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The map label for this gene is ruvC

Identifier: 124022603

GI number: 124022603

Start: 810228

End: 810692

Strand: Reverse

Name: ruvC

Synonym: P9303_08941

Alternate gene names: 124022603

Gene position: 810692-810228 (Counterclockwise)

Preceding gene: 124022604

Following gene: 124022602

Centisome position: 30.22

GC content: 57.85

Gene sequence:

>465_bases
TTGCGCATCCTTGGCATCGACCCTGGCCTCGCCAGGGTGGGCTATGGGGTCATTGACACCAGCAACGGACAACAGCAGAT
GCTCGATTGCGGCATCATCCGCACCAATCCCGGCATTGACGATGGTGTACGGATGGTGGAAATCGCCAGCGATCTACGCC
AGCTCATTCGTCGTTGGAAGCCCCAACTAGCCGCTGTGGAGAAATTCTTCTTCTACCGCTCCAGCACGACGATCAGCGTG
GTTCAGGCACGTGGAGTCATCATCATGACCCTGGCACGCTTTCGAGTGCCAATGGTGGAATTCCCACCGATGCAAATCAA
GCTTGCTCTGGCGGGCTCAGGCCATGCTGAAAAAGATGAAGTGCTCGATGCGGTGATGCGCGAATTGAATCTTGATCAGC
CGCCCCGACCCGACGATGCCGCCGACGCCCTGGCCATAGCACTCACGGGTTGGTTCCAGCGCTAA

Upstream 100 bases:

>100_bases
TCTGGGGATCGCGTCGTCAAAATTTTCTGCAAAGTGTTCGAACGCAGCGAAAACAGCGATCAGGCTGACTTCGAGCTAAC
AACTCAGGCAGGCTGAGCGC

Downstream 100 bases:

>100_bases
AACCACTCATGGACTCCTCCTGCTCACCAAAACATGGCTGGTTTGACAGCGTGGCTGCGTCTTACGCCCAACAACGCCCC
CATTACCCAGAATCCCTGTT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MRILGIDPGLARVGYGVIDTSNGQQQMLDCGIIRTNPGIDDGVRMVEIASDLRQLIRRWKPQLAAVEKFFFYRSSTTISV
VQARGVIIMTLARFRVPMVEFPPMQIKLALAGSGHAEKDEVLDAVMRELNLDQPPRPDDAADALAIALTGWFQR

Sequences:

>Translated_154_residues
MRILGIDPGLARVGYGVIDTSNGQQQMLDCGIIRTNPGIDDGVRMVEIASDLRQLIRRWKPQLAAVEKFFFYRSSTTISV
VQARGVIIMTLARFRVPMVEFPPMQIKLALAGSGHAEKDEVLDAVMRELNLDQPPRPDDAADALAIALTGWFQR
>Mature_154_residues
MRILGIDPGLARVGYGVIDTSNGQQQMLDCGIIRTNPGIDDGVRMVEIASDLRQLIRRWKPQLAAVEKFFFYRSSTTISV
VQARGVIIMTLARFRVPMVEFPPMQIKLALAGSGHAEKDEVLDAVMRELNLDQPPRPDDAADALAIALTGWFQR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=136, Percent_Identity=34.5588235294118, Blast_Score=70, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_PROM3 (A2C835)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016910.1
- ProteinModelPortal:   A2C835
- SMR:   A2C835
- STRING:   A2C835
- GeneID:   4777276
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_08941
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   PMQIKLA
- ProtClustDB:   PRK00039
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17108; Mature: 17108

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILGIDPGLARVGYGVIDTSNGQQQMLDCGIIRTNPGIDDGVRMVEIASDLRQLIRRWK
CEEECCCCCHHHHCCCEEECCCCHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHC
PQLAAVEKFFFYRSSTTISVVQARGVIIMTLARFRVPMVEFPPMQIKLALAGSGHAEKDE
HHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHCCCCCCCCCCEEEEEECCCCCCCHHH
VLDAVMRELNLDQPPRPDDAADALAIALTGWFQR
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRILGIDPGLARVGYGVIDTSNGQQQMLDCGIIRTNPGIDDGVRMVEIASDLRQLIRRWK
CEEECCCCCHHHHCCCEEECCCCHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHC
PQLAAVEKFFFYRSSTTISVVQARGVIIMTLARFRVPMVEFPPMQIKLALAGSGHAEKDE
HHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHCCCCCCCCCCEEEEEECCCCCCCHHH
VLDAVMRELNLDQPPRPDDAADALAIALTGWFQR
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA