Click here to switch to the map view.

The map label for this gene is mscL [H]

Identifier: 124023048

GI number: 124023048

Start: 1148488

End: 1148880

Strand: Direct

Name: mscL [H]

Synonym: P9303_13431

Alternate gene names: 124023048

Gene position: 1148488-1148880 (Clockwise)

Preceding gene: 124023043

Following gene: 124023049

Centisome position: 42.81

GC content: 49.36

Gene sequence:

>393_bases
ATGGCATACCGTCGCAAAATAACCAACGTCCTTAATGACTTCACTGACTTTGTCAATCGCGGCAATGTCGTAGATCTCGC
GGTCGCTGTTGTCGTAGGTGGTGCCTTCTCCAAGGTGGTGGATGCACTTGTTAAGTTGGTCACTACCGCTGTGATGGATC
CTGTACTTCGTAAACTTAAGGTAGAGACACTTAGTGCCGTGCCCGGCGGCGGACTTTTGGTCTCAATGATTAATTTTTTG
GTCATTGCCTTCGTGGTATTTATTGTGGTGAGGGCTATCGAGCGCTTCAAACGCAAAGAGGAGGCTAAAGCTGCTGCAGC
TCCAAACCCCCAGCAGGATTTAGCTGATGCTGTTGAGCGTTTAGCAATTGCGCTCGAATCCCGTAAGCTCTGA

Upstream 100 bases:

>100_bases
CCCTGATGATTTGGAAGGGGCAAATCGGCTGAATTTTTGAGGATTGTGGCGTCATCTCACTGAGAGAGTCATTATGGTGT
CTTTTTAGTCATTGCTTCAT

Downstream 100 bases:

>100_bases
GATGATCACAGTATTAACCAACAGCCTTTATCTTGTCAATATTCTTACTTACTAGTACTTATCCTGTTCCTAATTATCCA
CTAGACTCTTAAGAACTCCA

Product: large-conductance mechanosensitive channel mscL

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MAYRRKITNVLNDFTDFVNRGNVVDLAVAVVVGGAFSKVVDALVKLVTTAVMDPVLRKLKVETLSAVPGGGLLVSMINFL
VIAFVVFIVVRAIERFKRKEEAKAAAAPNPQQDLADAVERLAIALESRKL

Sequences:

>Translated_130_residues
MAYRRKITNVLNDFTDFVNRGNVVDLAVAVVVGGAFSKVVDALVKLVTTAVMDPVLRKLKVETLSAVPGGGLLVSMINFL
VIAFVVFIVVRAIERFKRKEEAKAAAAPNPQQDLADAVERLAIALESRKL
>Mature_129_residues
AYRRKITNVLNDFTDFVNRGNVVDLAVAVVVGGAFSKVVDALVKLVTTAVMDPVLRKLKVETLSAVPGGGLLVSMINFLV
IAFVVFIVVRAIERFKRKEEAKAAAAPNPQQDLADAVERLAIALESRKL

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=121, Percent_Identity=32.2314049586777, Blast_Score=63, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14093; Mature: 13962

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYRRKITNVLNDFTDFVNRGNVVDLAVAVVVGGAFSKVVDALVKLVTTAVMDPVLRKLK
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VETLSAVPGGGLLVSMINFLVIAFVVFIVVRAIERFKRKEEAKAAAAPNPQQDLADAVER
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LAIALESRKL
HHHHHHHCCC
>Mature Secondary Structure 
AYRRKITNVLNDFTDFVNRGNVVDLAVAVVVGGAFSKVVDALVKLVTTAVMDPVLRKLK
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VETLSAVPGGGLLVSMINFLVIAFVVFIVVRAIERFKRKEEAKAAAAPNPQQDLADAVER
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LAIALESRKL
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]