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The map label for this gene is tpiA

Identifier: 124023096

GI number: 124023096

Start: 1186940

End: 1187671

Strand: Direct

Name: tpiA

Synonym: P9303_13911

Alternate gene names: 124023096

Gene position: 1186940-1187671 (Clockwise)

Preceding gene: 124023095

Following gene: 124023097

Centisome position: 44.24

GC content: 53.28

Gene sequence:

>732_bases
GTGCGTAAACCCGTGATCGCTGGCAACTGGAAGATGCACATGACCTGTGCTCAGGCACGGGAGTACACGGCCATGTTCCT
GCCATTAATCGAAGCCACTCCCAATGACCGTCATGTAGTGATTGCCCCACCATTCACCGCCATCTCTTCAATGGCAGAGG
CCGTTGGCGGCACTCGTCTGGAATTGTCAAGCCAGAATGTGCATTGGGAGGATGATGGTGCCTATACCGCAGAGGTTTCG
CCAACGATGCTTCTGGAGCATCAAGTGCGATACGCGATTGTTGGCCATAGTGAACCACGTAAATACTTCAGTGAGAGCGA
TGAACAGATCAATCGTCGTGCTCGTTCAGCCCAAGCGCATGGTCTGATCCCGATTGTCTGCGTGGGTGAAAGTGATGAGC
AGCGTGAACGCGGAGAGGCTGAGCGTGTGATTCGACGACAGGTGGAACAGGGACTGGAAGACACCGATCCAGACAAACTT
GTGGTTGCTTATGAGCCCATCTGGGCCATTGGCACGGGCAAGACGTGCGAAGCCTCGGAGGCGAATCGTATCTGTGGCCT
CATTCGTGGTTGGGTCGGTGCTTCAGAACTAATCATTCAGTACGGCGGTTCGGTCAAACCAGGAAATATCGATGAACTGA
TGGCGATGAGTGATATCGACGGAGTGTTGGTGGGTGGTGCTTCCCTTGACCCAGAGAGTTTTGCTCGCATTGCCAATTAT
CAAACCAGTTAA

Upstream 100 bases:

>100_bases
AGGCAATCGGGTTCGATTGGGCACAGATGAGGCGGTCTTTGTCGGCAATGCTTCGACAGGGCCGTAAGTTAGCCCCTGCG
GAACTTCAGAGGACCCGAGC

Downstream 100 bases:

>100_bases
ATCTCCTTGGCCTCAGGGTTGGGGACAGCGAACGAATGTGATGGGGGTGATTAATATCACTCCTGATTCATTTAGCGATG
GTGGCAATTATCTATATCCT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRKPVIAGNWKMHMTCAQAREYTAMFLPLIEATPNDRHVVIAPPFTAISSMAEAVGGTRLELSSQNVHWEDDGAYTAEVS
PTMLLEHQVRYAIVGHSEPRKYFSESDEQINRRARSAQAHGLIPIVCVGESDEQRERGEAERVIRRQVEQGLEDTDPDKL
VVAYEPIWAIGTGKTCEASEANRICGLIRGWVGASELIIQYGGSVKPGNIDELMAMSDIDGVLVGGASLDPESFARIANY
QTS

Sequences:

>Translated_243_residues
MRKPVIAGNWKMHMTCAQAREYTAMFLPLIEATPNDRHVVIAPPFTAISSMAEAVGGTRLELSSQNVHWEDDGAYTAEVS
PTMLLEHQVRYAIVGHSEPRKYFSESDEQINRRARSAQAHGLIPIVCVGESDEQRERGEAERVIRRQVEQGLEDTDPDKL
VVAYEPIWAIGTGKTCEASEANRICGLIRGWVGASELIIQYGGSVKPGNIDELMAMSDIDGVLVGGASLDPESFARIANY
QTS
>Mature_243_residues
MRKPVIAGNWKMHMTCAQAREYTAMFLPLIEATPNDRHVVIAPPFTAISSMAEAVGGTRLELSSQNVHWEDDGAYTAEVS
PTMLLEHQVRYAIVGHSEPRKYFSESDEQINRRARSAQAHGLIPIVCVGESDEQRERGEAERVIRRQVEQGLEDTDPDKL
VVAYEPIWAIGTGKTCEASEANRICGLIRGWVGASELIIQYGGSVKPGNIDELMAMSDIDGVLVGGASLDPESFARIANY
QTS

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=247, Percent_Identity=38.8663967611336, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI226529917, Length=248, Percent_Identity=38.3064516129032, Blast_Score=152, Evalue=2e-37,
Organism=Escherichia coli, GI1790353, Length=246, Percent_Identity=38.2113821138211, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17536593, Length=249, Percent_Identity=36.144578313253, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=34.9593495934959, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI28572008, Length=246, Percent_Identity=39.8373983739837, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI28572006, Length=246, Percent_Identity=39.8373983739837, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=40.8906882591093, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_PROM3 (A2C9H8)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001017403.1
- ProteinModelPortal:   A2C9H8
- SMR:   A2C9H8
- STRING:   A2C9H8
- GeneID:   4777038
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_13911
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26715; Mature: 26715

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 96-96 ACT_SITE 165-165 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPVIAGNWKMHMTCAQAREYTAMFLPLIEATPNDRHVVIAPPFTAISSMAEAVGGTRL
CCCCEEECCCEEEEEHHHHHHHHHHEEEHHCCCCCCCEEEEECCHHHHHHHHHHHCCCEE
ELSSQNVHWEDDGAYTAEVSPTMLLEHQVRYAIVGHSEPRKYFSESDEQINRRARSAQAH
EECCCCCEECCCCCEEEECCCHHHHHHCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHCC
GLIPIVCVGESDEQRERGEAERVIRRQVEQGLEDTDPDKLVVAYEPIWAIGTGKTCEASE
CCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCCCCH
ANRICGLIRGWVGASELIIQYGGSVKPGNIDELMAMSDIDGVLVGGASLDPESFARIANY
HHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCC
QTS
CCC
>Mature Secondary Structure
MRKPVIAGNWKMHMTCAQAREYTAMFLPLIEATPNDRHVVIAPPFTAISSMAEAVGGTRL
CCCCEEECCCEEEEEHHHHHHHHHHEEEHHCCCCCCCEEEEECCHHHHHHHHHHHCCCEE
ELSSQNVHWEDDGAYTAEVSPTMLLEHQVRYAIVGHSEPRKYFSESDEQINRRARSAQAH
EECCCCCEECCCCCEEEECCCHHHHHHCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHCC
GLIPIVCVGESDEQRERGEAERVIRRQVEQGLEDTDPDKLVVAYEPIWAIGTGKTCEASE
CCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEECCCCCCCCCH
ANRICGLIRGWVGASELIIQYGGSVKPGNIDELMAMSDIDGVLVGGASLDPESFARIANY
HHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCC
QTS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA