Hsp 100, ATP-binding subunit ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 863; Mature: 863

Protein sequence:

>863_residues
MQPTADQFTEKGWAAIVLAQQLAQQRKHQQLETEHLLLSLLEQNALAGRILEKAGVSIGNLQTAVEAHLHEQPTLQAAPD
SVYLGKGVNDLLDQADKHKQAFGDGFISIEHLLLALAGDNRCGRKLLNQAGVDAGKLKVAIDAVRGNQKVTDQNPEGTYE
SLEKYGRDLTAAAREGKLDPVIGRDDEIRRTIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGDVPAALQNRQLIA
LDMGALIAGAKYRGEFEERLKAVLKEVTASEGQIVLFIDEIHTVVGAGATGGAMDASNLLKPMLARGELRCIGATTLDEH
RQHIEKDPALERRFQQVLVDQPTVQDTISILRGLKERYEVHHGVRIADNALVAAAVLSSRYIADRFLPDKAIDLMDESAA
RLKMEITSKPEEIDEIDRKIVQLEMEKLSLGRESDSVSKERLEKLERELAELAEQQSALNAQWQQEKGAIDDLSSLKEEI
ERVQLQVEQAKRSYDLNKAAELEYGTLAGLQKQLSEKETALAQDGEAGDKSLLREEVTEDDIADVIAKWTGIPVAKLVQS
EMEKLLGLEAELHQRVIGQEQAVQAVADAIQRSRAGLSDPNRPIASFLFLGPTGVGKTELSKALASQLFDSEAALVRIDM
SEYMEKHSVSRLIGAPPGYVGYEAGGQLTEAVRRRPYAVILFDEVEKAHPDVFNVMLQILDDGRVTDGQGRTVDFTNTVL
ILTSNIGSQSILDLGGDDSQYGEMERRVHDALHAHFRPEFLNRLDETIIFHSLRREELRQIVALQVNRLRERLGDRKLGL
EISDTAADWLANAGYDPVYGARPLKRAIQRELETPIAKSILAGFYGDSQIVHVDVDEERLSFR

Sequences:

>Translated_863_residues
MQPTADQFTEKGWAAIVLAQQLAQQRKHQQLETEHLLLSLLEQNALAGRILEKAGVSIGNLQTAVEAHLHEQPTLQAAPD
SVYLGKGVNDLLDQADKHKQAFGDGFISIEHLLLALAGDNRCGRKLLNQAGVDAGKLKVAIDAVRGNQKVTDQNPEGTYE
SLEKYGRDLTAAAREGKLDPVIGRDDEIRRTIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGDVPAALQNRQLIA
LDMGALIAGAKYRGEFEERLKAVLKEVTASEGQIVLFIDEIHTVVGAGATGGAMDASNLLKPMLARGELRCIGATTLDEH
RQHIEKDPALERRFQQVLVDQPTVQDTISILRGLKERYEVHHGVRIADNALVAAAVLSSRYIADRFLPDKAIDLMDESAA
RLKMEITSKPEEIDEIDRKIVQLEMEKLSLGRESDSVSKERLEKLERELAELAEQQSALNAQWQQEKGAIDDLSSLKEEI
ERVQLQVEQAKRSYDLNKAAELEYGTLAGLQKQLSEKETALAQDGEAGDKSLLREEVTEDDIADVIAKWTGIPVAKLVQS
EMEKLLGLEAELHQRVIGQEQAVQAVADAIQRSRAGLSDPNRPIASFLFLGPTGVGKTELSKALASQLFDSEAALVRIDM
SEYMEKHSVSRLIGAPPGYVGYEAGGQLTEAVRRRPYAVILFDEVEKAHPDVFNVMLQILDDGRVTDGQGRTVDFTNTVL
ILTSNIGSQSILDLGGDDSQYGEMERRVHDALHAHFRPEFLNRLDETIIFHSLRREELRQIVALQVNRLRERLGDRKLGL
EISDTAADWLANAGYDPVYGARPLKRAIQRELETPIAKSILAGFYGDSQIVHVDVDEERLSFR
>Mature_863_residues
MQPTADQFTEKGWAAIVLAQQLAQQRKHQQLETEHLLLSLLEQNALAGRILEKAGVSIGNLQTAVEAHLHEQPTLQAAPD
SVYLGKGVNDLLDQADKHKQAFGDGFISIEHLLLALAGDNRCGRKLLNQAGVDAGKLKVAIDAVRGNQKVTDQNPEGTYE
SLEKYGRDLTAAAREGKLDPVIGRDDEIRRTIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGDVPAALQNRQLIA
LDMGALIAGAKYRGEFEERLKAVLKEVTASEGQIVLFIDEIHTVVGAGATGGAMDASNLLKPMLARGELRCIGATTLDEH
RQHIEKDPALERRFQQVLVDQPTVQDTISILRGLKERYEVHHGVRIADNALVAAAVLSSRYIADRFLPDKAIDLMDESAA
RLKMEITSKPEEIDEIDRKIVQLEMEKLSLGRESDSVSKERLEKLERELAELAEQQSALNAQWQQEKGAIDDLSSLKEEI
ERVQLQVEQAKRSYDLNKAAELEYGTLAGLQKQLSEKETALAQDGEAGDKSLLREEVTEDDIADVIAKWTGIPVAKLVQS
EMEKLLGLEAELHQRVIGQEQAVQAVADAIQRSRAGLSDPNRPIASFLFLGPTGVGKTELSKALASQLFDSEAALVRIDM
SEYMEKHSVSRLIGAPPGYVGYEAGGQLTEAVRRRPYAVILFDEVEKAHPDVFNVMLQILDDGRVTDGQGRTVDFTNTVL
ILTSNIGSQSILDLGGDDSQYGEMERRVHDALHAHFRPEFLNRLDETIIFHSLRREELRQIVALQVNRLRERLGDRKLGL
EISDTAADWLANAGYDPVYGARPLKRAIQRELETPIAKSILAGFYGDSQIVHVDVDEERLSFR

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=301, Percent_Identity=39.5348837209302, Blast_Score=206, Evalue=9e-53,
Organism=Escherichia coli, GI1788943, Length=856, Percent_Identity=56.0747663551402, Blast_Score=946, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=408, Percent_Identity=40.9313725490196, Blast_Score=282, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=713, Percent_Identity=53.015427769986, Blast_Score=774, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=859, Percent_Identity=43.3061699650757, Blast_Score=689, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95356; Mature: 95356

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPTADQFTEKGWAAIVLAQQLAQQRKHQQLETEHLLLSLLEQNALAGRILEKAGVSIGN
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LQTAVEAHLHEQPTLQAAPDSVYLGKGVNDLLDQADKHKQAFGDGFISIEHLLLALAGDN
HHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
RCGRKLLNQAGVDAGKLKVAIDAVRGNQKVTDQNPEGTYESLEKYGRDLTAAAREGKLDP
HHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCCCC
VIGRDDEIRRTIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGDVPAALQNRQLIA
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHCCCEEEE
LDMGALIAGAKYRGEFEERLKAVLKEVTASEGQIVLFIDEIHTVVGAGATGGAMDASNLL
EEHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHH
KPMLARGELRCIGATTLDEHRQHIEKDPALERRFQQVLVDQPTVQDTISILRGLKERYEV
HHHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HHGVRIADNALVAAAVLSSRYIADRFLPDKAIDLMDESAARLKMEITSKPEEIDEIDRKI
HCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHH
VQLEMEKLSLGRESDSVSKERLEKLERELAELAEQQSALNAQWQQEKGAIDDLSSLKEEI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHH
ERVQLQVEQAKRSYDLNKAAELEYGTLAGLQKQLSEKETALAQDGEAGDKSLLREEVTED
HHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHH
DIADVIAKWTGIPVAKLVQSEMEKLLGLEAELHQRVIGQEQAVQAVADAIQRSRAGLSDP
HHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCC
NRPIASFLFLGPTGVGKTELSKALASQLFDSEAALVRIDMSEYMEKHSVSRLIGAPPGYV
CCHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC
GYEAGGQLTEAVRRRPYAVILFDEVEKAHPDVFNVMLQILDDGRVTDGQGRTVDFTNTVL
CCCCCCHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEE
ILTSNIGSQSILDLGGDDSQYGEMERRVHDALHAHFRPEFLNRLDETIIFHSLRREELRQ
EEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IVALQVNRLRERLGDRKLGLEISDTAADWLANAGYDPVYGARPLKRAIQRELETPIAKSI
HHHHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCHHHHHH
LAGFYGDSQIVHVDVDEERLSFR
HHHHCCCCEEEEEECCHHHHCCC
>Mature Secondary Structure
MQPTADQFTEKGWAAIVLAQQLAQQRKHQQLETEHLLLSLLEQNALAGRILEKAGVSIGN
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LQTAVEAHLHEQPTLQAAPDSVYLGKGVNDLLDQADKHKQAFGDGFISIEHLLLALAGDN
HHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
RCGRKLLNQAGVDAGKLKVAIDAVRGNQKVTDQNPEGTYESLEKYGRDLTAAAREGKLDP
HHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCCCC
VIGRDDEIRRTIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGDVPAALQNRQLIA
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHCCCEEEE
LDMGALIAGAKYRGEFEERLKAVLKEVTASEGQIVLFIDEIHTVVGAGATGGAMDASNLL
EEHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHH
KPMLARGELRCIGATTLDEHRQHIEKDPALERRFQQVLVDQPTVQDTISILRGLKERYEV
HHHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HHGVRIADNALVAAAVLSSRYIADRFLPDKAIDLMDESAARLKMEITSKPEEIDEIDRKI
HCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHH
VQLEMEKLSLGRESDSVSKERLEKLERELAELAEQQSALNAQWQQEKGAIDDLSSLKEEI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHH
ERVQLQVEQAKRSYDLNKAAELEYGTLAGLQKQLSEKETALAQDGEAGDKSLLREEVTED
HHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHH
DIADVIAKWTGIPVAKLVQSEMEKLLGLEAELHQRVIGQEQAVQAVADAIQRSRAGLSDP
HHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCC
NRPIASFLFLGPTGVGKTELSKALASQLFDSEAALVRIDMSEYMEKHSVSRLIGAPPGYV
CCHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC
GYEAGGQLTEAVRRRPYAVILFDEVEKAHPDVFNVMLQILDDGRVTDGQGRTVDFTNTVL
CCCCCCHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEE
ILTSNIGSQSILDLGGDDSQYGEMERRVHDALHAHFRPEFLNRLDETIIFHSLRREELRQ
EEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IVALQVNRLRERLGDRKLGLEISDTAADWLANAGYDPVYGARPLKRAIQRELETPIAKSI
HHHHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCHHHHHH
LAGFYGDSQIVHVDVDEERLSFR
HHHHCCCCEEEEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]