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The map label for this gene is metG [H]

Identifier: 124023178

GI number: 124023178

Start: 1267972

End: 1269519

Strand: Reverse

Name: metG [H]

Synonym: P9303_14741

Alternate gene names: 124023178

Gene position: 1269519-1267972 (Counterclockwise)

Preceding gene: 124023179

Following gene: 124023177

Centisome position: 47.32

GC content: 44.83

Gene sequence:

>1548_bases
ATGATGACCTATACCATTACGACCCCTCTTTACTACGTCAATGACAAGCCCCATCTAGGCAGCCTGTACACGACCATGGC
CAGTGATGCACTGGCTCGTTTCCAGAGACTAGAAGGTAAGCAGGTCATGTTTGTTACAGGTGTCGATGAACACGGCCAGA
AAATTCAACGCACAGCAGAAGTCAATAACATCAGCCCCCAACAACATTGTGATCAGATCACAAGGAGTTATCAAAATCTA
TGGTCTCGCTGGGATATTTCAAATGATCGCTTTATTCGTACAACCAGCCACCGCCATCAGTTACTGGTCGAGGCTTTTTT
TCAACGTGTAGAGACCTCCGGCGATGTGTATCTTGGTCGGCAGCAGGGTTGGTATTGCGTCGATTGTGAAGAATACAAGG
ACGAACCTCCGCAAGCCCAGTCACCCTCCTGCCCTATTCATCAGAAGCCTCTTGAATGGAGGGATGAGGAGAACCTTTTT
TTCAGACTTTCTCGTTTCCAATCACAAATCGAAGATCTCATTTCACAGCCAGATTTCATCTGGCCAGTCAGTCGCCGGAA
TGAAATACAGAATTTTGTAGCTCAGGGTCTTAGGGATTTTTCAATTTCAAGAATTGATGTTGATTGGGGCTTACCAGTAC
CTGGTTATCCAAAACACACCTTTTATGTCTGGTTTGATGCGCTTGTAGGCTATTTGAGTGCTCTTTTAGATGATGGTGGC
CAGGTTCGAATAGAACGTCTTGTGGAGTGTGGATGGCCTGCCTCAGTACATTTAATTGGCAAGGATATCCTCCGTTTTCA
TGCAGTCTACTGGCCCGCAATGCTGAGCTCAGCAGGCTTACCCTTGCCAAAACAAGTTTTTGGCCACGGCTTTCTCACTC
GTGAAGGTCAAAAGATGGGTAAATCACTAGGCAATGTACTTGATCCAGAGAAACTGATTGAAGTGTGTGGTGAAGATGCT
GTTCGATGGTATTTATTGCGTGACATCCAGTTCGGTCATGATGGTGATTTTCAGCAGAAAAGATTCATGGATTTAGTCAA
TAACGACTTAGCAAATACAATCGGAAATCTGCTGAACCGAACTTCGACTATGGCAAGAAAATGGTTTGAAGGATCTGTCC
CTTCACATCGCAATGAAATAGATTATGCTCATCCCTTAGTGACCACTGCTGAAACAACTATTACCATCGTACGTCAATCA
ATGCCATCCATGGCTTTTCATGATGCAGCTGAGGCGATCCTTAAATTGGCTATTGCAGCCAACGTCTACCTCAATGAGAC
TGCACCTTGGAGTCGAATGAAGACGCCTGGTAATGAAGCCGAAGTGGCTGATGATCTTTACTCAGTTTTGGAGGCATGCC
GACTTGTAGGACTATTAATTAATCCTCTTGTACCTTCTCTAAGCGAACGCTTGCTCAACCAATTAGGACTAAGTATTGAT
AGCAATGACTGGTCTAACAGTTTAGCTTGGGGACAATTGCAAAGTGGAGCTTTATTGCCACCATCTCAACCCGTAATGCA
AAGACTAGAACTCTCTTCTTCCCTCTAA

Upstream 100 bases:

>100_bases
ATGGCTTGGTCTGAGAGATGACGGCAGACGATCAATAAGCTGAAATGAGTTGTTCTGGTTAGGATCCGGCCGCTCTGCGC
AGCAGCAGAAACTCCCCACA

Downstream 100 bases:

>100_bases
TGAGATTCAACCTTCTACCATCACGTCATCTTGGCAGTCTGCTGCTGATCTTAACGATGGTGGGATGTCAGGAATCCCCA
TCAACACAGAAGAAATCATC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MMTYTITTPLYYVNDKPHLGSLYTTMASDALARFQRLEGKQVMFVTGVDEHGQKIQRTAEVNNISPQQHCDQITRSYQNL
WSRWDISNDRFIRTTSHRHQLLVEAFFQRVETSGDVYLGRQQGWYCVDCEEYKDEPPQAQSPSCPIHQKPLEWRDEENLF
FRLSRFQSQIEDLISQPDFIWPVSRRNEIQNFVAQGLRDFSISRIDVDWGLPVPGYPKHTFYVWFDALVGYLSALLDDGG
QVRIERLVECGWPASVHLIGKDILRFHAVYWPAMLSSAGLPLPKQVFGHGFLTREGQKMGKSLGNVLDPEKLIEVCGEDA
VRWYLLRDIQFGHDGDFQQKRFMDLVNNDLANTIGNLLNRTSTMARKWFEGSVPSHRNEIDYAHPLVTTAETTITIVRQS
MPSMAFHDAAEAILKLAIAANVYLNETAPWSRMKTPGNEAEVADDLYSVLEACRLVGLLINPLVPSLSERLLNQLGLSID
SNDWSNSLAWGQLQSGALLPPSQPVMQRLELSSSL

Sequences:

>Translated_515_residues
MMTYTITTPLYYVNDKPHLGSLYTTMASDALARFQRLEGKQVMFVTGVDEHGQKIQRTAEVNNISPQQHCDQITRSYQNL
WSRWDISNDRFIRTTSHRHQLLVEAFFQRVETSGDVYLGRQQGWYCVDCEEYKDEPPQAQSPSCPIHQKPLEWRDEENLF
FRLSRFQSQIEDLISQPDFIWPVSRRNEIQNFVAQGLRDFSISRIDVDWGLPVPGYPKHTFYVWFDALVGYLSALLDDGG
QVRIERLVECGWPASVHLIGKDILRFHAVYWPAMLSSAGLPLPKQVFGHGFLTREGQKMGKSLGNVLDPEKLIEVCGEDA
VRWYLLRDIQFGHDGDFQQKRFMDLVNNDLANTIGNLLNRTSTMARKWFEGSVPSHRNEIDYAHPLVTTAETTITIVRQS
MPSMAFHDAAEAILKLAIAANVYLNETAPWSRMKTPGNEAEVADDLYSVLEACRLVGLLINPLVPSLSERLLNQLGLSID
SNDWSNSLAWGQLQSGALLPPSQPVMQRLELSSSL
>Mature_515_residues
MMTYTITTPLYYVNDKPHLGSLYTTMASDALARFQRLEGKQVMFVTGVDEHGQKIQRTAEVNNISPQQHCDQITRSYQNL
WSRWDISNDRFIRTTSHRHQLLVEAFFQRVETSGDVYLGRQQGWYCVDCEEYKDEPPQAQSPSCPIHQKPLEWRDEENLF
FRLSRFQSQIEDLISQPDFIWPVSRRNEIQNFVAQGLRDFSISRIDVDWGLPVPGYPKHTFYVWFDALVGYLSALLDDGG
QVRIERLVECGWPASVHLIGKDILRFHAVYWPAMLSSAGLPLPKQVFGHGFLTREGQKMGKSLGNVLDPEKLIEVCGEDA
VRWYLLRDIQFGHDGDFQQKRFMDLVNNDLANTIGNLLNRTSTMARKWFEGSVPSHRNEIDYAHPLVTTAETTITIVRQS
MPSMAFHDAAEAILKLAIAANVYLNETAPWSRMKTPGNEAEVADDLYSVLEACRLVGLLINPLVPSLSERLLNQLGLSID
SNDWSNSLAWGQLQSGALLPPSQPVMQRLELSSSL

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=504, Percent_Identity=35.7142857142857, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI14043022, Length=548, Percent_Identity=25, Blast_Score=152, Evalue=1e-36,
Organism=Escherichia coli, GI1788432, Length=501, Percent_Identity=27.3453093812375, Blast_Score=192, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI212645096, Length=512, Percent_Identity=31.4453125, Blast_Score=261, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI71992781, Length=407, Percent_Identity=30.2211302211302, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17540794, Length=525, Percent_Identity=25.1428571428571, Blast_Score=147, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321610, Length=524, Percent_Identity=33.0152671755725, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6321704, Length=530, Percent_Identity=23.5849056603774, Blast_Score=126, Evalue=9e-30,
Organism=Drosophila melanogaster, GI21358483, Length=505, Percent_Identity=32.8712871287129, Blast_Score=282, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24655311, Length=569, Percent_Identity=24.4288224956063, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58679; Mature: 58679

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTYTITTPLYYVNDKPHLGSLYTTMASDALARFQRLEGKQVMFVTGVDEHGQKIQRTAE
CEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
VNNISPQQHCDQITRSYQNLWSRWDISNDRFIRTTSHRHQLLVEAFFQRVETSGDVYLGR
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEC
QQGWYCVDCEEYKDEPPQAQSPSCPIHQKPLEWRDEENLFFRLSRFQSQIEDLISQPDFI
CCCEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
WPVSRRNEIQNFVAQGLRDFSISRIDVDWGLPVPGYPKHTFYVWFDALVGYLSALLDDGG
ECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
QVRIERLVECGWPASVHLIGKDILRFHAVYWPAMLSSAGLPLPKQVFGHGFLTREGQKMG
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHH
KSLGNVLDPEKLIEVCGEDAVRWYLLRDIQFGHDGDFQQKRFMDLVNNDLANTIGNLLNR
HHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TSTMARKWFEGSVPSHRNEIDYAHPLVTTAETTITIVRQSMPSMAFHDAAEAILKLAIAA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
NVYLNETAPWSRMKTPGNEAEVADDLYSVLEACRLVGLLINPLVPSLSERLLNQLGLSID
HEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SNDWSNSLAWGQLQSGALLPPSQPVMQRLELSSSL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MMTYTITTPLYYVNDKPHLGSLYTTMASDALARFQRLEGKQVMFVTGVDEHGQKIQRTAE
CEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
VNNISPQQHCDQITRSYQNLWSRWDISNDRFIRTTSHRHQLLVEAFFQRVETSGDVYLGR
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEC
QQGWYCVDCEEYKDEPPQAQSPSCPIHQKPLEWRDEENLFFRLSRFQSQIEDLISQPDFI
CCCEEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
WPVSRRNEIQNFVAQGLRDFSISRIDVDWGLPVPGYPKHTFYVWFDALVGYLSALLDDGG
ECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
QVRIERLVECGWPASVHLIGKDILRFHAVYWPAMLSSAGLPLPKQVFGHGFLTREGQKMG
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHH
KSLGNVLDPEKLIEVCGEDAVRWYLLRDIQFGHDGDFQQKRFMDLVNNDLANTIGNLLNR
HHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TSTMARKWFEGSVPSHRNEIDYAHPLVTTAETTITIVRQSMPSMAFHDAAEAILKLAIAA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
NVYLNETAPWSRMKTPGNEAEVADDLYSVLEACRLVGLLINPLVPSLSERLLNQLGLSID
HEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SNDWSNSLAWGQLQSGALLPPSQPVMQRLELSSSL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]