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The map label for this gene is hofD [H]

Identifier: 124023432

GI number: 124023432

Start: 1514798

End: 1515574

Strand: Reverse

Name: hofD [H]

Synonym: P9303_17321

Alternate gene names: 124023432

Gene position: 1515574-1514798 (Counterclockwise)

Preceding gene: 124023433

Following gene: 124023431

Centisome position: 56.49

GC content: 55.08

Gene sequence:

>777_bases
ATGGGCAGCTTCCTCAATGTGATGGCCTTGCGGCTTCCCCGAAAGGAATCATTGATTTGGCCTGGTAGTCATTGCCCACG
TTGCGGATGCGGTGTGCGTTGGTACGACAACATCCCAGTACTCGCATGGATTCAACTAGGCGGTCGCTGTCGTGATTGCA
GAACCACCATTCCAGTGCGTTATCCAGCTGTGGAGAGTCTCAGCGCCGGGCTTTGGGTATCGGCTGCCTTGGCAGCTCCA
ACCAGCATGGGCGAGTTGTCTTCCTTGTTCAATCTCTTTGCTGGAGTAGTGCTTGTCAGTGTGCTGTTACCACTGGTCCT
CATCGACCTCGATCACATGTGGTTGCCAGAACCACTCTGTCGGGCTGGCCTTTGGTTAGGCCTGGCTTTCACAGCGACAG
CGGCAGTTTCACTCGGCTGGAGTAAGGGATCAGACCTGCTGCTCGACCATTTACTAGCAGCAGCAGCAGCCTTAATTGCG
ATGGAAGGGCTTAGCTTCCTTTCCAAAAAGCTGATTGGTCAGCCAGCCCTTGGGCTTGGTGATGCCAAATTGGCGGCCTT
GGCTGGAGCATGGTTAGGACTGAAAGGGGTGGCTATGGCCATGACCCTGGCCATCATCAGCGGCGCAACGGTTGGTCTCA
TCGGTCGGCTTACTGGTCAGCTACAACCTCGCCAGCCTTTTCCCTTCGGGCCTTTCATTGCCTTTGGGGTCTGGGGTGTA
TGGCTTTGCGGATCAGGTTGGTGGTGGCAACATTGGTTTGACCTAATGGGTCTTTAA

Upstream 100 bases:

>100_bases
GCACCCTCCCGATGCGCTCCCGTCAAACCCGCGCAAGAAACATCGCCATCTCTAGGCGGAGCTAATGCTCTGGATCTATC
TGGCGATCATCGGAGCTTGT

Downstream 100 bases:

>100_bases
TAGACGAAAGGCTGCTAGAAAAAAATGCCAACGGTCAGTGAGTGAAGCGCGCTTTAGATTTGGATGATTCACCACTTCTT
AATTTGCGGTATTGGACATA

Product: type 4 prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MGSFLNVMALRLPRKESLIWPGSHCPRCGCGVRWYDNIPVLAWIQLGGRCRDCRTTIPVRYPAVESLSAGLWVSAALAAP
TSMGELSSLFNLFAGVVLVSVLLPLVLIDLDHMWLPEPLCRAGLWLGLAFTATAAVSLGWSKGSDLLLDHLLAAAAALIA
MEGLSFLSKKLIGQPALGLGDAKLAALAGAWLGLKGVAMAMTLAIISGATVGLIGRLTGQLQPRQPFPFGPFIAFGVWGV
WLCGSGWWWQHWFDLMGL

Sequences:

>Translated_258_residues
MGSFLNVMALRLPRKESLIWPGSHCPRCGCGVRWYDNIPVLAWIQLGGRCRDCRTTIPVRYPAVESLSAGLWVSAALAAP
TSMGELSSLFNLFAGVVLVSVLLPLVLIDLDHMWLPEPLCRAGLWLGLAFTATAAVSLGWSKGSDLLLDHLLAAAAALIA
MEGLSFLSKKLIGQPALGLGDAKLAALAGAWLGLKGVAMAMTLAIISGATVGLIGRLTGQLQPRQPFPFGPFIAFGVWGV
WLCGSGWWWQHWFDLMGL
>Mature_257_residues
GSFLNVMALRLPRKESLIWPGSHCPRCGCGVRWYDNIPVLAWIQLGGRCRDCRTTIPVRYPAVESLSAGLWVSAALAAPT
SMGELSSLFNLFAGVVLVSVLLPLVLIDLDHMWLPEPLCRAGLWLGLAFTATAAVSLGWSKGSDLLLDHLLAAAAALIAM
EGLSFLSKKLIGQPALGLGDAKLAALAGAWLGLKGVAMAMTLAIISGATVGLIGRLTGQLQPRQPFPFGPFIAFGVWGVW
LCGSGWWWQHWFDLMGL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=255, Percent_Identity=32.5490196078431, Blast_Score=104, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 27605; Mature: 27474

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSFLNVMALRLPRKESLIWPGSHCPRCGCGVRWYDNIPVLAWIQLGGRCRDCRTTIPVR
CCHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEEECCCCEEEEEHHCCCCCCCCCCCCEE
YPAVESLSAGLWVSAALAAPTSMGELSSLFNLFAGVVLVSVLLPLVLIDLDHMWLPEPLC
CCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
RAGLWLGLAFTATAAVSLGWSKGSDLLLDHLLAAAAALIAMEGLSFLSKKLIGQPALGLG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DAKLAALAGAWLGLKGVAMAMTLAIISGATVGLIGRLTGQLQPRQPFPFGPFIAFGVWGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
WLCGSGWWWQHWFDLMGL
HHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
GSFLNVMALRLPRKESLIWPGSHCPRCGCGVRWYDNIPVLAWIQLGGRCRDCRTTIPVR
CHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEEECCCCEEEEEHHCCCCCCCCCCCCEE
YPAVESLSAGLWVSAALAAPTSMGELSSLFNLFAGVVLVSVLLPLVLIDLDHMWLPEPLC
CCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
RAGLWLGLAFTATAAVSLGWSKGSDLLLDHLLAAAAALIAMEGLSFLSKKLIGQPALGLG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DAKLAALAGAWLGLKGVAMAMTLAIISGATVGLIGRLTGQLQPRQPFPFGPFIAFGVWGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
WLCGSGWWWQHWFDLMGL
HHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]