Click here to switch to the map view.

The map label for this gene is purN [H]

Identifier: 124023213

GI number: 124023213

Start: 1314455

End: 1315207

Strand: Direct

Name: purN [H]

Synonym: P9303_15111

Alternate gene names: 124023213

Gene position: 1314455-1315207 (Clockwise)

Preceding gene: 124023210

Following gene: 124023215

Centisome position: 49.0

GC content: 49.14

Gene sequence:

>753_bases
ATGCAGCTGGTCTTCTCGATCGTCAACAAGATGAACAACTTAGATAAAAAGGAGAATTGTCTGACCAGCGCTTCTTCGCA
TCCTCGTCAGAACAGTTGCTATGAAAAGGATGCGAGGCGATCGCTGATCTGGCCCCCCCCCCAGGCCCGCCAAATATTCA
ACCCTCGCTTAAATCTTGGCGTGATGGCATCAGGGAATGGCAGCAATTTTGAAGCCCTGGTTAAAGCCATTCAAAATTCT
CGACTTGATGCCCATATTTCCATATTGGTGGTTAACAACCCTAATTGTGAAGCACGTCGTCGAGCTCAACGACTAGGTGT
GCCTTGTGTCATCCACAATCATCGTGAATTTAGTAGTCGAGAAGAACTGGATAAGGCCTTGGTAAAGACATTCAGCAACC
ATGCTGTAGAAGGCGTGGTGATGGCCGGCTGGATGCGAATCGTCACCCCTATCCTGATTGCTGCTTTCCCGAATCGCCTT
ATTAATATTCACCCATCTCTGCTACCTAGCTTTCGCGGTTTAGATGCGGTTGGGCAAGCACTAAAAGCGAGAGTTCCTAT
CAGTGGTTGTTCCGTTCACCTGGTTACACCTCAAGTAGATGACGGTCCGGTGCTTGCGCAAGCTGCAGTGCCAGTCTTGA
GCTCAGACGACCATCAGAGCCTCAGCGAAAGGATCCAGCGCATGGAGCATCAACTGCTTCCACTATCTGTTGCTCTTGCA
GGCCAGAATTGGAGAAACGAAGCTCAAAATTAA

Upstream 100 bases:

>100_bases
TGAAGGCTTGACTTTGTCTAAAAAAATCATGGATCGCAGAAGGGGTTCCAATTGGAGGCTGAATACAGGAATTAGGTTGT
GACTTGGATGTGCTTAGAGC

Downstream 100 bases:

>100_bases
GGGTAGAAAGTTAGTAAAGACATCCCTGTGGTTGGTGGCAGTCCAGCCATCAGGTTTAGACATTGAACCCCTTGACCAGC
TTGTCCTTTAATTAGGTTGT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MQLVFSIVNKMNNLDKKENCLTSASSHPRQNSCYEKDARRSLIWPPPQARQIFNPRLNLGVMASGNGSNFEALVKAIQNS
RLDAHISILVVNNPNCEARRRAQRLGVPCVIHNHREFSSREELDKALVKTFSNHAVEGVVMAGWMRIVTPILIAAFPNRL
INIHPSLLPSFRGLDAVGQALKARVPISGCSVHLVTPQVDDGPVLAQAAVPVLSSDDHQSLSERIQRMEHQLLPLSVALA
GQNWRNEAQN

Sequences:

>Translated_250_residues
MQLVFSIVNKMNNLDKKENCLTSASSHPRQNSCYEKDARRSLIWPPPQARQIFNPRLNLGVMASGNGSNFEALVKAIQNS
RLDAHISILVVNNPNCEARRRAQRLGVPCVIHNHREFSSREELDKALVKTFSNHAVEGVVMAGWMRIVTPILIAAFPNRL
INIHPSLLPSFRGLDAVGQALKARVPISGCSVHLVTPQVDDGPVLAQAAVPVLSSDDHQSLSERIQRMEHQLLPLSVALA
GQNWRNEAQN
>Mature_250_residues
MQLVFSIVNKMNNLDKKENCLTSASSHPRQNSCYEKDARRSLIWPPPQARQIFNPRLNLGVMASGNGSNFEALVKAIQNS
RLDAHISILVVNNPNCEARRRAQRLGVPCVIHNHREFSSREELDKALVKTFSNHAVEGVVMAGWMRIVTPILIAAFPNRL
INIHPSLLPSFRGLDAVGQALKARVPISGCSVHLVTPQVDDGPVLAQAAVPVLSSDDHQSLSERIQRMEHQLLPLSVALA
GQNWRNEAQN

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=184, Percent_Identity=35.8695652173913, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI209869995, Length=184, Percent_Identity=35.8695652173913, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI209869993, Length=184, Percent_Identity=35.8695652173913, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1788846, Length=182, Percent_Identity=36.2637362637363, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1787483, Length=146, Percent_Identity=32.8767123287671, Blast_Score=84, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=32.9608938547486, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24582400, Length=186, Percent_Identity=38.1720430107527, Blast_Score=141, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 27676; Mature: 27676

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLVFSIVNKMNNLDKKENCLTSASSHPRQNSCYEKDARRSLIWPPPQARQIFNPRLNLG
CHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHCCCCCCEE
VMASGNGSNFEALVKAIQNSRLDAHISILVVNNPNCEARRRAQRLGVPCVIHNHREFSSR
EEECCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHCCCCEEEECCHHHCHH
EELDKALVKTFSNHAVEGVVMAGWMRIVTPILIAAFPNRLINIHPSLLPSFRGLDAVGQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCHHHCCHHCCHHHHHHH
LKARVPISGCSVHLVTPQVDDGPVLAQAAVPVLSSDDHQSLSERIQRMEHQLLPLSVALA
HHHCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHEEEC
GQNWRNEAQN
CCCCCCCCCC
>Mature Secondary Structure
MQLVFSIVNKMNNLDKKENCLTSASSHPRQNSCYEKDARRSLIWPPPQARQIFNPRLNLG
CHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHCCCCCCEE
VMASGNGSNFEALVKAIQNSRLDAHISILVVNNPNCEARRRAQRLGVPCVIHNHREFSSR
EEECCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHCCCCEEEECCHHHCHH
EELDKALVKTFSNHAVEGVVMAGWMRIVTPILIAAFPNRLINIHPSLLPSFRGLDAVGQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCHHHCCHHCCHHHHHHH
LKARVPISGCSVHLVTPQVDDGPVLAQAAVPVLSSDDHQSLSERIQRMEHQLLPLSVALA
HHHCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHEEEC
GQNWRNEAQN
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]