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The map label for this gene is pykF [H]

Identifier: 124023245

GI number: 124023245

Start: 1344316

End: 1346133

Strand: Direct

Name: pykF [H]

Synonym: P9303_15431

Alternate gene names: 124023245

Gene position: 1344316-1346133 (Clockwise)

Preceding gene: 124023242

Following gene: 124023246

Centisome position: 50.11

GC content: 51.87

Gene sequence:

>1818_bases
ATGGTAGTTTCCGCGATGTTTCAGGTCCCGTTGATGGCCCAGATCGATCTGACACGACGGACCAAAATCGTTGCCACCAT
AGGTCCGGCAACCGAGACCGCCGAACGAATCCGAGAACTCGTTCAGGTAGGTGCTACCACCTTCCGGCTGAATTTCTCAC
ACGGCGACCATGACGAGCACGCCAAGCGCATCTCCACCATCCGCCAGGTCTCTGATGAATTGGGGATGCACATTGGCATT
CTTCAAGACTTGCAAGGCCCCAAAATTCGCCTGGGTCGTTTTGATAAGGGGCCTATCACTTTGGCCAATGGCGATCCCTT
CGCACTGACCTCAAAACAAGTTCAATGCAATCAGTCAGTGGCAACCGTCACTTACGAAAAGCTTGCAGACGAAGTCACGA
CCGGCAGTCGAATCTTGCTTGATGACGGTCGAGTGGAGATGAAAGTCGTAAAAATCGATCAAAGCGAACAAACCTTGCAC
TGCATCGTCACAGTGGGAGGGGTGCTCTCAAATAACAAGGGAGTGAATTTCCCTGATGTACAGCTATCGGTTCGCGCCCT
TACAGAGAAAGATCGCCAAGATCTCGCCTTTGGCCTCCAGCAAGGGGTGGACTGGGTCGCACTGAGCTTTGTGCGAAACC
CTTCGGACATGAAAGAGATCCGCAAACTCATCGGGGATCATGGGCACAGCACCCCAGTGATCGCGAAGATCGAGAAATTT
GAGGCGATTGATCAAATCGATTCAATCCTTCCTCTCTGTGATGGAGTCATGGTTGCAAGGGGGGACCTAGGCGTTGAGAT
GCCAGCCGAAGAGGTGCCTCTACTGCAAAAGGATTTGATTCGCAAGGCCAACAGCCTTGGAATTCCAATCATCACAGCCA
CCCAGATGCTCGACTCGATGGCCTCGAGCCCGAGGCCCACCAGAGCAGAAGTGAGTGATGTAGCAAATGCGATCCTTGAT
GGTACTGATGCAGTGATGCTCTCGAACGAAACCGCTGTAGGAGACTATCCAGTGGAGGCGGTTCAAACGATGGCCACCAT
TGCCAGACGGATTGAACGGGACTATCCACAGCGTCCAATCGAAACTTATCTTGCCAGCACCATTTCCAACGCGATTAGTT
CAGCCGTAAGCAGCATCGCCAGTCATCTCAACGCGGCAGCAATCGTGCCACTAACTGAAAGTGGTGCCACTGCCCACAAC
GTCAGCAAATTCCGCCCAGCAGCGCCAATTTTGGCCGTCACCAACGAAGTGGCTGTAGCAAGAAAACTTCAATTGGCATG
GGGTGTCACCCCACTGCTTGTCCCAACCCAGAAAAGCACCACTGCAACCTTCAGTGCTGCAATTGCTGTTGCCCAGGAAT
TGGGTCTTCTCAAAGAAGGAGATCTAACAATTCAAACTGCAGGAACCCTTGCTGGCGTGAGCGGGTCCACAGATCTTGTC
AAAGTAGGAATTGTCAGTGCAGTGCTTGGCCAAGGGATCGGTATTGGCAATGGCACCGTTAGCGGCAAGGTCAGATTTGC
TTTCTCCCCAAACGACTCAACAAAAATTGAACCAGGGGAGATTCTGGTCATTCATGAAACAAGCGATGACTATCTCGATG
CAATCAGAAAGTCTGCAGGAGTGATTACAGAACAAGAAGGAGAGGAATCCCACGCAGCCGTGATCTCAAAACGCCTAGGG
ATTCCGTTCATCATTGGGATAGCACATGCCACGAGAAACCTGCTTGAAGGAGAGGTGGTAACAATGCAGCTCAGTAATGG
CAAAGTTCATCGCGGCACAGGCGGCAAGCTGCCAATGAAGCTCGACTCAATGCTTTGA

Upstream 100 bases:

>100_bases
ATTCGGATAGCTGGCCGTAGCTCGAGCTCCATCCTGGTAATGATTAATGTCCATAGAAAAACATTGGCAACGCTGTCAAC
AACTACGTGTTGAGGCCTGG

Downstream 100 bases:

>100_bases
GGGTTCATGAATAGCTCCCTTCCGCTCATCGAAACGGTGAGCATGGCGTTCTCAACCCTTAAAGCCAATCGCCTACGTAG
TCTCCTGACAATGCTCGGCA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MVVSAMFQVPLMAQIDLTRRTKIVATIGPATETAERIRELVQVGATTFRLNFSHGDHDEHAKRISTIRQVSDELGMHIGI
LQDLQGPKIRLGRFDKGPITLANGDPFALTSKQVQCNQSVATVTYEKLADEVTTGSRILLDDGRVEMKVVKIDQSEQTLH
CIVTVGGVLSNNKGVNFPDVQLSVRALTEKDRQDLAFGLQQGVDWVALSFVRNPSDMKEIRKLIGDHGHSTPVIAKIEKF
EAIDQIDSILPLCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSMASSPRPTRAEVSDVANAILD
GTDAVMLSNETAVGDYPVEAVQTMATIARRIERDYPQRPIETYLASTISNAISSAVSSIASHLNAAAIVPLTESGATAHN
VSKFRPAAPILAVTNEVAVARKLQLAWGVTPLLVPTQKSTTATFSAAIAVAQELGLLKEGDLTIQTAGTLAGVSGSTDLV
KVGIVSAVLGQGIGIGNGTVSGKVRFAFSPNDSTKIEPGEILVIHETSDDYLDAIRKSAGVITEQEGEESHAAVISKRLG
IPFIIGIAHATRNLLEGEVVTMQLSNGKVHRGTGGKLPMKLDSML

Sequences:

>Translated_605_residues
MVVSAMFQVPLMAQIDLTRRTKIVATIGPATETAERIRELVQVGATTFRLNFSHGDHDEHAKRISTIRQVSDELGMHIGI
LQDLQGPKIRLGRFDKGPITLANGDPFALTSKQVQCNQSVATVTYEKLADEVTTGSRILLDDGRVEMKVVKIDQSEQTLH
CIVTVGGVLSNNKGVNFPDVQLSVRALTEKDRQDLAFGLQQGVDWVALSFVRNPSDMKEIRKLIGDHGHSTPVIAKIEKF
EAIDQIDSILPLCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSMASSPRPTRAEVSDVANAILD
GTDAVMLSNETAVGDYPVEAVQTMATIARRIERDYPQRPIETYLASTISNAISSAVSSIASHLNAAAIVPLTESGATAHN
VSKFRPAAPILAVTNEVAVARKLQLAWGVTPLLVPTQKSTTATFSAAIAVAQELGLLKEGDLTIQTAGTLAGVSGSTDLV
KVGIVSAVLGQGIGIGNGTVSGKVRFAFSPNDSTKIEPGEILVIHETSDDYLDAIRKSAGVITEQEGEESHAAVISKRLG
IPFIIGIAHATRNLLEGEVVTMQLSNGKVHRGTGGKLPMKLDSML
>Mature_605_residues
MVVSAMFQVPLMAQIDLTRRTKIVATIGPATETAERIRELVQVGATTFRLNFSHGDHDEHAKRISTIRQVSDELGMHIGI
LQDLQGPKIRLGRFDKGPITLANGDPFALTSKQVQCNQSVATVTYEKLADEVTTGSRILLDDGRVEMKVVKIDQSEQTLH
CIVTVGGVLSNNKGVNFPDVQLSVRALTEKDRQDLAFGLQQGVDWVALSFVRNPSDMKEIRKLIGDHGHSTPVIAKIEKF
EAIDQIDSILPLCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSMASSPRPTRAEVSDVANAILD
GTDAVMLSNETAVGDYPVEAVQTMATIARRIERDYPQRPIETYLASTISNAISSAVSSIASHLNAAAIVPLTESGATAHN
VSKFRPAAPILAVTNEVAVARKLQLAWGVTPLLVPTQKSTTATFSAAIAVAQELGLLKEGDLTIQTAGTLAGVSGSTDLV
KVGIVSAVLGQGIGIGNGTVSGKVRFAFSPNDSTKIEPGEILVIHETSDDYLDAIRKSAGVITEQEGEESHAAVISKRLG
IPFIIGIAHATRNLLEGEVVTMQLSNGKVHRGTGGKLPMKLDSML

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=502, Percent_Identity=39.4422310756972, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI33286420, Length=502, Percent_Identity=39.4422310756972, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI33286418, Length=502, Percent_Identity=39.0438247011952, Blast_Score=327, Evalue=3e-89,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=40, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=40, Blast_Score=325, Evalue=1e-88,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=38.2758620689655, Blast_Score=199, Evalue=7e-51,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=38.2758620689655, Blast_Score=199, Evalue=7e-51,
Organism=Homo sapiens, GI310128736, Length=251, Percent_Identity=33.4661354581673, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI310128734, Length=251, Percent_Identity=33.4661354581673, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI310128738, Length=217, Percent_Identity=33.1797235023041, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=43.7632135306554, Blast_Score=353, Evalue=2e-98,
Organism=Escherichia coli, GI1788160, Length=474, Percent_Identity=40.7172995780591, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1787994, Length=100, Percent_Identity=32, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17544584, Length=436, Percent_Identity=41.0550458715596, Blast_Score=322, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=40.9465020576132, Blast_Score=318, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=40.9465020576132, Blast_Score=318, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=40.9465020576132, Blast_Score=317, Evalue=8e-87,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=40.9465020576132, Blast_Score=317, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6319279, Length=479, Percent_Identity=38.6221294363257, Blast_Score=300, Evalue=3e-82,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=36.9387755102041, Blast_Score=286, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24648964, Length=500, Percent_Identity=38.2, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI28571814, Length=500, Percent_Identity=38.2, Blast_Score=319, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24648966, Length=411, Percent_Identity=41.3625304136253, Blast_Score=298, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24581235, Length=502, Percent_Identity=33.6653386454183, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24646914, Length=269, Percent_Identity=42.3791821561338, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 64593; Mature: 64593

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVSAMFQVPLMAQIDLTRRTKIVATIGPATETAERIRELVQVGATTFRLNFSHGDHDEH
CEEECCCCCCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
AKRISTIRQVSDELGMHIGILQDLQGPKIRLGRFDKGPITLANGDPFALTSKQVQCNQSV
HHHHHHHHHHHHHHCCEEEHHHHCCCCCEEECCCCCCCEEEECCCCCEEECCHHCCCCCH
ATVTYEKLADEVTTGSRILLDDGRVEMKVVKIDQSEQTLHCIVTVGGVLSNNKGVNFPDV
HHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCEEEEEEEECCEEECCCCCCCCCC
QLSVRALTEKDRQDLAFGLQQGVDWVALSFVRNPSDMKEIRKLIGDHGHSTPVIAKIEKF
EEEHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
EAIDQIDSILPLCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSM
HHHHHHHHHHHHHCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHH
ASSPRPTRAEVSDVANAILDGTDAVMLSNETAVGDYPVEAVQTMATIARRIERDYPQRPI
HCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
ETYLASTISNAISSAVSSIASHLNAAAIVPLTESGATAHNVSKFRPAAPILAVTNEVAVA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCCEEEEHHHHHHH
RKLQLAWGVTPLLVPTQKSTTATFSAAIAVAQELGLLKEGDLTIQTAGTLAGVSGSTDLV
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCEECCCEEEEECCCEECCCCCCCHH
KVGIVSAVLGQGIGIGNGTVSGKVRFAFSPNDSTKIEPGEILVIHETSDDYLDAIRKSAG
HHHHHHHHHHCCCCCCCCEECCEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHCC
VITEQEGEESHAAVISKRLGIPFIIGIAHATRNLLEGEVVTMQLSNGKVHRGTGGKLPMK
CCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCH
LDSML
HHCCC
>Mature Secondary Structure
MVVSAMFQVPLMAQIDLTRRTKIVATIGPATETAERIRELVQVGATTFRLNFSHGDHDEH
CEEECCCCCCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
AKRISTIRQVSDELGMHIGILQDLQGPKIRLGRFDKGPITLANGDPFALTSKQVQCNQSV
HHHHHHHHHHHHHHCCEEEHHHHCCCCCEEECCCCCCCEEEECCCCCEEECCHHCCCCCH
ATVTYEKLADEVTTGSRILLDDGRVEMKVVKIDQSEQTLHCIVTVGGVLSNNKGVNFPDV
HHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCEEEEEEEECCEEECCCCCCCCCC
QLSVRALTEKDRQDLAFGLQQGVDWVALSFVRNPSDMKEIRKLIGDHGHSTPVIAKIEKF
EEEHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
EAIDQIDSILPLCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSM
HHHHHHHHHHHHHCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHH
ASSPRPTRAEVSDVANAILDGTDAVMLSNETAVGDYPVEAVQTMATIARRIERDYPQRPI
HCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
ETYLASTISNAISSAVSSIASHLNAAAIVPLTESGATAHNVSKFRPAAPILAVTNEVAVA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCCEEEEHHHHHHH
RKLQLAWGVTPLLVPTQKSTTATFSAAIAVAQELGLLKEGDLTIQTAGTLAGVSGSTDLV
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCEECCCEEEEECCCEECCCCCCCHH
KVGIVSAVLGQGIGIGNGTVSGKVRFAFSPNDSTKIEPGEILVIHETSDDYLDAIRKSAG
HHHHHHHHHHCCCCCCCCEECCEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHCC
VITEQEGEESHAAVISKRLGIPFIIGIAHATRNLLEGEVVTMQLSNGKVHRGTGGKLPMK
CCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCH
LDSML
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]