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The map label for this gene is trpA [H]

Identifier: 124023516

GI number: 124023516

Start: 1580586

End: 1581422

Strand: Direct

Name: trpA [H]

Synonym: P9303_18161

Alternate gene names: 124023516

Gene position: 1580586-1581422 (Clockwise)

Preceding gene: 124023515

Following gene: 124023518

Centisome position: 58.92

GC content: 53.17

Gene sequence:

>837_bases
ATGATTGCTATGGCAGATTTCTTGGCACCTAAATCCAATACACAAGCCACAATCAGCAGTCGCTTCCAACGAGTACAAGC
TGAGGGACGCCTTGCACTCATGCCTTTCCTAATGGCTGGTGATCCTGATCTGGCAACCACTGCTGAGGTATTGCTCAGCC
TGGAGCAATCTGGCGCGGACATGATCGAGCTGGGCATTCCCTATAGCGATCCTCTCGCCGATGGTCCGGTGATTCAGGCA
GCTGCTTCTCGAGCACTGGCTTCTGGTACGACACCAGCTCGGGTCTTACAGATGTTGATTGACTTGCGGGGCAAACTTTC
CATTCCTGTGATCCTGTTCACCTACACCAATCCATTGTTCAACCGGGGCATGGAGCGTTTCTGTGATGAAGCGGCTGAGG
CTGGAGTTGCCGGTCTTGTGGTCCCAGATCTTCCACTCGAGGAAGCTGAGCGTCTTTCTCCCTTGGCTTCTGCTCGTGGA
CTTGATCTGGTTCTTCTGGTGGCTCCTACTACACCTGCGGAGCGTATGGCAAGGATTGCAGAAAGCAGCAGGGGATTTAC
CTATTTAGTAAGCGTGACGGGAGTTACTGGAGAACGTTCTGTCATGGAGGAACGTGTTGAATCCTTGGTGCAGCAACTCA
AATTGTCCGGCTCCAATCCTGTAGCAGTCGGATTTGGAATTTCTGGGCCTCAGCAGGTACGGCAGGTGCGAAGTTGGGGT
GCTGATGGAGCCATTGTTGGCAGTGCTCTAGTGAAACGAATGGCTGCTGCGGCTCCTGGATTTGTTGCTCAAGAAGCGGG
TCTTTTCTGCAAAGAGCTGCGCAACGCTGCTGGATGA

Upstream 100 bases:

>100_bases
AGTTTCCTTACTGAAGGATATTGAGGATGAAAATCCTAAGCAACAAGCCCCCTCTGACCCATAGGCTCTAGGGCGTTTGC
CCCCCTCACGCCTTCTTCTA

Downstream 100 bases:

>100_bases
GTTTTAACTTGCTCATATCTATCATCCTGCCTTAAAACATCAACAAAAAAGCCCTGGAAAATTTCCAGGGCTTGAGTGAT
TCAAATGAGGTTGGAACGAT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MIAMADFLAPKSNTQATISSRFQRVQAEGRLALMPFLMAGDPDLATTAEVLLSLEQSGADMIELGIPYSDPLADGPVIQA
AASRALASGTTPARVLQMLIDLRGKLSIPVILFTYTNPLFNRGMERFCDEAAEAGVAGLVVPDLPLEEAERLSPLASARG
LDLVLLVAPTTPAERMARIAESSRGFTYLVSVTGVTGERSVMEERVESLVQQLKLSGSNPVAVGFGISGPQQVRQVRSWG
ADGAIVGSALVKRMAAAAPGFVAQEAGLFCKELRNAAG

Sequences:

>Translated_278_residues
MIAMADFLAPKSNTQATISSRFQRVQAEGRLALMPFLMAGDPDLATTAEVLLSLEQSGADMIELGIPYSDPLADGPVIQA
AASRALASGTTPARVLQMLIDLRGKLSIPVILFTYTNPLFNRGMERFCDEAAEAGVAGLVVPDLPLEEAERLSPLASARG
LDLVLLVAPTTPAERMARIAESSRGFTYLVSVTGVTGERSVMEERVESLVQQLKLSGSNPVAVGFGISGPQQVRQVRSWG
ADGAIVGSALVKRMAAAAPGFVAQEAGLFCKELRNAAG
>Mature_278_residues
MIAMADFLAPKSNTQATISSRFQRVQAEGRLALMPFLMAGDPDLATTAEVLLSLEQSGADMIELGIPYSDPLADGPVIQA
AASRALASGTTPARVLQMLIDLRGKLSIPVILFTYTNPLFNRGMERFCDEAAEAGVAGLVVPDLPLEEAERLSPLASARG
LDLVLLVAPTTPAERMARIAESSRGFTYLVSVTGVTGERSVMEERVESLVQQLKLSGSNPVAVGFGISGPQQVRQVRSWG
ADGAIVGSALVKRMAAAAPGFVAQEAGLFCKELRNAAG

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=234, Percent_Identity=39.3162393162393, Blast_Score=156, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321412, Length=258, Percent_Identity=37.2093023255814, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 29256; Mature: 29256

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAMADFLAPKSNTQATISSRFQRVQAEGRLALMPFLMAGDPDLATTAEVLLSLEQSGAD
CCCCHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHCCCCCHHHHHHHHHHHHHCCCC
MIELGIPYSDPLADGPVIQAAASRALASGTTPARVLQMLIDLRGKLSIPVILFTYTNPLF
EEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHH
NRGMERFCDEAAEAGVAGLVVPDLPLEEAERLSPLASARGLDLVLLVAPTTPAERMARIA
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCHHHHCCCCEEEEEEECCCCHHHHHHHH
ESSRGFTYLVSVTGVTGERSVMEERVESLVQQLKLSGSNPVAVGFGISGPQQVRQVRSWG
HCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCC
ADGAIVGSALVKRMAAAAPGFVAQEAGLFCKELRNAAG
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIAMADFLAPKSNTQATISSRFQRVQAEGRLALMPFLMAGDPDLATTAEVLLSLEQSGAD
CCCCHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHCCCCCHHHHHHHHHHHHHCCCC
MIELGIPYSDPLADGPVIQAAASRALASGTTPARVLQMLIDLRGKLSIPVILFTYTNPLF
EEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHH
NRGMERFCDEAAEAGVAGLVVPDLPLEEAERLSPLASARGLDLVLLVAPTTPAERMARIA
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCHHHHCCCCEEEEEEECCCCHHHHHHHH
ESSRGFTYLVSVTGVTGERSVMEERVESLVQQLKLSGSNPVAVGFGISGPQQVRQVRSWG
HCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCC
ADGAIVGSALVKRMAAAAPGFVAQEAGLFCKELRNAAG
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]