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The map label for this gene is proP [H]

Identifier: 124022724

GI number: 124022724

Start: 926715

End: 927977

Strand: Direct

Name: proP [H]

Synonym: P9303_10171

Alternate gene names: 124022724

Gene position: 926715-927977 (Clockwise)

Preceding gene: 124022721

Following gene: 124022729

Centisome position: 34.54

GC content: 45.37

Gene sequence:

>1263_bases
ATGATCGTAAAGGCCCAAGAGACATCAAACATACGTGTGATACTCGCTGGTCTCGTTGGCAACGTGATCGAATGGTATGA
CTTCGCTTTGTATGGATACTTTGCCAGCGTTATTGGAAAACAGTTCTTTCCCTCTAGTAATCCTTCAGTCTCTCTAATTG
CTGCTTTCGGAGCGTTTGCTGTCGGCTTTCTAGTTCGCCCTTTCGGAGGACTTTTGTTCGGACGTATTGCTGATTTGCTG
GGACGAAAACAGGCGCTTATCCTTACCTTGCTGGCGATGGCTATCCCAACAGTGCTGATGGCCTGTATGCCCAACTACAG
CAGGATTGGCATAGCTGCTCCGATCATAATCGTTTTGTTGCGTATTATCCAAGGATTATCAGTTGGCGGCGAGTACACAA
CATCAATTGTTTATCTCGTTGAGAATGCCCCTGATAAACGACGAGCCTTCTTTGCTATTTGGGGTCTATGGGGAGCAGTA
TTGGGAATCCTCTTGGCTTCTGCCATAGCCAGTTTGCTTGCCAATATTCTTGACCCTCAACAGCTAGACATCTGGGGTTG
GAGAGTGCCTTTTGCGCTCGGTTCACTTGTCGCATTAATAGGACTTTTAATACGACGTGGTCTTGTAACTGATGTATGTA
CTGAAGAGGCAATAGACCCAGTACAGCAGGTTTTCGGCAAATACCGTATGCAGGTATTACGCTTGTTCTTGCTTAATATT
GGTGGCGGTGTTGGCTTCTATGCAGCTTTTGTGTATGTTGTGAGTTACGTCAAGGAAATAGATATGGTGCCCGAACGAAT
AGCTCTGAATATAAATACAGTTTCTATGGCAATACTTTTAATACTTTATCCATTAACCGCTTGGCTTTCAGACCGCATTG
GACGTAAGCCCTTGTTGATCGCTGGTGGTGGCATGTTGATGTTTGGCTCGATTCCACTTTTTCACTTGATTCACACCACT
GATCCATTACGAATTTTCTTTGGGCAGCTCGGATTTGTGATTGCACTCGCAACTCTTTCAGGAGGATTAAATGTCGCGAA
TGTGGAGCTTATGCCTAAGGCGGTTCGCTGTACCGGCCTGGCCTTTGCCTACAACACTTCTATGGGGATTTTTGGTGGTA
CAACACCATTAATTGCGACCTGGTTAATTCAGGGAAGTGGAAATCCAATTAGCCCTGCTTACTGGTTAGCGGGCAGTGCT
TCGATCACTTTATTAACAAGTATCTTTTGGGTTAGAGAAACGAGACTTTCAAGCCTGTCTTGA

Upstream 100 bases:

>100_bases
CTCTCAATTGCACCATTTATGATTGAGTCTTAGATCAGAATTACTTGCTTAATAATGACTAGAGATTCACCTTGACACAG
CATTACTTTGGTTCTTTGGC

Downstream 100 bases:

>100_bases
ACATAGTAAATGAAATAAAGCACCTCTATTCCCCAATTAAGGTTATTTACTAGCCCATCATTAAGCTGAGGCGATACCCT
CAAATTGTTTGGTTTGTGTT

Product: proline/betaine transporter MFS family protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MIVKAQETSNIRVILAGLVGNVIEWYDFALYGYFASVIGKQFFPSSNPSVSLIAAFGAFAVGFLVRPFGGLLFGRIADLL
GRKQALILTLLAMAIPTVLMACMPNYSRIGIAAPIIIVLLRIIQGLSVGGEYTTSIVYLVENAPDKRRAFFAIWGLWGAV
LGILLASAIASLLANILDPQQLDIWGWRVPFALGSLVALIGLLIRRGLVTDVCTEEAIDPVQQVFGKYRMQVLRLFLLNI
GGGVGFYAAFVYVVSYVKEIDMVPERIALNINTVSMAILLILYPLTAWLSDRIGRKPLLIAGGGMLMFGSIPLFHLIHTT
DPLRIFFGQLGFVIALATLSGGLNVANVELMPKAVRCTGLAFAYNTSMGIFGGTTPLIATWLIQGSGNPISPAYWLAGSA
SITLLTSIFWVRETRLSSLS

Sequences:

>Translated_420_residues
MIVKAQETSNIRVILAGLVGNVIEWYDFALYGYFASVIGKQFFPSSNPSVSLIAAFGAFAVGFLVRPFGGLLFGRIADLL
GRKQALILTLLAMAIPTVLMACMPNYSRIGIAAPIIIVLLRIIQGLSVGGEYTTSIVYLVENAPDKRRAFFAIWGLWGAV
LGILLASAIASLLANILDPQQLDIWGWRVPFALGSLVALIGLLIRRGLVTDVCTEEAIDPVQQVFGKYRMQVLRLFLLNI
GGGVGFYAAFVYVVSYVKEIDMVPERIALNINTVSMAILLILYPLTAWLSDRIGRKPLLIAGGGMLMFGSIPLFHLIHTT
DPLRIFFGQLGFVIALATLSGGLNVANVELMPKAVRCTGLAFAYNTSMGIFGGTTPLIATWLIQGSGNPISPAYWLAGSA
SITLLTSIFWVRETRLSSLS
>Mature_420_residues
MIVKAQETSNIRVILAGLVGNVIEWYDFALYGYFASVIGKQFFPSSNPSVSLIAAFGAFAVGFLVRPFGGLLFGRIADLL
GRKQALILTLLAMAIPTVLMACMPNYSRIGIAAPIIIVLLRIIQGLSVGGEYTTSIVYLVENAPDKRRAFFAIWGLWGAV
LGILLASAIASLLANILDPQQLDIWGWRVPFALGSLVALIGLLIRRGLVTDVCTEEAIDPVQQVFGKYRMQVLRLFLLNI
GGGVGFYAAFVYVVSYVKEIDMVPERIALNINTVSMAILLILYPLTAWLSDRIGRKPLLIAGGGMLMFGSIPLFHLIHTT
DPLRIFFGQLGFVIALATLSGGLNVANVELMPKAVRCTGLAFAYNTSMGIFGGTTPLIATWLIQGSGNPISPAYWLAGSA
SITLLTSIFWVRETRLSSLS

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=419, Percent_Identity=33.4128878281623, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1788292, Length=420, Percent_Identity=31.6666666666667, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788942, Length=384, Percent_Identity=31.7708333333333, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789941, Length=427, Percent_Identity=30.2107728337237, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI87082231, Length=216, Percent_Identity=25.462962962963, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082404, Length=386, Percent_Identity=23.8341968911917, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45412; Mature: 45412

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVKAQETSNIRVILAGLVGNVIEWYDFALYGYFASVIGKQFFPSSNPSVSLIAAFGAFA
CEEECCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VGFLVRPFGGLLFGRIADLLGRKQALILTLLAMAIPTVLMACMPNYSRIGIAAPIIIVLL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RIIQGLSVGGEYTTSIVYLVENAPDKRRAFFAIWGLWGAVLGILLASAIASLLANILDPQ
HHHHCCCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QLDIWGWRVPFALGSLVALIGLLIRRGLVTDVCTEEAIDPVQQVFGKYRMQVLRLFLLNI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGGVGFYAAFVYVVSYVKEIDMVPERIALNINTVSMAILLILYPLTAWLSDRIGRKPLLI
CCCHHHHHHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
AGGGMLMFGSIPLFHLIHTTDPLRIFFGQLGFVIALATLSGGLNVANVELMPKAVRCTGL
ECCCEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHE
AFAYNTSMGIFGGTTPLIATWLIQGSGNPISPAYWLAGSASITLLTSIFWVRETRLSSLS
EEEEECCCCCCCCCHHHHHHHHEECCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIVKAQETSNIRVILAGLVGNVIEWYDFALYGYFASVIGKQFFPSSNPSVSLIAAFGAFA
CEEECCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VGFLVRPFGGLLFGRIADLLGRKQALILTLLAMAIPTVLMACMPNYSRIGIAAPIIIVLL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RIIQGLSVGGEYTTSIVYLVENAPDKRRAFFAIWGLWGAVLGILLASAIASLLANILDPQ
HHHHCCCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QLDIWGWRVPFALGSLVALIGLLIRRGLVTDVCTEEAIDPVQQVFGKYRMQVLRLFLLNI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGGVGFYAAFVYVVSYVKEIDMVPERIALNINTVSMAILLILYPLTAWLSDRIGRKPLLI
CCCHHHHHHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
AGGGMLMFGSIPLFHLIHTTDPLRIFFGQLGFVIALATLSGGLNVANVELMPKAVRCTGL
ECCCEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHE
AFAYNTSMGIFGGTTPLIATWLIQGSGNPISPAYWLAGSASITLLTSIFWVRETRLSSLS
EEEEECCCCCCCCCHHHHHHHHEECCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]