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The map label for this gene is proW [H]

Identifier: 124023400

GI number: 124023400

Start: 1485369

End: 1486283

Strand: Reverse

Name: proW [H]

Synonym: P9303_16981

Alternate gene names: 124023400

Gene position: 1486283-1485369 (Counterclockwise)

Preceding gene: 124023401

Following gene: 124023399

Centisome position: 55.4

GC content: 51.8

Gene sequence:

>915_bases
ATGATGGATGTGATTGACCAAGTTTTTGCAACCGTCAGCACCGGCCCTGTAGGCGAGGGCATGAGCAGATTCGTGGAATG
GTTGCTCAATCATGCGCAACCAATTTTCCTAGTGATTGATTCTGCAATTAATGGGCTAGCCGGAGCAATCGAACAGATAC
TCTCCGTGCCAGCGCCCTGGCTGCTAGCTCCCCTGATCGCCATACTCGCAGCTTGGCGAGTGAGTTTGAGCTTCGCCATT
CTCAGCCTTCTTGGTCTGAACCTGGTTTTGTTCATGGGGCTTTGGCAGCCAATGATCTCAACCCTGGCCCTAGTTATTGC
AGCCTCATTACTGGCACTAATCATTGGCATCCCGATCGGCATTTTTTCTGCCCGTCGCCAACACATCTGGGCGATTACCC
GCCCAGTATTGGATCTAATGCAAACCATGCCGGCATTCGTTTATCTCATTCCGGCAGTGATGTTTTTTGGCACAGGGCTT
GTCCCATCCACTATCGCAACGCTGATCTTCTCGATGCCACCCGTAGTACGCCTGACGTACCTGGGCATACGACAAGTCCC
TGTTGATCTAATCGAAGCCGGGCGCGCCTTCGGTTGCTCAGAACGACAATTGCTCTGGAAAGTGCAACTTCCAAACGCCT
TACCAACCTTGATGGCTGGTGTTAATCAAACCATCATGCTCGCTCTATCGATGGTTGTTATCGCATCAATGATTGGTGGT
GGCGGCCTAGGTGATGTGGTGCTAAGAGGCATCCAACAACTTGACGTAGGCCTTGGCTTCGAGGGTGGGCTCGCCGTGGT
AATTCTGGCTGTAATCCTGGATCGCCTCACACAAAGCCTGGCTGCCTCAAACTTTTCGAGAAAGTCATTGCCGCAGCGCT
TTAAAGCCTTTACTAACCTCTGGACTTCCTCATGA

Upstream 100 bases:

>100_bases
CTGTTGCCAACGCCCCGTATCCACTTCCAGTGATTGGATCAGATCAGCGCCTCATTGGCGTGATTAGTCCACGTCGACTT
TTGCGCTCGATGATCCTGAG

Downstream 100 bases:

>100_bases
AAATGTCTGACAAGTTGCGAAATCTAAGCCTTGTTATCGCTGGAGTGCTGATGACTGGCTTGTCGGGATGTTCGAACGCC
AAACCAGATCAATTGCGCAT

Product: glycine/bertaine ABC transporter permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MMDVIDQVFATVSTGPVGEGMSRFVEWLLNHAQPIFLVIDSAINGLAGAIEQILSVPAPWLLAPLIAILAAWRVSLSFAI
LSLLGLNLVLFMGLWQPMISTLALVIAASLLALIIGIPIGIFSARRQHIWAITRPVLDLMQTMPAFVYLIPAVMFFGTGL
VPSTIATLIFSMPPVVRLTYLGIRQVPVDLIEAGRAFGCSERQLLWKVQLPNALPTLMAGVNQTIMLALSMVVIASMIGG
GGLGDVVLRGIQQLDVGLGFEGGLAVVILAVILDRLTQSLAASNFSRKSLPQRFKAFTNLWTSS

Sequences:

>Translated_304_residues
MMDVIDQVFATVSTGPVGEGMSRFVEWLLNHAQPIFLVIDSAINGLAGAIEQILSVPAPWLLAPLIAILAAWRVSLSFAI
LSLLGLNLVLFMGLWQPMISTLALVIAASLLALIIGIPIGIFSARRQHIWAITRPVLDLMQTMPAFVYLIPAVMFFGTGL
VPSTIATLIFSMPPVVRLTYLGIRQVPVDLIEAGRAFGCSERQLLWKVQLPNALPTLMAGVNQTIMLALSMVVIASMIGG
GGLGDVVLRGIQQLDVGLGFEGGLAVVILAVILDRLTQSLAASNFSRKSLPQRFKAFTNLWTSS
>Mature_304_residues
MMDVIDQVFATVSTGPVGEGMSRFVEWLLNHAQPIFLVIDSAINGLAGAIEQILSVPAPWLLAPLIAILAAWRVSLSFAI
LSLLGLNLVLFMGLWQPMISTLALVIAASLLALIIGIPIGIFSARRQHIWAITRPVLDLMQTMPAFVYLIPAVMFFGTGL
VPSTIATLIFSMPPVVRLTYLGIRQVPVDLIEAGRAFGCSERQLLWKVQLPNALPTLMAGVNQTIMLALSMVVIASMIGG
GGLGDVVLRGIQQLDVGLGFEGGLAVVILAVILDRLTQSLAASNFSRKSLPQRFKAFTNLWTSS

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=276, Percent_Identity=49.6376811594203, Blast_Score=237, Evalue=8e-64,
Organism=Escherichia coli, GI1788451, Length=200, Percent_Identity=34.5, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788449, Length=182, Percent_Identity=32.967032967033, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786564, Length=155, Percent_Identity=30.3225806451613, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32656; Mature: 32656

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDVIDQVFATVSTGPVGEGMSRFVEWLLNHAQPIFLVIDSAINGLAGAIEQILSVPAPW
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LLAPLIAILAAWRVSLSFAILSLLGLNLVLFMGLWQPMISTLALVIAASLLALIIGIPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFSARRQHIWAITRPVLDLMQTMPAFVYLIPAVMFFGTGLVPSTIATLIFSMPPVVRLTY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHH
LGIRQVPVDLIEAGRAFGCSERQLLWKVQLPNALPTLMAGVNQTIMLALSMVVIASMIGG
HHHHHHHHHHHHCCCCCCCCCHHHEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
GGLGDVVLRGIQQLDVGLGFEGGLAVVILAVILDRLTQSLAASNFSRKSLPQRFKAFTNL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
WTSS
HCCC
>Mature Secondary Structure
MMDVIDQVFATVSTGPVGEGMSRFVEWLLNHAQPIFLVIDSAINGLAGAIEQILSVPAPW
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LLAPLIAILAAWRVSLSFAILSLLGLNLVLFMGLWQPMISTLALVIAASLLALIIGIPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFSARRQHIWAITRPVLDLMQTMPAFVYLIPAVMFFGTGLVPSTIATLIFSMPPVVRLTY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHH
LGIRQVPVDLIEAGRAFGCSERQLLWKVQLPNALPTLMAGVNQTIMLALSMVVIASMIGG
HHHHHHHHHHHHCCCCCCCCCHHHEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
GGLGDVVLRGIQQLDVGLGFEGGLAVVILAVILDRLTQSLAASNFSRKSLPQRFKAFTNL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
WTSS
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]