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The map label for this gene is phoR [H]

Identifier: 124022817

GI number: 124022817

Start: 993704

End: 994789

Strand: Reverse

Name: phoR [H]

Synonym: P9303_11101

Alternate gene names: 124022817

Gene position: 994789-993704 (Counterclockwise)

Preceding gene: 124022818

Following gene: 124022815

Centisome position: 37.08

GC content: 39.23

Gene sequence:

>1086_bases
TTGTTAAAGAGATCACCTAAAGAAAAAATAGATCCACAGATCAATGACTTTCTTAGCTTAAACGGGATTAGCATCAATAC
TCCTCAGTTATTGGCCTGGATTGACGTTGCCAGTCAAGGGTGGATAATTCTAACTCCCGCATTTGATATTGCTTATATCA
ATATCAAGGCACAACGTCTATTGCAGATTACTGGAAAAAAGCTTGTTCAAGGCCATTCATTATCTCAAATAATCAAATCT
ACGTTATTAAATGATGCGATTCGAGAGGTTCGACAACAACAGCTTCCTCAAAGAGTTGAATTTGATGTTAACAACATACC
TCTAGAGGCCTATGTTCATCCAGGTATAGAAGAATGCCTTTTAGTTTTGCTTTCAAGTCGCCGATCAATAGAGGCGCAAC
AACAACAACAAGAACGTTGGTTGAGTGATGTAGCACATGAGCTTAAAACTCCTCTAACAGCCTTAATTTTGGTTAGTGAT
CGGTTAGAGCAGATAATCCCGGGCAAAGATTCAGTGTTGGTAGATCGTATGCAAAGAGAACTACAAAGACTTCAAAAAAT
GCTTGAAGATCTTTTTGAACTTTCAAGGCTTGAGAATAAACAGCCTATAGATAATACAAATTACATTGCTAATTCTATAG
AGGATCTCCTGGAAAATGCCTGGAATACCATTCGCCCTATAGCTGAACTACGTAATGTCAACCTCATAACATATACTGAC
GAGCCTGGACTGATACTTGGTGATAAGCAACGACTACAAAGAGCATTGATTAATTTGCTGGATAACGCTCTGCGTTACTC
ACCTGAGAATGGCTCTATTGAAGTAGATATCCTTCCAAAGGGACTTTGGTGGCTTATCAAGATCCGTGATCATGGCCCTG
GACTTAGTGAGATTGACTTAAGCAATATGTTTCAGCGTTTTTATCGAGGAGATATCTCAAGAAAACGAACAAGCCGAAGT
GGCAGTGGTCTTGGATTAGCCATTGTCGAGCAAATTATTATCAGTCATGGCGGAAGCATCAAAGCAGAAAATCACCCTGA
AGGAGGAACTAGTATCGAACTTTTACTACCTAAAGGCAAATACTAA

Upstream 100 bases:

>100_bases
CTGATTGGAATCAACTCAATTGCCCTGCAGGCAAGATCACTTGGGTGCATCGCTCTGATGGCTCTGGCACCACCAAGGCT
TTTACCAAACAAGGCAAAAC

Downstream 100 bases:

>100_bases
GCAATAATCGAAAAATAAAATAAAATTGTCGTAGTCAAGATGCTTTGAGAATATGCTATTTGAAAATAAAAAAATGCCCT
CCGTTTAAGGAGGGCATTGA

Product: two-component sensor histidine kinase, phosphate sensing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MLKRSPKEKIDPQINDFLSLNGISINTPQLLAWIDVASQGWIILTPAFDIAYINIKAQRLLQITGKKLVQGHSLSQIIKS
TLLNDAIREVRQQQLPQRVEFDVNNIPLEAYVHPGIEECLLVLLSSRRSIEAQQQQQERWLSDVAHELKTPLTALILVSD
RLEQIIPGKDSVLVDRMQRELQRLQKMLEDLFELSRLENKQPIDNTNYIANSIEDLLENAWNTIRPIAELRNVNLITYTD
EPGLILGDKQRLQRALINLLDNALRYSPENGSIEVDILPKGLWWLIKIRDHGPGLSEIDLSNMFQRFYRGDISRKRTSRS
GSGLGLAIVEQIIISHGGSIKAENHPEGGTSIELLLPKGKY

Sequences:

>Translated_361_residues
MLKRSPKEKIDPQINDFLSLNGISINTPQLLAWIDVASQGWIILTPAFDIAYINIKAQRLLQITGKKLVQGHSLSQIIKS
TLLNDAIREVRQQQLPQRVEFDVNNIPLEAYVHPGIEECLLVLLSSRRSIEAQQQQQERWLSDVAHELKTPLTALILVSD
RLEQIIPGKDSVLVDRMQRELQRLQKMLEDLFELSRLENKQPIDNTNYIANSIEDLLENAWNTIRPIAELRNVNLITYTD
EPGLILGDKQRLQRALINLLDNALRYSPENGSIEVDILPKGLWWLIKIRDHGPGLSEIDLSNMFQRFYRGDISRKRTSRS
GSGLGLAIVEQIIISHGGSIKAENHPEGGTSIELLLPKGKY
>Mature_361_residues
MLKRSPKEKIDPQINDFLSLNGISINTPQLLAWIDVASQGWIILTPAFDIAYINIKAQRLLQITGKKLVQGHSLSQIIKS
TLLNDAIREVRQQQLPQRVEFDVNNIPLEAYVHPGIEECLLVLLSSRRSIEAQQQQQERWLSDVAHELKTPLTALILVSD
RLEQIIPGKDSVLVDRMQRELQRLQKMLEDLFELSRLENKQPIDNTNYIANSIEDLLENAWNTIRPIAELRNVNLITYTD
EPGLILGDKQRLQRALINLLDNALRYSPENGSIEVDILPKGLWWLIKIRDHGPGLSEIDLSNMFQRFYRGDISRKRTSRS
GSGLGLAIVEQIIISHGGSIKAENHPEGGTSIELLLPKGKY

Specific function: Member of the two-component regulatory system sphR/sphS. Sensory kinase. Is involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to phos

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790346, Length=276, Percent_Identity=35.1449275362319, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788393, Length=239, Percent_Identity=33.0543933054393, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786783, Length=262, Percent_Identity=31.2977099236641, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI145693157, Length=313, Percent_Identity=28.7539936102236, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786912, Length=238, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789149, Length=239, Percent_Identity=28.4518828451883, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790861, Length=228, Percent_Identity=29.8245614035088, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789403, Length=223, Percent_Identity=30.4932735426009, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787894, Length=232, Percent_Identity=28.448275862069, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786600, Length=256, Percent_Identity=26.953125, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081816, Length=246, Percent_Identity=27.6422764227642, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789808, Length=222, Percent_Identity=28.8288288288288, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790436, Length=245, Percent_Identity=26.530612244898, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788279, Length=205, Percent_Identity=29.2682926829268, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790551, Length=226, Percent_Identity=28.7610619469027, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788549, Length=255, Percent_Identity=28.2352941176471, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=23.9130434782609, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48994928, Length=236, Percent_Identity=22.0338983050847, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41016; Mature: 41016

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRSPKEKIDPQINDFLSLNGISINTPQLLAWIDVASQGWIILTPAFDIAYINIKAQRL
CCCCCCHHHCCCCHHHHEEECCCEECCCHHEEEEEECCCCEEEEECCCEEEEEEHHHHHH
LQITGKKLVQGHSLSQIIKSTLLNDAIREVRQQQLPQRVEFDVNNIPLEAYVHPGIEECL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEECCCHHHHH
LVLLSSRRSIEAQQQQQERWLSDVAHELKTPLTALILVSDRLEQIIPGKDSVLVDRMQRE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LQRLQKMLEDLFELSRLENKQPIDNTNYIANSIEDLLENAWNTIRPIAELRNVNLITYTD
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
EPGLILGDKQRLQRALINLLDNALRYSPENGSIEVDILPKGLWWLIKIRDHGPGLSEIDL
CCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCH
SNMFQRFYRGDISRKRTSRSGSGLGLAIVEQIIISHGGSIKAENHPEGGTSIELLLPKGK
HHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCC
Y
C
>Mature Secondary Structure
MLKRSPKEKIDPQINDFLSLNGISINTPQLLAWIDVASQGWIILTPAFDIAYINIKAQRL
CCCCCCHHHCCCCHHHHEEECCCEECCCHHEEEEEECCCCEEEEECCCEEEEEEHHHHHH
LQITGKKLVQGHSLSQIIKSTLLNDAIREVRQQQLPQRVEFDVNNIPLEAYVHPGIEECL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEECCCHHHHH
LVLLSSRRSIEAQQQQQERWLSDVAHELKTPLTALILVSDRLEQIIPGKDSVLVDRMQRE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LQRLQKMLEDLFELSRLENKQPIDNTNYIANSIEDLLENAWNTIRPIAELRNVNLITYTD
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
EPGLILGDKQRLQRALINLLDNALRYSPENGSIEVDILPKGLWWLIKIRDHGPGLSEIDL
CCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCH
SNMFQRFYRGDISRKRTSRSGSGLGLAIVEQIIISHGGSIKAENHPEGGTSIELLLPKGK
HHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497200 [H]